The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform
Masazumi Nagai - One of the best experts on this subject based on the ideXlab platform.
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Epithelial cell adhesion efficacy of a novel peptide identified by panning on a smooth titanium surface
International Journal of Oral Science, 2018Co-Authors: Hidemichi Kihara, David M. Kim, Masazumi Nagai, Toshiki Nojiri, Shigemi Nagai, Cliff Lee, Wataru Hatakeyama, Chia-yu Chen, Hisatomo Kondo, John SilvaAbstract:Epithelial Attachment via the basal lamina on the tooth surface provides an important structural defence mechanism against bacterial invasion in combating periodontal disease. However, when considering dental implants, strong Epithelial Attachment does not exist throughout the titanium-soft tissue interface, making soft tissues more susceptible to peri-implant disease. This study introduced a novel synthetic peptide (A10) to enhance Epithelial Attachment. A10 was identified from a bacterial peptide display library and synthesized. A10 and protease-activated receptor 4-activating peptide (PAR4-AP, positive control) were immobilized on commercially pure titanium. The peptide-treated titanium showed high Epithelial cell migration ability during incubation in platelet-rich plasma. We confirmed the development of dense and expanded BL (stained by Ln5) with pericellular junctions (stained by ZO1) on the peptide-treated titanium surface. In an adhesion assay of Epithelial cells on A10-treated titanium, PAR4-AP-treated titanium, bovine root and non-treated titanium, A10-treated titanium and PAR4-AP-treated titanium showed significantly stronger adhesion than non-treated titanium. PAR4-AP-treated titanium showed significantly higher inflammatory cytokine release than non-treated titanium. There was no significant difference in inflammatory cytokine release between A10-treated and non-treated titanium. These results indicated that A10 could induce the adhesion and migration of Epithelial cells with low inflammatory cytokine release. This novel peptide has a potentially useful application that could improve clinical outcomes with titanium implants and abutments by reducing or preventing peri-implant disease.
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function of platelet induced Epithelial Attachment at titanium surfaces inhibits microbial colonization
Journal of Dental Research, 2017Co-Authors: M Maeno, Hidemichi Kihara, Shigemi Nagai, J D Da Silva, S Sugawara, Y Nara, Masazumi NagaiAbstract:The aim of this study was to evaluate the barrier function of platelet-induced Epithelial sheets on titanium surfaces. The lack of functional peri-implant Epithelial sealing with basal lamina (BL) Attachment at the interface of the implant and the adjacent epithelium allows for bacterial invasion, which may lead to peri-implantitis. Although various approaches have been reported to combat bacterial infection by surface modifications to titanium, none of these have been successful in a clinical application. In our previous study, surface modification with protease-activated receptor 4–activating peptide (PAR4-AP), which induced platelet activation and aggregation, was successful in demonstrating Epithelial Attachment via BL and Epithelial sheet formation on the titanium surface. We hypothesized that the platelet-induced Epithelial sheet on PAR4-AP–modified titanium surfaces would reduce bacterial Attachment, penetration, and invasion. Titanium surface was modified with PAR4-AP and incubated with platelet-r...
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establishment of Epithelial Attachment on titanium surface coated with platelet activating peptide
PLOS ONE, 2016Co-Authors: Masazumi Nagai, Shigemi Nagai, Shiho Sugawara, Masahiko Maeno, John D Da Silva, Hisatomo KondoAbstract:The aim of this study was to produce Epithelial Attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of Epithelial sealing at the peri-implant collar. Histologically, Epithelial sealing is recognized as the Attachment of the basement membrane (BM). BM-Attachment is promoted by activated platelet aggregates at surgical wound sites. On the other hand, platelets did not aggregate on smooth titanium, the surface typical of the implant abutment. We then hypothesized that Epithelial BM-Attachment was produced when titanium surface was modified to allow platelet aggregation. Titanium surfaces were coated with a protease activated receptor 4-activating peptide (PAR4-AP). PAR4-AP coating yielded rapid aggregation of platelets on the titanium surface. Platelet aggregates released robust amount of Epithelial chemoattractants (IGF-I, TGF-β) and growth factors (EGF, VEGF) on the titanium surface. Human gingival Epithelial cells, when they were co-cultured on the platelet aggregates, successfully attached to the PAR4-AP coated titanium surface with spread laminin5 positive BM and consecutive staining of the Epithelial tight junction component ZO1, indicating the formation of complete Epithelial sheet. These in-vitro results indicate the establishment of Epithelial BM-Attachment to the titanium surface.
