The Experts below are selected from a list of 18417 Experts worldwide ranked by ideXlab platform
Richard B Meagher - One of the best experts on this subject based on the ideXlab platform.
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Yujing Li, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Richard B MeagherAbstract:Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Jin Shi, Richard B MeagherAbstract:We have developed a genetics-based phytoremediation strategy for arsenic in which the oxyanion arsenate is transported aboveground, reduced to arsenite, and sequestered in thiol-peptide complexes. The Escherichia coli arsC gene encodes arsenate reductase (ArsC), which catalyzes the glutathione (GSH)-coupled electrochemical reduction of arsenate to the more toxic arsenite. Arabidopsis thaliana plants transformed with the arsC gene expressed from a light-induced soybean rubisco promoter (SRS1p) strongly express ArsC protein in leaves, but not roots, and were consequently hypersensitive to arsenate. Arabidopsis plants expressing the E. coli gene encoding gamma-glutamylcysteine synthetase (gamma-ECS) from a strong constitutive actin promoter (ACT2p) were moderately tolerant to arsenic compared with wild type. However, plants expressing SRS1p/ArsC and ACT2p/gamma-ECS together showed substantially greater arsenic Tolerance than gamma-ECS or wild-type plants. When grown on arsenic, these plants accumulated 4- to 17-fold greater fresh shoot weight and accumulated 2- to 3-fold more arsenic per gram of tissue than wild type or plants expressing gamma-ECS or ArsC alone. This arsenic remediation strategy should be applicable to a wide variety of plant species.
Om Parkash Dhankher - One of the best experts on this subject based on the ideXlab platform.
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Yujing Li, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Richard B MeagherAbstract:Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Jin Shi, Richard B MeagherAbstract:We have developed a genetics-based phytoremediation strategy for arsenic in which the oxyanion arsenate is transported aboveground, reduced to arsenite, and sequestered in thiol-peptide complexes. The Escherichia coli arsC gene encodes arsenate reductase (ArsC), which catalyzes the glutathione (GSH)-coupled electrochemical reduction of arsenate to the more toxic arsenite. Arabidopsis thaliana plants transformed with the arsC gene expressed from a light-induced soybean rubisco promoter (SRS1p) strongly express ArsC protein in leaves, but not roots, and were consequently hypersensitive to arsenate. Arabidopsis plants expressing the E. coli gene encoding gamma-glutamylcysteine synthetase (gamma-ECS) from a strong constitutive actin promoter (ACT2p) were moderately tolerant to arsenic compared with wild type. However, plants expressing SRS1p/ArsC and ACT2p/gamma-ECS together showed substantially greater arsenic Tolerance than gamma-ECS or wild-type plants. When grown on arsenic, these plants accumulated 4- to 17-fold greater fresh shoot weight and accumulated 2- to 3-fold more arsenic per gram of tissue than wild type or plants expressing gamma-ECS or ArsC alone. This arsenic remediation strategy should be applicable to a wide variety of plant species.
Julie F Senecoff - One of the best experts on this subject based on the ideXlab platform.
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Yujing Li, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Richard B MeagherAbstract:Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Jin Shi, Richard B MeagherAbstract:We have developed a genetics-based phytoremediation strategy for arsenic in which the oxyanion arsenate is transported aboveground, reduced to arsenite, and sequestered in thiol-peptide complexes. The Escherichia coli arsC gene encodes arsenate reductase (ArsC), which catalyzes the glutathione (GSH)-coupled electrochemical reduction of arsenate to the more toxic arsenite. Arabidopsis thaliana plants transformed with the arsC gene expressed from a light-induced soybean rubisco promoter (SRS1p) strongly express ArsC protein in leaves, but not roots, and were consequently hypersensitive to arsenate. Arabidopsis plants expressing the E. coli gene encoding gamma-glutamylcysteine synthetase (gamma-ECS) from a strong constitutive actin promoter (ACT2p) were moderately tolerant to arsenic compared with wild type. However, plants expressing SRS1p/ArsC and ACT2p/gamma-ECS together showed substantially greater arsenic Tolerance than gamma-ECS or wild-type plants. When grown on arsenic, these plants accumulated 4- to 17-fold greater fresh shoot weight and accumulated 2- to 3-fold more arsenic per gram of tissue than wild type or plants expressing gamma-ECS or ArsC alone. This arsenic remediation strategy should be applicable to a wide variety of plant species.
David E. Salt - One of the best experts on this subject based on the ideXlab platform.
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Yujing Li, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Richard B MeagherAbstract:Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Jin Shi, Richard B MeagherAbstract:We have developed a genetics-based phytoremediation strategy for arsenic in which the oxyanion arsenate is transported aboveground, reduced to arsenite, and sequestered in thiol-peptide complexes. The Escherichia coli arsC gene encodes arsenate reductase (ArsC), which catalyzes the glutathione (GSH)-coupled electrochemical reduction of arsenate to the more toxic arsenite. Arabidopsis thaliana plants transformed with the arsC gene expressed from a light-induced soybean rubisco promoter (SRS1p) strongly express ArsC protein in leaves, but not roots, and were consequently hypersensitive to arsenate. Arabidopsis plants expressing the E. coli gene encoding gamma-glutamylcysteine synthetase (gamma-ECS) from a strong constitutive actin promoter (ACT2p) were moderately tolerant to arsenic compared with wild type. However, plants expressing SRS1p/ArsC and ACT2p/gamma-ECS together showed substantially greater arsenic Tolerance than gamma-ECS or wild-type plants. When grown on arsenic, these plants accumulated 4- to 17-fold greater fresh shoot weight and accumulated 2- to 3-fold more arsenic per gram of tissue than wild type or plants expressing gamma-ECS or ArsC alone. This arsenic remediation strategy should be applicable to a wide variety of plant species.
Nupur A Sashti - One of the best experts on this subject based on the ideXlab platform.
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Yujing Li, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Richard B MeagherAbstract:Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression
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Engineering Tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma glutamylcysteine synthetase expression
Nature Biotechnology, 2002Co-Authors: Om Parkash Dhankher, David E. Salt, Barry P Rosen, Julie F Senecoff, Nupur A Sashti, Jin Shi, Richard B MeagherAbstract:We have developed a genetics-based phytoremediation strategy for arsenic in which the oxyanion arsenate is transported aboveground, reduced to arsenite, and sequestered in thiol-peptide complexes. The Escherichia coli arsC gene encodes arsenate reductase (ArsC), which catalyzes the glutathione (GSH)-coupled electrochemical reduction of arsenate to the more toxic arsenite. Arabidopsis thaliana plants transformed with the arsC gene expressed from a light-induced soybean rubisco promoter (SRS1p) strongly express ArsC protein in leaves, but not roots, and were consequently hypersensitive to arsenate. Arabidopsis plants expressing the E. coli gene encoding gamma-glutamylcysteine synthetase (gamma-ECS) from a strong constitutive actin promoter (ACT2p) were moderately tolerant to arsenic compared with wild type. However, plants expressing SRS1p/ArsC and ACT2p/gamma-ECS together showed substantially greater arsenic Tolerance than gamma-ECS or wild-type plants. When grown on arsenic, these plants accumulated 4- to 17-fold greater fresh shoot weight and accumulated 2- to 3-fold more arsenic per gram of tissue than wild type or plants expressing gamma-ECS or ArsC alone. This arsenic remediation strategy should be applicable to a wide variety of plant species.