The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform

Tadashi Tanabe - One of the best experts on this subject based on the ideXlab platform.

  • characterization of the human gene ptgs2 encoding prostaglandin Endoperoxide synthase 2
    FEBS Journal, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

  • Characterization of the human gene (PTGS2) encoding prostaglandin‐Endoperoxide synthase 2
    European journal of biochemistry, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

Tetsuya Kosaka - One of the best experts on this subject based on the ideXlab platform.

  • characterization of the human gene ptgs2 encoding prostaglandin Endoperoxide synthase 2
    FEBS Journal, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

  • Characterization of the human gene (PTGS2) encoding prostaglandin‐Endoperoxide synthase 2
    European journal of biochemistry, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

Eiichi Takahashi - One of the best experts on this subject based on the ideXlab platform.

  • characterization of the human gene ptgs2 encoding prostaglandin Endoperoxide synthase 2
    FEBS Journal, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

  • Characterization of the human gene (PTGS2) encoding prostaglandin‐Endoperoxide synthase 2
    European journal of biochemistry, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

Atsuro Miyata - One of the best experts on this subject based on the ideXlab platform.

  • characterization of the human gene ptgs2 encoding prostaglandin Endoperoxide synthase 2
    FEBS Journal, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

  • Characterization of the human gene (PTGS2) encoding prostaglandin‐Endoperoxide synthase 2
    European journal of biochemistry, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

Hayato Ihara - One of the best experts on this subject based on the ideXlab platform.

  • characterization of the human gene ptgs2 encoding prostaglandin Endoperoxide synthase 2
    FEBS Journal, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.

  • Characterization of the human gene (PTGS2) encoding prostaglandin‐Endoperoxide synthase 2
    European journal of biochemistry, 1994
    Co-Authors: Tetsuya Kosaka, Atsuro Miyata, Hayato Ihara, Shuntaro Hara, Tamiko Sugimoto, Osamu Takeda, Eiichi Takahashi, Tadashi Tanabe
    Abstract:

    The human gene (PTGS2) encoding an inducible isozyme of prostaglandin-Endoperoxide synthase (prostaglandin-Endoperoxide synthase 2) that is distinct from the well-characterized and constitutive isozyme (prostaglandin-Endoperoxide synthase 1), was isolated using a polymerase-chain reaction-generated cDNA fragment probe for human prostaglandin-Endoperoxide synthase 2. Nucleotide sequence analysis of the entire human prostaglandin-Endoperoxide-synthase-2 gene demonstrated that it is more than 8.3 kb in size and consists of ten exons; this gene is very similar to the murine and chicken prostaglandin-Endoperoxide-synthase-2 genes. The structures of exons in the human prostaglandin-Endoperoxide-synthase-2 gene were also similar to those of the human prostaglandin-Endoperoxide-synthase-1 gene (PTGS1). However, the sizes of introns in the human prostaglandin-Endoperoxide-synthase-2 gene were generally smaller than those of the human prostaglandin-Endoperoxide-synthase-1 gene. Primer-extension analysis indicated that the transcriptional-start site is 134 bases upstream of the translational-initiation site. The sequence of the 1.69-kb region of nucleotides preceding the transcriptional-start site and the first 0.8-kb intron contained a canonical TATA box and various transcriptional-regulatory elements (CArG box, NF-IL6, PEA-1, myb, GATA-1, xenobiotic-response element, cAMP-response element, NF-kappa B, PEA-3, Sp-1 and 12-O-tetradecanoyl-phorbol-13-acetate-response element). The nucleotide sequence of the 5'-flanking region (275 bp) of the human prostaglandin-Endoperoxide-synthase-2 gene showed 63% similarity to the sequence of murine prostaglandin-Endoperoxide-synthase-2/TIS10 gene, but essentially no homology to the chicken prostaglandin-Endoperoxide-synthase-2 gene, and human and murine prostaglandin-Endoperoxide-synthase-1 genes. A fluorescence in situ hybridization study showed that the human genes coding for prostaglandin-Endoperoxide synthase 1 (PTGS1) and prostaglandin-endoperoxidase synthase 2 (PTGS2) were mapped to distinct chromosomes 9q32-q33.3 and 1q25.2-q25.3, respectively, indicating that these genes are not genetically linked.