The Experts below are selected from a list of 99 Experts worldwide ranked by ideXlab platform

David H. Broide - One of the best experts on this subject based on the ideXlab platform.

  • compartmentalization of Eosinophil Granulocyte macrophage colony stimulating factor expression in patients with asthma
    The Journal of Allergy and Clinical Immunology, 1996
    Co-Authors: Susan K. Sullivan, David H. Broide
    Abstract:

    Background: In patients with asthma the endobronchial instillation of an allergen induces recruitment of Granulocyte-macrophage colony-stimulating factor (GM-CSF) messenger RNA-positive Eosinophils into the airway. Objective: The goal of the study was to determine whether peripheral blood (as opposed to bronchoalveolar lavage) Eosinophils express GM-CSF mRNA and protein. Methods: We performed in situ hybridization and immunocytochemistry on peripheral blood Eosinophils, obtained at 0, 4, and 24 hours after either inhalation of diluent, inhalation of allergen, or endobronchial instillation of allergen. Results: Each study subject ( n =6) had both an immediate and late-phase response to allergen inhalation but not to diluent inhalation. Allergen, but not diluent, challenge induced a significant increase in the number of peripheral blood Eosinophils at 24 hours. In situ hybridization of peripheral blood Eosinophils with a sulfur 35-labeled GM-CSF RNA riboprobe and immunostaining with a GM-CSF monoclonal antibody revealed that neither pre- nor postchallenge (allergen or diluent) peripheral blood Eosinophils expressed GM-CSF mRNA or protein. In contrast, both bronchoalveolar lavage Eosinophils and mononuclear cells expressed GM-CSF mRNA and protein. Conclusion: These studies suggest that the expression of GM-CSF by Eosinophils after allergen inhalation is compartmentalized to the lungs.

  • Compartmentalization of Eosinophil Granulocyte-macrophage colony-stimulating factor expression in patients with asthma *
    The Journal of allergy and clinical immunology, 1996
    Co-Authors: Susan K. Sullivan, David H. Broide
    Abstract:

    Background: In patients with asthma the endobronchial instillation of an allergen induces recruitment of Granulocyte-macrophage colony-stimulating factor (GM-CSF) messenger RNA-positive Eosinophils into the airway. Objective: The goal of the study was to determine whether peripheral blood (as opposed to bronchoalveolar lavage) Eosinophils express GM-CSF mRNA and protein. Methods: We performed in situ hybridization and immunocytochemistry on peripheral blood Eosinophils, obtained at 0, 4, and 24 hours after either inhalation of diluent, inhalation of allergen, or endobronchial instillation of allergen. Results: Each study subject ( n =6) had both an immediate and late-phase response to allergen inhalation but not to diluent inhalation. Allergen, but not diluent, challenge induced a significant increase in the number of peripheral blood Eosinophils at 24 hours. In situ hybridization of peripheral blood Eosinophils with a sulfur 35-labeled GM-CSF RNA riboprobe and immunostaining with a GM-CSF monoclonal antibody revealed that neither pre- nor postchallenge (allergen or diluent) peripheral blood Eosinophils expressed GM-CSF mRNA or protein. In contrast, both bronchoalveolar lavage Eosinophils and mononuclear cells expressed GM-CSF mRNA and protein. Conclusion: These studies suggest that the expression of GM-CSF by Eosinophils after allergen inhalation is compartmentalized to the lungs.

Susan K. Sullivan - One of the best experts on this subject based on the ideXlab platform.

  • compartmentalization of Eosinophil Granulocyte macrophage colony stimulating factor expression in patients with asthma
    The Journal of Allergy and Clinical Immunology, 1996
    Co-Authors: Susan K. Sullivan, David H. Broide
    Abstract:

