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Helge B. Bode - One of the best experts on this subject based on the ideXlab platform.
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symbiosis virulence and natural product biosynthesis in Entomopathogenic Bacteria are regulated by a small rna
Nature microbiology, 2020Co-Authors: Nick Neubacher, Nicholas J Tobias, Michaela Huber, Xiaofeng Cai, Timo Glatter, Sacha J Pidot, Timothy P Stinear, Anna Lena Lutticke, Kai Papenfort, Helge B. BodeAbstract:Photorhabdus and Xenorhabdus species have mutualistic associations with nematodes and an Entomopathogenic stage1,2 in their life cycles. In both stages, numerous specialized metabolites are produced that have roles in symbiosis and virulence3,4. Although regulators have been implicated in the regulation of these specialized metabolites3,4, how small regulatory RNAs (sRNAs) are involved in this process is not clear. Here, we show that the Hfq-dependent sRNA, ArcZ, is required for specialized metabolite production in Photorhabdus and Xenorhabdus. We discovered that ArcZ directly base-pairs with the mRNA encoding HexA, which represses the expression of specialized metabolite gene clusters. In addition to specialized metabolite genes, we show that the ArcZ regulon affects approximately 15% of all transcripts in Photorhabdus and Xenorhabdus. Thus, the ArcZ sRNA is crucial for specialized metabolite production in Photorhabdus and Xenorhabdus species and could become a useful tool for metabolic engineering and identification of commercially relevant natural products.
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refining the natural product repertoire in Entomopathogenic Bacteria
Trends in Microbiology, 2018Co-Authors: Nicholas J Tobias, Yiming Shi, Helge B. BodeAbstract:Members of the genera Xenorhabdus and Photorhabdus are capable of producing a huge repertoire of different natural products to support a complex life cycle involving insect pathogenesis and nematode symbiosis. Many of the natural products have direct functions, specifically targeting different facets of nematode development or the insect immune system. These adaptations have allowed the Bacteria to thrive in a unique environment and become highly efficient, versatile insect pathogens. Here, we discuss the ecological advantages afforded to the Bacteria by the acquisition of the gene clusters responsible for producing this repertoire of chemical compounds.
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identification and occurrence of the hydroxamate siderophores aerobactin putrebactin avaroferrin and ochrobactin c as virulence factors from Entomopathogenic Bacteria
Environmental Microbiology, 2017Co-Authors: Merle Hirschmann, Helge B. Bode, Florian GrundmannAbstract:Summary Effective iron acquisition and fine-tuned intracellular iron storage systems are the main prerequisites for a successful host invasion by a pathogen. Bacteria have developed several different strategies to sequester this essential element from their environment, one relies on the secretion of low molecular weight compounds with high affinity for ferric iron, the so-called siderophores. Here we report hydroxamate siderophore structures produced by Entomopathogenic Bacteria of the species Xenorhabdus and Photorhabdus, which are known for their potential to produce bioactive natural products, required for their role as nematode symbiont and insect pathogen. Four siderophores could be identified, namely aerobactin, putrebactin, avaroferrin and ochrobactin C, which was found previously only in marine Bacteria. While the putrebactin and avaroferrin producing biosynthesis gene cluster (BGC) is more widespread and most likely was present in a common ancestor of these Bacteria, the aerobactin and ochrobactin producing BGC was probably taken up by a few strains individually. For aerobactin a role in virulence towards Galleria mellonella larvae is shown. This article is protected by copyright. All rights reserved.
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Entomopathogenic Bacteria use multiple mechanisms for bioactive peptide library design
Nature Chemistry, 2017Co-Authors: Sarah Nowak, Frank Wesche, Iris Bischoff, Robert Furst, Helge B. BodeAbstract:The production of natural product compound libraries has been observed in nature for different organisms such as Bacteria, fungi and plants; however, little is known about the mechanisms generating such chemically diverse libraries. Here we report mechanisms leading to the biosynthesis of the chemically diverse rhabdopeptide/xenortide peptides (RXPs). They are exclusively present in Entomopathogenic Bacteria of the genera Photorhabdus and Xenorhabdus that live in symbiosis with nematodes delivering them to insect prey, which is killed and utilized for nutrition by both nematodes and Bacteria. Chemical diversity of the biologically active RXPs results from a combination of iterative and flexible use of monomodular nonribosomal peptide synthetases including substrate promiscuity, enzyme cross-talk and enzyme stoichiometry as shown by in vivo and in vitro experiments. Together, this highlights several of nature's methods for diversification, or evolution, of natural products and sheds light on the biosynthesis of the bioactive RXPs.
