The Experts below are selected from a list of 90 Experts worldwide ranked by ideXlab platform

Animesh Ray - One of the best experts on this subject based on the ideXlab platform.

  • short integuments1 suspensor1 carpel factory a dicer homolog is a maternal effect gene required for Embryo development in arabidopsis
    Plant Physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

  • Short Integuments1/suspensor1/carpel Factory, a Dicer Homolog, Is a Maternal Effect Gene Required for Embryo Development in Arabidopsis
    Plant physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

Teresa Golden - One of the best experts on this subject based on the ideXlab platform.

  • short integuments1 suspensor1 carpel factory a dicer homolog is a maternal effect gene required for Embryo development in arabidopsis
    Plant Physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

  • Short Integuments1/suspensor1/carpel Factory, a Dicer Homolog, Is a Maternal Effect Gene Required for Embryo Development in Arabidopsis
    Plant physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

Ueli Grossniklaus - One of the best experts on this subject based on the ideXlab platform.

  • Arabidopsis CUL3A and CUL3B genes are essential for normal Embryogenesis
    The Plant journal : for cell and molecular biology, 2005
    Co-Authors: Alexis Thomann, Vladimir Brukhin, Monika Dieterle, Jacqueline Gheyeselinck, Marylin Vantard, Ueli Grossniklaus, Pascal Genschik
    Abstract:

    Cullin (CUL)-dependent ubiquitin ligases form a class of structurally related multisubunit enzymes that control the rapid and selective degradation of important regulatory proteins involved in cell cycle progression and development, among others. The CUL3-BTB ligases belong to this class of enzymes and despite recent findings on their molecular composition, our knowledge on their functions and substrates remains still very limited. In contrast to budding and fission yeast, CUL3 is an essential gene in metazoans. The model plant Arabidopsis thaliana encodes two related CUL3 genes, called CUL3A and CUL3B. We recently reported that cul3a loss-of-function mutants are viable but exhibit a mild flowering and light sensitivity phenotype. We investigated the spatial and temporal expression of the two CUL3 genes in reproductive tissues and found that their expression Patterns are largely overlapping suggesting possible functional redundancy. Thus, we investigated the consequences on plant development of combined Arabidopsis cul3a cul3b loss-of-function mutations. Homozygous cul3b mutant plants developed normally and were fully fertile. However, the disruption of both the CUL3A and CUL3B genes reduced gametophytic transmission and caused Embryo lethality. The observed Embryo abortion was found to be under maternal control. Arrest of Embryogenesis occurred at multiple stages of Embryo development, but predominantly at the heart stage. At the cytological level, CUL3 loss-of-function mutations affected both Embryo Pattern Formation and endosperm development.

  • short integuments1 suspensor1 carpel factory a dicer homolog is a maternal effect gene required for Embryo development in arabidopsis
    Plant Physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

  • Short Integuments1/suspensor1/carpel Factory, a Dicer Homolog, Is a Maternal Effect Gene Required for Embryo Development in Arabidopsis
    Plant physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

Arcady Mushegian - One of the best experts on this subject based on the ideXlab platform.

  • short integuments1 suspensor1 carpel factory a dicer homolog is a maternal effect gene required for Embryo development in arabidopsis
    Plant Physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

  • Short Integuments1/suspensor1/carpel Factory, a Dicer Homolog, Is a Maternal Effect Gene Required for Embryo Development in Arabidopsis
    Plant physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

Stephen E Schauer - One of the best experts on this subject based on the ideXlab platform.

  • short integuments1 suspensor1 carpel factory a dicer homolog is a maternal effect gene required for Embryo development in arabidopsis
    Plant Physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.

  • Short Integuments1/suspensor1/carpel Factory, a Dicer Homolog, Is a Maternal Effect Gene Required for Embryo Development in Arabidopsis
    Plant physiology, 2002
    Co-Authors: Teresa Golden, Ueli Grossniklaus, Stephen E Schauer, Jean D Lang, Stephane Pien, Arcady Mushegian, David W Meinke, Animesh Ray
    Abstract:

    The importance of maternal cells in controlling early Embryogenesis is well understood in animal development, yet in plants the precise role of maternal cells in Embryogenesis is unclear. We demonstrated previously that maternal activity of the SIN1 (SHORT INTEGUMENTS1) gene of Arabidopsis is essential for Embryo Pattern Formation and viability, and that its postEmbryonic activity is required for several processes in reproductive development, including flowering time control and ovule morphogenesis. Here, we report the cloning of SIN1, and demonstrate its identity to the CAF (CARPEL FACTORY) gene important for normal flower morphogenesis and to the SUS1 (SUSPENSOR1) gene essential for Embryogenesis. SIN1/SUS1/CAF has sequence similarity to the Drosophila melanogaster gene Dicer, which encodes a multidomain ribonuclease specific for double-stranded RNA, first identified by its role in RNA silencing. The Dicer protein is essential for temporal control of development in animals, through the processing of small RNA hairpins that in turn inhibit the translation of target mRNAs. Structural modeling of the wild-type and sin1 mutant proteins indicates that the RNA helicase domain of SIN1/SUS1/CAF is important for function. The mRNA was detected in floral meristems, ovules, and early Embryos, consistent with the mutant phenotypes. A 3.3-kb region 5' of the SIN1/SUS1/CAF gene shows asymmetric parent-of-origin activity in the Embryo: It confers transcriptional activation of a reporter gene in early Embryos only when transmitted through the maternal gamete. These results suggest that maternal SIN1/SUS1/CAF functions early in Arabidopsis development, presumably through posttranscriptional regulation of specific mRNA molecules.