    Background: In patients with asthma the endobronchial instillation of an allergen induces recruitment of Granulocyte-macrophage colony-stimulating factor (GM-CSF) messenger RNA-positive Eosinophils into the airway. Objective: The goal of the study was to determine whether peripheral blood (as opposed to bronchoalveolar lavage) Eosinophils express GM-CSF mRNA and protein. Methods: We performed in situ hybridization and immunocytochemistry on peripheral blood Eosinophils, obtained at 0, 4, and 24 hours after either inhalation of diluent, inhalation of allergen, or endobronchial instillation of allergen. Results: Each study subject ( n =6) had both an immediate and late-phase response to allergen inhalation but not to diluent inhalation. Allergen, but not diluent, challenge induced a significant increase in the number of peripheral blood Eosinophils at 24 hours. In situ hybridization of peripheral blood Eosinophils with a sulfur 35-labeled GM-CSF RNA riboprobe and immunostaining with a GM-CSF monoclonal antibody revealed that neither pre- nor postchallenge (allergen or diluent) peripheral blood Eosinophils expressed GM-CSF mRNA or protein. In contrast, both bronchoalveolar lavage Eosinophils and mononuclear cells expressed GM-CSF mRNA and protein. Conclusion: These studies suggest that the expression of GM-CSF by Eosinophils after allergen inhalation is compartmentalized to the lungs.

  • Compartmentalization of Eosinophil Granulocyte-macrophage colony-stimulating factor expression in patients with asthma *
    The Journal of allergy and clinical immunology, 1996
    Co-Authors: Susan K. Sullivan, David H. Broide
    Abstract:

    Background: In patients with asthma the endobronchial instillation of an allergen induces recruitment of Granulocyte-macrophage colony-stimulating factor (GM-CSF) messenger RNA-positive Eosinophils into the airway. Objective: The goal of the study was to determine whether peripheral blood (as opposed to bronchoalveolar lavage) Eosinophils express GM-CSF mRNA and protein. Methods: We performed in situ hybridization and immunocytochemistry on peripheral blood Eosinophils, obtained at 0, 4, and 24 hours after either inhalation of diluent, inhalation of allergen, or endobronchial instillation of allergen. Results: Each study subject ( n =6) had both an immediate and late-phase response to allergen inhalation but not to diluent inhalation. Allergen, but not diluent, challenge induced a significant increase in the number of peripheral blood Eosinophils at 24 hours. In situ hybridization of peripheral blood Eosinophils with a sulfur 35-labeled GM-CSF RNA riboprobe and immunostaining with a GM-CSF monoclonal antibody revealed that neither pre- nor postchallenge (allergen or diluent) peripheral blood Eosinophils expressed GM-CSF mRNA or protein. In contrast, both bronchoalveolar lavage Eosinophils and mononuclear cells expressed GM-CSF mRNA and protein. Conclusion: These studies suggest that the expression of GM-CSF by Eosinophils after allergen inhalation is compartmentalized to the lungs.

Radovan Borojevic - One of the best experts on this subject based on the ideXlab platform.

  • Eosinophil Granulocyte proliferation induced by an intermediate factor generated in the pleural cavity of rats injected with platelet activating factor acether
    International Archives of Allergy and Immunology, 1993
    Co-Authors: Sandra A C Perez, Patricia M R E Silva, Marco A Martins, Marcia C Elcheikh, Renato S B Cordeiro, Radovan Borojevic
    Abstract:

    In previous research, we have observed that intrathoracic administration of platelet-activating factor-acether (PAF) promoted a delayed Eosinophilia in the pleural cavity of rats that lasted for at le

  • Eosinophil Granulocyte proliferation and differentiation in schistosomal granulomas are controlled by two cytokines.
    Laboratory investigation; a journal of technical methods and pathology, 1991
    Co-Authors: Márcia C. El-cheikh, Hélio S. Dutra, Radovan Borojevic
    Abstract:

    In parasitic diseases, Eosinophilia is controlled at the systemic level by soluble, circulating factors. In addition to their medullar production and migration to tissues involved by parasitosis, Eosinophil populations in inflammatory infiltrates may be locally amplified by their in situ proliferation. In granulomas induced in liver tissue by eggs of schistosome worms, Eosinophil proliferation and differentiation are observed. We have shown that they were under control of two cytokines, the activity of which can be demonstrated in supernatants of isolated granulomas maintained in culture for 24 hours. One of them has been identified as interleukin-5. The other one is secreted by adherent cells obtained from periovular granulomas, among which macrophages represent more than 99% of cells. It is considered to correspond to the previously described factor, secreted by inflammatory macrophages mobilized on intraperitoneal glass implants in mice with chronic schistosomiasis. In acute schistosomiasis, the activity of the interleukin-5 was predominant, whereas in the chronic phase of the disease, the stimulation of peripheral eosinopoiesis is taken over by the factor secreted by adherent cells. During the progression from the acute to the chronic phase of schistosomiasis, the immune reactivity of the host is down-regulated by T suppressor lymphocyte circuits. In addition, a redistribution of cellular controls of the host reaction to parasites may act as a complementary mechanism for establishment of the viable equilibrium between host and parasite.

