The Experts below are selected from a list of 4854 Experts worldwide ranked by ideXlab platform

Anatoly P. Gmyl - One of the best experts on this subject based on the ideXlab platform.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in virus protein 3C
    PeerJ, 2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground Enteroviruses are small non-enveloped viruses with a (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds the repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features a low-fidelity proCess, whiCh allows the virus to adapt to the Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting the Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6, 400 nuCleotides), whiCh were present in the NCBI database on February 2017 and analysed the variety and abundanCe of sequenCes in domain d of the repliCative element oriL and in the protein 3C. Results A total of 2,842 full genome sequenCes was analysed. The majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y = U/C, N = any nuCleotide, H = A/C/U). The putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion Despite the suggestion that the RNA-binding tripeptide interaCts with the apiCal region of domain d, they evolve independently in nature. Together, our data indiCate the plastiC evolution of both interplayers of 3C-oriL reCognition.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in protein 3C
    2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground. Enteroviruses are small non-enveloped viruses with (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds the repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features low fidelity, whiCh allows the virus to adapt to the Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting the Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods.To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6400 nuCleotides), whiCh were present in the NCBI database on February 2017 and analysed the variety and abundanCe of sequenCes in domain d of the repliCative element oriL and in the protein 3C. Results.A total of 2,842 full genome sequenCes was analysed. The majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y=U/C, N=any nuCleotide, H=A/C/U). The putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion. Despite the suggestion that the RNA-binding tripeptide interaCts with the apiCal region of domain d, they evolve independently in nature. Together, our data indiCate the plastiC evolution of both interplayers of 3C-oriL reCognition.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in protein 3C
    2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground. Enteroviruses are small non-enveloped viruses with (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features low fidelity that allows virus to adapt to Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods.To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6400 nuCleotides) that were present in the NCBI database on February 2017 and analyzed variety and abundanCe of sequenCes in domain d of repliCative element oriL and in the protein 3C. Results. A total of 2842 full genome sequenCes were analyzed. Majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y=U/C, N=any nuCleotide, H=A/C/U). Putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion. Despite RNA-binding tripeptide is suggested to interaCt with apiCal region of domain d, they evolve independently in nature. Together, our data indiCates plastiC evolution of both interplayers of 3C-oriL reCognition.

  • The 2010 outbreak of poliomyelitis in Tajikistan: epidemiology and lessons learnt.
    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2014
    Co-Authors: Maria L. Yakovenko, Olga E. Ivanova, Anatoly P. Gmyl, A P Ivanov, Maria A Prostova, Olga Y. Baykova, Olga V. Isaeva, G.y. Lipskaya, A K Shakaryan
    Abstract:

    A large outbreak of poliomyelitis, with 463 laboratory-Confirmed and 47 polio-Compatible Cases, took plaCe in 2010 in Tajikistan. PhylogenetiC analysis of the viral VP1 gene suggested a single importation of wild poliovirus type 1 from India in late 2009, its further CirCulation in Tajikistan and expansion into neighbouring Countries, namely Kazakhstan, Russia, Turkmenistan and Uzbekistan. Whole-genome sequenCing of 14 isolates revealed reCombination events with Enterovirus C with Cross-overs within the P2 region. Viruses with one Class of reCombinant genomes Co-CirCulated with the parental virus, and representatives of both Caused paralytiC poliomyelitis. SerologiCal analysis of 327 sera from aCute flaCCid paralysis Cases as well as from patients with other diagnoses and from healthy people demonstrated inadequate immunity against polio in the years preCeding the outbreak. EvidenCe was obtained suggesting that vaCCination against poliomyelitis, in rare Cases, may not prevent the disease. FaCtors Contributing to the peCuliarities of this outbreak are disCussed. The outbreak emphasises the neCessity of Continued vaCCination against polio and the need, at least in risk areas, of quality Control of this vaCCination through well planned serologiCal surveillanCe.

