The Experts below are selected from a list of 15942 Experts worldwide ranked by ideXlab platform
Burkhard Tümmler - One of the best experts on this subject based on the ideXlab platform.
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New approaches in genome analysis by pulsed-field gel Electrophoresis: Application to the analysis of Pseudomonas species.
Molecular microbiology, 1991Co-Authors: D. Grothuesi, Burkhard TümmlerAbstract:Summary Summary A general method for the evaluation of macrorestriction fragment patterns is presented and its applicability to the taxonomy of bacteria is demonstrated for 32 Pseudomonas species. Strains were differentiated at the species and subspecies level by genome size and macrorestriction fragment fingerprints of the chromosome that had been separated on pulsed-field gels. The relatedness of bacteria was ascertained from the similarity of Asnl, Dral, Spel, Sspl or Xbal fragment patterns. In general, the dendrograms calculated from the genome fingerprints corresponded with the phylogenetic classification obtained from phenotypic marker or nucleic acid hybridization analysis, but several exceptions were noted. The techniques and algorithms presented herein are generally applicable to the genome analysis of bacteria, lower eukaryotes, and DNA fragments cloned in yeast artificial chromosomes.
C Bebear - One of the best experts on this subject based on the ideXlab platform.
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molecular typing of mycoplasma pneumoniae strains by pcr based methods and pulsed field gel Electrophoresis Application to french and danish isolates
Epidemiology and Infection, 2000Co-Authors: A Cousinallery, A Charron, B De Barbeyrac, G Fremy, Skov J Jensen, H Renaudin, C BebearAbstract:Restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 153 strains of Mycoplasma pneumoniae isolated in France between 1977 and 1994, and in Denmark between 1962 and 1994, and an additional group of 28 strains isolated from Belgium and Germany between 1990 and 1993. Random amplified polymorphic DNA (RAPD) analysis was tested on French, Belgian and German strains. Both methods separated the strains into two groups corresponding to the two reference strains M129 (group I) and FH (group II), and gave concordant results. When 75 selected strains of different geographical origin were analysed by pulsed-field gel Electrophoresis (PFGE), strains of group II fell into two closely related subgroups, subgroup IIa corresponding to the reference strain FH, and subgroup IIb. Most of the strains isolated in Denmark in the period 1962-86 belonged to group I. Almost all strains isolated in France and Denmark between 1987 and 1988 were from group II, the two subgroups being present. In 1991-3, almost all strains from France as well as Denmark, Germany and Belgium belonged to group I.
Lluis Puignou - One of the best experts on this subject based on the ideXlab platform.
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Determination of polyphenols in Spanish wines by capillary zone Electrophoresis. Application to wine characterization by using chemometrics.
Journal of agricultural and food chemistry, 2012Co-Authors: Helena Franquet-griell, Antonio Checa, Oscar Núñez, Javier Saurina, Santiago Hernández-cassou, Lluis PuignouAbstract:A capillary zone Electrophoresis (CZE) method for the simultaneous determination of 20 polyphenols in wine was developed. The separation was performed using fused-silica capillaries of 75 μm i.d. and a 30 mM sodium tretraborate buffer solution at pH 9.2 with 5% isopropanol as a background electrolyte. A capillary voltage of +25 kV with pressure-assisted (3.5 kPa) separation from minute 18 was applied, thus achieving a total analysis time of 0.990), and run-to-run and day-to-day precisions (RSD values lower than 6.5 and 15.7%, respectively) were established. Three different calibration procedures were evaluated for polyphenol quantitation in wines: external calibration using standards prepared in Milli-Q water, standard addition, and pseudomatrix-matched calibration using wine as a matrix. For a 95% confidence level, no statistical differences were observed, in general, between the t...
D. Grothuesi - One of the best experts on this subject based on the ideXlab platform.
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New approaches in genome analysis by pulsed-field gel Electrophoresis: Application to the analysis of Pseudomonas species.
Molecular microbiology, 1991Co-Authors: D. Grothuesi, Burkhard TümmlerAbstract:Summary Summary A general method for the evaluation of macrorestriction fragment patterns is presented and its applicability to the taxonomy of bacteria is demonstrated for 32 Pseudomonas species. Strains were differentiated at the species and subspecies level by genome size and macrorestriction fragment fingerprints of the chromosome that had been separated on pulsed-field gels. The relatedness of bacteria was ascertained from the similarity of Asnl, Dral, Spel, Sspl or Xbal fragment patterns. In general, the dendrograms calculated from the genome fingerprints corresponded with the phylogenetic classification obtained from phenotypic marker or nucleic acid hybridization analysis, but several exceptions were noted. The techniques and algorithms presented herein are generally applicable to the genome analysis of bacteria, lower eukaryotes, and DNA fragments cloned in yeast artificial chromosomes.
Bi-feng Liu - One of the best experts on this subject based on the ideXlab platform.
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Kinetic and thermodynamic characterization of telomeric G-quadruplex by nonequilibrium capillary Electrophoresis: Application to G-quadruplex/duplex competition.
Analytical chemistry, 2008Co-Authors: Xiaojun Feng, Xin Liu, Qingming Luo, Bi-feng LiuAbstract:In this paper, nonequilibrium capillary Electrophoresis (NECE) was attempted for the first time to investigate a dual equilibrium system, where the intramolecular G-quadruplex folding was in competition with the intermolecular duplex formation. Samples of an equilibrium mixture of human telomeric DNA and its complementary strands were separated in capillaries under nonequilibrium conditions without K+. Polyethylene oxide was added to the running buffer facilitating the separation of single-stranded DNA, duplex, and G-quadruplex. Thus, the folding/unfolding rate constants of the G-quadruplex and the association/dissociation constants of the duplex could be simultaneously derived from the same experiment. Results indicated that the duplex formation induced minimal influence on the G-quadruplex folding. On the basis of the kinetic characterization of the G-quadruplex at varying temperatures, the thermodynamic parameters of the G-quadruplex could also be determined. Thus, the NECE method provided a new avenue...