Christer Peterson - One of the best experts on this subject based on the ideXlab platform.

  • Eosinophil granule proteins in cardiopulmonary bypass with and without heparin coating
    The Annals of Thoracic Surgery, 1995
    Co-Authors: Leif Nilsson, Per Venge, Christer Peterson, Jan Borowiec, Stefan Thelin
    Abstract:

    Extracorporeal circulation with exposure of blood to foreign surfaces causes activation of different defense systems, eg, white cells. Several potent mediators are released into plasma, capable of causing harmful effects to different organs, contributing to postoperative morbidity after operations using cardiopulmonary bypass. The Eosinophil Granulocyte has not previously been investigated in this respect. We studied two of its activation products, Eosinophil cationic protein and Eosinophil protein X in coronary bypass patients. In 17 control patients, plasma levels of Eosinophil cationic protein and Eosinophil protein X increased considerably during cardiopulmonary bypass. In 19 patients with heparin-coated cardiopulmonary bypass equipment the levels were significantly reduced, indicating improved biocompatibility of the cardiopulmonary bypass circuit. The heparin-coated surface causes less activation of Eosinophils; also, released Eosinophil cationic protein is bound to the heparinized surface.

  • High concentrations of Eosinophil cationic protein and Eosinophil protein X in Eosinophilic pleural effusions.
    Chest, 1993
    Co-Authors: Tom Pettersson, Henrik Riska, Seppo Sutinen, Matti Klockars, Christer Peterson
    Abstract:

    To analyze the association of the Eosinophil Granulocyte with pleural effusions, we measured the concentrations of two Eosinophil proteins, Eosinophil cationic protein (ECP) and Eosinophil protein X (EPX), in pleural fluid and serum of 92 patients with pleural effusions of various causes. We observed significantly higher ECP and EPX concentrations in Eosinophilic than in nonEosinophilic pleural effusions (p

Jonas Byström - One of the best experts on this subject based on the ideXlab platform.

  • Analysing the Eosinophil cationic protein - a clue to the function of the Eosinophil Granulocyte
    Respiratory Research, 2011
    Co-Authors: Jonas Byström, Kawa Amin, David Bishop-bailey
    Abstract:

    Eosinophil Granulocytes reside in respiratory mucosa including lungs, in the gastro-intestinal tract, and in lymphocyte associated organs, the thymus, lymph nodes and the spleen. In parasitic infections, atopic diseases such as atopic dermatitis and asthma, the numbers of the circulating Eosinophils are frequently elevated. In conditions such as HyperEosinophilic Syndrome (HES) circulating Eosinophil levels are even further raised. Although, Eosinophils were identified more than hundred years ago, their roles in homeostasis and in disease still remain unclear. The most prominent feature of the Eosinophils are their large secondary granules, each containing four basic proteins, the best known being the Eosinophil cationic protein (ECP). This protein has been developed as a marker for Eosinophilic disease and quantified in biological fluids including serum, bronchoalveolar lavage and nasal secretions. Elevated ECP levels are found in T helper lymphocyte type 2 (atopic) diseases such as allergic asthma and allergic rhinitis but also occasionally in other diseases such as bacterial sinusitis. ECP is a ribonuclease which has been attributed with cytotoxic, neurotoxic, fibrosis promoting and immune-regulatory functions. ECP regulates mucosal and immune cells and may directly act against helminth, bacterial and viral infections. The levels of ECP measured in disease in combination with the catalogue of known functions of the protein and its polymorphisms presented here will build a foundation for further speculations of the role of ECP, and ultimately the role of the Eosinophil.

  • Eosinophil cationic protein : expression levels and polymorphisms.
    2002
    Co-Authors: Jonas Byström
    Abstract:

    The Eosinophil cationic protein (ECP) is usually associated with the Eosinophil Granulocyte. In this thesis the presence and production of this protein has been studied in two other cells. The circ ...