Shigemi Nagai - One of the best experts on this subject based on the ideXlab platform.
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Epithelial cell adhesion efficacy of a novel peptide identified by panning on a smooth titanium surface
International Journal of Oral Science, 2018Co-Authors: Hidemichi Kihara, David M. Kim, Masazumi Nagai, Toshiki Nojiri, Shigemi Nagai, Cliff Lee, Wataru Hatakeyama, Chia-yu Chen, Hisatomo Kondo, John SilvaAbstract:Epithelial Attachment via the basal lamina on the tooth surface provides an important structural defence mechanism against bacterial invasion in combating periodontal disease. However, when considering dental implants, strong Epithelial Attachment does not exist throughout the titanium-soft tissue interface, making soft tissues more susceptible to peri-implant disease. This study introduced a novel synthetic peptide (A10) to enhance Epithelial Attachment. A10 was identified from a bacterial peptide display library and synthesized. A10 and protease-activated receptor 4-activating peptide (PAR4-AP, positive control) were immobilized on commercially pure titanium. The peptide-treated titanium showed high Epithelial cell migration ability during incubation in platelet-rich plasma. We confirmed the development of dense and expanded BL (stained by Ln5) with pericellular junctions (stained by ZO1) on the peptide-treated titanium surface. In an adhesion assay of Epithelial cells on A10-treated titanium, PAR4-AP-treated titanium, bovine root and non-treated titanium, A10-treated titanium and PAR4-AP-treated titanium showed significantly stronger adhesion than non-treated titanium. PAR4-AP-treated titanium showed significantly higher inflammatory cytokine release than non-treated titanium. There was no significant difference in inflammatory cytokine release between A10-treated and non-treated titanium. These results indicated that A10 could induce the adhesion and migration of Epithelial cells with low inflammatory cytokine release. This novel peptide has a potentially useful application that could improve clinical outcomes with titanium implants and abutments by reducing or preventing peri-implant disease.
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function of platelet induced Epithelial Attachment at titanium surfaces inhibits microbial colonization
Journal of Dental Research, 2017Co-Authors: M Maeno, Hidemichi Kihara, Shigemi Nagai, J D Da Silva, S Sugawara, Y Nara, Masazumi NagaiAbstract:The aim of this study was to evaluate the barrier function of platelet-induced Epithelial sheets on titanium surfaces. The lack of functional peri-implant Epithelial sealing with basal lamina (BL) Attachment at the interface of the implant and the adjacent epithelium allows for bacterial invasion, which may lead to peri-implantitis. Although various approaches have been reported to combat bacterial infection by surface modifications to titanium, none of these have been successful in a clinical application. In our previous study, surface modification with protease-activated receptor 4–activating peptide (PAR4-AP), which induced platelet activation and aggregation, was successful in demonstrating Epithelial Attachment via BL and Epithelial sheet formation on the titanium surface. We hypothesized that the platelet-induced Epithelial sheet on PAR4-AP–modified titanium surfaces would reduce bacterial Attachment, penetration, and invasion. Titanium surface was modified with PAR4-AP and incubated with platelet-r...
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establishment of Epithelial Attachment on titanium surface coated with platelet activating peptide
PLOS ONE, 2016Co-Authors: Masazumi Nagai, Shigemi Nagai, Shiho Sugawara, Masahiko Maeno, John D Da Silva, Hisatomo KondoAbstract:The aim of this study was to produce Epithelial Attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of Epithelial sealing at the peri-implant collar. Histologically, Epithelial sealing is recognized as the Attachment of the basement membrane (BM). BM-Attachment is promoted by activated platelet aggregates at surgical wound sites. On the other hand, platelets did not aggregate on smooth titanium, the surface typical of the implant abutment. We then hypothesized that Epithelial BM-Attachment was produced when titanium surface was modified to allow platelet aggregation. Titanium surfaces were coated with a protease activated receptor 4-activating peptide (PAR4-AP). PAR4-AP coating yielded rapid aggregation of platelets on the titanium surface. Platelet aggregates released robust amount of Epithelial chemoattractants (IGF-I, TGF-β) and growth factors (EGF, VEGF) on the titanium surface. Human gingival Epithelial cells, when they were co-cultured on the platelet aggregates, successfully attached to the PAR4-AP coated titanium surface with spread laminin5 positive BM and consecutive staining of the Epithelial tight junction component ZO1, indicating the formation of complete Epithelial sheet. These in-vitro results indicate the establishment of Epithelial BM-Attachment to the titanium surface.
