The Experts below are selected from a list of 1536 Experts worldwide ranked by ideXlab platform

Gabriel A. Monteiro - One of the best experts on this subject based on the ideXlab platform.

  • Electrotransformation of gellan gum producing and non producing pseudomonas elodea strains
    Journal of Applied Microbiology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sacorreia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.

  • Electrotransformation of gellan‐gum producing and non‐producing Pseudomonas elodea strains
    Journal of Applied Bacteriology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sá-correia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.

Isabel Sá-correia - One of the best experts on this subject based on the ideXlab platform.

  • Electrotransformation of gellan‐gum producing and non‐producing Pseudomonas elodea strains
    Journal of Applied Bacteriology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sá-correia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.

Isabel Sacorreia - One of the best experts on this subject based on the ideXlab platform.

  • Electrotransformation of gellan gum producing and non producing pseudomonas elodea strains
    Journal of Applied Microbiology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sacorreia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.

Arsenio M. Fialho - One of the best experts on this subject based on the ideXlab platform.

  • Electrotransformation of gellan gum producing and non producing pseudomonas elodea strains
    Journal of Applied Microbiology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sacorreia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.

  • Electrotransformation of gellan‐gum producing and non‐producing Pseudomonas elodea strains
    Journal of Applied Bacteriology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sá-correia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.

S.j. Ripley - One of the best experts on this subject based on the ideXlab platform.

  • Electrotransformation of gellan gum producing and non producing pseudomonas elodea strains
    Journal of Applied Microbiology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sacorreia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.

  • Electrotransformation of gellan‐gum producing and non‐producing Pseudomonas elodea strains
    Journal of Applied Bacteriology, 1992
    Co-Authors: Gabriel A. Monteiro, Arsenio M. Fialho, S.j. Ripley, Isabel Sá-correia
    Abstract:

    G.A. MONTEIRO, A.M. FIALHO, S.J. RIPLEY AND I.SA -CORREIA. 1992. The Electrotransformation of gellan-gum producing or non-producing strains of Pseudomonas elodea (Gel+ or Gel-) was optimized with respect to growth stage, cell and DNA concentrations and pulse parameters. This technique proved to be a valuable alternative to conjugal mating to search for complementation of gellan mutations for cloning the gellan genes. The Electrotransformation efficiency of Gel+ or Gel- strains was similar. The transformation of smaller plasmids was more efficient than that of larger plasmids, and recombinant plasmids with sizes larger than 35 kb, when extracted from Escherichia coli DH1, were not transformable at detectable frequency. This was partially related to the modification/restriction system active in the recipient cells.