The Experts below are selected from a list of 177141 Experts worldwide ranked by ideXlab platform
Pamela A Silver - One of the best experts on this subject based on the ideXlab platform.
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synthetic lipid containing scaffolds Enhance Production by colocalizing enzymes
ACS Synthetic Biology, 2016Co-Authors: Cameron Myhrvold, Jessica K Polka, Pamela A SilverAbstract:Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using colocalization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling coregulation. Synthetic scaffolds extend these benefits to new biological processes and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipid-encapsulated bacteriophage ϕ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by particle tracking in live cells indicates that SLSs are >20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be colocalized to SLSs. To test for effects on enzymatic Production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold...
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synthetic lipid containing scaffolds Enhance Production by co localizing enzymes
bioRxiv, 2016Co-Authors: Cameron Myhrvold, Jessica K Polka, Pamela A SilverAbstract:Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using co-localization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling co-regulation. Synthetic scaffolds extend these benefits to new biological processes, and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipidencapsulated bacteriophage φ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by tracking particles in live cells indicates that SLSs are >20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be co-localized to SLSs. To test for effects on enzymatic Production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold-dependent increase in indigo Production, showing that SLSs can Enhance metabolic reactions.
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engineering acyl carrier protein to Enhance Production of shortened fatty acids
Biotechnology for Biofuels, 2016Co-Authors: Pamela A Silver, Xueliang Liu, Wade Hicks, Jeffrey C WayAbstract:Background The acyl carrier protein (ACP) is an essential and ubiquitous component of microbial synthesis of fatty acids, the natural precursor to biofuels. Natural fatty acids usually contain long chains of 16 or more carbon atoms. Shorter carbon chains, with increased fuel volatility, are desired for internal combustion engines. Engineering the length specificity of key proteins in fatty acid metabolism, such as ACP, may enable microbial synthesis of these shorter chain fatty acids.
Cameron Myhrvold - One of the best experts on this subject based on the ideXlab platform.
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synthetic lipid containing scaffolds Enhance Production by colocalizing enzymes
ACS Synthetic Biology, 2016Co-Authors: Cameron Myhrvold, Jessica K Polka, Pamela A SilverAbstract:Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using colocalization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling coregulation. Synthetic scaffolds extend these benefits to new biological processes and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipid-encapsulated bacteriophage ϕ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by particle tracking in live cells indicates that SLSs are >20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be colocalized to SLSs. To test for effects on enzymatic Production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold...
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synthetic lipid containing scaffolds Enhance Production by co localizing enzymes
bioRxiv, 2016Co-Authors: Cameron Myhrvold, Jessica K Polka, Pamela A SilverAbstract:Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using co-localization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling co-regulation. Synthetic scaffolds extend these benefits to new biological processes, and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipidencapsulated bacteriophage φ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by tracking particles in live cells indicates that SLSs are >20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be co-localized to SLSs. To test for effects on enzymatic Production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold-dependent increase in indigo Production, showing that SLSs can Enhance metabolic reactions.
Ankur B. Dalia - One of the best experts on this subject based on the ideXlab platform.
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multiplex genome editing by natural transformation mugent for synthetic biology in vibrio natriegens
ACS Synthetic Biology, 2017Co-Authors: Triana N. Dalia, Chelsea A Hayes, Sergey Stolyar, Christopher J Marx, James B Mckinlay, Ankur B. DaliaAbstract:Vibrio natriegens has recently emerged as an alternative to Escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. Here, we uncover how to induce natural competence in V. natriegens and describe methods for multiplex genome editing by natural transformation (MuGENT). MuGENT promotes integration of multiple genome edits at high-efficiency on unprecedented time scales. Also, this method allows for generating highly complex mutant populations, which can be exploited for metabolic engineering efforts. As a proof-of-concept, we attempted to Enhance Production of the value added chemical poly-β-hydroxybutyrate (PHB) in V. natriegens by targeting the expression of nine genes involved in PHB biosynthesis via MuGENT. Within 1 week, we isolated edited strains that produced ∼100 times more PHB than the parent isolate and ∼3.3 times more than a rationally designed strain. Thus, the methods described here should extend the utility of this species for diverse ...
