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Qiliang Chen - One of the best experts on this subject based on the ideXlab platform.

  • effects of copper and cadmium on lipogenic metabolism and metal Element Composition in the javelin goby synechogobius hasta after single and combined exposure
    2014
    Co-Authors: Yufeng Song, Chao Huang, Zhi Luo, Yaxiong Pan, Xu Liu, Qiliang Chen
    Abstract:

    The present study was performed to determine the effects of single and combined exposure of copper (Cu) and cadmium (Cd) on lipogenic metabolism and metal Element Composition of javelin goby Synechogobius hasta. Two hundred and forty uniform-sized S. hasta (initial mean weight 20.3 ± 0.3 g [mean ± SEM throughout]; initial body length 15.2 ± 0.2 cm) were randomly assigned to 12 fiberglass tanks (water volume 300 l) with 20 fish/tank. The fish were exposed to four treatments with different Cu and Cd concentration for 30 days, respectively: (1) control (without extra Cu and Cd addition), (2) Cu (nominal concentrations of 77 μg/l), (3) Cd (79 μg/l), and (4) Cu + Cd (Cu/Cd coexposure). Growth decreased, but hepatosomatic index, viscerosomatic index, and lipid content increased after metal exposure. Staining with Oil Red O and haematoxylin and eosin showed extensive alterations in liver of metals-exposed fish. Metal exposure influenced the accumulation of metal Elements (Cu, Cd, iron, zinc, and manganese) in several tissues (muscle, gill, intestine, liver, and spleen) and increased hepatic 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, malic enzyme, isocitrate dehydrogenase, and fatty acid synthase activities. The results of the present study indicated that the changes in lipogenic metabolism and metal Element Compositions of fish under Cu and Cd coexposure could not be explained by synergism of the addition of the effects observed in singly Cu- or Cd-exposed fish. To our knowledge the present study, for the first time, investigated the effects of Cu and Cd coexposure on hepatic lipogenic metabolism and metal Element Compositions in a wide range of tissues and organs in fish, which provided new evidence for Cu and Cd interactions in fish.

  • effects of copper and cadmium on lipogenic metabolism and metal Element Composition in the javelin goby synechogobius hasta after single and combined exposure
    2014
    Co-Authors: Yufeng Song, Chao Huang, Qiliang Chen
    Abstract:

    The present study was performed to determine the effects of single and combined exposure of copper (Cu) and cadmium (Cd) on lipogenic metabolism and metal Element Composition of javelin goby Synechogobius hasta. Two hundred and forty uniform-sized S. hasta (initial mean weight 20.3 ± 0.3 g [mean ± SEM throughout]; initial body length 15.2 ± 0.2 cm) were randomly assigned to 12 fiberglass tanks (water volume 300 l) with 20 fish/tank. The fish were exposed to four treatments with different Cu and Cd concentration for 30 days, respectively: (1) control (without extra Cu and Cd addition), (2) Cu (nominal concentrations of 77 μg/l), (3) Cd (79 μg/l), and (4) Cu + Cd (Cu/Cd coexposure). Growth decreased, but hepatosomatic index, viscerosomatic index, and lipid content increased after metal exposure. Staining with Oil Red O and haematoxylin and eosin showed extensive alterations in liver of metals-exposed fish. Metal exposure influenced the accumulation of metal Elements (Cu, Cd, iron, zinc, and manganese) in several tissues (muscle, gill, intestine, liver, and spleen) and increased hepatic 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, malic enzyme, isocitrate dehydrogenase, and fatty acid synthase activities. The results of the present study indicated that the changes in lipogenic metabolism and metal Element Compositions of fish under Cu and Cd coexposure could not be explained by synergism of the addition of the effects observed in singly Cu- or Cd-exposed fish. To our knowledge the present study, for the first time, investigated the effects of Cu and Cd coexposure on hepatic lipogenic metabolism and metal Element Compositions in a wide range of tissues and organs in fish, which provided new evidence for Cu and Cd interactions in fish.

  • effects of waterborne chronic copper exposure on hepatic lipid metabolism and metal Element Composition in synechogobius hasta
    2013
    Co-Authors: Qiliang Chen, Yufeng Song, Jialang Zheng, Yanhong Zhao
    Abstract:

    The present study was conducted to determine hepatic lipid metabolism and metal-Element Composition in Synechogobius hasta exposed to waterborne chronic copper (Cu) concentrations of control, 57, and 118 μg Cu/l, respectively, for 30 days. Growth decreased, but hepatosomatic index, viscerosomatic index, and hepatic lipid content increased with increasing waterborne Cu levels. Staining with oil red O showed extensive steatosis in liver of Cu-exposed fish. Cu exposure increased hepatic 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, and malic enzyme activities, whereas fatty acid synthetase, isocitrate dehydrogenase, and carnitine palmitoyltransferases I activities remained unaffected. Cu, zinc, iron, and manganese contents were also changed in several tissues (gill, liver, spleen, gastrointestinal tract, and muscle) in a tissue-, dose-, and time-dependent manner. This was the first study to examine the effects of waterborne Cu exposure on several enzymatic activities mediating hepatic lipogenesis and lipolysis in fish as well as to show that waterborne Cu exposure could enhance the metabolism of lipid synthesis and consequently induce the increase of hepatic lipid deposition in S. hasta.