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the global regulators lrp leuo and hexa control secondary metabolism in Entomopathogenic Bacteria
Frontiers in Microbiology, 2017Co-Authors: Yvonne Engel, Carina Windhorst, Heidi Goodrichblair, Helge B. BodeAbstract:Photorhabdus luminescens TTO1 and Xenorhabdus nematophila HGB081 are insect pathogenic Bacteria and producers of various structurally diverse bioactive natural products. In these Entomopathogenic Bacteria we investigated the role of the global regulators Lrp, LeuO and HexA in the production of natural products. Lrp is a general activator of natural product biosynthesis in X. nematophila and for most compounds in TTO1. Microarray analysis confirmed these results in X. nematophila and enabled the identification of additional biosynthesis gene clusters (BGC) regulated by Lrp. Moreover, when promoters of two X. nematophila BGC were analysed, transcriptional activation by Lrp was observed. In contrast, LeuO in X. nematophila and P. luminescens has both repressing and activating features, depending on the natural product examined. Furthermore, heterologous overexpression of leuO from X. nematophila in the closely related X. szentirmaii resulted in overproduction of several natural products including novel compounds. The presented findings could be of importance for establishing a tool for overproduction of secondary metabolites and subsequent identification of novel compounds.
Juan Ferre - One of the best experts on this subject based on the ideXlab platform.
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correction for chakroun et al Bacterial vegetative insecticidal proteins vip from Entomopathogenic Bacteria
Microbiology and Molecular Biology Reviews, 2016Co-Authors: Maissa Chakroun, Nuria Banyuls, Yolanda Bel, Baltasar Escriche, Juan FerreAbstract:Volume 80, no. 2, p. [329–350][1], 2016. Page 341, Table 2: The LC50 values for Vip3Aa58 should be 16, 238, and 2,355 instead of 160, 2,380, and 23,550, respectively, and the LC50 values for Vip3Aa59 should be 19, 275, and 1,626 instead of 190, 2,750, and 16,260, respectively. [1]: /lookup/doi/
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Bacterial vegetative insecticidal proteins vip from Entomopathogenic Bacteria
Microbiology and Molecular Biology Reviews, 2016Co-Authors: Maissa Chakroun, Nuria Banyuls, Yolanda Bel, Baltasar Escriche, Juan FerreAbstract:Entomopathogenic Bacteria produce insecticidal proteins that accumulate in inclusion bodies or parasporal crystals (such as the Cry and Cyt proteins) as well as insecticidal proteins that are secreted into the culture medium. Among the latter are the Vip proteins, which are divided into four families according to their amino acid identity. The Vip1 and Vip2 proteins act as binary toxins and are toxic to some members of the Coleoptera and Hemiptera. The Vip1 component is thought to bind to receptors in the membrane of the insect midgut, and the Vip2 component enters the cell, where it displays its ADP-ribosyltransferase activity against actin, preventing microfilament formation. Vip3 has no sequence similarity to Vip1 or Vip2 and is toxic to a wide variety of members of the Lepidoptera. Its mode of action has been shown to resemble that of the Cry proteins in terms of proteolytic activation, binding to the midgut epithelial membrane, and pore formation, although Vip3A proteins do not share binding sites with Cry proteins. The latter property makes them good candidates to be combined with Cry proteins in transgenic plants (Bacillus thuringiensis-treated crops [Bt crops]) to prevent or delay insect resistance and to broaden the insecticidal spectrum. There are commercially grown varieties of Bt cotton and Bt maize that express the Vip3Aa protein in combination with Cry proteins. For the most recently reported Vip4 family, no target insects have been found yet.
Marcel Kaiser - One of the best experts on this subject based on the ideXlab platform.
