The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Jian Wang - One of the best experts on this subject based on the ideXlab platform.

  • Embryo Implantation is closely associated with dynamic expression of proprotein convertase 5 6 in the rabbit uterus
    Reproductive Biology and Endocrinology, 2011
    Co-Authors: Peter K Nicholls, Sophea Heng, Ying Li, Jian Wang
    Abstract:

    Background Proprotein convertase 5/6 (PC5/6) is critical for Embryo Implantation in women, regulating both uterine epithelial receptivity and stromal cell decidualization. PC5/6 is likewise essential for Implantation in mice, but involved only in decidualization. An alternative animal model is required to address the function of PC5/6 in the uterine epithelium. This study aimed to establish whether PC5/6 is associated with Embryo Implantation in rabbits.

  • 166 Embryo Implantation is associated with specific expression of proprotein convertase 6 in the rabbit uterus
    Reproduction Fertility and Development, 2010
    Co-Authors: Ying Li, Peter K Nicholls, Sophea Heng, Jian Wang
    Abstract:

    Proprotein convertase 5/6 (PC6) is a member of the proprotein convertase family that endoproteolytically cleave latent precursor proteins into their biologically active state. We have previously demonstrated that endometrial PC6 is critical for Embryo Implantation in mice and primates, including human. PC6 regulates the endometrial physiology specifically at Implantation in association with epithelial differentiation during the establishment of endometrial receptivity (in human and monkey) and stromal cell decidualization (in the mouse, human and monkey). PC6 was further confirmed to be a unique PC member that is tightly regulated in the endometrium in relation to Implantation. Our further studies (unpublished) suggest that PC6 regulates adhesion molecules in the endometrial epithelium for Implantation in women. It is known that between the mouse and human, the endometrial stroma-mediated responses are similar whereas the epithelial cells behave differently. Because PC6 regulates primarily the stromal component (decidualization) in the mouse, in vivo mouse models are critical to investigate the roles of PC6 in decidualization. To address the function of PC6 in endometrial epithelium, non-mouse models relevant to human Implantation are required. The rabbit is regarded as an excellent model to study the molecular events of Embryo adhesion and attachment. The current study aimed to determine the expression pattern and localisation of PC6 in the rabbit uterus during early pregnancy. Quantitative RT-PCR analysis showed that PC6 mRNA expression was dynamically up-regulated in the rabbit uterus immediately prior to Implantation. Western blotting and immunohistochemical analyses demonstrated that PC6 protein was predominantly localised to the basal glands throughout pregnancy, and up-regulated specifically in the epithelium at the Embryo attachment site. These findings suggest that PC6 may play an essential role in rabbit Implantation, and that the rabbit is a useful animal model to investigate the function of PC6 during Embryo attachment.

Sophea Heng - One of the best experts on this subject based on the ideXlab platform.

  • proprotein convertase 5 6 is critical for Embryo Implantation in women regulating receptivity by cleaving ebp50 modulating ezrin binding and membrane cytoskeletal interactions
    Endocrinology, 2011
    Co-Authors: Sophea Heng, Ying Li, Carlos Simon, Ana Cervero, Andrew N Stephens, Jin Zhang, Sarah Paule, Adam Rainczuk, Harmeet Singh, Alicia Quinonero
    Abstract:

    Establishment of endometrial receptivity is vital for successful Embryo Implantation; its failure causes infertility. Epithelial receptivity acquisition involves dramatic structural changes in the plasma membrane and cytoskeleton. Proprotein convertase 5/6 (PC6), a serine protease of the proprotein convertase (PC) family, is up-regulated in the human endometrium specifically at the time of epithelial receptivity and stromal cell decidualization. PC6 is the only PC member tightly regulated in this manner. The current study addressed the importance and mechanisms of PC6 action in regulating receptivity in women. PC6 was dysregulated in the endometrial epithelium during the window of Implantation in infertile women of three demographically different cohorts. Its critical role in receptivity was evidenced by a significant reduction in mouse blastocyst attachment of endometrial epithelial cells after PC6 knockdown by small interfering RNA. Using a proteomic approach, we discovered that PC6 cleaved the key scaf...

  • Embryo Implantation is closely associated with dynamic expression of proprotein convertase 5 6 in the rabbit uterus
    Reproductive Biology and Endocrinology, 2011
    Co-Authors: Peter K Nicholls, Sophea Heng, Ying Li, Jian Wang
    Abstract:

    Background Proprotein convertase 5/6 (PC5/6) is critical for Embryo Implantation in women, regulating both uterine epithelial receptivity and stromal cell decidualization. PC5/6 is likewise essential for Implantation in mice, but involved only in decidualization. An alternative animal model is required to address the function of PC5/6 in the uterine epithelium. This study aimed to establish whether PC5/6 is associated with Embryo Implantation in rabbits.

