The Experts below are selected from a list of 48 Experts worldwide ranked by ideXlab platform
Andrzej Szutowicz - One of the best experts on this subject based on the ideXlab platform.
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Effect of angiotensin II and Eledoisin on cholinergic neurons in rat hippocampus.
Neuroreport, 1992Co-Authors: L.g. Micossi, M. Tomaszewicz, Hanna Bielarczyk, Danuta Luszawska, Alberto Trognoni, Andrzej SzutowiczAbstract:Angiotensin II and Eledoisin modulate drinking behaviour in rats that is mediated by monoaminergic and cholinergic neurons. In the present study we have shown that combined intracerebroventricular injections of either 0.1 or 1.0 microgram doses of angiotensin and Eledoisin resulted in a decrease of about 25-35% in activities of choline acetyltransferase, ATP-citrate lyase in the hippocampus. In addition, 1 microgram quantities of these peptides depressed activity of carnitine acetyltransferase but did not alter activity of acetylcholinesterase. On the other hand, the application of 0.1 microgram of angiotensin caused no change in activity of monoamine oxidase A, while 1.0 microgram dose brought about its 67% activation. Eledoisin abolished this effect of angiotensin II. These data provide evidence that angiotensin II and Eledoisin evoke non related adaptive changes in cholinergic and monoaminergic neurons of the hippocampus.
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Differential effects of angiotensin II and Eledoisin on monoamine oxidase A and B activities in rat brain.
Journal of neurochemistry, 1991Co-Authors: M. Tomaszewicz, L.g. Micossi, Hanna Bielarczyk, Danuta Luszawska, I. Santarelli, Andrzej SzutowiczAbstract:: Intracerebroventricular injections of angiotensin II caused 108, 62, and 54% increases in monoamine oxidase A activities in rat hippocampus, hypothalamus, and striatum respectively. These activatory effects were abolished by simultaneous injections of Eledoisin. No significant changes of monoamine oxidase B activities were found under the same experimental conditions. Neither angiotensin II nor Eledoisin changed substrate/inhibitor affinities of both isoenzymes. These data indicate that angiotensin II and tachykinin transmitter systems may exert opposite, long-term regulatory effects on monoaminergic neurons in rat brain.
Sudha M. Cowsik - One of the best experts on this subject based on the ideXlab platform.
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Solution Structure of the Tachykinin Peptide Eledoisin
Biophysical journal, 2003Co-Authors: R. Christy Rani Grace, Indu R. Chandrashekar, Sudha M. CowsikAbstract:Both the aqueous and the lipid-induced structure of Eledoisin, an undecapeptide of mollusk origin, have been studied by two-dimensional proton nuclear magnetic resonance spectroscopy and distance geometry calculations. Unambiguous nuclear magnetic resonance assignments of protons have been made with the aid of correlation spectroscopy experiments and nuclear Overhauser effect spectroscopy experiments. The distance constraints obtained from the nuclear magnetic resonance data have been utilized in a distance geometry algorithm to generate a family of structures, which have been refined using restrained energy minimization and dynamics. These data show that, while in water and dimethyl sulfoxide, Eledoisin prefers to be in an extended chain conformation, whereas in the presence of perdeuterated dodecylphosphocholine micelles, a membrane model system, helical conformation is induced in the central core and C-terminal region (K4-M11) of the peptide. N terminus, though less defined, also displays some degree of order and a possible turn structure. The conformation adopted by Eledoisin in the presence of dodecylphosphocholine micelles is similar to the structural motif typical of neurokinin-2 selective agonists and with that reported for kassinin in hydrophobic environment.
M. Tomaszewicz - One of the best experts on this subject based on the ideXlab platform.
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Effect of angiotensin II and Eledoisin on cholinergic neurons in rat hippocampus.
Neuroreport, 1992Co-Authors: L.g. Micossi, M. Tomaszewicz, Hanna Bielarczyk, Danuta Luszawska, Alberto Trognoni, Andrzej SzutowiczAbstract:Angiotensin II and Eledoisin modulate drinking behaviour in rats that is mediated by monoaminergic and cholinergic neurons. In the present study we have shown that combined intracerebroventricular injections of either 0.1 or 1.0 microgram doses of angiotensin and Eledoisin resulted in a decrease of about 25-35% in activities of choline acetyltransferase, ATP-citrate lyase in the hippocampus. In addition, 1 microgram quantities of these peptides depressed activity of carnitine acetyltransferase but did not alter activity of acetylcholinesterase. On the other hand, the application of 0.1 microgram of angiotensin caused no change in activity of monoamine oxidase A, while 1.0 microgram dose brought about its 67% activation. Eledoisin abolished this effect of angiotensin II. These data provide evidence that angiotensin II and Eledoisin evoke non related adaptive changes in cholinergic and monoaminergic neurons of the hippocampus.
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Differential effects of angiotensin II and Eledoisin on monoamine oxidase A and B activities in rat brain.
