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Dongliang Hu - One of the best experts on this subject based on the ideXlab platform.
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the Emetic activity of staphylococcal enterotoxins sek sel sem sen and seo in a small Emetic animal model the house musk shrew
Microbiology and Immunology, 2017Co-Authors: Shouhei Hirose, Krisana Asano, Dongliang Hu, Yusuke Satoo, Ikunori Naito, Katsuhiko Omoe, Akio NakaneAbstract:: Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of Emetic food poisoning in humans. New types of SEs and SE-like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the Emetic activity of recently identified SEs using a small Emetic animal model, the house musk shrew. The Emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and Emetic responses, including the number of shrews that vomited, Emetic frequency and latency of vomiting were documented. It was found that SEs induce Emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess Emetic activity in the house musk shrew.
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the Emetic activity of staphylococcal enterotoxins sek sel sem sen and seo in a small Emetic animal model the house musk shrew
Microbiology and Immunology, 2017Co-Authors: Shouhei Hirose, Krisana Asano, Dongliang Hu, Yusuke Satoo, Ikunori Naito, Katsuhiko Omoe, Akio NakaneAbstract:: Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of Emetic food poisoning in humans. New types of SEs and SE-like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the Emetic activity of recently identified SEs using a small Emetic animal model, the house musk shrew. The Emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and Emetic responses, including the number of shrews that vomited, Emetic frequency and latency of vomiting were documented. It was found that SEs induce Emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess Emetic activity in the house musk shrew.
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induction of Emetic response to staphylococcal enterotoxins in the house musk shrew suncus murinus
Infection and Immunity, 2003Co-Authors: Yu Shimoda, Dongliang Hu, Akio Nakane, Katsuhiko Omoe, Kunihiro ShinagawaAbstract:The Emetic responses induced by staphylococcal enterotoxin A (SEA), SEB, SEC2, SED, SEE, SEG, SEH, and SEI in the house musk shrew (Suncus murinus) were investigated. SEA, SEE, and SEI showed higher Emetic activity in the house musk shrew than the other SEs. SEB, SEC2, SED, SEG, and SEH also induced Emetic responses in this animal model but relatively high doses were required. The house musk shrew appears to be a valuable model for studying the mechanisms of Emetic reactions caused by SEs.
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emesis in the shrew mouse suncus murinus induced by peroral and intraperitoneal administration of staphylococcal enterotoxin a
Journal of Food Protection, 1999Co-Authors: Dongliang Hu, Katsuhiko Omoe, Hideaki Shimura, Shunji Sugii, Kunihiro ShinagawaAbstract:Peroral and intraperitoneal administration of staphylococcal enterotoxin A (SEA) to Suncus murinus elicited an Emetic response. The 50% Emetic dose of SEA by peroral administration was found to be 32 μg per kg of body weight, whereas that by intraperitoneal administration was 3 μg per kg. Multiple Emetic responses occurred 70 to 108 min after peroral administration of an Emetic dose of SEA. Similar responses occurred 65 to 102 min after intraperitoneal injection of an Emetic dose of SEA. No significant difference in vomiting was observed between male and female animals. Anti-SEA serum neutralized SEA-induced emesis in S. murinus. These findings indicate that S. murinus may serve as a suitable animal model to study the enterotoxigenicity of SEA.
Katsuhiko Omoe - One of the best experts on this subject based on the ideXlab platform.
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the Emetic activity of staphylococcal enterotoxins sek sel sem sen and seo in a small Emetic animal model the house musk shrew
Microbiology and Immunology, 2017Co-Authors: Shouhei Hirose, Krisana Asano, Dongliang Hu, Yusuke Satoo, Ikunori Naito, Katsuhiko Omoe, Akio NakaneAbstract:: Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of Emetic food poisoning in humans. New types of SEs and SE-like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the Emetic activity of recently identified SEs using a small Emetic animal model, the house musk shrew. The Emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and Emetic responses, including the number of shrews that vomited, Emetic frequency and latency of vomiting were documented. It was found that SEs induce Emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess Emetic activity in the house musk shrew.
