The Experts below are selected from a list of 97161 Experts worldwide ranked by ideXlab platform
Youngtae Chang - One of the best experts on this subject based on the ideXlab platform.
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development of a fluorescent chalcone library and its application in the discovery of a mouse Embryonic Stem Cell probe
Chemical Communications, 2012Co-Authors: Sungchan Lee, Namyoung Kang, Yogeswari Chandran, Youngtae Chang, Sungjin Park, Seongwook YunAbstract:We report the first fluorescent diamino-chalcone library and its application in the discovery of a mouse Embryonic Stem Cell (mESC) probe. CDg4, a novel green fluorescent mESC probe was discovered through a high-content image based screening of 160 members of the chalcone library. Interestingly, the molecular binding target of CDg4 was identified as the glycogen of the Stem Cell colony surface, rather than a conventional protein target from an intraCellular source.
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solid phase combinatorial synthesis of a xanthone library using click chemistry and its application to an Embryonic Stem Cell probe
Chemical Communications, 2011Co-Authors: Krishna Kanta Ghosh, Namyoung Kang, Yogeswari Chandran, Youngtae ChangAbstract:We report the first solid phase synthesis of a xanthone library CX and its application to Embryonic Stem Cell probe development. The CX library was further derivatised with an activated ester resin to provide an acetylated CX (CXAC) library. Screening of these libraries led to the discovery of a novel fluorescent mESC probe, CDb8.
Junghyun Kim - One of the best experts on this subject based on the ideXlab platform.
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generation of a nrf2 homozygous knockout human Embryonic Stem Cell line using crispr cas9
Stem Cell Research, 2017Co-Authors: Sojung Kim, Omer Habib, Jinsoo Kim, Hyowon Han, Soo Kyung Koo, Junghyun KimAbstract:Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian Cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human Embryonic Stem Cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 Cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.
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a homozygous keap1 knockout human Embryonic Stem Cell line generated using crispr cas9 mediates gene targeting
Stem Cell Research, 2017Co-Authors: Sojung Kim, Omer Habib, Jinsoo Kim, Hyowon Han, Soo Kyung Koo, Junghyun KimAbstract:Kelch-like ECH-associated protein 1 (keap1) is a cysteine-rich protein that interacts with transcription factor Nrf2 in a redox-sensitive manner, leading to the degradation of Nrf2 (Kim et al., 2014a). Disruption of Keap1 results in the induction of Nrf2-related signaling pathways involving the expression of a set of anti-oxidant and anti-inflammatory genes. We generated biallelic mutants of the Keap1 gene using a CRISPR-Cas9 genome editing method in the H9 human Embryonic Stem Cell (hESC). The Keap1 homozygous-knockout H9 Cell line retained normal morphology, gene expression, and in vivo differentiation potential.
Joseph H Nadeau - One of the best experts on this subject based on the ideXlab platform.