  • Complexes assembled from TMV-derived spheriCal partiCles and entire virions of heterogeneous nature.
    Journal of biomolecular structure & dynamics, 2013
    Co-Authors: E.a. Trifonova, Anatoly P. Gmyl, Nikolai Nikitin, Ekaterina A. Lazareva, Olga Karpova, Joseph Atabekov
    Abstract:

    Previously, we desCribed some struCtural features of spheriCal partiCles (SPs) generated by thermal remodelling of the tobaCCo mosaiC virus. The SPs represent a universal platform that Could bind various proteins. Here, we report that entire isometriC virions of heterogeneous nature bind non-speCifiCally to the SPs. Formaldehyde (FA) was used for Covalent binding of a virus to the SPs surfaCe for stabilizing the SP—virus Complexes. Transmission and high resolution sCanning eleCtron miCrosCopy showed that the SPs surfaCe was Covered with virus partiCles. The arChiteCture of SP–virion Complexes was examined by immunologiC methods. Mean diameters of SPs and SP–human Enterovirus C and SP–Cauliflower mosaiC virus (CaMV) Compositions were determined by nanopartiCle traCking analysis (NTA) in liquid. SignifiCantly, neither free SPs nor individual virions were deteCted by NTA in either FA-Crosslinked or FA-untreated Compositions. Entirely, all virions were bound to the SPs surfaCe and the SP sites within the SP–C...

Marietjie Venter - One of the best experts on this subject based on the ideXlab platform.

  • ReemergenCe of reCombinant vaCCine-derived poliovirus outbreak in MadagasCar.
    Journal of Infectious Diseases, 2008
    Co-Authors: Mala Rakoto-andrianarivelo, Nicksy Gumede, Sophie Jegouic, Jean Balanant, Seta N. Andriamamonjy, Sendraharimanana Rabemanantsoa, Maureen Birmingham, Bakolalao Randriamanalina, Léon Nkolomoni, Marietjie Venter
    Abstract:

    BACKGROUND: After the 2001-2002 poliomyelitis outbreak due to reCombinant vaCCine-derived polioviruses (VDPVs) in the Toliara provinCe of MadagasCar, another outbreak reoCCurred in the same provinCe in 2005. METHODS: We ConduCted epidemiologiCal and virologiCal investigations for eaCh polio Case patient and for their ContaCts. RESULTS: From May to August 2005, a total of 5 Cases of aCute flaCCid paralysis were reported among unvaCCinated or partially vaCCinated Children 2-3 years old. Type-3 or type-2 VDPV was isolated from Case patients and from healthy ContaCts. These strains were Classified into 4 reCombinant lineages that showed Complex mosaiC genomiC struCtures originating from different vaCCine strain serotypes and probably from human Enterovirus C (HEV-C) speCies. GenetiC relatedness Could be observed among these 4 lineages. VaCCination Coverage of the population was very low (

  • ReemergenCe of reCombinant vaCCine-derived poliovirus outbreak in MadagasCar.
    The Journal of infectious diseases, 2008
    Co-Authors: Mala Rakoto-andrianarivelo, Nicksy Gumede, Sophie Jegouic, Jean Balanant, Seta N. Andriamamonjy, Sendraharimanana Rabemanantsoa, Maureen Birmingham, Bakolalao Randriamanalina, Léon Nkolomoni, Marietjie Venter
    Abstract:

    BaCkground. After the 2001–2002 poliomyelitis outbreak due to reCombinant vaCCine-derived polioviruses (VDPVs) in the Toliara provinCe of MadagasCar, another outbreak reoCCurred in the same provinCe in 2005. Methods. We ConduCted epidemiologiCal and virologiCal investigations for eaCh polio Case patient and for their ContaCts. Results. From May to August 2005, a total of 5 Cases of aCute flaCCid paralysis were reported among unvaCCinated or partially vaCCinated Children 2–3 years old. Type-3 or type-2 VDPV was isolated from Case patients and from healthy ContaCts. These strains were Classified into 4 reCombinant lineages that showed Complex mosaiC genomiC struCtures originating from different vaCCine strain serotypes and probably from human Enterovirus C (HEV-C) speCies. GenetiC relatedness Could be observed among these 4 lineages. VaCCination Coverage of the population was very low (50%). ConClusions. The broad distribution of VDPVs in the provinCe and their Close genetiC relationship indiCate intense and rapid CoCirCulation and Coevolution of the vaCCine strains and of their related HEV-C strains. The oCCurrenCe of an outbreak due to VDPV 3 years after a previous outbreak indiCates that a short period with low vaCCination Coverage is enough to Create favorable Conditions for the emergenCe of VDPV in this setting.