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biosynthesis of the antibiotic nematophin and its elongated derivatives in Entomopathogenic Bacteria
Organic Letters, 2017Co-Authors: Xiaofeng Cai, Marcel Kaiser, Victoria L Challinor, Helene Adihou, Peter Grun, Daniela Reimer, Lei Zhao, Helge B. BodeAbstract:Nematophin, a known antibiotic natural product against Staphylococcus aureus for almost 20 years, is produced by all strains of Xenorhabdus nematophila. Despite its simple structure, its biosynthesis was unknown. Its biosynthetic pathway is reported using heterologous production in Escherichia coli. Additionally, the identification, structure elucidation, and biosynthesis of six extended nematophin derivatives from Xenorhabdus PB62.4 carrying an additional valine are reported. Preliminary bioactivity studies suggest a biological role of these compounds in the Bacteria-nematode-insect symbiosis.
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facile synthesis of cyclohexanediones and dialkylresorcinols bioactive natural products from Entomopathogenic Bacteria
ChemInform, 2015Co-Authors: Max Kronenwerth, Marcel Kaiser, Christina Dauth, Iain Pemberton, Helge B. BodeAbstract:The crucial step in the synthesis of cyclohexanediones (VI) is the formation of six-membered ring from α,β-unsaturated ketones (IV) and diethyl malonate, followed by saponification and decarboxylation.
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insect specific production of new gamexpeptides in photorhabdus luminescens tto1 widespread natural products in Entomopathogenic Bacteria
ChemBioChem, 2015Co-Authors: Friederike I Nollmann, Carsten Kegler, Christina Dauth, Geraldine Mulley, Marcel Kaiser, Nicholas R WaterfieldAbstract:Discovery of new natural products by heterologous expression reaches its limits, especially when specific building blocks are missing in the heterologous host or the production medium. Here, we describe the insect-specific production of the new GameXPeptides E-H (5-8) from Photorhabdus luminescens TTO1, which can be produced heterologously from expression of the GameXPeptide synthetase GxpS only upon supplementation of the production media with the missing building blocks, and thus must be regarded as the true natural products under natural conditions.
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facile synthesis of cyclohexanediones and dialkylresorcinols bioactive natural products from Entomopathogenic Bacteria
European Journal of Organic Chemistry, 2014Co-Authors: Max Kronenwerth, Marcel Kaiser, Christina Dauth, Iain Pemberton, Helge B. BodeAbstract:A synthesis for the recently identified, widespread Bacterial natural product classes of dialkylresorcinols and cyclohexanediones was developed. The synthesis route is similar to the biosynthesis route in that the formation of the cyclohexanedione ring results from two parts, as exemplified by the synthesis of the multifunctional isopropylstilbenes identified in Photorhabdus luminescens. Testing of these compounds revealed good bioactivity against Trypanosoma brucei rhodesiense and T. cruzi, the causative agents of sleeping sickness and Chagas disease, respectively
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rhabdopeptides as insect specific virulence factors from Entomopathogenic Bacteria
ChemBioChem, 2013Co-Authors: Daniela Reimer, Kimberly N Cowles, Friederike I Nollmann, Marcel Kaiser, Heidi Goodrichblair, Anna Proschak, Andrea J Dowling, Richard Ffrench Constant, Helge B. BodeAbstract:Six novel linear peptides, named "rhabdopeptides", have been identified in the Entomopathogenic bacterium Xenorhabdus nematophila after the discovery of the corresponding rdp gene cluster by using a promoter trap strategy for the detection of insect-inducible genes. The structures of these rhabdopeptides were deduced from labeling experiments combined with detailed MS analysis. Detailed analysis of an rdp mutant revealed that these compounds participate in virulence towards insects and are produced upon Bacterial infection of a suitable insect host. Furthermore, two additional rhabdopeptide derivatives produced by Xenorhabdus cabanillasii were isolated, these showed activity against insect hemocytes thereby confirming the virulence of this novel class of compounds.
Yonggyun Kim - One of the best experts on this subject based on the ideXlab platform.
