The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
J W Vaughan - One of the best experts on this subject based on the ideXlab platform.
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evaluation of materials used for bedding Encasement effect of pore size in blocking cat and dust mite allergen
The Journal of Allergy and Clinical Immunology, 1999Co-Authors: J W Vaughan, T. E. Mclaughlin, Matthew S. Perzanowski, Thomas A E PlattsmillsAbstract:Abstract Background: Mattress and pillow encasings are recommended for patients allergic to dust mites. Many Encasements block allergen and are vapor permeable but do not allow free passage of air through the material. Recently, breathable fabrics made from tightly woven synthetic fibers or nonwoven synthetics have been recommend as Encasements. Objective: The purpose of this study was to develop a method for testing Encasement materials made of breathable fabrics. Methods: Dust samples containing a known quantity of allergen (Der f 1, Der p 1, and Fel d 1) were pulled across a variety of fabrics using a modified dust trap. Airflow through the dust trap was controlled with a vacuum pump. Five minutes after dust was introduced, the pump was shut off. A filter located downstream of the fabric collected allergen passing through the fabric during the test and was assayed with ELISA for the relevant allergen. Fabrics to be tested were obtained from manufacturers and specialty catalogs. Results: As the average pore size decreases, the airflow through a fabric becomes restricted, and the pressure differential created by the vacuum pump increases. Dust mite allergens (Der f 1 and Der p 1) were blocked below detectable limits by fabrics of less than 10 μm in pore size. Fabrics with an average pore size of 6 μm or less blocked cat allergen (Fel d 1). Conclusion: The method we developed provided a rigorous and reliable test for leakage of common indoor allergens through breathable barrier fabrics. Our results show that tightly woven fabrics and nonwoven synthetic fabrics can block common indoor allergens but still allow airflow. (J Allergy Clin Immunol 1999;103:227-31.)
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Evaluation of materials used for bedding Encasement: effect of pore size in blocking cat and dust mite allergen.
The Journal of allergy and clinical immunology, 1999Co-Authors: J W Vaughan, T. E. Mclaughlin, Matthew S. Perzanowski, Thomas A.e. Platts-millsAbstract:BACKGROUND: Mattress and pillow encasings are recommended for patients allergic to dust mites. Many Encasements block allergen and are vapor permeable but do not allow free passage of air through the material. Recently, breathable fabrics made from tightly woven synthetic fibers or nonwoven synthetics have been recommend as Encasements. OBJECTIVE: The purpose of this study was to develop a method for testing Encasement materials made of breathable fabrics. METHODS: Dust samples containing a known quantity of allergen (Der f 1, Der p 1, and Fel d 1) were pulled across a variety of fabrics using a modified dust trap. Airflow through the dust trap was controlled with a vacuum pump. Five minutes after dust was introduced, the pump was shut off. A filter located downstream of the fabric collected allergen passing through the fabric during the test and was assayed with ELISA for the relevant allergen. Fabrics to be tested were obtained from manufacturers and specialty catalogs. RESULTS: As the average pore size decreases, the airflow through a fabric becomes restricted, and the pressure differential created by the vacuum pump increases. Dust mite allergens (Der f 1 and Der p 1) were blocked below detectable limits by fabrics of less than 10 microm in pore size. Fabrics with an average pore size of 6 microm or less blocked cat allergen (Fel d 1). CONCLUSION: The method we developed provided a rigorous and reliable test for leakage of common indoor allergens through breathable barrier fabrics. Our results show that tightly woven fabrics and nonwoven synthetic fabrics can block common indoor allergens but still allow airflow.
John A Chabot - One of the best experts on this subject based on the ideXlab platform.
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resection of locally advanced pancreatic cancer without regression of arterial Encasement after modern era neoadjuvant therapy
Journal of Gastrointestinal Surgery, 2018Co-Authors: Michael D Kluger, Farzan M Rashid, Vilma Rosario, Beth Schrope, Jonathan Steinman, Elizabeth M Hecht, John A ChabotAbstract:Introduction Modern-era systemic therapy for locally advanced pancreatic adenocarcinoma (LAPC) offers improved survival relative to historical regimens but not necessarily improved radiographic downstaging to allow more patients to undergo resection. The aim of this study was to evaluate the survival, progression, and pathologic outcomes after resection of LAPC that did not regress from > 180 degrees arterial Encasement after neoadjuvant therapy.
Patrick Eichenberger - One of the best experts on this subject based on the ideXlab platform.
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Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization
Journal of Physics D: Applied Physics, 2016Co-Authors: Marina Raguse, Marcel Fiebrandt, Katharina Stapelmann, Adam Driks, Peter Eaton, Benjamin Denis, Patrick Eichenberger, Peter Awakowicz, Ralf MoellerAbstract:© 2016 IOP Publishing Ltd. Low-pressure plasmas have been evaluated for their potential in biomedical and defense purposes. The sterilizing effect of plasma can be attributed to several active agents, including (V)UV radiation, charged particles, radical species, neutral and excited atoms and molecules, and the electric field. Spores of Bacillus subtilis were used as a bioindicator and a genetic model system to study the sporicidal effects of low-pressure plasma decontamination. Wild-type spores, spores lacking the major protective coat layers (inner, outer, and crust), pigmentation-deficient spores or spore impaired in Encasement (a late step in coat assembly) were systematically tested for their resistance to low-pressure argon, hydrogen, and oxygen plasmas with and without admixtures. We demonstrate that low-pressure plasma discharges of argon and oxygen discharges cause significant physical damage to spore surface structures as visualized by atomic force microscopy. Spore resistance to low-pressure plasma was primarily dependent on the presence of the inner, and outer spore coat layers as well as spore Encasement, with minor or less importance of the crust and spore pigmentation, whereas spore inactivation itself was strongly influenced by the gas composition and operational settings.
