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Ludwik K Malendowicz - One of the best experts on this subject based on the ideXlab platform.
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expression of neuromedins s and u and their receptors in the hypothalamus and Endocrine Glands of the rat
International Journal of Molecular Medicine, 2007Co-Authors: Marcin Rucinski, Agnieszka Ziolkowska, Giuliano Neri, Marcin Trejter, Tomasz Zemleduch, Marianna Tyczewska, Gastone G Nussdorfer, Ludwik K MalendowiczAbstract:Neuromedin S (NMS) and neuromedin U (NMU) are regulatory peptides that share the C-terminal amino-acid sequence and act via common G protein-coupled receptors called NMUR1 and NMUR2. Semiquantitative real time-PCR showed that in the rat hypothalamus and testis NMS gene expression was markedly higher than that of the NMU gene, while the reverse occurred in the anterior pituitary and thyroid gland. Low expression of both genes was detected in the thymus, adrenal gland and ovary, whereas in the pancreatic islets only the expression of NMU mRNA was detected. In the rat hypothalamus the expression of the NMUR2 gene was strikingly higher than that of the NMUR1 gene; in contrast, in the testis and ovary the very low expression of NMUR2 contrasted with the relatively high expression of the NMUR1 gene. In the other Glands examined only expression of the NMUR1 gene was found. The marked differences in the level of expression of NMU, NMS and their receptors in the hypothalamus and Endocrine Glands of the rat suggest that in this species such neuromedins may play different roles in the functional regulation of neuroEndocrine axes.
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Expression of neuropeptides B and W and their receptors in Endocrine Glands of the rat
International journal of molecular medicine, 2006Co-Authors: Anna Hochol, Marcin Rucinski, Agnieszka Ziolkowska, Gastone G Nussdorfer, Rosa Di Liddo, Anna S. Belloni, Ludwik K MalendowiczAbstract:Neuropeptides B and W (NPB and NPW) have been identified as endogenous ligands of the G protein-coupled receptors (GPR) 7 and 8, which in humans are expressed in the hypothalamus and probably involved in the regulation of energy homeostasis and feeding behavior. GPR8 is absent in the rat, where the GPR8-like receptor (GPR8-LR) has been described. Reverse transcription-polymerase chain reaction detected the expression of NPB, NPW, GPR7 and GPR8-LR mRNAs in the hypothalamus, anterior pituitary, thyroid and parathyroid Glands, pancreatic islets, adrenal Glands, ovary and testis of the rat. Immunocytochemistry demonstrated the presence of NPB and NPW immunoreactivities in these same Glands. Radioimmune assay showed that the bolus intraperitoneal injection of 2 nmol/100 g NPB or NPW raised the plasma levels of parathyroid hormone, corticosterone and testosterone. NPB also increased the blood concentration of thyroxine, and NPW that of ACTH and estradiol. Taken together, these findings allow us to suggest that NPB and NPW play a role in the autocrine-paracrine functional regulation of the Endocrine system in the rat.
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role of tachykinin gene related peptides in the local regulation of Endocrine Glands
Handbook of Biologically Active Peptides, 2006Co-Authors: Ludwik K MalendowiczAbstract:ABSTRACT The mammalian tachykinins are a family of peptides that act as neurotransmitters, neuromodulators, and probably as hormones. They are encoded by three genes, TAC 1, TAC 3, and TAC 4. Tachykinin-gene-related peptides (TGRP), which include substance P (SP), neurokinin A (NKA), and neurokinin B (NKB), exert their biological actions through three G-protein-coupled receptors, named NK1, NK2, and NK3. Expression of preprotachykinin genes and TGRP receptors, TGRP localization, as well as their direct effects on the Endocrine Glands (pituitary, thyroid gland, parathyroid, pancreatic islets, adrenal gland, Leydig cells, and ovary) are surveyed in this chapter.
