The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Lars Hestbjerg Hansen - One of the best experts on this subject based on the ideXlab platform.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.
Julie Stenberg Pedersen - One of the best experts on this subject based on the ideXlab platform.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.
Yong Ho Park - One of the best experts on this subject based on the ideXlab platform.
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characterization of a T7 like lytic bacterioPhage φsg jl2 of salmonella enterica serovar gallinarum biovar gallinarum
Applied and Environmental Microbiology, 2008Co-Authors: Hyukjoon Kwon, Jihye Jeong, Se Chang Park, Yong Ho ParkAbstract:T7-like viruses have short noncontractile tails and are members of the family Podoviridae. To date, eight strains have been assigned as Enterobacteria Phage T7 and three strains (T3, T7, and φYeO3-12) have been characterized genomically (http://www.ncbi.nlm.nih.gov/ICTVdb/Ictv/index.htm) (19, 50, 51). Genetic recombination between T7-like viruses infecting different bacterial genera or different species has been demonstrated, and T3 may have evolved from an ancient Phage generated by recombination between yersinioPhages φA1122 and φYeO3-12 (20, 51). Horizontal genetic transfer results in genomic mosaicism of Phages, which hinders their hierarchical classification (22, 37). However, common genetic components and layouts observed among T7-like viruses may support the idea that they crossed a “Darwinian threshold” and have been undergoing vertical evolution (26, 79). Therefore, they may be useful in understanding genetic variations of closely related T7-like Phages during host adaptation. However, current genomic data are insufficient to permit such detailed analysis. Additional genome sequences of closely related T7-like viruses are required to gain insight into their vertical evolution. Fowl typhoid is an acute septicemic disease occurring in adult chickens. The disease is characterized by anemia, leukocytosis, and hemorrhage and is an economically disastrous disease in the poultry industry (53). The causative agent, Salmonella enterica serovar Gallinarum biovar Gallinarum, is classified in serogroup D and is both nonmotile and host adapted (3, 53). Differentiation of serovar Gallinarum biovar Gallinarum from frequent avian serogroup D Salmonella strains, such as S. enterica serovar Gallinarum biovar Pullorum and S. enterica serovar Enteritidis, has been partially successful (33, 34), and differentiation of field strains of serovar Gallinarum biovar Gallinarum from the rough vaccine strain SG-9R has become important because of nationwide vaccination in some countries. The appearance of multidrug-resistant serovar Gallinarum biovar Gallinarum strains in the field has prompted increasing concerns about Phage therapy, similar to other bacterial diseases (5, 30, 35, 64, 68), but candidate Phages that are lytic to broad ranges of serovar Gallinarum biovar Gallinarum strains have never been reported. Fowl typhoid has been reported to spread via the fecal-oral route, but recently, fowl typhoid was reproduced by intratracheal challenge with serovar Gallinarum biovar Gallinarum (4). In this study, we report the basic biological properties and complete genomic sequence of a new Salmonella T7-like virus, φSG-JL2. It is lytic to serovar Gallinarum biovar Gallinarum and has a double-stranded DNA of 38,815 bp with 55 putative genes. Comparative genomic analyses have demonstrated the close relationships of φSG-JL2 with φYeO3-12 from Yersinia enterocolitica O3 and with T3 from Escherichia coli and have provided molecular clues to understand host adaptations of related Phages. The obligate specificity and broad lytic activity of φSG-JL2 may be useful for differentiation of serovar Gallinarum biovar Gallinarum from S. enterica serovar Enteritidis and serovar Gallinarum biovar Pullorum, and the prophylactic efficacy of φSG-JL2 against fowl typhoid was tested with a respiratory model of fowl typhoid.
Amaru Miranda Djurhuus - One of the best experts on this subject based on the ideXlab platform.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.
Alexander Byth Carstens - One of the best experts on this subject based on the ideXlab platform.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.
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isolation and complete genome sequencing of pectobacterium Phage jarilo representing a novel genus of bacterioPhages within the subfamily autographivirinae
bioRxiv, 2020Co-Authors: Julie Stenberg Pedersen, Alexander Byth Carstens, Amaru Miranda Djurhuus, Lars Hestbjerg HansenAbstract:Pectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of Phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium Phage Jarilo, representing a novel genus of bacterioPhages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium Phage Jarilo and Enterobacteria Phage T7, but limited nucleotide similarity is found between Jarilo and other Phages within the subfamily Autographivirinae. We propose Pectobacterium Phage Jarilo as the first member of a new genus of bacterioPhages within the subfamily Autographivirinae.