The Experts below are selected from a list of 23280 Experts worldwide ranked by ideXlab platform

Wai Kin Victor Chan - One of the best experts on this subject based on the ideXlab platform.

Keith A Crutcher - One of the best experts on this subject based on the ideXlab platform.

  • segregated neural explants exhibit co oriented asymmetric neurite outgrowth
    PLOS ONE, 2019
    Co-Authors: David B Pettigrew, Curtis B Dobson, Lori G Isaacson, Eric C Leuthardt, Heather N Lilley, Georgette L Suidan, Keith A Crutcher
    Abstract:

    : Explants of embryonic chick sympathetic and sensory ganglia were found to exhibit asymmetric radial outgrowth of neurites under standard culture conditions with or without exogenous Nerve Growth Factor [NGF]. Opposing sides of an explant exhibited: a) differences in neurite length and, b) differences in neurite morphology. Strikingly, this asymmetry exhibited co-orientation among segregated, neighboring explants. The underlying mechanism(s) of the asymmetry and its co-orientation are not known but appear to depend on cell clustering because dissociated sympathetic neurons do not exhibit co-orientation whereas re-aggregated clusters of cells do. This Emergent Behavior may be similar to the community effect described in other cell types. If a similar phenomenon exists in the embryo, or in maturity, it may contribute to the establishment of proper orientation of neurite outgrowth during development and/or injury-induced neuronal plasticity.

  • segregated neural explants exhibit co oriented asymmetric neurite outgrowth
    bioRxiv, 2019
    Co-Authors: David B Pettigrew, Curtis B Dobson, Lori G Isaacson, Eric C Leuthardt, Heather N Lilley, Georgette L Suidan, Keith A Crutcher
    Abstract:

    Abstract Explants of embryonic chick sympathetic and sensory ganglia were found to exhibit asymmetric radial outgrowth of neurites under standard culture conditions with or without exogenous Nerve Growth Factor [NGF]. Opposing sides of an explant exhibited: a] differences in neurite length and, b] differences in terminal morphology. Strikingly, this asymmetry exhibited co-orientation among segregated, neighboring explants. The underlying mechanism[s] of the asymmetry and its co-orientation have not been discovered but appears to depend on cell clustering because dissociated sympathetic neurons do not exhibit co-orientation whereas re-aggregated clusters of cells do. This Emergent Behavior may be similar to the community effect described in other cell types. If a similar phenomenon exists in the embryo, or in maturity, it may contribute to the establishment of proper orientation of neurite outgrowth during development and/or injury-induced neuronal plasticity.

Charles M Macal - One of the best experts on this subject based on the ideXlab platform.

David B Pettigrew - One of the best experts on this subject based on the ideXlab platform.

  • segregated neural explants exhibit co oriented asymmetric neurite outgrowth
    PLOS ONE, 2019
    Co-Authors: David B Pettigrew, Curtis B Dobson, Lori G Isaacson, Eric C Leuthardt, Heather N Lilley, Georgette L Suidan, Keith A Crutcher
    Abstract:

    : Explants of embryonic chick sympathetic and sensory ganglia were found to exhibit asymmetric radial outgrowth of neurites under standard culture conditions with or without exogenous Nerve Growth Factor [NGF]. Opposing sides of an explant exhibited: a) differences in neurite length and, b) differences in neurite morphology. Strikingly, this asymmetry exhibited co-orientation among segregated, neighboring explants. The underlying mechanism(s) of the asymmetry and its co-orientation are not known but appear to depend on cell clustering because dissociated sympathetic neurons do not exhibit co-orientation whereas re-aggregated clusters of cells do. This Emergent Behavior may be similar to the community effect described in other cell types. If a similar phenomenon exists in the embryo, or in maturity, it may contribute to the establishment of proper orientation of neurite outgrowth during development and/or injury-induced neuronal plasticity.

  • segregated neural explants exhibit co oriented asymmetric neurite outgrowth
    bioRxiv, 2019
    Co-Authors: David B Pettigrew, Curtis B Dobson, Lori G Isaacson, Eric C Leuthardt, Heather N Lilley, Georgette L Suidan, Keith A Crutcher
    Abstract:

    Abstract Explants of embryonic chick sympathetic and sensory ganglia were found to exhibit asymmetric radial outgrowth of neurites under standard culture conditions with or without exogenous Nerve Growth Factor [NGF]. Opposing sides of an explant exhibited: a] differences in neurite length and, b] differences in terminal morphology. Strikingly, this asymmetry exhibited co-orientation among segregated, neighboring explants. The underlying mechanism[s] of the asymmetry and its co-orientation have not been discovered but appears to depend on cell clustering because dissociated sympathetic neurons do not exhibit co-orientation whereas re-aggregated clusters of cells do. This Emergent Behavior may be similar to the community effect described in other cell types. If a similar phenomenon exists in the embryo, or in maturity, it may contribute to the establishment of proper orientation of neurite outgrowth during development and/or injury-induced neuronal plasticity.