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comparative analysis of benzylideneacetone derived compounds on insect immunosuppressive and antimicrobial activities
Korean Journal of Applied Entomology, 2012Co-Authors: Samyeol Seo, Yong Pyo Hong, Wonsu Chun, Yonggyun KimAbstract:Benzylinedeneacetone (BZA) is a Bacterial metabolite which is synthesized by at least two Entomopathogenic Bacteria, namely Xenorhabdus nematophila and Photorhabdus temperata subsp. temperata. It has been shown to possess inhibitory effects on insect cellular and humoral immune responses as well as antimicrobial activities against various species of Bacteria and fungi. However, its relatively high phytotoxicity, and nonsystematic effect have thus far prevented its development into an optimal pesticide. This study screened five different BZA derivatives in order to select an optimal compound, which would have relatively high solubility and low phytotoxicity while retaining sufficient degrees of the immunosuppressive and antimicrobial activities associated with BZA. Hydroxylation of the benzene ring of BZA was found to significantly suppress its immunosuppressive and antimicrobial activities. Transformation of the ketone of BZA by carboxylation also suppressed the inhibitory activities. However, a shortening of the aliphatic chain of BZA into acetate form (4-hydroxyphenylacetic acid: HPA) did not decrease the inhibitory activity. HPA also showed much less phytotoxicity against the hot pepper plant Capsicum annuum, when compared to BZA. This study identified an optimal BZA derivative, which exhibited relatively little phytotoxicity, but retained a high degree of inhibitory activity to suppress insect immune responses and antimicrobial activities against plant pathogens.
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phospholipase a2 inhibitors in Bacterial culture broth enhance pathogenicity of a fungus nomuraea rileyi
Journal of Microbiology, 2012Co-Authors: Junga Park, Yonggyun KimAbstract:An Entomopathogenic fungus, Nomuraea rileyi, was isolated and its identity was confirmed by its internal transcribed spacer DNA sequence. The isolated N. rileyi exhibited a specific pathogenicity to lepidopteran species. This study was focused on enhancing the fungal pathogenicity by using immunosuppressive agents. In response to infection of N. rileyi, Spodoptera exigua larvae significantly induced catalytic activity of phospholipase A2 (PLA2) in three immune-associated tissues, namely hemocytes, fat body, and hemolymph plasma. Furthermore, the infected S. exigua larvae induced transcription of several antimicrobial peptide (AMP) genes. Two Entomopathogenic Bacteria, Xenorhabdus nematophila (Xn) and Photorhabdus temperata subsp. temperata (Ptt), possessed specific PLA2-inhibitory activities and their culture broths significantly inhibited the enzyme activities in hemocytes, fat body, and plasma of S. exigua. In addition, the Bacterial metabolites inhibited transcription of AMP genes in S. exigua that would normally respond to the immune challenge by N. rileyi. The immunosuppressive effect of Xn or Ptt Bacterial broth resulted in significant enhancement of the fungal pathogenicity against late instar larvae of S. exigua and Plutella xylostella. The effect of such a mixture was confirmed by field assay against two lepidopteran species. These results suggest that the Bacterial and fungal mixture can be applied to develop a novel biopesticide to control lepidopteran species.
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differential pathogenicity of two Entomopathogenic Bacteria photorhabdus temperata subsp temperata and xenorhabdus nematophila against the red flour beetle tribolium castaneum
Journal of Asia-pacific Entomology, 2010Co-Authors: Sony Shrestha, Yonggyun KimAbstract:Abstract Two Entomopathogenic Bacteria, Photorhabdus temperata subsp. temperata (Ptt) and Xenorhabdus nematophila (Xn), are symbiotically associated with the nematodes, Heterorhabdis megidis and Steinernema carpocapsae, respectively. There is little information on natural host ranges of the nematodes, but a significant difference in pathogenicity was observed between these two Bacteria against the red flour beetle, Tribolium castaneum, in which Ptt exhibited more than six times higher pathogenicity than Xn. The pathogenic difference was not due to their inhibitory effect on phospholipase A2 activity that is required for expression of immune response of T. castaneum. The culture broths of both Bacterial species had insecticidal activities when injected into the hemocoel. When the Bacterial culture broths were fractionated into aqueous and organic extracts, most insecticidal activity remained in the aqueous extracts. The aqueous extracts of two Bacteria contained proteins which showed different profiles.
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two Entomopathogenic Bacteria xenorhabdus nematophila k1 and photorhabdus temperata subsp temperata anu101 secrete factors enhancing bt pathogenicity against the diamondback moth plutella xylostella
Korean Journal of Applied Entomology, 2009Co-Authors: Samyeol Seo, Yonggyun KimAbstract:Two Entomopathogenic Bacteria, Xenorhabdus nematophila and Photorhabdus temperata subsp. temperata, are known to be potent against the diamondback moth, Plutella xylostella, when the Bacteria are injected into the hemocoel. This study investigated any pathogenic effect of their culture broth on P. xylostella by oral administration. Only culture broth of both Bacterial species did not give enough pathogenic effects by the oral administration. However, when the culture broth was orally treated together with Bacillus thuringiensis (Bt), both cell-free culture broth significantly enhanced Bt pathogenicity against the 3rd instar larvae of P. xylostella. The culture broth was then fractionated into hexane, ethyl acetate, and aqueous extracts. Most synergistic effect on Bt pathogenicity was found in ethyl acetate extracts of both Bacterial species. Thin layer chromatography of these extracts clearly showed that ethyl acetate extracts of both Bacterial culture broths possessed metabolites that were different to those of hexane and aqueous extracts. These results suggest that the both Entomopathogenic Bacteria produce and secrete different factors to give significant synergistic effect on Bt pathogenicity.
