The Experts below are selected from a list of 129 Experts worldwide ranked by ideXlab platform

Ann C. Palmenberg - One of the best experts on this subject based on the ideXlab platform.

  • Deletion Mapping of the Encephalomyocarditis Virus Primary Cleavage Site
    Journal of Virology, 2001
    Co-Authors: Harry Hahn, Ann C. Palmenberg
    Abstract:

    : The cotranslational, primary self-cleavage reaction of cardioVirus polyprotein relies on a highly conserved, short segment of amino acids at the 2A-2B protein boundary. The amino terminus of the required element for Encephalomyocarditis Virus has now been mapped to include Tyr(126) of the 2A protein, the 18th amino acid before the cleavage site.

  • Mutational analysis of the Encephalomyocarditis Virus primary cleavage.
    Journal of virology, 1996
    Co-Authors: Harry Hahn, Ann C. Palmenberg
    Abstract:

    Sixteen substitution mutations of the conserved DvExNPGP sequence, implicated in cardioVirus and aphthoVirus primary polyprotein cleavage, were created in Encephalomyocarditis Virus cDNA, expressed, and characterized for processing activity. Nearly all the mutations severely decreased the efficiency of the primary cleavage reaction during cell-free synthesis of viral precursors, indicating a stringent requirement for the natural sequence in this processing event. When representative mutations were tested in full-length genomic contexts, they were lethal and no revertants were observed. Not only were the primary cleavage reactions deficient in these polyproteins, but subsequent cleavage of P1 by endogenous or exogenous 3C pro was also impaired. This indicates that primary cleavage has a role in the proper processing of the viral capsid precursor.

Harry Hahn - One of the best experts on this subject based on the ideXlab platform.

  • Deletion Mapping of the Encephalomyocarditis Virus Primary Cleavage Site
    Journal of Virology, 2001
    Co-Authors: Harry Hahn, Ann C. Palmenberg
    Abstract:

    : The cotranslational, primary self-cleavage reaction of cardioVirus polyprotein relies on a highly conserved, short segment of amino acids at the 2A-2B protein boundary. The amino terminus of the required element for Encephalomyocarditis Virus has now been mapped to include Tyr(126) of the 2A protein, the 18th amino acid before the cleavage site.

  • Mutational analysis of the Encephalomyocarditis Virus primary cleavage.
    Journal of virology, 1996
    Co-Authors: Harry Hahn, Ann C. Palmenberg
    Abstract:

    Sixteen substitution mutations of the conserved DvExNPGP sequence, implicated in cardioVirus and aphthoVirus primary polyprotein cleavage, were created in Encephalomyocarditis Virus cDNA, expressed, and characterized for processing activity. Nearly all the mutations severely decreased the efficiency of the primary cleavage reaction during cell-free synthesis of viral precursors, indicating a stringent requirement for the natural sequence in this processing event. When representative mutations were tested in full-length genomic contexts, they were lethal and no revertants were observed. Not only were the primary cleavage reactions deficient in these polyproteins, but subsequent cleavage of P1 by endogenous or exogenous 3C pro was also impaired. This indicates that primary cleavage has a role in the proper processing of the viral capsid precursor.

Vadim I. Agol - One of the best experts on this subject based on the ideXlab platform.

  • Encephalomyocarditis Virus RNA polymerase preparations, with and without RNA helicase activity.
    Journal of virology, 1991
    Co-Authors: T.m. Dmitrieva, K B Norkina, Vadim I. Agol
    Abstract:

    RNA template- and primer-dependent preparations of RNA polymerase were purified from Encephalomyocarditis Virus-infected Krebs-2 cells, using a three-step chromatographic procedure. The RNA duplex-unwinding activity of these preparations was investigated by two assays, using a partially double-stranded RNA template (Encephalomyocarditis Virus RNA annealed with a long segment of antisense transcript). Less purified preparations of the polymerase appeared to be able to efficiently displace, in an ATP-dependent and RNA elongation-dependent reaction, the antisense segment from the template. However, upon a more extensive purification, the unwinding activity of the RNA polymerase preparations was lost.

Nahum Sonenberg - One of the best experts on this subject based on the ideXlab platform.

  • Cell-Free Synthesis of Encephalomyocarditis Virus
    Journal of virology, 2003
    Co-Authors: Yuri V. Svitkin, Nahum Sonenberg
    Abstract:

    We developed a system for complete replication of Encephalomyocarditis Virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient Virus synthesis occurred in a narrow range of Mg2+ and EMCV RNA concentrations. Excess input RNA impaired RNA replication and Virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.

Mitsuru Seishima - One of the best experts on this subject based on the ideXlab platform.