  • 166 Embryo Implantation is associated with specific expression of proprotein convertase 6 in the rabbit uterus
    Reproduction Fertility and Development, 2010
    Co-Authors: Ying Li, Peter K Nicholls, Sophea Heng, Jian Wang
    Abstract:

    Proprotein convertase 5/6 (PC6) is a member of the proprotein convertase family that endoproteolytically cleave latent precursor proteins into their biologically active state. We have previously demonstrated that endometrial PC6 is critical for Embryo Implantation in mice and primates, including human. PC6 regulates the endometrial physiology specifically at Implantation in association with epithelial differentiation during the establishment of endometrial receptivity (in human and monkey) and stromal cell decidualization (in the mouse, human and monkey). PC6 was further confirmed to be a unique PC member that is tightly regulated in the endometrium in relation to Implantation. Our further studies (unpublished) suggest that PC6 regulates adhesion molecules in the endometrial epithelium for Implantation in women. It is known that between the mouse and human, the endometrial stroma-mediated responses are similar whereas the epithelial cells behave differently. Because PC6 regulates primarily the stromal component (decidualization) in the mouse, in vivo mouse models are critical to investigate the roles of PC6 in decidualization. To address the function of PC6 in endometrial epithelium, non-mouse models relevant to human Implantation are required. The rabbit is regarded as an excellent model to study the molecular events of Embryo adhesion and attachment. The current study aimed to determine the expression pattern and localisation of PC6 in the rabbit uterus during early pregnancy. Quantitative RT-PCR analysis showed that PC6 mRNA expression was dynamically up-regulated in the rabbit uterus immediately prior to Implantation. Western blotting and immunohistochemical analyses demonstrated that PC6 protein was predominantly localised to the basal glands throughout pregnancy, and up-regulated specifically in the epithelium at the Embryo attachment site. These findings suggest that PC6 may play an essential role in rabbit Implantation, and that the rabbit is a useful animal model to investigate the function of PC6 during Embryo attachment.

Ying Li - One of the best experts on this subject based on the ideXlab platform.

  • proprotein convertase 5 6 is critical for Embryo Implantation in women regulating receptivity by cleaving ebp50 modulating ezrin binding and membrane cytoskeletal interactions
    Endocrinology, 2011
    Co-Authors: Sophea Heng, Ying Li, Carlos Simon, Ana Cervero, Andrew N Stephens, Jin Zhang, Sarah Paule, Adam Rainczuk, Harmeet Singh, Alicia Quinonero
    Abstract:

    Establishment of endometrial receptivity is vital for successful Embryo Implantation; its failure causes infertility. Epithelial receptivity acquisition involves dramatic structural changes in the plasma membrane and cytoskeleton. Proprotein convertase 5/6 (PC6), a serine protease of the proprotein convertase (PC) family, is up-regulated in the human endometrium specifically at the time of epithelial receptivity and stromal cell decidualization. PC6 is the only PC member tightly regulated in this manner. The current study addressed the importance and mechanisms of PC6 action in regulating receptivity in women. PC6 was dysregulated in the endometrial epithelium during the window of Implantation in infertile women of three demographically different cohorts. Its critical role in receptivity was evidenced by a significant reduction in mouse blastocyst attachment of endometrial epithelial cells after PC6 knockdown by small interfering RNA. Using a proteomic approach, we discovered that PC6 cleaved the key scaf...

  • Embryo Implantation is closely associated with dynamic expression of proprotein convertase 5 6 in the rabbit uterus
    Reproductive Biology and Endocrinology, 2011
    Co-Authors: Peter K Nicholls, Sophea Heng, Ying Li, Jian Wang
    Abstract:

    Background Proprotein convertase 5/6 (PC5/6) is critical for Embryo Implantation in women, regulating both uterine epithelial receptivity and stromal cell decidualization. PC5/6 is likewise essential for Implantation in mice, but involved only in decidualization. An alternative animal model is required to address the function of PC5/6 in the uterine epithelium. This study aimed to establish whether PC5/6 is associated with Embryo Implantation in rabbits.

