The Experts below are selected from a list of 150 Experts worldwide ranked by ideXlab platform
Colette Charpin - One of the best experts on this subject based on the ideXlab platform.
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Effects of progestins of human proliferative Endometrium: an in vitro model of potential clinical relevance.
International journal of molecular medicine, 2003Co-Authors: Séverine Illouz, Jean-philippe Dales, Karine Sferlazzo, Stéphane Garcia, Séverine Carpentier-meunier, Léon Boubli, Marie-noëlle Lavaut, Colette CharpinAbstract:Endometrium Biopsy is a useful indicator of Endometrium proliferation and is clinically relevant to diagnose cell proliferation and to evaluate response to progestin treatment and to monitor hormone replacement therapy. The aim of our study was to investigate the in vitro effects of progesterone and synthetic progestins on Endometrium explants with a particular focus on estradiol receptor (ER) and progesterone receptor (PR) expression which reflects through cell secretion the hormone treatment efficiency. Most widely used progestagens belonging to three distinctive groups were investigated, i.e, medroxyprogesterone acetate (MPA), norethindrone acetate (NOR) and nomegestrol acetate (TX) which are respectively pregnane, 19-nortestosterone and norpregnane derivatives. We used organ culture from human proliferative Endometrium, in which tissue integrity, particularly gland/stroma relationships are preserved. Progestins induce epithelial cell secretion and most effects were observed at the highest concentration tested (10(-7) M) and by TX and MPA on homogeneous and on heterogeneous (including also secretory glands) proliferative Endometrium respectively. In these conditions, ER as well as PR expression were decreased on both glandular and stromal cells. In contrast, progesterone at 10(-7) M significantly decreased only PR, in glands and in stroma of homogeneous proliferative Endometrium, and just in stroma of heterogeneous Endometrium. NOR exhibited less effects. At lower concentrations (10(-8) M, 10(-9) M), significantly less effects were observed by synthetic progestins on proliferative Endometrium. The experiments show that the different types of progestins do not exhibit in vitro similar effects. Since progestins variably act on proliferative Endometrium, the exposure of Endometrium explants to progestins may be a useful tool to predict clinical response to hormone therapy (individual "hormonogram") and to monitor Endometrium proliferation.
Séverine Illouz - One of the best experts on this subject based on the ideXlab platform.
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Effects of progestins of human proliferative Endometrium: an in vitro model of potential clinical relevance.
International journal of molecular medicine, 2003Co-Authors: Séverine Illouz, Jean-philippe Dales, Karine Sferlazzo, Stéphane Garcia, Séverine Carpentier-meunier, Léon Boubli, Marie-noëlle Lavaut, Colette CharpinAbstract:Endometrium Biopsy is a useful indicator of Endometrium proliferation and is clinically relevant to diagnose cell proliferation and to evaluate response to progestin treatment and to monitor hormone replacement therapy. The aim of our study was to investigate the in vitro effects of progesterone and synthetic progestins on Endometrium explants with a particular focus on estradiol receptor (ER) and progesterone receptor (PR) expression which reflects through cell secretion the hormone treatment efficiency. Most widely used progestagens belonging to three distinctive groups were investigated, i.e, medroxyprogesterone acetate (MPA), norethindrone acetate (NOR) and nomegestrol acetate (TX) which are respectively pregnane, 19-nortestosterone and norpregnane derivatives. We used organ culture from human proliferative Endometrium, in which tissue integrity, particularly gland/stroma relationships are preserved. Progestins induce epithelial cell secretion and most effects were observed at the highest concentration tested (10(-7) M) and by TX and MPA on homogeneous and on heterogeneous (including also secretory glands) proliferative Endometrium respectively. In these conditions, ER as well as PR expression were decreased on both glandular and stromal cells. In contrast, progesterone at 10(-7) M significantly decreased only PR, in glands and in stroma of homogeneous proliferative Endometrium, and just in stroma of heterogeneous Endometrium. NOR exhibited less effects. At lower concentrations (10(-8) M, 10(-9) M), significantly less effects were observed by synthetic progestins on proliferative Endometrium. The experiments show that the different types of progestins do not exhibit in vitro similar effects. Since progestins variably act on proliferative Endometrium, the exposure of Endometrium explants to progestins may be a useful tool to predict clinical response to hormone therapy (individual "hormonogram") and to monitor Endometrium proliferation.