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the Emetic activity of staphylococcal enterotoxins sek sel sem sen and seo in a small Emetic animal model the house musk shrew
Microbiology and Immunology, 2017Co-Authors: Shouhei Hirose, Krisana Asano, Dongliang Hu, Yusuke Satoo, Ikunori Naito, Katsuhiko Omoe, Akio NakaneAbstract:: Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of Emetic food poisoning in humans. New types of SEs and SE-like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the Emetic activity of recently identified SEs using a small Emetic animal model, the house musk shrew. The Emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and Emetic responses, including the number of shrews that vomited, Emetic frequency and latency of vomiting were documented. It was found that SEs induce Emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess Emetic activity in the house musk shrew.
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induction of Emetic response to staphylococcal enterotoxins in the house musk shrew suncus murinus
Infection and Immunity, 2003Co-Authors: Yu Shimoda, Dongliang Hu, Akio Nakane, Katsuhiko Omoe, Kunihiro ShinagawaAbstract:The Emetic responses induced by staphylococcal enterotoxin A (SEA), SEB, SEC2, SED, SEE, SEG, SEH, and SEI in the house musk shrew (Suncus murinus) were investigated. SEA, SEE, and SEI showed higher Emetic activity in the house musk shrew than the other SEs. SEB, SEC2, SED, SEG, and SEH also induced Emetic responses in this animal model but relatively high doses were required. The house musk shrew appears to be a valuable model for studying the mechanisms of Emetic reactions caused by SEs.
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emesis in the shrew mouse suncus murinus induced by peroral and intraperitoneal administration of staphylococcal enterotoxin a
Journal of Food Protection, 1999Co-Authors: Dongliang Hu, Katsuhiko Omoe, Hideaki Shimura, Shunji Sugii, Kunihiro ShinagawaAbstract:Peroral and intraperitoneal administration of staphylococcal enterotoxin A (SEA) to Suncus murinus elicited an Emetic response. The 50% Emetic dose of SEA by peroral administration was found to be 32 μg per kg of body weight, whereas that by intraperitoneal administration was 3 μg per kg. Multiple Emetic responses occurred 70 to 108 min after peroral administration of an Emetic dose of SEA. Similar responses occurred 65 to 102 min after intraperitoneal injection of an Emetic dose of SEA. No significant difference in vomiting was observed between male and female animals. Anti-SEA serum neutralized SEA-induced emesis in S. murinus. These findings indicate that S. murinus may serve as a suitable animal model to study the enterotoxigenicity of SEA.
Akio Nakane - One of the best experts on this subject based on the ideXlab platform.
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the Emetic activity of staphylococcal enterotoxins sek sel sem sen and seo in a small Emetic animal model the house musk shrew
Microbiology and Immunology, 2017Co-Authors: Shouhei Hirose, Krisana Asano, Dongliang Hu, Yusuke Satoo, Ikunori Naito, Katsuhiko Omoe, Akio NakaneAbstract:: Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of Emetic food poisoning in humans. New types of SEs and SE-like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the Emetic activity of recently identified SEs using a small Emetic animal model, the house musk shrew. The Emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and Emetic responses, including the number of shrews that vomited, Emetic frequency and latency of vomiting were documented. It was found that SEs induce Emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess Emetic activity in the house musk shrew.
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the Emetic activity of staphylococcal enterotoxins sek sel sem sen and seo in a small Emetic animal model the house musk shrew
Microbiology and Immunology, 2017Co-Authors: Shouhei Hirose, Krisana Asano, Dongliang Hu, Yusuke Satoo, Ikunori Naito, Katsuhiko Omoe, Akio NakaneAbstract:: Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of Emetic food poisoning in humans. New types of SEs and SE-like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the Emetic activity of recently identified SEs using a small Emetic animal model, the house musk shrew. The Emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and Emetic responses, including the number of shrews that vomited, Emetic frequency and latency of vomiting were documented. It was found that SEs induce Emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess Emetic activity in the house musk shrew.
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induction of Emetic response to staphylococcal enterotoxins in the house musk shrew suncus murinus
Infection and Immunity, 2003Co-Authors: Yu Shimoda, Dongliang Hu, Akio Nakane, Katsuhiko Omoe, Kunihiro ShinagawaAbstract:The Emetic responses induced by staphylococcal enterotoxin A (SEA), SEB, SEC2, SED, SEE, SEG, SEH, and SEI in the house musk shrew (Suncus murinus) were investigated. SEA, SEE, and SEI showed higher Emetic activity in the house musk shrew than the other SEs. SEB, SEC2, SED, SEG, and SEH also induced Emetic responses in this animal model but relatively high doses were required. The house musk shrew appears to be a valuable model for studying the mechanisms of Emetic reactions caused by SEs.