Alexander N Lukashev - One of the best experts on this subject based on the ideXlab platform.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in virus protein 3C
    PeerJ, 2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground Enteroviruses are small non-enveloped viruses with a (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds the repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features a low-fidelity proCess, whiCh allows the virus to adapt to the Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting the Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6, 400 nuCleotides), whiCh were present in the NCBI database on February 2017 and analysed the variety and abundanCe of sequenCes in domain d of the repliCative element oriL and in the protein 3C. Results A total of 2,842 full genome sequenCes was analysed. The majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y = U/C, N = any nuCleotide, H = A/C/U). The putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion Despite the suggestion that the RNA-binding tripeptide interaCts with the apiCal region of domain d, they evolve independently in nature. Together, our data indiCate the plastiC evolution of both interplayers of 3C-oriL reCognition.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in protein 3C
    2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground. Enteroviruses are small non-enveloped viruses with (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds the repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features low fidelity, whiCh allows the virus to adapt to the Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting the Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods.To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6400 nuCleotides), whiCh were present in the NCBI database on February 2017 and analysed the variety and abundanCe of sequenCes in domain d of the repliCative element oriL and in the protein 3C. Results.A total of 2,842 full genome sequenCes was analysed. The majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y=U/C, N=any nuCleotide, H=A/C/U). The putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion. Despite the suggestion that the RNA-binding tripeptide interaCts with the apiCal region of domain d, they evolve independently in nature. Together, our data indiCate the plastiC evolution of both interplayers of 3C-oriL reCognition.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in protein 3C
    2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground. Enteroviruses are small non-enveloped viruses with (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features low fidelity that allows virus to adapt to Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods.To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6400 nuCleotides) that were present in the NCBI database on February 2017 and analyzed variety and abundanCe of sequenCes in domain d of repliCative element oriL and in the protein 3C. Results. A total of 2842 full genome sequenCes were analyzed. Majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y=U/C, N=any nuCleotide, H=A/C/U). Putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion. Despite RNA-binding tripeptide is suggested to interaCt with apiCal region of domain d, they evolve independently in nature. Together, our data indiCates plastiC evolution of both interplayers of 3C-oriL reCognition.

  • Novel serotypes 105 and 116 are members of distinCt subgroups of human Enterovirus C.
    Journal of General Virology, 2012
    Co-Authors: Alexander N Lukashev, Gilda Grard, Jan Felix Drexler, Valeria O. Kotova, Elena N. Amjaga, V. I. Reznik, Anatoly P. Gmyl, Raphael Taty Taty, Eric M Leroy
    Abstract:

    The full Coding sequenCes of two novel human Enterovirus (HEV)-C serotypes 105 and 116, sampled in the RepubliC of the Congo in 2010 and in Russia in 2011, were identified in this study. Enterovirus (EV)-105 was Closest to EV-104 in the 5′ NTR and to EV-109 in the Coding genome region. It had the same unConventional 5′ NTR as EV-104 and EV-109. The non-CytopathogeniC EV-116 was phylogenetiCally Close to CoxsaCkievirus (CV)-A1, CV-A19 and CV-A22, whiCh also Cannot be propagated in routinely used Cell Cultures. There were signs of reCombination within this subgroup of HEV-C; however, reCombination with Conventional HEV-C was restriCted, implying partial reproduCtive isolation. As there is also evidenCe of different permissive repliCation systems and distinCt genetiC properties of these subgroups, they may represent subspeCies of the HEV-C speCies or different stages of speCiation.

  • type 1 wild poliovirus and putative Enterovirus 109 in an outbreak of aCute flaCCid paralysis in Congo oCtober november 2010
    Eurosurveillance, 2010
    Co-Authors: Gilda Grard, Jan Felix Drexler, Sonia Etenna Lekanadouki, Melanie Caron, Alexander N Lukashev, Dieudonne Nkoghe, Jeanpaul Gonzalez, Christian Drosten, Eric M Leroy
    Abstract:

    An outbreak of flaCCid paralysis syndrome in adults is ongoing in Congo. MoleCular analysis of faeCal, throat and Cerebrospinal samples identified wildtype 1 poliovirus and an additional Enterovirus C strain related to Enterovirus 109 as the Cause. As of 22 November, the Cumulative number of Cases was 409, of whiCh 169 (41.3%) were fatal. This is one of the largest wild type 1 poliovirus outbreaks ever desCribed assoCiated with an unusually high Case fatality rate.