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expression of the beacon gene in Endocrine Glands of the rat
Peptides, 2004Co-Authors: Agnieszka Ziolkowska, Marcin Rucinski, Gastone G Nussdorfer, Rosa Di Liddo, Ludwik K MalendowiczAbstract:Abstract Beacon gene has been recently identified in the rat hypothalamus, and reported to be overexpressed in obese animals. This pattern of expression suggests that beacon may be involved in the functional regulation of neuroEndocrine axes. Hence, we have investigated the expression of beacon in the Endocrine system of the rat. Reverse transcription–polymerase chain reaction showed the expression of beacon mRNA in the hypothalamus, adenohypophysis, thyroid gland, adrenal gland, testis, ovary and pancreatic islets. Immunocytochemistry demonstrated the presence of the beacon immunoreactivity in all tissues studied, the staining being very intense in the neurons of paraventricular and supraoptic nuclei, the basophils of adenohypophysis, the parathyroid gland, adrenocortical cells, testis Leydig cells, ovary thecal, granulosa and lutein cells, and pancreatic islets. Due the fact that beacon has been included in the ubiquitin-like protein family, its widespread expression in rat Endocrine tissues is not astonishing. The in vivo administration of beacon[47–73] (3.5 nmol/100 body weight) elicited within 60 min a marked decrease in the plasma concentration of ACTH, aldosterone and corticosterone, and a moderate lowering of the blood levels of testosterone and estradiol. This finding suggests that beacon exerts a negative modulatory action on the pituitary–adrenal axis and gonad secretory activity, whose physiological relevance remains, however, to be established.
Marcin Rucinski - One of the best experts on this subject based on the ideXlab platform.
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expression of neuromedins s and u and their receptors in the hypothalamus and Endocrine Glands of the rat
International Journal of Molecular Medicine, 2007Co-Authors: Marcin Rucinski, Agnieszka Ziolkowska, Giuliano Neri, Marcin Trejter, Tomasz Zemleduch, Marianna Tyczewska, Gastone G Nussdorfer, Ludwik K MalendowiczAbstract:Neuromedin S (NMS) and neuromedin U (NMU) are regulatory peptides that share the C-terminal amino-acid sequence and act via common G protein-coupled receptors called NMUR1 and NMUR2. Semiquantitative real time-PCR showed that in the rat hypothalamus and testis NMS gene expression was markedly higher than that of the NMU gene, while the reverse occurred in the anterior pituitary and thyroid gland. Low expression of both genes was detected in the thymus, adrenal gland and ovary, whereas in the pancreatic islets only the expression of NMU mRNA was detected. In the rat hypothalamus the expression of the NMUR2 gene was strikingly higher than that of the NMUR1 gene; in contrast, in the testis and ovary the very low expression of NMUR2 contrasted with the relatively high expression of the NMUR1 gene. In the other Glands examined only expression of the NMUR1 gene was found. The marked differences in the level of expression of NMU, NMS and their receptors in the hypothalamus and Endocrine Glands of the rat suggest that in this species such neuromedins may play different roles in the functional regulation of neuroEndocrine axes.
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Expression of neuropeptides B and W and their receptors in Endocrine Glands of the rat
International journal of molecular medicine, 2006Co-Authors: Anna Hochol, Marcin Rucinski, Agnieszka Ziolkowska, Gastone G Nussdorfer, Rosa Di Liddo, Anna S. Belloni, Ludwik K MalendowiczAbstract:Neuropeptides B and W (NPB and NPW) have been identified as endogenous ligands of the G protein-coupled receptors (GPR) 7 and 8, which in humans are expressed in the hypothalamus and probably involved in the regulation of energy homeostasis and feeding behavior. GPR8 is absent in the rat, where the GPR8-like receptor (GPR8-LR) has been described. Reverse transcription-polymerase chain reaction detected the expression of NPB, NPW, GPR7 and GPR8-LR mRNAs in the hypothalamus, anterior pituitary, thyroid and parathyroid Glands, pancreatic islets, adrenal Glands, ovary and testis of the rat. Immunocytochemistry demonstrated the presence of NPB and NPW immunoreactivities in these same Glands. Radioimmune assay showed that the bolus intraperitoneal injection of 2 nmol/100 g NPB or NPW raised the plasma levels of parathyroid hormone, corticosterone and testosterone. NPB also increased the blood concentration of thyroxine, and NPW that of ACTH and estradiol. Taken together, these findings allow us to suggest that NPB and NPW play a role in the autocrine-paracrine functional regulation of the Endocrine system in the rat.
