The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
Wei-li Chen - One of the best experts on this subject based on the ideXlab platform.
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the effect of topical autologous serum on graft re Epithelialization after penetrating keratoplasty
American Journal of Ophthalmology, 2010Co-Authors: Yan-ming Chen, Jehn-yu Huang, Elizabeth P. Shen, Tzu-yun Tsai, Wei-li ChenAbstract:● PURPOSE: To analyze factors influencing corneal graft re-Epithelialization after penetrating keratoplasty (PK) and evaluate the effect of topical autologous serum in promoting graft re-Epithelialization. ● DESIGN: Prospective interventional study. ● METHODS: We analyzed 165 eyes of 165 patients who underwent PK between January 1, 2005 and December 31, 2007. Patients were divided into 2 groups according to routine use or non-use of postoperative 20% topical autologous serum. Postoperative slit-lamp examination after fluorescein staining was performed, and graft reEpithelialization time was recorded. Recipient/donor characteristics, surgical variables, and topical use of autologous serum were analyzed for their effects on post-PK graft re-Epithelialization. Statistical analysis was performed by univariate and multivariate regression analysis using the ordinal logistic fit model to assess the potential risk factors influencing graft re-Epithelialization after PK. ● RESULTS: In univariate analysis, diabetes mellitus (DM), longer death-to-storage time and death-to-surgery time of the donor, and larger recipient size significantly delayed graft re-Epithelialization (P < .05). Use of autologous serum significantly expedited graft re-Epithelialization (P .004). In multiple regression analysis, only DM in the recipient (odds ratio [OR] 5.10, P < .001), postoperative use of autologous serum (OR 0.54, P .046), and larger graft size (OR 4.44, P < .001) influenced graft re-Epithelialization. The beneficial and healing effect of autologous serum is particularly significant in diabetic recipients and larger grafts. ● CONCLUSIONS: Several factors may influence graft re-Epithelialization after PK. Graft re-Epithelialization time was longer in diabetic recipients and larger grafts. Use of autologous serum may be a beneficial strategy in these patients with potentially delayed epithelial healing.
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The effect of topical autologous serum on graft re-Epithelialization after penetrating keratoplasty.
American journal of ophthalmology, 2010Co-Authors: Yan-ming Chen, Jehn-yu Huang, Elizabeth P. Shen, Tzu-yun Tsai, Wei-li ChenAbstract:To analyze factors influencing corneal graft re-Epithelialization after penetrating keratoplasty (PK) and evaluate the effect of topical autologous serum in promoting graft re-Epithelialization. Prospective interventional study. We analyzed 165 eyes of 165 patients who underwent PK between January 1, 2005 and December 31, 2007. Patients were divided into 2 groups according to routine use or non-use of postoperative 20% topical autologous serum. Postoperative slit-lamp examination after fluorescein staining was performed, and graft re-Epithelialization time was recorded. Recipient/donor characteristics, surgical variables, and topical use of autologous serum were analyzed for their effects on post-PK graft re-Epithelialization. Statistical analysis was performed by univariate and multivariate regression analysis using the ordinal logistic fit model to assess the potential risk factors influencing graft re-Epithelialization after PK. In univariate analysis, diabetes mellitus (DM), longer death-to-storage time and death-to-surgery time of the donor, and larger recipient size significantly delayed graft re-Epithelialization (P < .05). Use of autologous serum significantly expedited graft re-Epithelialization (P = .004). In multiple regression analysis, only DM in the recipient (odds ratio [OR] = 5.10, P < .001), postoperative use of autologous serum (OR = 0.54, P = .046), and larger graft size (OR = 4.44, P < .001) influenced graft re-Epithelialization. The beneficial and healing effect of autologous serum is particularly significant in diabetic recipients and larger grafts. Several factors may influence graft re-Epithelialization after PK. Graft re-Epithelialization time was longer in diabetic recipients and larger grafts. Use of autologous serum may be a beneficial strategy in these patients with potentially delayed epithelial healing. Copyright (c) 2010 Elsevier Inc. All rights reserved.
