The Experts below are selected from a list of 501 Experts worldwide ranked by ideXlab platform
Hugh J Freeman - One of the best experts on this subject based on the ideXlab platform.
-
prevalence of celiac disease in collagenous and lymphocytic colitis
Canadian Journal of Gastroenterology & Hepatology, 2000Co-Authors: H R Gillett, Hugh J FreemanAbstract:Both collagenous and lymphocytic colitis have been described in patients with celiac disease, suggesting an association between the conditions. Over the past few years, the availability, sensitivity and specificity of serological markers for celiac disease have improved - the most recent advancement being the description of tissue transglutaminase as the major antigen for Endomysium antibody. A quantitative ELISA was used to measure titres of immunoglobulin A (IgA) antibody to tissue transglutaminase (tTG) along with an immunofluorescent technique for IgA Endomysium antibody (EmA) in 15 patients with lymphocytic colitis and eight with collagenous colitis to determine whether celiac disease latency could be detected. One patient with lymphocytic colitis demonstrated both elevated titres of tTG antibody and positive EmA, and small bowel biopsy confirmed celiac disease. One patient with collagenous colitis had a slightly elevated titre of tTG antibody with a negative EmA, and results of a small bowel biopsy were normal. Three other patients with lymphocytic colitis were already treated for previously diagnosed celiac disease. The prevalence of celiac disease occurring in lymphocytic colitis was found to be 27%, but no cases of celiac disease in association with collagenous colitis were found.
-
prevalence of iga antibodies to Endomysium and tissue transglutaminase in primary biliary cirrhosis
Canadian Journal of Gastroenterology & Hepatology, 2000Co-Authors: H R Gillett, Karen Cauchdudek, Jenny E L Healthcote, Hugh J FreemanAbstract:The association between celiac disease and primary biliary cirrhosis has been described in several case reports and small screening studies, with varying prevalence rates. Stored sera from 378 patients with primary biliary cirrhosis were tested for immunoglobulin (Ig) A Endomysium and tissue transglutaminase antibodies. Ten patients were positive for both antibodies (2.6%); five of these patients had had small bowel biopsies confirming celiac disease. A further 44 patients (11.6%) had raised titres of IgA tissue transglutaminase antibody but were negative for IgA Endomysium antibody. The increased prevalence of celiac-related antibodies in patients with primary biliary cirrhosis suggests that the two conditions are associated, although the reason for the association remains unclear. Patients with primary biliary cirrhosis should be considered to be at high risk for celiac disease. Although liver biochemistry does not improve when these patients are fed a gluten-free diet, the complications of untreated celiac disease warrant the identification and treatment of the condition in this population.
-
comparison of iga Endomysium antibody and iga tissue transglutaminase antibody in celiac disease
Canadian Journal of Gastroenterology & Hepatology, 2000Co-Authors: H R Gillett, Hugh J FreemanAbstract:The antigen for immunoglobulin (Ig)AEndomysium antibody (EmA), a sensitive and specific serological marker for celiac disease, has recently been described as tissue transglutaminase (tTG). The aim of this study was to compare the assays used to measure IgAEmAand IgA tTG antibody in patients with celiac disease and disease control subjects. Sera from 21 patients with untreated celiac disease, 48 patients with treated celiac disease and 128 disease control subjects were tested both for IgA EmA with the use of indirect immunofluorescence against human umbilical cord and for IgA tTG antibody with the use of ELISA. Titres of IgA tTG antibody were significantly higher in both the untreated and treated celiac groups than in the disease control group. Titres in the treated group were, however, significantly lower than in the untreated group. A reference range was calculated to include 99.8% of the disease control group in whom small bowel biopsy showed no evidence of celiac disease. One patient from the disease control group with raised IgA tTG antibody titres and positive IgA EmA was found to have celiac disease on small bowel biopsy. The sensitivity, specificity, and positive and negative predictive values of the IgA EmA assay were all 100%. The sensitivity of the IgA tTG antibody assay was 95%, specificity 100%, positive predictive value 100% and negative predictive value 97.7%. An ELISA used to measure IgA tTG antibody is an excellent tool to screen for celiac disease and may prove useful for monitoring response to treatment.
W Amara - One of the best experts on this subject based on the ideXlab platform.