De Vuyst Luc - One of the best experts on this subject based on the ideXlab platform.

  • Data from: Integrated culturing, modeling and transcriptomics uncovers complex interactions and Emergent Behavior in a three-species synthetic gut community
    2018
    Co-Authors: D'hoe Kevin, Vet Stefan, Faust Karoline, Moens Frédéric, Falony Gwen, Gonze Didier, Lloréns-rico Verónica, Gelens Lendert, Danckaert Jan, De Vuyst Luc
    Abstract:

    Whereas the composition of the human gut microbiome is well resolved, predictive understanding is still lacking. Here, we followed a bottom-up strategy to explore human gut community dynamics: we established a synthetic community composed of three representative human gut isolates (Roseburia intestinalis L1-82, Faecalibacterium prausnitzii A2-165 and Blautia hydrogenotrophica S5a33) and explored their interactions under well-controlled conditions in vitro. Systematic mono- and pair-wise fermentation experiments confirmed competition for fructose and cross-feeding of formate. We quantified with a mechanistic model how well tri-culture dynamics was predicted from mono-culture data. With the model as reference, we demonstrated that strains grown in co-culture behaved differently than in mono-culture and confirmed their altered Behavior at the transcriptional level. In addition, we showed with replicate tri-cultures and simulations that dominance in tri-culture sensitively depended on initial conditions. Our work has important implications for gut microbial community modeling as well as ecological interaction detection from batch cultures

  • Integrated culturing, modeling and transcriptomics uncovers complex interactions and Emergent Behavior in a three-species synthetic gut community
    'eLife Sciences Publications Ltd', 2018
    Co-Authors: D'hoe Kevin, Vet Stefan, Faust Karoline, Falony Gwen, Gonze Didier, Gelens Lendert, Danckaert Jan, Moens Frederic, Llorens-rico Veronica, De Vuyst Luc
    Abstract:

    The composition of the human gut microbiome is well resolved, but predictive understanding of its dynamics is still lacking. Here, we followed a bottom-up strategy to explore human gut community dynamics: we established a synthetic community composed of three representative human gut isolates (Roseburia intestinalis L1-82, Faecalibacterium prausnitzii A2-165 and Blautia hydrogenotrophica S5a33) and explored their interactions under well-controlled conditions in vitro. Systematic mono- and pair-wise fermentation experiments confirmed competition for fructose and cross-feeding of formate. We quantified with a mechanistic model how well tri-culture dynamics was predicted from mono-culture data. With the model as reference, we demonstrated that strains grown in co-culture behaved differently than those in mono-culture and confirmed their altered Behavior at the transcriptional level. In addition, we showed with replicate tri-cultures and simulations that dominance in tri-culture sensitively depends on the initial conditions. Our work has important implications for gut microbial community modeling as well as for ecological interaction detection from batch cultures.status: publishe

  • Integrated culturing, modeling and transcriptomics uncovers complex interactions and Emergent Behavior in a three-species synthetic gut community
    'eLife Sciences Publications Ltd', 2018
    Co-Authors: D’hoe Kevin, Vet Stefan, Faust Karoline, Falony Gwen, Gonze Didier, Gelens Lendert, Danckaert Jan, Moens Frederic, Llorens-rico Veronica, De Vuyst Luc
    Abstract:

    The composition of the human gut microbiome is well resolved, but predictive understanding of its dynamics is still lacking. Here, we followed a bottom-up strategy to explore human gut community dynamics: we established a synthetic community composed of three representative human gut isolates (Roseburia intestinalis L1-82, Faecalibacterium prausnitzii A2-165 and Blautia hydrogenotrophica S5a33) and explored their interactions under well-controlled conditions in vitro. Systematic mono-and pair-wise fermentation experiments confirmed competition for fructose and cross-feeding of formate. We quantified with a mechanistic model how well tri-culture dynamics was predicted from mono-culture data. With the model as reference, we demonstrated that strains grown in co-culture behaved differently than those in mono-culture and confirmed their altered Behavior at the transcriptional level. In addition, we showed with replicate tri-cultures and simulations that dominance in tri-culture sensitively depends on the initial conditions. Our work has important implications for gut microbial community modeling as well as for ecological interaction detection from batch cultures.SCOPUS: ar.jinfo:eu-repo/semantics/publishe