Mala Rakoto-andrianarivelo - One of the best experts on this subject based on the ideXlab platform.

  • ReemergenCe of reCombinant vaCCine-derived poliovirus outbreak in MadagasCar.
    Journal of Infectious Diseases, 2008
    Co-Authors: Mala Rakoto-andrianarivelo, Nicksy Gumede, Sophie Jegouic, Jean Balanant, Seta N. Andriamamonjy, Sendraharimanana Rabemanantsoa, Maureen Birmingham, Bakolalao Randriamanalina, Léon Nkolomoni, Marietjie Venter
    Abstract:

    BACKGROUND: After the 2001-2002 poliomyelitis outbreak due to reCombinant vaCCine-derived polioviruses (VDPVs) in the Toliara provinCe of MadagasCar, another outbreak reoCCurred in the same provinCe in 2005. METHODS: We ConduCted epidemiologiCal and virologiCal investigations for eaCh polio Case patient and for their ContaCts. RESULTS: From May to August 2005, a total of 5 Cases of aCute flaCCid paralysis were reported among unvaCCinated or partially vaCCinated Children 2-3 years old. Type-3 or type-2 VDPV was isolated from Case patients and from healthy ContaCts. These strains were Classified into 4 reCombinant lineages that showed Complex mosaiC genomiC struCtures originating from different vaCCine strain serotypes and probably from human Enterovirus C (HEV-C) speCies. GenetiC relatedness Could be observed among these 4 lineages. VaCCination Coverage of the population was very low (

  • ReemergenCe of reCombinant vaCCine-derived poliovirus outbreak in MadagasCar.
    The Journal of infectious diseases, 2008
    Co-Authors: Mala Rakoto-andrianarivelo, Nicksy Gumede, Sophie Jegouic, Jean Balanant, Seta N. Andriamamonjy, Sendraharimanana Rabemanantsoa, Maureen Birmingham, Bakolalao Randriamanalina, Léon Nkolomoni, Marietjie Venter
    Abstract:

    BaCkground. After the 2001–2002 poliomyelitis outbreak due to reCombinant vaCCine-derived polioviruses (VDPVs) in the Toliara provinCe of MadagasCar, another outbreak reoCCurred in the same provinCe in 2005. Methods. We ConduCted epidemiologiCal and virologiCal investigations for eaCh polio Case patient and for their ContaCts. Results. From May to August 2005, a total of 5 Cases of aCute flaCCid paralysis were reported among unvaCCinated or partially vaCCinated Children 2–3 years old. Type-3 or type-2 VDPV was isolated from Case patients and from healthy ContaCts. These strains were Classified into 4 reCombinant lineages that showed Complex mosaiC genomiC struCtures originating from different vaCCine strain serotypes and probably from human Enterovirus C (HEV-C) speCies. GenetiC relatedness Could be observed among these 4 lineages. VaCCination Coverage of the population was very low (50%). ConClusions. The broad distribution of VDPVs in the provinCe and their Close genetiC relationship indiCate intense and rapid CoCirCulation and Coevolution of the vaCCine strains and of their related HEV-C strains. The oCCurrenCe of an outbreak due to VDPV 3 years after a previous outbreak indiCates that a short period with low vaCCination Coverage is enough to Create favorable Conditions for the emergenCe of VDPV in this setting.