  • 166 Embryo Implantation is associated with specific expression of proprotein convertase 6 in the rabbit uterus
    Reproduction Fertility and Development, 2010
    Co-Authors: Ying Li, Peter K Nicholls, Sophea Heng, Jian Wang
    Abstract:

    Proprotein convertase 5/6 (PC6) is a member of the proprotein convertase family that endoproteolytically cleave latent precursor proteins into their biologically active state. We have previously demonstrated that endometrial PC6 is critical for Embryo Implantation in mice and primates, including human. PC6 regulates the endometrial physiology specifically at Implantation in association with epithelial differentiation during the establishment of endometrial receptivity (in human and monkey) and stromal cell decidualization (in the mouse, human and monkey). PC6 was further confirmed to be a unique PC member that is tightly regulated in the endometrium in relation to Implantation. Our further studies (unpublished) suggest that PC6 regulates adhesion molecules in the endometrial epithelium for Implantation in women. It is known that between the mouse and human, the endometrial stroma-mediated responses are similar whereas the epithelial cells behave differently. Because PC6 regulates primarily the stromal component (decidualization) in the mouse, in vivo mouse models are critical to investigate the roles of PC6 in decidualization. To address the function of PC6 in endometrial epithelium, non-mouse models relevant to human Implantation are required. The rabbit is regarded as an excellent model to study the molecular events of Embryo adhesion and attachment. The current study aimed to determine the expression pattern and localisation of PC6 in the rabbit uterus during early pregnancy. Quantitative RT-PCR analysis showed that PC6 mRNA expression was dynamically up-regulated in the rabbit uterus immediately prior to Implantation. Western blotting and immunohistochemical analyses demonstrated that PC6 protein was predominantly localised to the basal glands throughout pregnancy, and up-regulated specifically in the epithelium at the Embryo attachment site. These findings suggest that PC6 may play an essential role in rabbit Implantation, and that the rabbit is a useful animal model to investigate the function of PC6 during Embryo attachment.

Ariel Revel - One of the best experts on this subject based on the ideXlab platform.

  • micrornas are associated with human Embryo Implantation defects
    Human Reproduction, 2011
    Co-Authors: Ariel Revel, Hanna Achache, Juliet Stevens, Yoav Smith, Reuven Reich
    Abstract:

    background: Repeated Implantation failure (RIF) is a major problem encountered in IVF. We have previously reported that RIF-IVF patients have a different endometrial gene expression profile during the window of Implantation. Considering microRNA (miRNA) function in post-transcriptional regulation of gene expression, the aim of the study was to evaluate the involvement of miRNA in defects of endometrial receptivity. methods: We used TaqMan miRNA array cards to identify the miRNAs differentially expressed in the secretory endometrium of RIF-IVF patients when compared with fertile women, and bioinformatics tools to identify their predicted targets and the molecular networks they may affect. results: Comparing miRNA expression profiles, we identified 13 miRNAs, differentially expressed in RIF endometrial samples, that putatively regulate the expression of 3800 genes. We found that 10 miRNAs were overexpressed (including miR 145, 23b and 99a) and 3 were underexpressed. Using our previous gene expression analysis, we paralleled miRNA –mRNA expression profiling. By this means, we identified novel and previously characterized miRNA-regulated molecular pathways such as adherens junctions, cell adhesion molecules, Wnt-signaling, p53 signaling and cell cycle pathways. Consistent with the miRNA-predicted targets, mRNA levels of N-cadherin, H2AFX, netrin-4 and secreted frizzled-related protein-4, belonging to the cell adhesion molecules, Wnt signaling and cell cycle pathways were lower in RIF-IVF patients. conclusions: To our knowledge, this is the first study to evaluate the differential expression of miRNAs in the secretory endometrium of RIF-IVF patients. We suggest that the RIF-associated miRNAs could be exploited as new candidates for diagnosis and treatment of Embryo Implantation failures.

  • endometrial receptivity markers the journey to successful Embryo Implantation
    Human Reproduction Update, 2006
    Co-Authors: Hanna Achache, Ariel Revel
    Abstract:

    Human Embryo Implantation is a three-stage process (apposition, adhesion and invasion) involving synchronized crosstalk between a receptive endometrium and a functional blastocyst. This ovarian steroid-dependant phenomenon can only take place during the window of Implantation, a self-limited period of endometrial receptivity spanning between days 20 and 24 of the menstrual cycle. Implantation involves a complex sequence of signalling events, consisting in the acquisition of adhesion ligands together with the loss of inhibitory components, which are crucial to the establishment of pregnancy. Histological evaluation, now considered to add little clinically significant information, should be replaced by functional assessment of endometrial receptivity. A large number of molecular mediators have been identified to date, including adhesion molecules, cytokines, growth factors, lipids and others. Thus, endometrial biopsy samples can be used to identify molecules associated with uterine receptivity to obtain a better insight into human Implantation. In addition, development of functional in vitro systems to study Embryo–uterine interactions will lead to better definition of the interactions existing between the molecules involved in this process. The purpose of this review was not only to describe the different players of the Implantation process but also to try to portray the relationship between these factors and their timing in the process of uterine receptivity.

