The Experts below are selected from a list of 45489 Experts worldwide ranked by ideXlab platform
Harris G Fienberg - One of the best experts on this subject based on the ideXlab platform.
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a platinum based covalent viability reagent for single cell mass cytometry
Cytometry Part A, 2012Co-Authors: Harris G Fienberg, Erin F Simonds, Wendy J Fantl, Garry P Nolan, Bernd BodenmillerAbstract:In fluorescence-based flow cytometry, cellular viability is determined with membrane-impermeable fluorescent reagents that specifically enter and label plasma membrane-compromised nonviable cells. A recent technological advance in flow cytometry uses antibodies conjugated to Elemental Metal isotopes, rather than to fluorophores, to allow signal detection by atomic mass spectrometry. Unhampered by the limitations of overlapping emission fluorescence, mass cytometry increases the number of parameters that can be measured in single cells. However, mass cytometry is unable to take advantage of current fluorescent viability dyes. An alternative methodology was therefore developed here in which the platinum-containing chemotherapy drug cisplatin was used to resolve live and dead cells by mass cytometry. In a 1-min incubation step, cisplatin preferentially labeled nonviable cells from both adherent and suspension cultures, resulting in a platinum signal quantifiable by mass cytometry. This protocol was compatible with established sample processing steps for intracellular cytometry. Furthermore, the live/dead ratios were comparable between mass- and fluorescence-based cytometry. Importantly, although cisplatin is a known DNA-damaging agent, a 1-min "pulse" of cisplatin did not induce observable DNA damage or apoptotic responses even within 6-h post-exposure. Cisplatin can therefore be used as a viability reagent for a wide range of mass cytometry protocols.
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a platinum based covalent viability reagent for single cell mass cytometry
Cytometry Part A, 2012Co-Authors: Harris G Fienberg, Erin F Simonds, Wendy J Fantl, Garry P Nola, Ernd OdenmilleAbstract:In fluorescence-based flow cytometry, cellular viability is determined with membrane-impermeable fluorescent reagents that specifically enter and label plasma membrane-compromised nonviable cells. A recent technological advance in flow cytometry uses antibodies conjugated to Elemental Metal isotopes, rather than to fluorophores, to allow signal detection by atomic mass spectrometry. Unhampered by the limitations of overlapping emission fluorescence, mass cytometry increases the number of parameters that can be measured in single cells. However, mass cytometry is unable to take advantage of current fluorescent viability dyes. An alternative methodology was therefore developed here in which the platinum-containing chemotherapy drug cisplatin was used to resolve live and dead cells by mass cytometry. In a 1-min incubation step, cisplatin preferentially labeled nonviable cells from both adherent and suspension cultures, resulting in a platinum signal quantifiable by mass cytometry. This protocol was compatible with established sample processing steps for intracellular cytometry. Furthermore, the live/dead ratios were comparable between mass- and fluorescence-based cytometry. Importantly, although cisplatin is a known DNA-damaging agent, a 1-min “pulse” of cisplatin did not induce observable DNA damage or apoptotic responses even within 6-h post-exposure. Cisplatin can therefore be used as a viability reagent for a wide range of mass cytometry protocols. © 2012 International Society for Advancement of Cytometry
Raffaele Mezzenga - One of the best experts on this subject based on the ideXlab platform.