Yan-ming Chen - One of the best experts on this subject based on the ideXlab platform.
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the effect of topical autologous serum on graft re Epithelialization after penetrating keratoplasty
American Journal of Ophthalmology, 2010Co-Authors: Yan-ming Chen, Jehn-yu Huang, Elizabeth P. Shen, Tzu-yun Tsai, Wei-li ChenAbstract:● PURPOSE: To analyze factors influencing corneal graft re-Epithelialization after penetrating keratoplasty (PK) and evaluate the effect of topical autologous serum in promoting graft re-Epithelialization. ● DESIGN: Prospective interventional study. ● METHODS: We analyzed 165 eyes of 165 patients who underwent PK between January 1, 2005 and December 31, 2007. Patients were divided into 2 groups according to routine use or non-use of postoperative 20% topical autologous serum. Postoperative slit-lamp examination after fluorescein staining was performed, and graft reEpithelialization time was recorded. Recipient/donor characteristics, surgical variables, and topical use of autologous serum were analyzed for their effects on post-PK graft re-Epithelialization. Statistical analysis was performed by univariate and multivariate regression analysis using the ordinal logistic fit model to assess the potential risk factors influencing graft re-Epithelialization after PK. ● RESULTS: In univariate analysis, diabetes mellitus (DM), longer death-to-storage time and death-to-surgery time of the donor, and larger recipient size significantly delayed graft re-Epithelialization (P < .05). Use of autologous serum significantly expedited graft re-Epithelialization (P .004). In multiple regression analysis, only DM in the recipient (odds ratio [OR] 5.10, P < .001), postoperative use of autologous serum (OR 0.54, P .046), and larger graft size (OR 4.44, P < .001) influenced graft re-Epithelialization. The beneficial and healing effect of autologous serum is particularly significant in diabetic recipients and larger grafts. ● CONCLUSIONS: Several factors may influence graft re-Epithelialization after PK. Graft re-Epithelialization time was longer in diabetic recipients and larger grafts. Use of autologous serum may be a beneficial strategy in these patients with potentially delayed epithelial healing.
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The effect of topical autologous serum on graft re-Epithelialization after penetrating keratoplasty.
American journal of ophthalmology, 2010Co-Authors: Yan-ming Chen, Jehn-yu Huang, Elizabeth P. Shen, Tzu-yun Tsai, Wei-li ChenAbstract:To analyze factors influencing corneal graft re-Epithelialization after penetrating keratoplasty (PK) and evaluate the effect of topical autologous serum in promoting graft re-Epithelialization. Prospective interventional study. We analyzed 165 eyes of 165 patients who underwent PK between January 1, 2005 and December 31, 2007. Patients were divided into 2 groups according to routine use or non-use of postoperative 20% topical autologous serum. Postoperative slit-lamp examination after fluorescein staining was performed, and graft re-Epithelialization time was recorded. Recipient/donor characteristics, surgical variables, and topical use of autologous serum were analyzed for their effects on post-PK graft re-Epithelialization. Statistical analysis was performed by univariate and multivariate regression analysis using the ordinal logistic fit model to assess the potential risk factors influencing graft re-Epithelialization after PK. In univariate analysis, diabetes mellitus (DM), longer death-to-storage time and death-to-surgery time of the donor, and larger recipient size significantly delayed graft re-Epithelialization (P < .05). Use of autologous serum significantly expedited graft re-Epithelialization (P = .004). In multiple regression analysis, only DM in the recipient (odds ratio [OR] = 5.10, P < .001), postoperative use of autologous serum (OR = 0.54, P = .046), and larger graft size (OR = 4.44, P < .001) influenced graft re-Epithelialization. The beneficial and healing effect of autologous serum is particularly significant in diabetic recipients and larger grafts. Several factors may influence graft re-Epithelialization after PK. Graft re-Epithelialization time was longer in diabetic recipients and larger grafts. Use of autologous serum may be a beneficial strategy in these patients with potentially delayed epithelial healing. Copyright (c) 2010 Elsevier Inc. All rights reserved.