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identification of two Entomopathogenic Bacteria from a nematode pathogenic to the oriental beetle blitopertha orientalis
Journal of Microbiology and Biotechnology, 2007Co-Authors: Hae Woong Park, Sony Shrestha, Jiae Seo, Yong Ook Kim, Chul Soo Shin, Yonggyun KimAbstract:A pathogenic nematode, Butlerius sp., was isolated from Oriental beetle, Blitopertha orientalis. The infective juveniles exhibited dose- as well as time-dependent Entomopathogenicity on the larvae of B. orientalis. Two Bacterial species, Providencia vermicola (KACC 91278) and Flavobacterium sp. (KACC 91279), were isolated from the infective juveniles and identified. P. vermicola outnumbered Flavobacterium sp. in the nematode host, in which the colony density of P. vermicola was found to be 21 times higher than that of Flavobacterium sp. However, when the two Bacterial species were cocultured in culture media without the nematode host, they showed similar growth rates. Both Bacteria induced significant Entomopathogenicity against Spodoptera exigua larvae infesting economically important vegetable crops, where P. vermicola was more potent than Flavobacterium sp.
Onilda Santos Da ,silva - One of the best experts on this subject based on the ideXlab platform.
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The great potential of Entomopathogenic Bacteria Xenorhabdus and Photorhabdus for mosquito control : a review
2020Co-Authors: Wellington Junior Da ,silva, Pilz Júnior, Harry Luiz, Heermann Ralf, Onilda Santos Da ,silvaAbstract:The control of insects of medical importance, such as Aedes aegypti and Aedes albopictus are still the only effective way to prevent the transmission of diseases, such as dengue, chikungunya and Zika. Their control is performed mainly using chemical products; however, they often have low specificity to non-target organisms, including humans. Also, studies have reported resistance to the most commonly used insecticides, such as the organophosphate and pyrethroids. Biological control is an ecological and sustainable method since it has a slow rate of insect resistance development. Bacterial species of the genera Xenorhabdus and Photorhabdus have been the target of several research groups worldwide, aiming at their use in agricultural, pharmaceutical and industrial products. This review highlights articles referring to the use of Xenorhabdus and Photorhabdus for insects and especially for mosquito control proposing future ways for their biotechnological applicability. Approximately 24 species of Xenorhabdus and five species of Photorhabdus have been described to have insecticidal properties. These studies have shown genes that are capable of encoding low molecular weight proteins, secondary toxin complexes and metabolites with insecticide activities, as well as antibiotic, fungicidal and antiparasitic molecules. In addition, several species of Xenorhabdus and Photorhabdus showed insecticidal properties against mosquitoes. Therefore, these biological agents can be used in new control methods, and must be, urgently considered in short term, in studies and applications, especially in mosquito control
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Entomopathogenic Bacteria Photorhabdus luminescens as drug source against Leishmania amazonensis.
Parasitology, 2017Co-Authors: Ana Maria Antonello, Ralf Heermann, Thaís Sartori, Pedro R. T. Romão, Ana Paula Folmer Correa, Adriano Brandelli, Luiz Carlos Rodrigues Junior, Alessandra Peres, Onilda Santos Da ,silvaAbstract:Leishmaniasis is a widely spread and zoonotic disease with serious problems as low effectiveness of drugs, emergence of parasite resistance and severe adverse reactions. In recent years, considerable attention has been given to secondary metabolites produced by Photorhabdus luminescens, an Entomopathogenic bacterium. Here, we assessed the leishmanicidal activity of P. luminescens culture fluids. Initially, promastigotes of Leishmania amazonensis were incubated with cell free conditioned medium of P. luminescens and parasite survival was monitored. Different pre-treatments of the conditioned medium revealed that the leishmanicidal activity is due to a secreted peptide smaller than 3 kDa. The Photorhabdus-derived leishmanicidal toxin (PLT) was enriched from conditioned medium and its effect on mitochondrial membrane potential of promastigotes, was determined. Moreover, the biological activity of PLT against amastigotes was evaluated. PLT inhibited the parasite growth and showed significant leishmanicidal activity against promastigote and amastigotes of L. amazonensis. PLT also caused mitochondrial dysfunction in parasites, but low toxicity to mammalian cell and human erythrocytes. Moreover, the anti-amastigote activity was independent of nitric oxide production. In summary, our results highlight that P. luminescens secretes Leishmania-toxic peptide(s) that are promising novel drugs for therapy against leishmaniasis.