Ann G. Wintle - One of the best experts on this subject based on the ideXlab platform.

  • trace Element analysis of volcanic glass shards by laser ablation inductively coupled plasma mass spectrometry application to tephrochronological studies
    1994
    Co-Authors: Jennifer A. Westgate, Ronald Fuge, Nicholas J G Pearce, William T Perkins, Ann G. Wintle
    Abstract:

    Abstract The trace-Element Composition of fine-grained (0.25-0.125 mm) volcanic glass shards in distal tephra beds has been determined using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Accuracy is within 15% for most Elements and the precision, as defined by the mean relative standard deviation, is better than 20%. This is very acceptable for tephrochronological studies. The very small amount of glass ablated (∼80 μg) and the short time involved in a single analysis (∼2 min) means that contamination can be easily recognized in the presence of replicate analyses and a detailed definition of trace-Element Compositional variability of the glass can be obtained. The need to use another trace-Element analytical method to obtain the internal standard, Ce in this study, is a disadvantage but preliminary work in progress suggests that 57Fe can be used for this purpose. This would obviate the need to use another trace-Element method because the major-Element Composition of glass shards, as determined by electron microprobe analysis, constitutes a routine part of tephra characterization studies.

Yufeng Song - One of the best experts on this subject based on the ideXlab platform.

  • effects of copper and cadmium on lipogenic metabolism and metal Element Composition in the javelin goby synechogobius hasta after single and combined exposure
    2014
    Co-Authors: Yufeng Song, Chao Huang, Zhi Luo, Yaxiong Pan, Xu Liu, Qiliang Chen
    Abstract:

    The present study was performed to determine the effects of single and combined exposure of copper (Cu) and cadmium (Cd) on lipogenic metabolism and metal Element Composition of javelin goby Synechogobius hasta. Two hundred and forty uniform-sized S. hasta (initial mean weight 20.3 ± 0.3 g [mean ± SEM throughout]; initial body length 15.2 ± 0.2 cm) were randomly assigned to 12 fiberglass tanks (water volume 300 l) with 20 fish/tank. The fish were exposed to four treatments with different Cu and Cd concentration for 30 days, respectively: (1) control (without extra Cu and Cd addition), (2) Cu (nominal concentrations of 77 μg/l), (3) Cd (79 μg/l), and (4) Cu + Cd (Cu/Cd coexposure). Growth decreased, but hepatosomatic index, viscerosomatic index, and lipid content increased after metal exposure. Staining with Oil Red O and haematoxylin and eosin showed extensive alterations in liver of metals-exposed fish. Metal exposure influenced the accumulation of metal Elements (Cu, Cd, iron, zinc, and manganese) in several tissues (muscle, gill, intestine, liver, and spleen) and increased hepatic 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, malic enzyme, isocitrate dehydrogenase, and fatty acid synthase activities. The results of the present study indicated that the changes in lipogenic metabolism and metal Element Compositions of fish under Cu and Cd coexposure could not be explained by synergism of the addition of the effects observed in singly Cu- or Cd-exposed fish. To our knowledge the present study, for the first time, investigated the effects of Cu and Cd coexposure on hepatic lipogenic metabolism and metal Element Compositions in a wide range of tissues and organs in fish, which provided new evidence for Cu and Cd interactions in fish.

  • effects of copper and cadmium on lipogenic metabolism and metal Element Composition in the javelin goby synechogobius hasta after single and combined exposure
    2014
    Co-Authors: Yufeng Song, Chao Huang, Qiliang Chen
    Abstract:

    The present study was performed to determine the effects of single and combined exposure of copper (Cu) and cadmium (Cd) on lipogenic metabolism and metal Element Composition of javelin goby Synechogobius hasta. Two hundred and forty uniform-sized S. hasta (initial mean weight 20.3 ± 0.3 g [mean ± SEM throughout]; initial body length 15.2 ± 0.2 cm) were randomly assigned to 12 fiberglass tanks (water volume 300 l) with 20 fish/tank. The fish were exposed to four treatments with different Cu and Cd concentration for 30 days, respectively: (1) control (without extra Cu and Cd addition), (2) Cu (nominal concentrations of 77 μg/l), (3) Cd (79 μg/l), and (4) Cu + Cd (Cu/Cd coexposure). Growth decreased, but hepatosomatic index, viscerosomatic index, and lipid content increased after metal exposure. Staining with Oil Red O and haematoxylin and eosin showed extensive alterations in liver of metals-exposed fish. Metal exposure influenced the accumulation of metal Elements (Cu, Cd, iron, zinc, and manganese) in several tissues (muscle, gill, intestine, liver, and spleen) and increased hepatic 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, malic enzyme, isocitrate dehydrogenase, and fatty acid synthase activities. The results of the present study indicated that the changes in lipogenic metabolism and metal Element Compositions of fish under Cu and Cd coexposure could not be explained by synergism of the addition of the effects observed in singly Cu- or Cd-exposed fish. To our knowledge the present study, for the first time, investigated the effects of Cu and Cd coexposure on hepatic lipogenic metabolism and metal Element Compositions in a wide range of tissues and organs in fish, which provided new evidence for Cu and Cd interactions in fish.