Jitendra Kumar Tiwari - One of the best experts on this subject based on the ideXlab platform.
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Use of ESAT-6 Polymerase Chain Reaction for rapid diagnosis of female genital tuberculosis
International journal of scientific research, 2016Co-Authors: Ruchi Singh, Veena Sharma, Bharti Malhotra, Pratibha Sharma, Aradhana Chauhan, Jitendra Kumar TiwariAbstract:Background: Female Genital Tuberculosis (FGTB) has been a diagnostic dilemma for clinicians due to the latency of the organism, asymptomatic and varied presentation in majority of the cases and paucity of an accurate diagnostic modality. Culture methods are still the gold standard in the detection of genital tuberculosis (TB) but slow growth of mycobacteriaresults in unacceptable delay in diagnosis and treatment. Moreover, acid fast bacilli (AFB) culture on Lowenstein-Jensen (LJ) medium has a low detection rate. Polymerase Chain Reaction (PCR) based molecular methods are rapid and sensitive in diagnosing TB infection. Aims and Objectives: In the present study,we aimed to use ESAT-6 based PCR for detection of female genital TB and to comparethe results with conventional methods. Materials and Methods: Endometrium Biopsy samples from 50 suspected cases of FGTB from June 2012 to August2012 were processed by modified petroff’s method and the deposit was used for detection of mycobacteria by AFB smear, AFB culture on both solid LJ and liquid Middle brook-7H9 (MB7H9) media and ESAT-6 based PCR. Results of PCR were compared with those of conventional methods. Results: Out of 50 samples received, a total of 36% (18/50) were positive by either of the methods used. Overall positivity by AFB smear microscopy was 2% (1/50) and 10% (5/50) by MB7H9 liquid culture, 8% (4/50) by LJ solid culture and 20% (10/50) by ESAT-6 based PCR. Conclusion: PCR is very rapid and sensitive technique for the diagnosis of genital tuberculosis and has utility in providing the results in clinically relevant time and thereby helping to initiate the treatment timely.
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Rapid Diagnosis of Female Genital Tuberculosis by MPB64 Polymerase Chain Reaction
Indian journal of applied research, 2016Co-Authors: Ruchi Singh, Veena Sharma, Bharti Malhotra, Pratibha Sharma, Aradhana Chauhan, Jitendra Kumar TiwariAbstract:Background: Female Genital Tuberculosis (FGTB) is the main cause of infertility in about 5–16% of cases among women in India. However the exact incidence of FGTB remains unknown, as the majority of the cases remain undiagnosed due to asymptomatic presentation and paucity of investigations.Though various options are available for confirming the diagnosis of tuberculosis (TB), conventional techniques lack the sensitivity and are often time consuming. Polymerase Chain Reaction (PCR) based molecular methods are rapid and sensitive in diagnosing TB infection. Aims and Objectives: We aimed to use MPB64 PCR for detection of female genital TB and to comparethe results with conventional methods. Materials and Methods: 50 Endometrium Biopsy samples from suspected cases of FGTB from June 2012to August2012 were processed by modified petroff’s method. The deposit was used for detection of mycobacteria by Acid Fast Bacilli (AFB) smear, AFB culture on solid and liquid media and MPB64 based PCR. Results of PCR were compared with those of conventional methods. Results: Out of 50 samples received, a total of 36% (18/50) were positive by either of the methods used. Overall positivity by AFB smear microscopy was 2% (1/50) and 10% (5/50) by Middle brook-7H9 (MB7H9) liquid culture, 8% (4/50) by Lowenstein-Jensen (LJ) solid culture and 24% (12/50) by MPB64 based PCR. Conclusion: PCR is very rapid and sensitive technique for the diagnosis of genital tuberculosis. Due to the short turn-around time of PCR, it has utility in providing the results in clinically relevant time and thus helps to initiate the treatment timely.
Marie-noëlle Lavaut - One of the best experts on this subject based on the ideXlab platform.