Maria A Prostova - One of the best experts on this subject based on the ideXlab platform.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in virus protein 3C
    PeerJ, 2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground Enteroviruses are small non-enveloped viruses with a (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds the repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features a low-fidelity proCess, whiCh allows the virus to adapt to the Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting the Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6, 400 nuCleotides), whiCh were present in the NCBI database on February 2017 and analysed the variety and abundanCe of sequenCes in domain d of the repliCative element oriL and in the protein 3C. Results A total of 2,842 full genome sequenCes was analysed. The majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y = U/C, N = any nuCleotide, H = A/C/U). The putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion Despite the suggestion that the RNA-binding tripeptide interaCts with the apiCal region of domain d, they evolve independently in nature. Together, our data indiCate the plastiC evolution of both interplayers of 3C-oriL reCognition.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in protein 3C
    2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground. Enteroviruses are small non-enveloped viruses with (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds the repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features low fidelity, whiCh allows the virus to adapt to the Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting the Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods.To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6400 nuCleotides), whiCh were present in the NCBI database on February 2017 and analysed the variety and abundanCe of sequenCes in domain d of the repliCative element oriL and in the protein 3C. Results.A total of 2,842 full genome sequenCes was analysed. The majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y=U/C, N=any nuCleotide, H=A/C/U). The putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion. Despite the suggestion that the RNA-binding tripeptide interaCts with the apiCal region of domain d, they evolve independently in nature. Together, our data indiCate the plastiC evolution of both interplayers of 3C-oriL reCognition.

  • Independent evolution of tetraloop in Enterovirus oriL repliCative element and its putative binding partners in protein 3C
    2017
    Co-Authors: Maria A Prostova, Alexander N Lukashev, Andrei A. Deviatkin, Irina O Tcelykh, Anatoly P. Gmyl
    Abstract:

    BaCkground. Enteroviruses are small non-enveloped viruses with (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some speCies) binds repliCative element oriL to initiate repliCation. The repliCation of Enteroviruses features low fidelity that allows virus to adapt to Changing environment on the one hand, and requires additional meChanisms to maintain the genome stability on the other. StruCtural disturbanCes in the apiCal region of oriL domain d Can be Compensated by amino aCid substitutions in positions 154 or 156 of 3C (amino aCid numeration Corresponds to poliovirus 3C), thus suggesting Co-evolution of these interaCting sequenCes in nature. The aim of this work was to understand Co-evolution patterns of two interaCting repliCation maChinery elements in Enteroviruses, the apiCal region of oriL domain d and its putative binding partners in the 3C protein. Methods.To evaluate the variability of the domain d loop sequenCe we retrieved all available full Enterovirus sequenCes (>6400 nuCleotides) that were present in the NCBI database on February 2017 and analyzed variety and abundanCe of sequenCes in domain d of repliCative element oriL and in the protein 3C. Results. A total of 2842 full genome sequenCes were analyzed. Majority of domain d apiCal loops were tetraloops, whiCh belonged to Consensus YNHG (Y=U/C, N=any nuCleotide, H=A/C/U). Putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apiCal domain d loop region and, in Contrast to it, was speCies-speCifiC. DisCussion. Despite RNA-binding tripeptide is suggested to interaCt with apiCal region of domain d, they evolve independently in nature. Together, our data indiCates plastiC evolution of both interplayers of 3C-oriL reCognition.

  • The 2010 outbreak of poliomyelitis in Tajikistan: epidemiology and lessons learnt.
    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2014
    Co-Authors: Maria L. Yakovenko, Olga E. Ivanova, Anatoly P. Gmyl, A P Ivanov, Maria A Prostova, Olga Y. Baykova, Olga V. Isaeva, G.y. Lipskaya, A K Shakaryan
    Abstract:

    A large outbreak of poliomyelitis, with 463 laboratory-Confirmed and 47 polio-Compatible Cases, took plaCe in 2010 in Tajikistan. PhylogenetiC analysis of the viral VP1 gene suggested a single importation of wild poliovirus type 1 from India in late 2009, its further CirCulation in Tajikistan and expansion into neighbouring Countries, namely Kazakhstan, Russia, Turkmenistan and Uzbekistan. Whole-genome sequenCing of 14 isolates revealed reCombination events with Enterovirus C with Cross-overs within the P2 region. Viruses with one Class of reCombinant genomes Co-CirCulated with the parental virus, and representatives of both Caused paralytiC poliomyelitis. SerologiCal analysis of 327 sera from aCute flaCCid paralysis Cases as well as from patients with other diagnoses and from healthy people demonstrated inadequate immunity against polio in the years preCeding the outbreak. EvidenCe was obtained suggesting that vaCCination against poliomyelitis, in rare Cases, may not prevent the disease. FaCtors Contributing to the peCuliarities of this outbreak are disCussed. The outbreak emphasises the neCessity of Continued vaCCination against polio and the need, at least in risk areas, of quality Control of this vaCCination through well planned serologiCal surveillanCe.