-
iga anti Endomysium antibody test a comparison between monkey oesophagus and human umbilical cord as substrate in indirect immuno uorescence test
1998Co-Authors: W AmaraAbstract:AmaraW, Husebekk A. Improved method for serological testing in celiac disease: IgA anti-Endomysium antibody testa comparison between monkey oesophagus and human umbilical cord as substrate in indirect immuno-£uorescence test. Scand J Clin Lab Invest 1998; 58: 547^554. The IgA anti-Endomysium antibody (IgA-EmA) test has been studied using the indirect immuno£uorescence (IF) method with human umbilical cord (HUC) as substrate; 247 sera from patients investigated for celiac disease were tested. The results were in accordance with those obtained using monkey oesophagus (MOE) as substrate. IgA-EmA testing using HUC is shown to have similar reproducibil- ity, sensitivity and speci¢city as MOE. By avoiding both ¢xation in chloroform and the blocking step, the processing time for this improved method is decreased by almost1h, and the results are stable for reading by £uorescence microscopy for at least 24 h. The indirect IF method using HUC as substrate is easy, reliable and inexpensive.We conclude that the IgA-EmA test using HUC as a substrate can be routinely used for celiac disease screening and follow up.
-
iga anti Endomysium antibody test a comparison between monkey oesophagus and human umbilical cord as substrate in indirect immuno uorescence test
1998Co-Authors: W AmaraAbstract:AmaraW, Husebekk A. Improved method for serological testing in celiac disease: IgA anti-Endomysium antibody testa comparison between monkey oesophagus and human umbilical cord as substrate in indirect immuno-£uorescence test. Scand J Clin Lab Invest 1998; 58: 547^554. The IgA anti-Endomysium antibody (IgA-EmA) test has been studied using the indirect immuno£uorescence (IF) method with human umbilical cord (HUC) as substrate; 247 sera from patients investigated for celiac disease were tested. The results were in accordance with those obtained using monkey oesophagus (MOE) as substrate. IgA-EmA testing using HUC is shown to have similar reproducibil- ity, sensitivity and speci¢city as MOE. By avoiding both ¢xation in chloroform and the blocking step, the processing time for this improved method is decreased by almost1h, and the results are stable for reading by £uorescence microscopy for at least 24 h. The indirect IF method using HUC as substrate is easy, reliable and inexpensive.We conclude that the IgA-EmA test using HUC as a substrate can be routinely used for celiac disease screening and follow up.
-
improved method for serological testing in celiac disease iga anti Endomysium antibody test a comparison between monkey oesophagus and human umbilical cord as substrate in indirect immunofluorescence test
Scandinavian Journal of Clinical & Laboratory Investigation, 1998Co-Authors: W Amara, A HusebekkAbstract:The IgA anti-Endomysium antibody (IgA-EmA) test has been studied using the indirect immunofluorescence (IF) method with human umbilical cord (HUC) as substrate; 247 sera from patients investigated for celiac disease were tested. The results were in accordance with those obtained using monkey oesophagus (MOE) as substrate. IgA-EmA testing using HUC is shown to have similar reproducibility, sensitivity and specificity as MOE. By avoiding both fixation in chloroform and the blocking step, the processing time for this improved method is decreased by almost 1 h, and the results are stable for reading by fluorescence microscopy for at least 24 h. The indirect IF method using HUC as substrate is easy, reliable and inexpensive. We conclude that the IgA-EmA test using HUC as a substrate can be routinely used for celiac disease screening and follow up.
H R Gillett - One of the best experts on this subject based on the ideXlab platform.
-
Increased prevalence of celiac disease in girls with Turner syndrome detected using antibodies to Endomysium and tissue transglutaminase
2020Co-Authors: Mrcpuk P M Gillett, H R Gillett, M D Mrcpuk, Dm †, Israel Md Frcpc, D L Metzger, M Faap D Frcpc, Md L Stewart, Md J-p Chanoine, Hj FreemanAbstract:ORIGINAL ARTICLE PM Gillett, HR Gillett, DM Israel, et al. Increased prevalence of celiac disease in girls with Turner syndrome detected using antibodies to Endomysium and tissue transglutaminase. Can J Gastroenterol 2000;14(11):915-918. OBJECTIVE: To establish the prevalence of celiac disease (CD) in girls with Turner syndrome (TS) in British Columbia. METHODS: Forty-five girls with TS were prospectively screened for CD using blinded testing with the current 'gold standard' -immunoglobulin A (IgA) Endomysium antibody (EmA) and the novel IgA tissue transglutaminase antibody (tTG). Those with positive results were offered small bowel biopsies, and a gluten-free diet was recommended if CD was confirmed. RESULTS: One asymptomatic prepubertal East Indian girl was positive for EmA, had an elevated tTG concentration of 560 U/mL and histological evidence of CD. Seven girls were negative for EmA but had elevated tTG concentrations (175 to 250 U/mL); five were white, one was Asian and one was East Indian. Small bowel biopsies were performed on three girls, and the histologies were normal. The remaining four patients declined biopsy. CONCLUSIONS: One girl with TS was identified with CD from 45 screened, giving an overall biopsy-confirmed prevalence of 2.2%. This study confirms previous observations placing girls with TS at higher risk for CD and suggests a similar high prevalence in British Columbia
-
prevalence of celiac disease in collagenous and lymphocytic colitis
Canadian Journal of Gastroenterology & Hepatology, 2000Co-Authors: H R Gillett, Hugh J FreemanAbstract:Both collagenous and lymphocytic colitis have been described in patients with celiac disease, suggesting an association between the conditions. Over the past few years, the availability, sensitivity and specificity of serological markers for celiac disease have improved - the most recent advancement being the description of tissue transglutaminase as the major antigen for Endomysium antibody. A quantitative ELISA was used to measure titres of immunoglobulin A (IgA) antibody to tissue transglutaminase (tTG) along with an immunofluorescent technique for IgA Endomysium antibody (EmA) in 15 patients with lymphocytic colitis and eight with collagenous colitis to determine whether celiac disease latency could be detected. One patient with lymphocytic colitis demonstrated both elevated titres of tTG antibody and positive EmA, and small bowel biopsy confirmed celiac disease. One patient with collagenous colitis had a slightly elevated titre of tTG antibody with a negative EmA, and results of a small bowel biopsy were normal. Three other patients with lymphocytic colitis were already treated for previously diagnosed celiac disease. The prevalence of celiac disease occurring in lymphocytic colitis was found to be 27%, but no cases of celiac disease in association with collagenous colitis were found.