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expression of the beacon gene in Endocrine Glands of the rat
Peptides, 2004Co-Authors: Agnieszka Ziolkowska, Marcin Rucinski, Gastone G Nussdorfer, Rosa Di Liddo, Ludwik K MalendowiczAbstract:Abstract Beacon gene has been recently identified in the rat hypothalamus, and reported to be overexpressed in obese animals. This pattern of expression suggests that beacon may be involved in the functional regulation of neuroEndocrine axes. Hence, we have investigated the expression of beacon in the Endocrine system of the rat. Reverse transcription–polymerase chain reaction showed the expression of beacon mRNA in the hypothalamus, adenohypophysis, thyroid gland, adrenal gland, testis, ovary and pancreatic islets. Immunocytochemistry demonstrated the presence of the beacon immunoreactivity in all tissues studied, the staining being very intense in the neurons of paraventricular and supraoptic nuclei, the basophils of adenohypophysis, the parathyroid gland, adrenocortical cells, testis Leydig cells, ovary thecal, granulosa and lutein cells, and pancreatic islets. Due the fact that beacon has been included in the ubiquitin-like protein family, its widespread expression in rat Endocrine tissues is not astonishing. The in vivo administration of beacon[47–73] (3.5 nmol/100 body weight) elicited within 60 min a marked decrease in the plasma concentration of ACTH, aldosterone and corticosterone, and a moderate lowering of the blood levels of testosterone and estradiol. This finding suggests that beacon exerts a negative modulatory action on the pituitary–adrenal axis and gonad secretory activity, whose physiological relevance remains, however, to be established.
Agnieszka Ziolkowska - One of the best experts on this subject based on the ideXlab platform.
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expression of neuromedins s and u and their receptors in the hypothalamus and Endocrine Glands of the rat
International Journal of Molecular Medicine, 2007Co-Authors: Marcin Rucinski, Agnieszka Ziolkowska, Giuliano Neri, Marcin Trejter, Tomasz Zemleduch, Marianna Tyczewska, Gastone G Nussdorfer, Ludwik K MalendowiczAbstract:Neuromedin S (NMS) and neuromedin U (NMU) are regulatory peptides that share the C-terminal amino-acid sequence and act via common G protein-coupled receptors called NMUR1 and NMUR2. Semiquantitative real time-PCR showed that in the rat hypothalamus and testis NMS gene expression was markedly higher than that of the NMU gene, while the reverse occurred in the anterior pituitary and thyroid gland. Low expression of both genes was detected in the thymus, adrenal gland and ovary, whereas in the pancreatic islets only the expression of NMU mRNA was detected. In the rat hypothalamus the expression of the NMUR2 gene was strikingly higher than that of the NMUR1 gene; in contrast, in the testis and ovary the very low expression of NMUR2 contrasted with the relatively high expression of the NMUR1 gene. In the other Glands examined only expression of the NMUR1 gene was found. The marked differences in the level of expression of NMU, NMS and their receptors in the hypothalamus and Endocrine Glands of the rat suggest that in this species such neuromedins may play different roles in the functional regulation of neuroEndocrine axes.
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Expression of neuropeptides B and W and their receptors in Endocrine Glands of the rat
International journal of molecular medicine, 2006Co-Authors: Anna Hochol, Marcin Rucinski, Agnieszka Ziolkowska, Gastone G Nussdorfer, Rosa Di Liddo, Anna S. Belloni, Ludwik K MalendowiczAbstract:Neuropeptides B and W (NPB and NPW) have been identified as endogenous ligands of the G protein-coupled receptors (GPR) 7 and 8, which in humans are expressed in the hypothalamus and probably involved in the regulation of energy homeostasis and feeding behavior. GPR8 is absent in the rat, where the GPR8-like receptor (GPR8-LR) has been described. Reverse transcription-polymerase chain reaction detected the expression of NPB, NPW, GPR7 and GPR8-LR mRNAs in the hypothalamus, anterior pituitary, thyroid and parathyroid Glands, pancreatic islets, adrenal Glands, ovary and testis of the rat. Immunocytochemistry demonstrated the presence of NPB and NPW immunoreactivities in these same Glands. Radioimmune assay showed that the bolus intraperitoneal injection of 2 nmol/100 g NPB or NPW raised the plasma levels of parathyroid hormone, corticosterone and testosterone. NPB also increased the blood concentration of thyroxine, and NPW that of ACTH and estradiol. Taken together, these findings allow us to suggest that NPB and NPW play a role in the autocrine-paracrine functional regulation of the Endocrine system in the rat.