Journal of neurochemistry, 1991Co-Authors: M. Tomaszewicz, L.g. Micossi, Hanna Bielarczyk, Danuta Luszawska, I. Santarelli, Andrzej SzutowiczAbstract:: Intracerebroventricular injections of angiotensin II caused 108, 62, and 54% increases in monoamine oxidase A activities in rat hippocampus, hypothalamus, and striatum respectively. These activatory effects were abolished by simultaneous injections of Eledoisin. No significant changes of monoamine oxidase B activities were found under the same experimental conditions. Neither angiotensin II nor Eledoisin changed substrate/inhibitor affinities of both isoenzymes. These data indicate that angiotensin II and tachykinin transmitter systems may exert opposite, long-term regulatory effects on monoaminergic neurons in rat brain.
José Barbosa - One of the best experts on this subject based on the ideXlab platform.
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fractionation and characterization of a crude peptide mixture from the synthesis of Eledoisin by liquid chromatography electrospray ionization mass spectrometry
Journal of Chromatography A, 1999Co-Authors: Victoria Sanznebot, I. Toro, José BarbosaAbstract:Abstract Eledoisin is a peptide with complex pharmacological properties and recognized therapeutical application. Peptides of pharmaceutical interest in the molecular mass range of Eledoisin are often synthesized using solid-phase peptide synthesis. This procedure results in complex crudes of reaction that require extensive purification and characterization. In order to avoid long empirical optimization procedures to separate the unwanted products associated with the target peptide Eledoisin from a synthesis crude, the method of linear solvation energy relationships for predicting peptide retention times was applied and the proportion of the organic modifier of the mobile phase used in high-performance liquid chromatography (HPLC) method was optimized. The crude of synthesis was also analyzed by combined HPLC–electrospray ionization mass spectrometry, which allowed rapid and reliable identification of the target peptide and has furnished comprehensive information on other reaction products.
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Fractionation and characterization of a crude peptide mixture from the synthesis of Eledoisin by liquid chromatography–electrospray ionization mass spectrometry
Journal of Chromatography A, 1999Co-Authors: Victoria Sanz-nebot, I. Toro, José BarbosaAbstract:Abstract Eledoisin is a peptide with complex pharmacological properties and recognized therapeutical application. Peptides of pharmaceutical interest in the molecular mass range of Eledoisin are often synthesized using solid-phase peptide synthesis. This procedure results in complex crudes of reaction that require extensive purification and characterization. In order to avoid long empirical optimization procedures to separate the unwanted products associated with the target peptide Eledoisin from a synthesis crude, the method of linear solvation energy relationships for predicting peptide retention times was applied and the proportion of the organic modifier of the mobile phase used in high-performance liquid chromatography (HPLC) method was optimized. The crude of synthesis was also analyzed by combined HPLC–electrospray ionization mass spectrometry, which allowed rapid and reliable identification of the target peptide and has furnished comprehensive information on other reaction products.
L.g. Micossi - One of the best experts on this subject based on the ideXlab platform.
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Effect of angiotensin II and Eledoisin on cholinergic neurons in rat hippocampus.
Neuroreport, 1992Co-Authors: L.g. Micossi, M. Tomaszewicz, Hanna Bielarczyk, Danuta Luszawska, Alberto Trognoni, Andrzej SzutowiczAbstract:Angiotensin II and Eledoisin modulate drinking behaviour in rats that is mediated by monoaminergic and cholinergic neurons. In the present study we have shown that combined intracerebroventricular injections of either 0.1 or 1.0 microgram doses of angiotensin and Eledoisin resulted in a decrease of about 25-35% in activities of choline acetyltransferase, ATP-citrate lyase in the hippocampus. In addition, 1 microgram quantities of these peptides depressed activity of carnitine acetyltransferase but did not alter activity of acetylcholinesterase. On the other hand, the application of 0.1 microgram of angiotensin caused no change in activity of monoamine oxidase A, while 1.0 microgram dose brought about its 67% activation. Eledoisin abolished this effect of angiotensin II. These data provide evidence that angiotensin II and Eledoisin evoke non related adaptive changes in cholinergic and monoaminergic neurons of the hippocampus.
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Differential effects of angiotensin II and Eledoisin on monoamine oxidase A and B activities in rat brain.
Journal of neurochemistry, 1991Co-Authors: M. Tomaszewicz, L.g. Micossi, Hanna Bielarczyk, Danuta Luszawska, I. Santarelli, Andrzej SzutowiczAbstract:: Intracerebroventricular injections of angiotensin II caused 108, 62, and 54% increases in monoamine oxidase A activities in rat hippocampus, hypothalamus, and striatum respectively. These activatory effects were abolished by simultaneous injections of Eledoisin. No significant changes of monoamine oxidase B activities were found under the same experimental conditions. Neither angiotensin II nor Eledoisin changed substrate/inhibitor affinities of both isoenzymes. These data indicate that angiotensin II and tachykinin transmitter systems may exert opposite, long-term regulatory effects on monoaminergic neurons in rat brain.