Hisatomo Kondo - One of the best experts on this subject based on the ideXlab platform.
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Epithelial cell adhesion efficacy of a novel peptide identified by panning on a smooth titanium surface
International Journal of Oral Science, 2018Co-Authors: Hidemichi Kihara, David M. Kim, Masazumi Nagai, Toshiki Nojiri, Shigemi Nagai, Cliff Lee, Wataru Hatakeyama, Chia-yu Chen, Hisatomo Kondo, John SilvaAbstract:Epithelial Attachment via the basal lamina on the tooth surface provides an important structural defence mechanism against bacterial invasion in combating periodontal disease. However, when considering dental implants, strong Epithelial Attachment does not exist throughout the titanium-soft tissue interface, making soft tissues more susceptible to peri-implant disease. This study introduced a novel synthetic peptide (A10) to enhance Epithelial Attachment. A10 was identified from a bacterial peptide display library and synthesized. A10 and protease-activated receptor 4-activating peptide (PAR4-AP, positive control) were immobilized on commercially pure titanium. The peptide-treated titanium showed high Epithelial cell migration ability during incubation in platelet-rich plasma. We confirmed the development of dense and expanded BL (stained by Ln5) with pericellular junctions (stained by ZO1) on the peptide-treated titanium surface. In an adhesion assay of Epithelial cells on A10-treated titanium, PAR4-AP-treated titanium, bovine root and non-treated titanium, A10-treated titanium and PAR4-AP-treated titanium showed significantly stronger adhesion than non-treated titanium. PAR4-AP-treated titanium showed significantly higher inflammatory cytokine release than non-treated titanium. There was no significant difference in inflammatory cytokine release between A10-treated and non-treated titanium. These results indicated that A10 could induce the adhesion and migration of Epithelial cells with low inflammatory cytokine release. This novel peptide has a potentially useful application that could improve clinical outcomes with titanium implants and abutments by reducing or preventing peri-implant disease.
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establishment of Epithelial Attachment on titanium surface coated with platelet activating peptide
PLOS ONE, 2016Co-Authors: Masazumi Nagai, Shigemi Nagai, Shiho Sugawara, Masahiko Maeno, John D Da Silva, Hisatomo KondoAbstract:The aim of this study was to produce Epithelial Attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of Epithelial sealing at the peri-implant collar. Histologically, Epithelial sealing is recognized as the Attachment of the basement membrane (BM). BM-Attachment is promoted by activated platelet aggregates at surgical wound sites. On the other hand, platelets did not aggregate on smooth titanium, the surface typical of the implant abutment. We then hypothesized that Epithelial BM-Attachment was produced when titanium surface was modified to allow platelet aggregation. Titanium surfaces were coated with a protease activated receptor 4-activating peptide (PAR4-AP). PAR4-AP coating yielded rapid aggregation of platelets on the titanium surface. Platelet aggregates released robust amount of Epithelial chemoattractants (IGF-I, TGF-β) and growth factors (EGF, VEGF) on the titanium surface. Human gingival Epithelial cells, when they were co-cultured on the platelet aggregates, successfully attached to the PAR4-AP coated titanium surface with spread laminin5 positive BM and consecutive staining of the Epithelial tight junction component ZO1, indicating the formation of complete Epithelial sheet. These in-vitro results indicate the establishment of Epithelial BM-Attachment to the titanium surface.
Shiho Sugawara - One of the best experts on this subject based on the ideXlab platform.