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physical interaction between coat morphogenetic proteins spovid and cote is necessary for spore Encasement in bacillus subtilis
Journal of Bacteriology, 2012Co-Authors: Melissa D De Francesco, Peter T Mckenney, Ming Hsiu Chua, Jake Z Jacobs, Filipa Nunes, Monica Serrano, Adriano O Henriques, Patrick EichenbergerAbstract:Endospore formation by Bacillus subtilis is a complex and dynamic process. One of the major challenges of sporulation is the assembly of a protective, multilayered, proteinaceous spore coat, composed of at least 70 different proteins. Spore coat formation can be divided into two distinct stages. The first is the recruitment of proteins to the spore surface, dependent on the morphogenetic protein SpoIVA. The second step, known as Encasement, involves the migration of the coat proteins around the circumference of the spore in successive waves, a process dependent on the morphogenetic protein SpoVID and the transcriptional regulation of individual coat genes. We provide genetic and biochemical evidence supporting the hypothesis that SpoVID promotes Encasement of the spore by establishing direct protein-protein interactions with other coat morphogenetic proteins. It was previously demonstrated that SpoVID directly interacts with SpoIVA and the inner coat morphogenetic protein, SafA. Here, we show by yeast two-hybrid and pulldown assays that SpoVID also interacts directly with the outer coat morphogenetic protein, CotE. Furthermore, by mutational analysis, we identified a specific residue in the N-terminal domain of SpoVID that is essential for the interaction with CotE but dispensable for the interaction with SafA. We propose an updated model of coat assembly and spore Encasement that incorporates several physical interactions between the principal coat morphogenetic proteins.
Thomas A E Plattsmills - One of the best experts on this subject based on the ideXlab platform.
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evaluation of materials used for bedding Encasement effect of pore size in blocking cat and dust mite allergen
The Journal of Allergy and Clinical Immunology, 1999Co-Authors: J W Vaughan, T. E. Mclaughlin, Matthew S. Perzanowski, Thomas A E PlattsmillsAbstract:Abstract Background: Mattress and pillow encasings are recommended for patients allergic to dust mites. Many Encasements block allergen and are vapor permeable but do not allow free passage of air through the material. Recently, breathable fabrics made from tightly woven synthetic fibers or nonwoven synthetics have been recommend as Encasements. Objective: The purpose of this study was to develop a method for testing Encasement materials made of breathable fabrics. Methods: Dust samples containing a known quantity of allergen (Der f 1, Der p 1, and Fel d 1) were pulled across a variety of fabrics using a modified dust trap. Airflow through the dust trap was controlled with a vacuum pump. Five minutes after dust was introduced, the pump was shut off. A filter located downstream of the fabric collected allergen passing through the fabric during the test and was assayed with ELISA for the relevant allergen. Fabrics to be tested were obtained from manufacturers and specialty catalogs. Results: As the average pore size decreases, the airflow through a fabric becomes restricted, and the pressure differential created by the vacuum pump increases. Dust mite allergens (Der f 1 and Der p 1) were blocked below detectable limits by fabrics of less than 10 μm in pore size. Fabrics with an average pore size of 6 μm or less blocked cat allergen (Fel d 1). Conclusion: The method we developed provided a rigorous and reliable test for leakage of common indoor allergens through breathable barrier fabrics. Our results show that tightly woven fabrics and nonwoven synthetic fabrics can block common indoor allergens but still allow airflow. (J Allergy Clin Immunol 1999;103:227-31.)
Thomas A.e. Platts-mills - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of materials used for bedding Encasement: effect of pore size in blocking cat and dust mite allergen.
The Journal of allergy and clinical immunology, 1999Co-Authors: J W Vaughan, T. E. Mclaughlin, Matthew S. Perzanowski, Thomas A.e. Platts-millsAbstract:BACKGROUND: Mattress and pillow encasings are recommended for patients allergic to dust mites. Many Encasements block allergen and are vapor permeable but do not allow free passage of air through the material. Recently, breathable fabrics made from tightly woven synthetic fibers or nonwoven synthetics have been recommend as Encasements. OBJECTIVE: The purpose of this study was to develop a method for testing Encasement materials made of breathable fabrics. METHODS: Dust samples containing a known quantity of allergen (Der f 1, Der p 1, and Fel d 1) were pulled across a variety of fabrics using a modified dust trap. Airflow through the dust trap was controlled with a vacuum pump. Five minutes after dust was introduced, the pump was shut off. A filter located downstream of the fabric collected allergen passing through the fabric during the test and was assayed with ELISA for the relevant allergen. Fabrics to be tested were obtained from manufacturers and specialty catalogs. RESULTS: As the average pore size decreases, the airflow through a fabric becomes restricted, and the pressure differential created by the vacuum pump increases. Dust mite allergens (Der f 1 and Der p 1) were blocked below detectable limits by fabrics of less than 10 microm in pore size. Fabrics with an average pore size of 6 microm or less blocked cat allergen (Fel d 1). CONCLUSION: The method we developed provided a rigorous and reliable test for leakage of common indoor allergens through breathable barrier fabrics. Our results show that tightly woven fabrics and nonwoven synthetic fabrics can block common indoor allergens but still allow airflow.