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larvicidal and growth inhibitory activity of Entomopathogenic Bacteria culture fluids against aedes aegypti diptera culicidae
Journal of Economic Entomology, 2017Co-Authors: Joao Luiz Rosa Da Silva, Ralf Heermann, Carlos Eugenio Silva, Marisa Da Costa, Fernanda Undurraga Schwalm, Onilda Santos Da ,silvaAbstract:Dengue, Chikungunya, and Zika are important vector-borne diseases, and Aedes aegypti L. is their main transmitter. As the disease management is mainly based on mosquito control strategies, the search for alternative and cost-effective approaches is ongoing. The Gram-negative Bacteria Xenorhabdus nematophila and Photorhabdus luminescens are symbiotically associated with Entomopathogenic nematodes and are highly pathogenic for insect larvae. After we have recently confirmed the toxicity of these Bacteria in Ae. aegypti larvae, we here evaluated the toxic activity of culture fluids on the development of this mosquito species. Larval susceptibility was assessed by exposing larvae to different concentrations of P. luminescens or X. nematophila culture fluids to confirm whether secondary metabolites might cause the mosquitos' death. Xenorhabdus nematophila culture fluid was more effective and stable during the mosquito pathogenicity bioassays compared to that of P. luminescens. Larval mortality started a few hours after exposure of the insects to the fluids. Furthermore, the residual effect of larvicidal activity of X. nematophila fluid persisted at full efficiency for 4 d. Particularly, larval mortality was still higher than 50% for up to 8 d. Exposure of larvae to a sublethal dose of X. nematophila fluid delayed pupation as well as emergence of adult mosquitoes and caused cumulative larval mortality higher than 90% by day 14. Here, we describe for the first time the use of stable culture fluids and therefore secondary metabolites of P. luminescens and X. nematophila as a promising basis for the use as biopesticide for control of Ae. aegypti in the future.
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oral toxicity of photorhabdus luminescens and xenorhabdus nematophila enteroBacteriaceae against aedes aegypti diptera culicidae
Parasitology Research, 2013Co-Authors: Onilda Santos Da ,silva, Geronimo Rodrigues Prado, Joao Luiz Rosa Da Silva, Carlos Eugenio Silva, Marisa Da Costa, Ralf HeermannAbstract:Dengue fever is an important vector-borne disease, mainly transmitted by Aedes aegypti. To date, there are no vaccines or effective drugs available against this arboviral disease. As mosquito control is practically the only method available to control dengue fever, alternative and cost-effective pest control strategies need to be explored. The gram-negative enteric Bacteria Xenorhabdus and Photorhabdus are symbiotically associated with nematode parasites, which themselves are highly pathogenic for insect larvae. Here, we evaluate the oral toxicity of these Entomopathogenic Bacteria in A. aegypti larvae. The susceptibility of larvae (third late or fourth early instars) was assessed by exposing them to suspensions containing Photorhabdus luminescens or Xenorhabdus nematophila, respectively. Two diet treatments were tested with larvae fed on pet food and unfed larvae. After 24 h, larvae began to die when exposed to the Bacteria. Exposure to P. luminescens killed 73 % of the fed and 83 % of the unfed larvae, respectively. In comparison, X. nematophila was less pathogenic, killing 52 % of the larvae in the fed and 42 % in the unfed treatment. Remarkably, cannibalism was observed in all bioassays after exposing larvae to either of the Bacterial species. To our knowledge, this is the first report demonstrating the efficiency of these Entomopathogenic Bacteria for oral A. aegypti killing. Our results provide a promising basis for using these Bacteria as bioinsecticides for mosquito control in the future.