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Amyloid–carbon hybrid membranes for universal water purification
Nature Nanotechnology, 2016Co-Authors: Sreenath Bolisetty, Raffaele MezzengaAbstract:Industrial development, energy production and mining have led to dramatically increased levels of environmental pollutants such as heavy Metal ions, Metal cyanides and nuclear waste. Current technologies for purifying contaminated waters are typically expensive and ion specific, and there is therefore a significant need for new approaches. Here, we report inexpensive hybrid membranes made from protein amyloid fibrils and activated porous carbon that can be used to remove heavy Metal ions and radioactive waste from water. During filtration, the concentration of heavy Metal ions drops by three to five orders of magnitude per passage and the process can be repeated numerous times. Notably, their efficiency remains unaltered when filtering several ions simultaneously. The performance of the membrane is enabled by the ability of the amyloids to selectively absorb heavy Metal pollutants from solutions. We also show that our membranes can be used to recycle valuable heavy Metal contaminants by thermally reducing ions trapped in saturated membranes, leading to the creation of Elemental Metal nanoparticles and films.Hybrid membranes made from protein amyloid fibrils and activated porous carbon can be used to remove heavy Metal ions and radioactive waste from water.
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amyloid carbon hybrid membranes for universal water purification
Nature Nanotechnology, 2016Co-Authors: Sreenath Bolisetty, Raffaele MezzengaAbstract:Industrial development, energy production and mining have led to dramatically increased levels of environmental pollutants such as heavy Metal ions, Metal cyanides and nuclear waste. Current technologies for purifying contaminated waters are typically expensive and ion specific, and there is therefore a significant need for new approaches. Here, we report inexpensive hybrid membranes made from protein amyloid fibrils and activated porous carbon that can be used to remove heavy Metal ions and radioactive waste from water. During filtration, the concentration of heavy Metal ions drops by three to five orders of magnitude per passage and the process can be repeated numerous times. Notably, their efficiency remains unaltered when filtering several ions simultaneously. The performance of the membrane is enabled by the ability of the amyloids to selectively absorb heavy Metal pollutants from solutions. We also show that our membranes can be used to recycle valuable heavy Metal contaminants by thermally reducing ions trapped in saturated membranes, leading to the creation of Elemental Metal nanoparticles and films. Hybrid membranes made from protein amyloid fibrils and activated porous carbon can be used to remove heavy Metal ions and radioactive waste from water.
Ernd Odenmille - One of the best experts on this subject based on the ideXlab platform.
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a platinum based covalent viability reagent for single cell mass cytometry
Cytometry Part A, 2012Co-Authors: Harris G Fienberg, Erin F Simonds, Wendy J Fantl, Garry P Nola, Ernd OdenmilleAbstract:In fluorescence-based flow cytometry, cellular viability is determined with membrane-impermeable fluorescent reagents that specifically enter and label plasma membrane-compromised nonviable cells. A recent technological advance in flow cytometry uses antibodies conjugated to Elemental Metal isotopes, rather than to fluorophores, to allow signal detection by atomic mass spectrometry. Unhampered by the limitations of overlapping emission fluorescence, mass cytometry increases the number of parameters that can be measured in single cells. However, mass cytometry is unable to take advantage of current fluorescent viability dyes. An alternative methodology was therefore developed here in which the platinum-containing chemotherapy drug cisplatin was used to resolve live and dead cells by mass cytometry. In a 1-min incubation step, cisplatin preferentially labeled nonviable cells from both adherent and suspension cultures, resulting in a platinum signal quantifiable by mass cytometry. This protocol was compatible with established sample processing steps for intracellular cytometry. Furthermore, the live/dead ratios were comparable between mass- and fluorescence-based cytometry. Importantly, although cisplatin is a known DNA-damaging agent, a 1-min “pulse” of cisplatin did not induce observable DNA damage or apoptotic responses even within 6-h post-exposure. Cisplatin can therefore be used as a viability reagent for a wide range of mass cytometry protocols. © 2012 International Society for Advancement of Cytometry
Bernd Bodenmiller - One of the best experts on this subject based on the ideXlab platform.