Esko Kankuri - One of the best experts on this subject based on the ideXlab platform.
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improved skin wound Epithelialization by topical delivery of soluble factors from fibroblast aggregates
Burns, 2012Co-Authors: Matti Peura, Ilkka Kaartinen, Sari Suomela, Mika Hukkanen, Jozef Bizik, Ari Harjula, Esko KankuriAbstract:Abstract Introduction Timely coverage of an excised burn wound with a split-thickness skin graft, and efficient Epithelialization at the donor site wound are key components in the treatment of burn patients. Prompt healing is dependent on paracrine support from underlying dermal connective tissue fibroblasts. Study aim Using the skin graft donor site in pig as a model for Epithelialization, our aim was to evaluate if dermal signals, derived from cultured dermal fibroblast aggregates (Finectra), can promote epidermal regeneration. Materials and methods Partial-thickness skin wounds were made with a dermatome on the backs of three domestic pigs. After randomization, topical treatment was initiated by application of Finectra ( n = 6) or factors from standard fibroblast monolayer cultures ( n = 6) trapped in a slow-clotting fibrin matrix. Saline was applied to contralateral wounds to serve as corresponding untreated controls ( n = 12). After 3 days, full-thickness skin samples representing the whole wound area were obtained. Histological sections of these samples were analyzed for Epithelialization, cell migration from lateral wound edges and hair follicles, as well as for formation of granulation tissue. Results In response to topical delivery of Finectra, a significant acceleration of Epithelialization ( p p Conclusion These data show that the fibroblast aggregate-derived paracrine mediators, Finectra, stimulate epidermal regeneration in vivo .
Gordon R. Tobin - One of the best experts on this subject based on the ideXlab platform.
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Ketanserin accelerates wound Epithelialization and neovascularization.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 1995Co-Authors: Michael Kim, Dorthe Kjølseth, Gordon R. Tobin, Dale A. Schuschke, E. Tuncay Ustuner, Anni Morsing, Victor H. Fingar, Jeffrey Wieman, M Kamler, Sheldon J. BondAbstract:We investigated the acute effects of topical ketanserin, a 5-HT(2) (serotonin) receptor blocker, on wound Epithelialization and vascularization with the use of the hairless mouse ear model. Varying concentrations of Ketanserin (0%, 0.2%, 2.0%, 20% weight/volume) were administered to standardized full-thickness skin wounds on the dorsum of the hairless mouse ear immediately after surgery and daily thereafter. With the use of video microscopy and computer-assisted planimetry, vascularization and Epithelialization were traced every third day until the wounds were fully healed. Arteriole diameters at selected sites near the skin wound were measured before wound creation and after wounding. It was concluded that topically administered ketanserin significantly accelerates both the vascular (p < 0.001 at 2% and 20% concentrations) and epithelial (p < 0.001 at 20% concentration) rates of wound healing in full-thickness nonpathologic skin wounds. Vasodilation of terminal arterioles was not a major response to Ketanserin. Faster Epithelialization was possibly due to direct effect of ketanserin on epithelial cells.
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Comparison of the effects of commonly used wound agents on Epithelialization and neovascularization.