  • effects of waterborne chronic copper exposure on hepatic lipid metabolism and metal Element Composition in synechogobius hasta
    2013
    Co-Authors: Qiliang Chen, Yufeng Song, Jialang Zheng, Yanhong Zhao
    Abstract:

    The present study was conducted to determine hepatic lipid metabolism and metal-Element Composition in Synechogobius hasta exposed to waterborne chronic copper (Cu) concentrations of control, 57, and 118 μg Cu/l, respectively, for 30 days. Growth decreased, but hepatosomatic index, viscerosomatic index, and hepatic lipid content increased with increasing waterborne Cu levels. Staining with oil red O showed extensive steatosis in liver of Cu-exposed fish. Cu exposure increased hepatic 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, and malic enzyme activities, whereas fatty acid synthetase, isocitrate dehydrogenase, and carnitine palmitoyltransferases I activities remained unaffected. Cu, zinc, iron, and manganese contents were also changed in several tissues (gill, liver, spleen, gastrointestinal tract, and muscle) in a tissue-, dose-, and time-dependent manner. This was the first study to examine the effects of waterborne Cu exposure on several enzymatic activities mediating hepatic lipogenesis and lipolysis in fish as well as to show that waterborne Cu exposure could enhance the metabolism of lipid synthesis and consequently induce the increase of hepatic lipid deposition in S. hasta.

Isabel P. Montañez - One of the best experts on this subject based on the ideXlab platform.

  • differentiating the bishop ash bed and related tephra layers by Elemental based similarity coefficients of volcanic glass shards using solution inductively coupled plasma mass spectrometry s icp ms
    2007
    Co-Authors: Jeffrey R Knott, Andrei M Sarnawojcicki, Isabel P. Montañez
    Abstract:

    Volcanic glass samples from the same volcanic center (intra-source) often have a similar major-Element Composition. Thus, it can be difficult to distinguish between individual tephra layers, particularly when using similarity coefficients calculated from electron microprobe major-Element measurements. Minor/trace Element concentrations in glass can be determined by solution inductively coupled plasma mass spectrometry (S-ICP-MS), but have not been shown as suitable for use in large tephrochronologic databases. Here, we present minor/trace-Element concentrations measured by S-ICP-MS and compare these data by similarity coefficients, the method commonly used in large databases. Trial samples from the Bishop Tuff, the upper and lower tuffs of Glass Mountain and the tuffs of Mesquite Spring suites from eastern California, USA, which have an indistinguishable major-Element Composition, were analyzed using S-ICP-MS. The resulting minor/trace Element similarity coefficients clearly separated the suites of tephra layers and, in most cases, individual tephra layers within each suite. Comparisons with previous instrumental neutron activation analysis (INAA) Elemental measurements were marginally successful. This is important step toward quantitative correlation in large tephrochronologic databases to achieve definitive identification of volcanic glass samples and for high-resolution age determinations.

Jennifer A. Westgate - One of the best experts on this subject based on the ideXlab platform.

  • trace Element analysis of volcanic glass shards by laser ablation inductively coupled plasma mass spectrometry application to tephrochronological studies
    1994
    Co-Authors: Jennifer A. Westgate, Ronald Fuge, Nicholas J G Pearce, William T Perkins, Ann G. Wintle
    Abstract:

    Abstract The trace-Element Composition of fine-grained (0.25-0.125 mm) volcanic glass shards in distal tephra beds has been determined using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Accuracy is within 15% for most Elements and the precision, as defined by the mean relative standard deviation, is better than 20%. This is very acceptable for tephrochronological studies. The very small amount of glass ablated (∼80 μg) and the short time involved in a single analysis (∼2 min) means that contamination can be easily recognized in the presence of replicate analyses and a detailed definition of trace-Element Compositional variability of the glass can be obtained. The need to use another trace-Element analytical method to obtain the internal standard, Ce in this study, is a disadvantage but preliminary work in progress suggests that 57Fe can be used for this purpose. This would obviate the need to use another trace-Element method because the major-Element Composition of glass shards, as determined by electron microprobe analysis, constitutes a routine part of tephra characterization studies.