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multiplex genome editing by natural transformation mugent for synthetic biology in vibrio natriegens
bioRxiv, 2017Co-Authors: Triana N. Dalia, Chelsea A Hayes, Sergey Stolyar, Christopher J Marx, James B Mckinlay, Ankur B. DaliaAbstract:Vibrio natriegens has recently emerged as an alternative to Escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. Here, we uncover how to induce natural competence in V. natriegens and describe methods for multiplex genome editing by natural transformation (MuGENT). MuGENT promotes integration of multiple genome edits at high-efficiency on unprecedented timescales. Also, this method allows for generating highly complex mutant populations, which can be exploited for metabolic engineering efforts. As a proof-of-concept, we attempted to Enhance Production of the value added chemical poly-β-hydroxybutyrate (PHB) in V. natriegens by targeting the expression of nine genes involved in PHB biosynthesis via MuGENT. Within 1 week, we isolated edited strains that produced ~100 times more PHB than the parent isolate and ~3.3 times more than a rationally designed strain. Thus, the methods described here should extend the utility of this species for diverse academic and industrial applications.
Jessica K Polka - One of the best experts on this subject based on the ideXlab platform.
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synthetic lipid containing scaffolds Enhance Production by colocalizing enzymes
ACS Synthetic Biology, 2016Co-Authors: Cameron Myhrvold, Jessica K Polka, Pamela A SilverAbstract:Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using colocalization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling coregulation. Synthetic scaffolds extend these benefits to new biological processes and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipid-encapsulated bacteriophage ϕ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by particle tracking in live cells indicates that SLSs are >20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be colocalized to SLSs. To test for effects on enzymatic Production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold...
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synthetic lipid containing scaffolds Enhance Production by co localizing enzymes
bioRxiv, 2016Co-Authors: Cameron Myhrvold, Jessica K Polka, Pamela A SilverAbstract:Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using co-localization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling co-regulation. Synthetic scaffolds extend these benefits to new biological processes, and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipidencapsulated bacteriophage φ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by tracking particles in live cells indicates that SLSs are >20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be co-localized to SLSs. To test for effects on enzymatic Production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold-dependent increase in indigo Production, showing that SLSs can Enhance metabolic reactions.
Triana N. Dalia - One of the best experts on this subject based on the ideXlab platform.
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multiplex genome editing by natural transformation mugent for synthetic biology in vibrio natriegens
ACS Synthetic Biology, 2017Co-Authors: Triana N. Dalia, Chelsea A Hayes, Sergey Stolyar, Christopher J Marx, James B Mckinlay, Ankur B. DaliaAbstract:Vibrio natriegens has recently emerged as an alternative to Escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. Here, we uncover how to induce natural competence in V. natriegens and describe methods for multiplex genome editing by natural transformation (MuGENT). MuGENT promotes integration of multiple genome edits at high-efficiency on unprecedented time scales. Also, this method allows for generating highly complex mutant populations, which can be exploited for metabolic engineering efforts. As a proof-of-concept, we attempted to Enhance Production of the value added chemical poly-β-hydroxybutyrate (PHB) in V. natriegens by targeting the expression of nine genes involved in PHB biosynthesis via MuGENT. Within 1 week, we isolated edited strains that produced ∼100 times more PHB than the parent isolate and ∼3.3 times more than a rationally designed strain. Thus, the methods described here should extend the utility of this species for diverse ...
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multiplex genome editing by natural transformation mugent for synthetic biology in vibrio natriegens
bioRxiv, 2017Co-Authors: Triana N. Dalia, Chelsea A Hayes, Sergey Stolyar, Christopher J Marx, James B Mckinlay, Ankur B. DaliaAbstract:Vibrio natriegens has recently emerged as an alternative to Escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. Here, we uncover how to induce natural competence in V. natriegens and describe methods for multiplex genome editing by natural transformation (MuGENT). MuGENT promotes integration of multiple genome edits at high-efficiency on unprecedented timescales. Also, this method allows for generating highly complex mutant populations, which can be exploited for metabolic engineering efforts. As a proof-of-concept, we attempted to Enhance Production of the value added chemical poly-β-hydroxybutyrate (PHB) in V. natriegens by targeting the expression of nine genes involved in PHB biosynthesis via MuGENT. Within 1 week, we isolated edited strains that produced ~100 times more PHB than the parent isolate and ~3.3 times more than a rationally designed strain. Thus, the methods described here should extend the utility of this species for diverse academic and industrial applications.