Journal of The American College of Surgeons, 1994Co-Authors: Dorthe Kjølseth, Rosenthal Ai, Campbell Fr, Gordon R. Tobin, Gary L Anderson, John H Barker, Dale A. Schuschke, Robert D. Acland, Johannes Frank, Leonard J. WeinerAbstract:BACKGROUND: The primary effect sought with most topical wound therapy is antimicrobial. Topical wound agents are thought to promote normal healing by protecting the wound from infection. In this study, we examined the effect of six commonly used topical wound agents (bacitracin, sodium hypochlorite, silver nitrate, silver sulfadiazine, mafenide acetate, and povidone-iodine) on Epithelialization and neovascularization in noninfected wounds. For this study, a new wound model was used in which direct visualization and quantification of wound Epithelialization and neovascularization were carried out throughout the entire healing process. STUDY DESIGN: We measured the effect which 500 U per g of bacitracin, 0.25 percent of sodium hypochlorite, 0.5 percent silver nitrate, 1 percent silver sulfadiazine, 8.5 percent mafenide acetate, and 10 percent povodione-iodine had on the rate of wound Epithelialization and neovascularization. The agents were applied topically to 99 circular full-thickness wounds (2.25 mm diameter, 0.125 mm depth) created on the dorsum of male hairless mouse ears. This model enabled us to visualize and measure directly wound Epithelialization and neovascularization repeatedly throughout healing, using intravital video microscopy and computerized digitized planimetry. RESULTS: Control wounds and wounds treated with silver sulfadiazine (n = 18) and mafenide acetate (n = 14) epithelialized in 7.2 +/- 0.7, 7.1 +/- 0.3, and 7.3 +/- 0.3 days, respectively. This was significantly (p < 0.01) faster than the wounds treated with povidone-iodine (n = 10), sodium hypochlorite, (n = 8), and bacitracin (n = 13). Wounds treated with povidone-iodine epithelialized the slowest (11.8 +/- 0.55 days). Wound neovascularization was completed most rapidly in the groups treated with povidone-iodine and silver sulfadiazine (15.0 +/- 0.4 and 15.3 +/- 0.7 days, respectively). This was significantly (p < 0.05) faster than wounds treated with silver nitrate (n = 15), which neovascularized in 18.4 +/- 0.56 days. One-half of the wounds treated with sodium hypochlorite (eight of 16) did not epithelialize or neovascularize. CONCLUSIONS: The various antimicrobial agents studied in our in vivo model affect wound Epithelialization and neovascularization differently. These effects on these two very important aspects of healing should be taken into consideration when indicating a specific agent for treatment of different types of wounds.
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Comparison of the effects of commonly used wound agents on Epithelialization and neovascularization.
Journal of the American College of Surgeons, 1994Co-Authors: Kjolseth D, Gary L Anderson, John H Barker, Robert D. Acland, Schuschke D, Gordon R. TobinAbstract:The primary effect sought with most topical wound therapy is antimicrobial. Topical wound agents are thought to promote normal healing by protecting the wound from infection. In this study, we examined the effect of six commonly used topical wound agents (bacitracin, sodium hypochlorite, silver nitrate, silver sulfadiazine, mafenide acetate, and povidone-iodine) on Epithelialization and neovascularization in noninfected wounds. For this study, a new wound model was used in which direct visualization and quantification of wound Epithelialization and neovascularization were carried out throughout the entire healing process. We measured the effect which 500 U per g of bacitracin, 0.25 percent of sodium hypochlorite, 0.5 percent silver nitrate, 1 percent silver sulfadiazine, 8.5 percent mafenide acetate, and 10 percent povodione-iodine had on the rate of wound Epithelialization and neovascularization. The agents were applied topically to 99 circular full-thickness wounds (2.25 mm diameter, 0.125 mm depth) created on the dorsum of male hairless mouse ears. This model enabled us to visualize and measure directly wound Epithelialization and neovascularization repeatedly throughout healing, using intravital video microscopy and computerized digitized planimetry. Control wounds and wounds treated with silver sulfadiazine (n = 18) and mafenide acetate (n = 14) epithelialized in 7.2 +/- 0.7, 7.1 +/- 0.3, and 7.3 +/- 0.3 days, respectively. This was significantly (p < 0.01) faster than the wounds treated with povidone-iodine (n = 10), sodium hypochlorite, (n = 8), and bacitracin (n = 13). Wounds treated with povidone-iodine epithelialized the slowest (11.8 +/- 0.55 days). Wound neovascularization was completed most rapidly in the groups treated with povidone-iodine and silver sulfadiazine (15.0 +/- 0.4 and 15.3 +/- 0.7 days, respectively). This was significantly (p < 0.05) faster than wounds treated with silver nitrate (n = 15), which neovascularized in 18.4 +/- 0.56 days. One-half of the wounds treated with sodium hypochlorite (eight of 16) did not epithelialize or neovascularize. The various antimicrobial agents studied in our in vivo model affect wound Epithelialization and neovascularization differently. These effects on these two very important aspects of healing should be taken into consideration when indicating a specific agent for treatment of different types of wounds.