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a platinum based covalent viability reagent for single cell mass cytometry
Cytometry Part A, 2012Co-Authors: Harris G Fienberg, Erin F Simonds, Wendy J Fantl, Garry P Nolan, Bernd BodenmillerAbstract:In fluorescence-based flow cytometry, cellular viability is determined with membrane-impermeable fluorescent reagents that specifically enter and label plasma membrane-compromised nonviable cells. A recent technological advance in flow cytometry uses antibodies conjugated to Elemental Metal isotopes, rather than to fluorophores, to allow signal detection by atomic mass spectrometry. Unhampered by the limitations of overlapping emission fluorescence, mass cytometry increases the number of parameters that can be measured in single cells. However, mass cytometry is unable to take advantage of current fluorescent viability dyes. An alternative methodology was therefore developed here in which the platinum-containing chemotherapy drug cisplatin was used to resolve live and dead cells by mass cytometry. In a 1-min incubation step, cisplatin preferentially labeled nonviable cells from both adherent and suspension cultures, resulting in a platinum signal quantifiable by mass cytometry. This protocol was compatible with established sample processing steps for intracellular cytometry. Furthermore, the live/dead ratios were comparable between mass- and fluorescence-based cytometry. Importantly, although cisplatin is a known DNA-damaging agent, a 1-min "pulse" of cisplatin did not induce observable DNA damage or apoptotic responses even within 6-h post-exposure. Cisplatin can therefore be used as a viability reagent for a wide range of mass cytometry protocols.
Myung-hwan Whangbo - One of the best experts on this subject based on the ideXlab platform.
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continual injection of photoinduced electrons stabilizing surface plasmon resonance of non Elemental Metal plasmonic photocatalyst cds wo3 x for efficient hydrogen generation
Applied Catalysis B-environmental, 2018Co-Authors: Zaizhu Lou, Mingshan Zhu, Xianguang Yang, Yao Zhang, Myung-hwan Whangbo, Baibiao HuangAbstract:Abstract To remedy the instability problem of the non-Elemental-Metal (NEM) plasmonic photocatalyst WO3−x in aqueous solution, a novel strategy of photoinduced electron injection was applied to construct CdS/WO3−x hetereostructures by growing WO3−x on semiconductor CdS nanowires. Under visible/near-infrared light irradiation, the NEM plasmonic CdS/WO3−x nanowires are highly stable and exhibit a much higher activity (1.60 mmol g−1 h−1) than do plasmonic WO3−x (negligible amount) and semiconductor CdS (0.53 mmol g−1 h−1) in hydrogen generation. Wavelength dependent photocatalytic performance and Single-particle PL study demonstrate that photo-excited electrons on CdS continually inject into conduction band of WO3−x, so that the surface plasmon resonance (SPR) of the NEM plasmonic photocatalyst WO3−x is sustained while hot electrons generated by the SPR excitation are consumed for hydrogen generation.
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Continual injection of photoinduced electrons stabilizing surface plasmon resonance of non-Elemental-Metal plasmonic photocatalyst CdS/WO3−x for efficient hydrogen generation
Applied Catalysis B: Environmental, 2018Co-Authors: Zaizhu Lou, Mingshan Zhu, Xianguang Yang, Yao Zhang, Myung-hwan Whangbo, Baibiao HuangAbstract:Abstract To remedy the instability problem of the non-Elemental-Metal (NEM) plasmonic photocatalyst WO3−x in aqueous solution, a novel strategy of photoinduced electron injection was applied to construct CdS/WO3−x hetereostructures by growing WO3−x on semiconductor CdS nanowires. Under visible/near-infrared light irradiation, the NEM plasmonic CdS/WO3−x nanowires are highly stable and exhibit a much higher activity (1.60 mmol g−1 h−1) than do plasmonic WO3−x (negligible amount) and semiconductor CdS (0.53 mmol g−1 h−1) in hydrogen generation. Wavelength dependent photocatalytic performance and Single-particle PL study demonstrate that photo-excited electrons on CdS continually inject into conduction band of WO3−x, so that the surface plasmon resonance (SPR) of the NEM plasmonic photocatalyst WO3−x is sustained while hot electrons generated by the SPR excitation are consumed for hydrogen generation.