-
prevalence of iga antibodies to Endomysium and tissue transglutaminase in primary biliary cirrhosis
Canadian Journal of Gastroenterology & Hepatology, 2000Co-Authors: H R Gillett, Karen Cauchdudek, Jenny E L Healthcote, Hugh J FreemanAbstract:The association between celiac disease and primary biliary cirrhosis has been described in several case reports and small screening studies, with varying prevalence rates. Stored sera from 378 patients with primary biliary cirrhosis were tested for immunoglobulin (Ig) A Endomysium and tissue transglutaminase antibodies. Ten patients were positive for both antibodies (2.6%); five of these patients had had small bowel biopsies confirming celiac disease. A further 44 patients (11.6%) had raised titres of IgA tissue transglutaminase antibody but were negative for IgA Endomysium antibody. The increased prevalence of celiac-related antibodies in patients with primary biliary cirrhosis suggests that the two conditions are associated, although the reason for the association remains unclear. Patients with primary biliary cirrhosis should be considered to be at high risk for celiac disease. Although liver biochemistry does not improve when these patients are fed a gluten-free diet, the complications of untreated celiac disease warrant the identification and treatment of the condition in this population.
-
comparison of iga Endomysium antibody and iga tissue transglutaminase antibody in celiac disease
Canadian Journal of Gastroenterology & Hepatology, 2000Co-Authors: H R Gillett, Hugh J FreemanAbstract:The antigen for immunoglobulin (Ig)AEndomysium antibody (EmA), a sensitive and specific serological marker for celiac disease, has recently been described as tissue transglutaminase (tTG). The aim of this study was to compare the assays used to measure IgAEmAand IgA tTG antibody in patients with celiac disease and disease control subjects. Sera from 21 patients with untreated celiac disease, 48 patients with treated celiac disease and 128 disease control subjects were tested both for IgA EmA with the use of indirect immunofluorescence against human umbilical cord and for IgA tTG antibody with the use of ELISA. Titres of IgA tTG antibody were significantly higher in both the untreated and treated celiac groups than in the disease control group. Titres in the treated group were, however, significantly lower than in the untreated group. A reference range was calculated to include 99.8% of the disease control group in whom small bowel biopsy showed no evidence of celiac disease. One patient from the disease control group with raised IgA tTG antibody titres and positive IgA EmA was found to have celiac disease on small bowel biopsy. The sensitivity, specificity, and positive and negative predictive values of the IgA EmA assay were all 100%. The sensitivity of the IgA tTG antibody assay was 95%, specificity 100%, positive predictive value 100% and negative predictive value 97.7%. An ELISA used to measure IgA tTG antibody is an excellent tool to screen for celiac disease and may prove useful for monitoring response to treatment.
Miklos Sardy - One of the best experts on this subject based on the ideXlab platform.