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genetic factors on mouse chromosome 18 affecting susceptibility to testicular germ Cell tumors and permissiveness to Embryonic Stem Cell derivation
Cancer Research, 2009Co-Authors: Philip D Anderson, Vicki R Nelson, Paul J Tesar, Joseph H NadeauAbstract:Despite strong heritability, little is known about the genetic control of susceptibility to testicular germ Cell tumors (TGCT) in humans or mice. Although the mouse model of spontaneous TGCTs has been extensively studied, conventional linkage analysis has failed to locate the factors that control teratocarcinogenesis in the susceptible 129 family of inbred strains. As an alternative approach, we used both chromosome substitution strains (CSS) to identify individual chromosomes that harbor susceptibility genes and a panel of congenic strains derived from a selected CSS to determine the number and location of susceptibility variants on the substituted chromosome. We showed that 129-Chr 18MOLF males are resistant to spontaneous TGCTs and that at least four genetic variants control susceptibility in males with this substituted chromosome. In addition, early Embryonic Cells from this strain fail to establish Embryonic Stem Cell lines as efficiently as those from the parental 129/Sv strain. For the first time, 129-derived genetic variants that control TGCT susceptibility and fundamental aspects of Embryonic Stem Cell biology have been localized in a genetic context in which the genes can be identified and functionally characterized. [Cancer Res 2009;69(23):9112–7]
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Genetic Factors on Mouse Chromosome 18 Affecting Susceptibility to Testicular Germ Cell Tumors and Permissiveness to Embryonic Stem Cell Derivation
Cancer Research, 2009Co-Authors: Philip D Anderson, Vicki R Nelson, Paul J Tesar, Joseph H NadeauAbstract:Despite strong heritability, little is known about the genetic control of susceptibility to testicular germ Cell tumors (TGCT) in humans or mice. Although the mouse model of spontaneous TGCTs has been extensively studied, conventional linkage analysis has failed to locate the factors that control teratocarcinogenesis in the susceptible 129 family of inbred strains. As an alternative approach, we used both chromosome substitution strains (CSS) to identify individual chromosomes that harbor susceptibility genes and a panel of congenic strains derived from a selected CSS to determine the number and location of susceptibility variants on the substituted chromosome. We showed that 129-Chr 18(MOLF) males are resistant to spontaneous TGCTs and that at least four genetic variants control susceptibility in males with this substituted chromosome. In addition, early Embryonic Cells from this strain fail to establish Embryonic Stem Cell lines as efficiently as those from the parental 129/Sv strain. For the first time, 129-derived genetic variants that control TGCT susceptibility and fundamental aspects of Embryonic Stem Cell biology have been localized in a genetic context in which the genes can be identified and functionally characterized.
Sojung Kim - One of the best experts on this subject based on the ideXlab platform.
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generation of a nrf2 homozygous knockout human Embryonic Stem Cell line using crispr cas9
Stem Cell Research, 2017Co-Authors: Sojung Kim, Omer Habib, Jinsoo Kim, Hyowon Han, Soo Kyung Koo, Junghyun KimAbstract:Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian Cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human Embryonic Stem Cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 Cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.
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a homozygous keap1 knockout human Embryonic Stem Cell line generated using crispr cas9 mediates gene targeting
Stem Cell Research, 2017Co-Authors: Sojung Kim, Omer Habib, Jinsoo Kim, Hyowon Han, Soo Kyung Koo, Junghyun KimAbstract:Kelch-like ECH-associated protein 1 (keap1) is a cysteine-rich protein that interacts with transcription factor Nrf2 in a redox-sensitive manner, leading to the degradation of Nrf2 (Kim et al., 2014a). Disruption of Keap1 results in the induction of Nrf2-related signaling pathways involving the expression of a set of anti-oxidant and anti-inflammatory genes. We generated biallelic mutants of the Keap1 gene using a CRISPR-Cas9 genome editing method in the H9 human Embryonic Stem Cell (hESC). The Keap1 homozygous-knockout H9 Cell line retained normal morphology, gene expression, and in vivo differentiation potential.
Lindy E. Barrett - One of the best experts on this subject based on the ideXlab platform.
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Generation of a TLE1 homozygous knockout human Embryonic Stem Cell line using CRISPR-Cas9.
Stem cell research, 2016Co-Authors: Amanda Herring, Kevin Eggan, Angelica Messana, Anne M. Bara, Dane Z. Hazelbaker, Lindy E. BarrettAbstract:Here, we generated a biallelic mutation in the TLE1 (Transducin Like Enhancer of Split 1) gene using CRISPR-Cas9 editing in the human Embryonic Stem Cell (hESC) line WA01. The homozygous knockout Cell line, TLE1-464-G04, displays loss of TLE1 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
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Generation of a TLE3 heterozygous knockout human Embryonic Stem Cell line using CRISPR-Cas9.
Stem cell research, 2016Co-Authors: Anne M. Bara, Kevin Eggan, Amanda Herring, Angelica Messana, Dane Z. Hazelbaker, Lindy E. BarrettAbstract:Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human Embryonic Stem Cell (hESC) line WA01. The heterozygous knockout Cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.