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establishment of Epithelial Attachment on titanium surface coated with platelet activating peptide
PLOS ONE, 2016Co-Authors: Masazumi Nagai, Shigemi Nagai, Shiho Sugawara, Masahiko Maeno, John D Da Silva, Hisatomo KondoAbstract:The aim of this study was to produce Epithelial Attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of Epithelial sealing at the peri-implant collar. Histologically, Epithelial sealing is recognized as the Attachment of the basement membrane (BM). BM-Attachment is promoted by activated platelet aggregates at surgical wound sites. On the other hand, platelets did not aggregate on smooth titanium, the surface typical of the implant abutment. We then hypothesized that Epithelial BM-Attachment was produced when titanium surface was modified to allow platelet aggregation. Titanium surfaces were coated with a protease activated receptor 4-activating peptide (PAR4-AP). PAR4-AP coating yielded rapid aggregation of platelets on the titanium surface. Platelet aggregates released robust amount of Epithelial chemoattractants (IGF-I, TGF-β) and growth factors (EGF, VEGF) on the titanium surface. Human gingival Epithelial cells, when they were co-cultured on the platelet aggregates, successfully attached to the PAR4-AP coated titanium surface with spread laminin5 positive BM and consecutive staining of the Epithelial tight junction component ZO1, indicating the formation of complete Epithelial sheet. These in-vitro results indicate the establishment of Epithelial BM-Attachment to the titanium surface.
Hidemichi Kihara - One of the best experts on this subject based on the ideXlab platform.
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Epithelial cell adhesion efficacy of a novel peptide identified by panning on a smooth titanium surface
International Journal of Oral Science, 2018Co-Authors: Hidemichi Kihara, David M. Kim, Masazumi Nagai, Toshiki Nojiri, Shigemi Nagai, Cliff Lee, Wataru Hatakeyama, Chia-yu Chen, Hisatomo Kondo, John SilvaAbstract:Epithelial Attachment via the basal lamina on the tooth surface provides an important structural defence mechanism against bacterial invasion in combating periodontal disease. However, when considering dental implants, strong Epithelial Attachment does not exist throughout the titanium-soft tissue interface, making soft tissues more susceptible to peri-implant disease. This study introduced a novel synthetic peptide (A10) to enhance Epithelial Attachment. A10 was identified from a bacterial peptide display library and synthesized. A10 and protease-activated receptor 4-activating peptide (PAR4-AP, positive control) were immobilized on commercially pure titanium. The peptide-treated titanium showed high Epithelial cell migration ability during incubation in platelet-rich plasma. We confirmed the development of dense and expanded BL (stained by Ln5) with pericellular junctions (stained by ZO1) on the peptide-treated titanium surface. In an adhesion assay of Epithelial cells on A10-treated titanium, PAR4-AP-treated titanium, bovine root and non-treated titanium, A10-treated titanium and PAR4-AP-treated titanium showed significantly stronger adhesion than non-treated titanium. PAR4-AP-treated titanium showed significantly higher inflammatory cytokine release than non-treated titanium. There was no significant difference in inflammatory cytokine release between A10-treated and non-treated titanium. These results indicated that A10 could induce the adhesion and migration of Epithelial cells with low inflammatory cytokine release. This novel peptide has a potentially useful application that could improve clinical outcomes with titanium implants and abutments by reducing or preventing peri-implant disease.
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function of platelet induced Epithelial Attachment at titanium surfaces inhibits microbial colonization
Journal of Dental Research, 2017Co-Authors: M Maeno, Hidemichi Kihara, Shigemi Nagai, J D Da Silva, S Sugawara, Y Nara, Masazumi NagaiAbstract:The aim of this study was to evaluate the barrier function of platelet-induced Epithelial sheets on titanium surfaces. The lack of functional peri-implant Epithelial sealing with basal lamina (BL) Attachment at the interface of the implant and the adjacent epithelium allows for bacterial invasion, which may lead to peri-implantitis. Although various approaches have been reported to combat bacterial infection by surface modifications to titanium, none of these have been successful in a clinical application. In our previous study, surface modification with protease-activated receptor 4–activating peptide (PAR4-AP), which induced platelet activation and aggregation, was successful in demonstrating Epithelial Attachment via BL and Epithelial sheet formation on the titanium surface. We hypothesized that the platelet-induced Epithelial sheet on PAR4-AP–modified titanium surfaces would reduce bacterial Attachment, penetration, and invasion. Titanium surface was modified with PAR4-AP and incubated with platelet-r...