-
comparison of a tissue transglutaminase elisa with the Endomysium antibody test in the diagnosis of gluten sensitive enteropathy
Zeitschrift Fur Gastroenterologie, 2000Co-Authors: Miklos Sardy, Sarolta Karpati, F Peterfy, K Rasky, Erika Tomsits, T Zagoni, Attila HorvathAbstract:Background: Tissue transglutaminase (tTG) has recently been found to be the major if not the only autoantigen of gluten-sensitive enteropathy (GSE). Objectives: To further determine the significance of this finding for diagnostic (screening) and follow-up purposes, we performed tTG-based ELISAs, and compared the results to the Endomysium antibody test (EMA), Patients: We examined 120 serum samples from patients with celiac disease (CD) including 72 on a gluten-free diet (GFD) and eleven on a gluten challenge. 47 with dermatitis herpetiformis (DH) including 16 on a GFD and one on a gluten challenge, 96 with non-CD gastrointestinal diseases, and 117 with others; i. e. 380 serum samples altogether. Follow-up sera from 13 patients were included. Methods: Results of an ELISA with guinea pig liver tTG were compared with the EMA test using monkey esophagus. Inhibition of endomysial staining was performed with sera positive on the EMA test but negative with the guinea pig tTG ELISA. Results: The specificity and sensitivity of the tTG ELISA are high (98.6% and 92.5%). The serum IgA antibody titers against tTG decrease after introduction of a GFD. In one case, endomysial staining could not be inhibited. Conclusions: Our results show that the guinea pig tTG ELISA is suitable for use as a simple diagnostic, screening and follow-up method for GSE. Further studies are necessary to identify possible additional minor antigens in GSE.
-
recombinant human tissue transglutaminase elisa for the diagnosis of gluten sensitive enteropathy
Clinical Chemistry, 1999Co-Authors: Miklos Sardy, Sarolta Karpati, Uwe Odenthal, Mats Paulsson, Neil SmythAbstract:Background: Tissue transglutaminase (TGc) has recently been identified as the major, if not the sole, autoantigen of gluten-sensitive enteropathy (GSE). We developed and validated an ELISA based on the human recombinant antigen and compared it to existing serological tests for GSE [guinea pig TGc ELISA and Endomysium antibody (EMA) test]. Methods: Human TGc was expressed in the human embryonic kidney cell line 293-EBNA as a C-terminal fusion protein with the eight-amino acid Strep-tag II allowing one-step purification via streptavidin affinity chromatography. We carried out ELISA assays for IgA antibodies against TGc using calcium-activated human and guinea pig TGc. The sera were also tested on monkey esophagus sections by indirect immunofluorescence for IgA EMA. We examined 71 serum samples from patients with GSE (38 with celiac disease, 33 with dermatitis herpetiformis), including 16 on therapy, and 53 controls. Results: The human TGc could be expressed and purified as an active enzyme giving a single band on a Coomassie-stained gel. The mean intra- and interassay CVs for the human TGc ELISA were 3.2% and 9.2%, respectively. The area under the ROC curve was 0.999. The specificity and sensitivity were 98.1% (95% confidence interval, 95.7–100%) and 98.2% (95.9–100%), respectively. Conclusions: The human TGc ELISA was somewhat superior to the guinea pig TGc ELISA, and was as specific and sensitive as the EMA test. The human TGc-based ELISA is the method of choice for easy and noninvasive screening and diagnosis of GSE.
Alessandro Ventura - One of the best experts on this subject based on the ideXlab platform.
-
Usefulness of screening program for celiac disease in autoimmune thyroiditis.
Digestive diseases and sciences, 2000Co-Authors: Irene Berti, Alessandro Ventura, C Trevisiol, Alberto Tommasini, Angelo Città, Elena Neri, Onelio Geatti, Alberto Giammarini, Tarcisio NotAbstract:We determined the prevalence of celiac disease in subjects with autoimmune thyroiditis compared with sick and healthy subjects. The screening was performed with IgA-class Endomysium antibody, by indirect immunofluorescence using human umbilical cord as the antigenic substrate. Six of the 172 patients with autoimmune thyroiditis were found to be anti-Endomysium positive (3.4%) and five of these underwent intestinal biopsy, which showed total villous atrophy. By contrast, 3 (0.75%) of 396 patients with nongastroenterologic malignancies and 10 (0.25%) of 4000 blood donors were found to have celiac disease. The prevalence of autoimmune diseases was significantly higher in patients with both celiac disease and autoimmune thyroiditis than in patients with autoimmune thyroiditis alone (P = 0.01). This study confirms that celiac disease is increased among patients with autoimmune thyroiditis. We suggest that these patients may benefit from screening for celiac disease so as to eliminate symptoms and limit the risk of developing other autoimmune disorders.
-
anti Endomysium antibody on human umbilical cord vein tissue an inexpensive and sensitive diagnostic tool for the screening of coeliac disease
European Journal of Pediatrics, 1997Co-Authors: Anna Citta, Alejandro Lucchesi, Stefano Martelossi, G La Torre, Alessandro VenturaAbstract:Anti-Endomysium antibody (AEA) was evaluated in 136 subjects by indirect immunofluorescence using both cryosections of monkey oesophagus (MO) and the human umbilical cord vein (HUCV) as substrate. This human tissue gave results comparable to those of MO. In particular, the HUCV sections showed positive results in all 22 newly diagnosed cases of coeliac disease. Compared to the MO sections, the sensitivity, specificity and positive predictive values of HUCV tissue was 100%.