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expression of the beacon gene in Endocrine Glands of the rat
Peptides, 2004Co-Authors: Agnieszka Ziolkowska, Marcin Rucinski, Gastone G Nussdorfer, Rosa Di Liddo, Ludwik K MalendowiczAbstract:Abstract Beacon gene has been recently identified in the rat hypothalamus, and reported to be overexpressed in obese animals. This pattern of expression suggests that beacon may be involved in the functional regulation of neuroEndocrine axes. Hence, we have investigated the expression of beacon in the Endocrine system of the rat. Reverse transcription–polymerase chain reaction showed the expression of beacon mRNA in the hypothalamus, adenohypophysis, thyroid gland, adrenal gland, testis, ovary and pancreatic islets. Immunocytochemistry demonstrated the presence of the beacon immunoreactivity in all tissues studied, the staining being very intense in the neurons of paraventricular and supraoptic nuclei, the basophils of adenohypophysis, the parathyroid gland, adrenocortical cells, testis Leydig cells, ovary thecal, granulosa and lutein cells, and pancreatic islets. Due the fact that beacon has been included in the ubiquitin-like protein family, its widespread expression in rat Endocrine tissues is not astonishing. The in vivo administration of beacon[47–73] (3.5 nmol/100 body weight) elicited within 60 min a marked decrease in the plasma concentration of ACTH, aldosterone and corticosterone, and a moderate lowering of the blood levels of testosterone and estradiol. This finding suggests that beacon exerts a negative modulatory action on the pituitary–adrenal axis and gonad secretory activity, whose physiological relevance remains, however, to be established.
Gastone G Nussdorfer - One of the best experts on this subject based on the ideXlab platform.
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expression of neuromedins s and u and their receptors in the hypothalamus and Endocrine Glands of the rat
International Journal of Molecular Medicine, 2007Co-Authors: Marcin Rucinski, Agnieszka Ziolkowska, Giuliano Neri, Marcin Trejter, Tomasz Zemleduch, Marianna Tyczewska, Gastone G Nussdorfer, Ludwik K MalendowiczAbstract:Neuromedin S (NMS) and neuromedin U (NMU) are regulatory peptides that share the C-terminal amino-acid sequence and act via common G protein-coupled receptors called NMUR1 and NMUR2. Semiquantitative real time-PCR showed that in the rat hypothalamus and testis NMS gene expression was markedly higher than that of the NMU gene, while the reverse occurred in the anterior pituitary and thyroid gland. Low expression of both genes was detected in the thymus, adrenal gland and ovary, whereas in the pancreatic islets only the expression of NMU mRNA was detected. In the rat hypothalamus the expression of the NMUR2 gene was strikingly higher than that of the NMUR1 gene; in contrast, in the testis and ovary the very low expression of NMUR2 contrasted with the relatively high expression of the NMUR1 gene. In the other Glands examined only expression of the NMUR1 gene was found. The marked differences in the level of expression of NMU, NMS and their receptors in the hypothalamus and Endocrine Glands of the rat suggest that in this species such neuromedins may play different roles in the functional regulation of neuroEndocrine axes.
