The Experts below are selected from a list of 54510 Experts worldwide ranked by ideXlab platform

Ashutosh Sharma - One of the best experts on this subject based on the ideXlab platform.

  • Highly sensitive biofunctionalized mesoporous electrospun TiO2 nanofiber based interface for biosensing
    ACS Applied Materials and Interfaces, 2014
    Co-Authors: Koushik Mondal, Ved V. Agrawal, Bansi Dhar Malhotra, Md Azahar Ali, Ashutosh Sharma
    Abstract:

    The surface modified and aligned mesoporous anatase titania nanofiber mats (TiO2-NF) have been fabricated by electrospinning for esterified cholesterol detection by Electrochemical Technique. The electrospinning and porosity of mesoporous TiO2-NF were controlled by use of polyvinylpyrrolidone (PVP) as a sacrificial carrier polymer in the titanium isopropoxide precursor. The mesoporous TiO2-NF of diameters ranging from 30 to 60 nm were obtained by calcination at 470 °C and partially aligned on a rotating drum collector. The functional groups such as -COOH, -CHO etc. were introduced on TiO2-NF surface via oxygen plasma treatment making the surface hydrophilic. Cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) were covalently immobilized on the plasma treated surface of NF (cTiO2-NF) via N-ethyl-N0-(3-dimethylaminopropyl carbodiimide) and N-hydroxysuccinimide (EDC-NHS) chemistry. The high mesoporosity (∼61%) of the fibrous film allowed enhanced loading of the enzyme molecules in the TiO2-NF mat. The ChEt-ChOx/cTiO2-NF-based bioelectrode was used to detect esterified cholesterol using Electrochemical Technique. The high aspect ratio, surface area of aligned TiO2-NF showed excellent voltammetric and catalytic response resulting in improved detection limit (0.49 mM). The results of response studies of this biosensor show excellent sensitivity (181.6 μA/mg dL(-1)/cm(2)) and rapid detection (20 s). This proposed strategy of biomolecule detection is thus a promising platform for the development of miniaturized device for biosensing applications.

H Idrissi - One of the best experts on this subject based on the ideXlab platform.

  • porosity evaluation of protective coatings onto steel through Electrochemical Techniques
    Surface & Coatings Technology, 2000
    Co-Authors: J Creus, H Mazille, H Idrissi
    Abstract:

    Various Electrochemical Techniques, usually used to characterise the corrosion resistance of coated steel, are compared in order to evaluate the porosity of various protective coatings (Al, Ti, TiN or CrN). The porosity significantly influences the corrosion behaviour of the coated steel, tested in saline environment, and some gaps are evident in the porosity values determined from these Techniques. These heterogeneous data are interpreted in terms of intrinsic behaviour of each coating and of galvanic effects between the relevant coating and the substrate. The advantages and drawbacks of each Electrochemical Technique are underlined in correlation with the type of tested coating.

Masayuki Yagi - One of the best experts on this subject based on the ideXlab platform.

Koushik Mondal - One of the best experts on this subject based on the ideXlab platform.

  • Highly sensitive biofunctionalized mesoporous electrospun TiO2 nanofiber based interface for biosensing
    ACS Applied Materials and Interfaces, 2014
    Co-Authors: Koushik Mondal, Ved V. Agrawal, Bansi Dhar Malhotra, Md Azahar Ali, Ashutosh Sharma
    Abstract:

    The surface modified and aligned mesoporous anatase titania nanofiber mats (TiO2-NF) have been fabricated by electrospinning for esterified cholesterol detection by Electrochemical Technique. The electrospinning and porosity of mesoporous TiO2-NF were controlled by use of polyvinylpyrrolidone (PVP) as a sacrificial carrier polymer in the titanium isopropoxide precursor. The mesoporous TiO2-NF of diameters ranging from 30 to 60 nm were obtained by calcination at 470 °C and partially aligned on a rotating drum collector. The functional groups such as -COOH, -CHO etc. were introduced on TiO2-NF surface via oxygen plasma treatment making the surface hydrophilic. Cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) were covalently immobilized on the plasma treated surface of NF (cTiO2-NF) via N-ethyl-N0-(3-dimethylaminopropyl carbodiimide) and N-hydroxysuccinimide (EDC-NHS) chemistry. The high mesoporosity (∼61%) of the fibrous film allowed enhanced loading of the enzyme molecules in the TiO2-NF mat. The ChEt-ChOx/cTiO2-NF-based bioelectrode was used to detect esterified cholesterol using Electrochemical Technique. The high aspect ratio, surface area of aligned TiO2-NF showed excellent voltammetric and catalytic response resulting in improved detection limit (0.49 mM). The results of response studies of this biosensor show excellent sensitivity (181.6 μA/mg dL(-1)/cm(2)) and rapid detection (20 s). This proposed strategy of biomolecule detection is thus a promising platform for the development of miniaturized device for biosensing applications.

J Creus - One of the best experts on this subject based on the ideXlab platform.

  • porosity evaluation of protective coatings onto steel through Electrochemical Techniques
    Surface & Coatings Technology, 2000
    Co-Authors: J Creus, H Mazille, H Idrissi
    Abstract:

    Various Electrochemical Techniques, usually used to characterise the corrosion resistance of coated steel, are compared in order to evaluate the porosity of various protective coatings (Al, Ti, TiN or CrN). The porosity significantly influences the corrosion behaviour of the coated steel, tested in saline environment, and some gaps are evident in the porosity values determined from these Techniques. These heterogeneous data are interpreted in terms of intrinsic behaviour of each coating and of galvanic effects between the relevant coating and the substrate. The advantages and drawbacks of each Electrochemical Technique are underlined in correlation with the type of tested coating.