Kunihiro Shinagawa - One of the best experts on this subject based on the ideXlab platform.
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induction of Emetic response to staphylococcal enterotoxins in the house musk shrew suncus murinus
Infection and Immunity, 2003Co-Authors: Yu Shimoda, Dongliang Hu, Akio Nakane, Katsuhiko Omoe, Kunihiro ShinagawaAbstract:The Emetic responses induced by staphylococcal enterotoxin A (SEA), SEB, SEC2, SED, SEE, SEG, SEH, and SEI in the house musk shrew (Suncus murinus) were investigated. SEA, SEE, and SEI showed higher Emetic activity in the house musk shrew than the other SEs. SEB, SEC2, SED, SEG, and SEH also induced Emetic responses in this animal model but relatively high doses were required. The house musk shrew appears to be a valuable model for studying the mechanisms of Emetic reactions caused by SEs.
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emesis in the shrew mouse suncus murinus induced by peroral and intraperitoneal administration of staphylococcal enterotoxin a
Journal of Food Protection, 1999Co-Authors: Dongliang Hu, Katsuhiko Omoe, Hideaki Shimura, Shunji Sugii, Kunihiro ShinagawaAbstract:Peroral and intraperitoneal administration of staphylococcal enterotoxin A (SEA) to Suncus murinus elicited an Emetic response. The 50% Emetic dose of SEA by peroral administration was found to be 32 μg per kg of body weight, whereas that by intraperitoneal administration was 3 μg per kg. Multiple Emetic responses occurred 70 to 108 min after peroral administration of an Emetic dose of SEA. Similar responses occurred 65 to 102 min after intraperitoneal injection of an Emetic dose of SEA. No significant difference in vomiting was observed between male and female animals. Anti-SEA serum neutralized SEA-induced emesis in S. murinus. These findings indicate that S. murinus may serve as a suitable animal model to study the enterotoxigenicity of SEA.
Paul F. Horwood - One of the best experts on this subject based on the ideXlab platform.
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Evidence for non‐ribosomal peptide synthetase production of cereulide (the Emetic toxin) in Bacillus cereus
Fems Microbiology Letters, 2004Co-Authors: Paul F. Horwood, G W Burgess, H Jane OakeyAbstract:Little is known about the process whereby the Emetic toxin (or cereulide) of Bacillus cereus is produced. Two cereulide-producing strains of B. cereus were cloned and sequenced following polymerase chain reaction (PCR) amplification with primers that were specific for conserved regions of non-ribosomal peptide synthetase (NRPS) genes. The cloned regions of the B. cereus strains were highly homologous to conserved regions of other peptide synthetase nucleotide sequences. Primers were designed for two variable regions of the NRPS gene sequence to ensure specificity for the Emetic strains. A total of 86 B. cereus strains of known Emetic or non-Emetic activity were screened using these primers. All of the Emetic strains (n=30) displayed a 188 bp band following amplification and gel electrophoresis. We have developed an improved method of identifying Emetic strains of B. cereus and provided evidence that cereulide is produced by peptide synthetases.
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evidence for non ribosomal peptide synthetase production of cereulide the Emetic toxin in bacillus cereus
Fems Microbiology Letters, 2004Co-Authors: Paul F. Horwood, G W Burgess, Jane H OakeyAbstract:Little is known about the process whereby the Emetic toxin (or cereulide) of Bacillus cereus is produced. Two cereulide-producing strains of B. cereus were cloned and sequenced following polymerase chain reaction (PCR) amplification with primers that were specific for conserved regions of non-ribosomal peptide synthetase (NRPS) genes. The cloned regions of the B. cereus strains were highly homologous to conserved regions of other peptide synthetase nucleotide sequences. Primers were designed for two variable regions of the NRPS gene sequence to ensure specificity for the Emetic strains. A total of 86 B. cereus strains of known Emetic or non-Emetic activity were screened using these primers. All of the Emetic strains (n=30) displayed a 188 bp band following amplification and gel electrophoresis. We have developed an improved method of identifying Emetic strains of B. cereus and provided evidence that cereulide is produced by peptide synthetases.