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Expression of neuropeptides B and W and their receptors in Endocrine Glands of the rat
International journal of molecular medicine, 2006Co-Authors: Anna Hochol, Marcin Rucinski, Agnieszka Ziolkowska, Gastone G Nussdorfer, Rosa Di Liddo, Anna S. Belloni, Ludwik K MalendowiczAbstract:Neuropeptides B and W (NPB and NPW) have been identified as endogenous ligands of the G protein-coupled receptors (GPR) 7 and 8, which in humans are expressed in the hypothalamus and probably involved in the regulation of energy homeostasis and feeding behavior. GPR8 is absent in the rat, where the GPR8-like receptor (GPR8-LR) has been described. Reverse transcription-polymerase chain reaction detected the expression of NPB, NPW, GPR7 and GPR8-LR mRNAs in the hypothalamus, anterior pituitary, thyroid and parathyroid Glands, pancreatic islets, adrenal Glands, ovary and testis of the rat. Immunocytochemistry demonstrated the presence of NPB and NPW immunoreactivities in these same Glands. Radioimmune assay showed that the bolus intraperitoneal injection of 2 nmol/100 g NPB or NPW raised the plasma levels of parathyroid hormone, corticosterone and testosterone. NPB also increased the blood concentration of thyroxine, and NPW that of ACTH and estradiol. Taken together, these findings allow us to suggest that NPB and NPW play a role in the autocrine-paracrine functional regulation of the Endocrine system in the rat.
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expression of the beacon gene in Endocrine Glands of the rat
Peptides, 2004Co-Authors: Agnieszka Ziolkowska, Marcin Rucinski, Gastone G Nussdorfer, Rosa Di Liddo, Ludwik K MalendowiczAbstract:Abstract Beacon gene has been recently identified in the rat hypothalamus, and reported to be overexpressed in obese animals. This pattern of expression suggests that beacon may be involved in the functional regulation of neuroEndocrine axes. Hence, we have investigated the expression of beacon in the Endocrine system of the rat. Reverse transcription–polymerase chain reaction showed the expression of beacon mRNA in the hypothalamus, adenohypophysis, thyroid gland, adrenal gland, testis, ovary and pancreatic islets. Immunocytochemistry demonstrated the presence of the beacon immunoreactivity in all tissues studied, the staining being very intense in the neurons of paraventricular and supraoptic nuclei, the basophils of adenohypophysis, the parathyroid gland, adrenocortical cells, testis Leydig cells, ovary thecal, granulosa and lutein cells, and pancreatic islets. Due the fact that beacon has been included in the ubiquitin-like protein family, its widespread expression in rat Endocrine tissues is not astonishing. The in vivo administration of beacon[47–73] (3.5 nmol/100 body weight) elicited within 60 min a marked decrease in the plasma concentration of ACTH, aldosterone and corticosterone, and a moderate lowering of the blood levels of testosterone and estradiol. This finding suggests that beacon exerts a negative modulatory action on the pituitary–adrenal axis and gonad secretory activity, whose physiological relevance remains, however, to be established.
Hua Wang - One of the best experts on this subject based on the ideXlab platform.
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Endocrine Glands derived vascular endothelial growth factor protects pancreatic cancer cells from apoptosis via upregulation of the myeloid cell leukemia 1 protein
Biochemical and Biophysical Research Communications, 2009Co-Authors: Linan Ren, Fengjun Xiao, Jun Yan, Yuefeng Yang, Lisheng Wang, Xiaozhong Guo, Hua WangAbstract:Abstract Endocrine Glands-derived vascular endothelial growth factor (EG-VEGF, also termed as Prok1)—a novel cytokine that selectively acts on the endothelial cells of Endocrine Glands—was recently reported to be involved in the regulation of tumor cell growth and survival. However, its roles in the regulation of pancreatic cancer progression remain unclear. In this report, we investigated the suppressive effects of EG-VEGF on pancreatic cancer cell apoptosis and the relevant mechanisms. By using reverse-transcriptase polymerase chain reaction (RT-PCR) we found that the Mia PaCa II cells of the pancreatic cancer cell line express the mRNAs of both EG-VEGF (Prok1) and its receptors. EG-VEGF protects pancreatic cancer cells from apoptosis through upregulation of myeloid cell leukemia-1 (Mcl-1), an anti-apoptotic protein of the bcl-2 family. Treatment of pancreatic cancer cells with EG-VEGF results in the rapid phosphorylation of mitogen-activated protein kinase (MAPK), STAT3, and AKT, which are involved in the upregulation of Mcl-1 expression. EG-VEGF (Prok1) protects Mia PaCa II cells from apoptosis through G protein-coupled receptor (GPR)-induced activation of multiple signal pathways, and hence can be a novel target for pancreatic cancer therapy.