W. R. Schouten - One of the best experts on this subject based on the ideXlab platform.
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identification of Epithelialization in high transsphincteric fistulas
Techniques in Coloproctology, 2012Co-Authors: Litza E. Mitalas, R. S. Van Onkelen, K. Monkhorst, David D. E. Zimmerman, Martijn P. Gosselink, W. R. SchoutenAbstract:Background At present, transanal advancement flap repair (TAFR) is the treatment of choice for transsphincteric fistulas passing through the upper and middle third of the external anal sphincter. It has been suggested that Epithelialization of the fistula tract contributes to the failure of the treatment. The aim of this study was to assess the prevalence of Epithelialization of the fistula tract and to study its effect on the outcome of TAFR and TAFR combined with ligation of the intersphincteric fistula tract (LIFT).
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Identification of Epithelialization in high transsphincteric fistulas.
Techniques in coloproctology, 2012Co-Authors: Litza E. Mitalas, R. S. Van Onkelen, K. Monkhorst, David D. E. Zimmerman, Martijn P. Gosselink, W. R. SchoutenAbstract:At present, transanal advancement flap repair (TAFR) is the treatment of choice for transsphincteric fistulas passing through the upper and middle third of the external anal sphincter. It has been suggested that Epithelialization of the fistula tract contributes to the failure of the treatment. The aim of this study was to assess the prevalence of Epithelialization of the fistula tract and to study its effect on the outcome of TAFR and TAFR combined with ligation of the intersphincteric fistula tract (LIFT). Forty-four patients with a high transsphincteric fistula of cryptoglandular origin underwent TAFR. Nine of these patients underwent a combined procedure of TAFR with LIFT. In all patients the fistula tract was excised from the external opening up to the outer border of the external anal sphincter. In patients undergoing TAFR combined with LIFT an additional central part of the intersphincteric fistula tract was excised. A total of 53 specimens were submitted. Histopathological examination of the specimens was carried out by a pathologist, blinded for clinical data. Epithelialization of the distal and intersphincteric fistula tract was observed in only 25 and 22% of fistulas, respectively. There was no difference in outcome between fistulas with or without Epithelialization. Epithelialization of high transsphincteric fistulas is rare and does not affect the outcome of TAFR and TAFR combined with LIFT. © The Author(s) 2012. This article is published with open access at Springerlink.com
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Identification of Epithelialization in high transsphincteric fistulas L. E. MitalasR. S. van OnkelenK. Monkhorst
2012Co-Authors: W. R. SchoutenAbstract:Background At present, transanal advancement flap repair (TAFR) is the treatment of choice for transsphincteric fistulas passing through the upper and middle third of the external anal sphincter. It has been suggested that Epithelialization of the fistula tract contributes to the failure of the treatment. The aim of this study was to assess the prevalence of Epithelialization of the fistula tract and to study its effect on the outcome of TAFR and TAFR combined with ligation of the intersphincteric fistula tract (LIFT). Methods Forty-four patients with a high transsphincteric fistula of cryptoglandular origin underwent TAFR. Nine of these patients underwent a combined procedure of TAFR with LIFT. In all patients the fistula tract was excised from the external opening up to the outer border of the external anal sphincter. In patients undergoing TAFR combined with LIFT an additional central part of the intersphincteric fistula tract was excised. A total of 53 specimens were submitted. Histopathological examination of the specimens was carried out by a pathologist, blinded for clinical data. Results Epithelialization of the distal and intersphincteric fistula tract was observed in only 25 and 22% of fistulas, respectively. There was no difference in outcome between fistulas with or without Epithelialization. Conclusions Epithelialization of high transsphincteric fistulas is rare and does not affect the outcome of TAFR and TAFR combined with LIFT.