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Effects of progestins of human proliferative Endometrium: an in vitro model of potential clinical relevance.
International journal of molecular medicine, 2003Co-Authors: Séverine Illouz, Jean-philippe Dales, Karine Sferlazzo, Stéphane Garcia, Séverine Carpentier-meunier, Léon Boubli, Marie-noëlle Lavaut, Colette CharpinAbstract:Endometrium Biopsy is a useful indicator of Endometrium proliferation and is clinically relevant to diagnose cell proliferation and to evaluate response to progestin treatment and to monitor hormone replacement therapy. The aim of our study was to investigate the in vitro effects of progesterone and synthetic progestins on Endometrium explants with a particular focus on estradiol receptor (ER) and progesterone receptor (PR) expression which reflects through cell secretion the hormone treatment efficiency. Most widely used progestagens belonging to three distinctive groups were investigated, i.e, medroxyprogesterone acetate (MPA), norethindrone acetate (NOR) and nomegestrol acetate (TX) which are respectively pregnane, 19-nortestosterone and norpregnane derivatives. We used organ culture from human proliferative Endometrium, in which tissue integrity, particularly gland/stroma relationships are preserved. Progestins induce epithelial cell secretion and most effects were observed at the highest concentration tested (10(-7) M) and by TX and MPA on homogeneous and on heterogeneous (including also secretory glands) proliferative Endometrium respectively. In these conditions, ER as well as PR expression were decreased on both glandular and stromal cells. In contrast, progesterone at 10(-7) M significantly decreased only PR, in glands and in stroma of homogeneous proliferative Endometrium, and just in stroma of heterogeneous Endometrium. NOR exhibited less effects. At lower concentrations (10(-8) M, 10(-9) M), significantly less effects were observed by synthetic progestins on proliferative Endometrium. The experiments show that the different types of progestins do not exhibit in vitro similar effects. Since progestins variably act on proliferative Endometrium, the exposure of Endometrium explants to progestins may be a useful tool to predict clinical response to hormone therapy (individual "hormonogram") and to monitor Endometrium proliferation.
Léon Boubli - One of the best experts on this subject based on the ideXlab platform.
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Effects of progestins of human proliferative Endometrium: an in vitro model of potential clinical relevance.
International journal of molecular medicine, 2003Co-Authors: Séverine Illouz, Jean-philippe Dales, Karine Sferlazzo, Stéphane Garcia, Séverine Carpentier-meunier, Léon Boubli, Marie-noëlle Lavaut, Colette CharpinAbstract:Endometrium Biopsy is a useful indicator of Endometrium proliferation and is clinically relevant to diagnose cell proliferation and to evaluate response to progestin treatment and to monitor hormone replacement therapy. The aim of our study was to investigate the in vitro effects of progesterone and synthetic progestins on Endometrium explants with a particular focus on estradiol receptor (ER) and progesterone receptor (PR) expression which reflects through cell secretion the hormone treatment efficiency. Most widely used progestagens belonging to three distinctive groups were investigated, i.e, medroxyprogesterone acetate (MPA), norethindrone acetate (NOR) and nomegestrol acetate (TX) which are respectively pregnane, 19-nortestosterone and norpregnane derivatives. We used organ culture from human proliferative Endometrium, in which tissue integrity, particularly gland/stroma relationships are preserved. Progestins induce epithelial cell secretion and most effects were observed at the highest concentration tested (10(-7) M) and by TX and MPA on homogeneous and on heterogeneous (including also secretory glands) proliferative Endometrium respectively. In these conditions, ER as well as PR expression were decreased on both glandular and stromal cells. In contrast, progesterone at 10(-7) M significantly decreased only PR, in glands and in stroma of homogeneous proliferative Endometrium, and just in stroma of heterogeneous Endometrium. NOR exhibited less effects. At lower concentrations (10(-8) M, 10(-9) M), significantly less effects were observed by synthetic progestins on proliferative Endometrium. The experiments show that the different types of progestins do not exhibit in vitro similar effects. Since progestins variably act on proliferative Endometrium, the exposure of Endometrium explants to progestins may be a useful tool to predict clinical response to hormone therapy (individual "hormonogram") and to monitor Endometrium proliferation.