  • endometrial receptivity markers the journey to successful Embryo Implantation
    Human Reproduction Update, 2006
    Co-Authors: Hanna Achache, Ariel Revel
    Abstract:

    Human Embryo Implantation is a three-stage process (apposition, adhesion and invasion) involving synchronized crosstalk between a receptive endometrium and a functional blastocyst. This ovarian steroid-dependent phenomenon can only take place during the window of Implantation, a self-limited period of endometrial receptivity spanning between days 20 and 24 of the menstrual cycle. Implantation involves a complex sequence of signalling events, consisting in the acquisition of adhesion ligands together with the loss of inhibitory components, which are crucial to the establishment of pregnancy. Histological evaluation, now considered to add little clinically significant information, should be replaced by functional assessment of endometrial receptivity. A large number of molecular mediators have been identified to date, including adhesion molecules, cytokines, growth factors, lipids and others. Thus, endometrial biopsy samples can be used to identify molecules associated with uterine receptivity to obtain a better insight into human Implantation. In addition, development of functional in vitro systems to study Embryo-uterine interactions will lead to better definition of the interactions existing between the molecules involved in this process. The purpose of this review was not only to describe the different players of the Implantation process but also to try to portray the relationship between these factors and their timing in the process of uterine receptivity.

Peter K Nicholls - One of the best experts on this subject based on the ideXlab platform.

  • Embryo Implantation is closely associated with dynamic expression of proprotein convertase 5 6 in the rabbit uterus
    Reproductive Biology and Endocrinology, 2011
    Co-Authors: Peter K Nicholls, Sophea Heng, Ying Li, Jian Wang
    Abstract:

    Background Proprotein convertase 5/6 (PC5/6) is critical for Embryo Implantation in women, regulating both uterine epithelial receptivity and stromal cell decidualization. PC5/6 is likewise essential for Implantation in mice, but involved only in decidualization. An alternative animal model is required to address the function of PC5/6 in the uterine epithelium. This study aimed to establish whether PC5/6 is associated with Embryo Implantation in rabbits.

  • 166 Embryo Implantation is associated with specific expression of proprotein convertase 6 in the rabbit uterus
    Reproduction Fertility and Development, 2010
    Co-Authors: Ying Li, Peter K Nicholls, Sophea Heng, Jian Wang
    Abstract:

    Proprotein convertase 5/6 (PC6) is a member of the proprotein convertase family that endoproteolytically cleave latent precursor proteins into their biologically active state. We have previously demonstrated that endometrial PC6 is critical for Embryo Implantation in mice and primates, including human. PC6 regulates the endometrial physiology specifically at Implantation in association with epithelial differentiation during the establishment of endometrial receptivity (in human and monkey) and stromal cell decidualization (in the mouse, human and monkey). PC6 was further confirmed to be a unique PC member that is tightly regulated in the endometrium in relation to Implantation. Our further studies (unpublished) suggest that PC6 regulates adhesion molecules in the endometrial epithelium for Implantation in women. It is known that between the mouse and human, the endometrial stroma-mediated responses are similar whereas the epithelial cells behave differently. Because PC6 regulates primarily the stromal component (decidualization) in the mouse, in vivo mouse models are critical to investigate the roles of PC6 in decidualization. To address the function of PC6 in endometrial epithelium, non-mouse models relevant to human Implantation are required. The rabbit is regarded as an excellent model to study the molecular events of Embryo adhesion and attachment. The current study aimed to determine the expression pattern and localisation of PC6 in the rabbit uterus during early pregnancy. Quantitative RT-PCR analysis showed that PC6 mRNA expression was dynamically up-regulated in the rabbit uterus immediately prior to Implantation. Western blotting and immunohistochemical analyses demonstrated that PC6 protein was predominantly localised to the basal glands throughout pregnancy, and up-regulated specifically in the epithelium at the Embryo attachment site. These findings suggest that PC6 may play an essential role in rabbit Implantation, and that the rabbit is a useful animal model to investigate the function of PC6 during Embryo attachment.