The Experts below are selected from a list of 78 Experts worldwide ranked by ideXlab platform
I O Ellis - One of the best experts on this subject based on the ideXlab platform.
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infiltrating Epitheliosis of the breast characterization of histological features immunophenotype and genomic profile
Histopathology, 2016Co-Authors: Carey A Eberle, Salvatore Piscuoglio, Emad A Rakha, Felipe C Geyer, Marcia Edelweiss, Rita A Sakr, Britta Weigelt, Jorge S Reisfilho, I O EllisAbstract:Aims Infiltrating Epitheliosis is a rare complex sclerosing lesion (CSL) of the breast, characterized by infiltrating ducts immersed in a scleroelastotic stroma and filled with cells having architectural and cytological patterns reminiscent of those of usual ductal hyperplasia. In this study we sought to define the molecular characteristics of infiltrating Epitheliosis. Methods and results Eight infiltrating Epitheliosis, adjacent breast lesions (one usual ductal hyperplasia, one papilloma, one micropapillary ductal carcinoma in situ and one low-grade adenosquamous carcinoma), and corresponding normal breast tissue from each case were microdissected and subjected to massively parallel sequencing analysis targeting all coding regions of 254 genes mutated recurrently in breast cancer and/or related to DNA repair. Mutations in components of the PI3K pathway were found in all infiltrating Epitheliosis samples, seven of which harboured PIK3CA hotspot mutations, while the remaining case displayed a PIK3R1 somatic mutation. Conclusions Somatic mutations affecting PI3K pathway genes were found to be highly prevalent in infiltrating Epitheliosis, suggesting that these lesions may be neoplastic rather than hyperplastic. The landscape of somatic genetic alterations found in infiltrating Epitheliosis is similar to that of radial scars/CSLs, suggesting that infiltrating Epitheliosis may represent one end of this spectrum of lesions.
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infiltrative Epitheliosis of the breast
Journal of Clinical Pathology, 2012Co-Authors: Rin Yamaguchi, Emad A Rakha, I O Ellis, Kanetaka Maeshiro, Jun Taguchi, Ichiro Shima, Hideki Kamei, Minoru Suzuki, Mahiro Imamura, Gouichi NakayamaAbstract:A 77-year-old woman noted a mass in her right breast, following which she presented to the Asakura Medical Association Hospital (Asakura, Japan). The mass was soft, smooth and round, without lymph node swelling of the axilla, and ∼2.0 cm in size. Her CA19-9 level was slightly high at 39.2 U/ml (normal: <37.0 U/ml). She was involved in a traffic accident 30 years ago. On imaging, mammography showed a regularly shaped mass, while ultrasonography indicated a combination mass with a solid and cystic pattern measuring 2.3×1.1 cm (figure 1). The tumour was therefore considered to be generally benign but solid and had a cystic pattern admixture with unusual ultrasonographic findings. Since malignancy could not be completely excluded, and in accordance with the patient's request, a needle biopsy was performed. Figure 1 Ultrasonographic findings in the right breast. Ultrasonography demonstrated a tumour with a solid portion and cystic lesions. The tumour showed a heterogeneous echo level with anechoic cystic areas. The biopsy specimen showed papillary lesions with abundant stromal proliferations. Under low-power magnification, the nodular lesion appeared to be invasive. On the other hand, under high-power magnification, the luminal cells were cytologically bland and appeared jumbled on top of each other, and some parts indicated possible squamous differentiation. Since there was a suspicion of an invasive process having occurred in the tissue, an additional specimen was requested and an open biopsy was performed. In the follow-up open biopsy specimen, the mass lesion was vaguely nodular with irregular glands together …
B Katzer - One of the best experts on this subject based on the ideXlab platform.
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histopathology of myoepithelial basocellular hyperplasias in adenosis and Epitheliosis of the breast demonstrated by the reactivity of cytokeratins and s100 protein an analysis of heterogenic cell proliferations in 90 cases of benign and malignant breast diseases
Virchows Archiv, 1992Co-Authors: R Bassler, B KatzerAbstract:This study on the different types of epithelial hyperplasia in fibrocystic disease was inspired by the observation of myoepithelial (basocellular) hyperplasia identified by strong expression of S100 protein and a weak reaction with antibodies against cytokeratin (KL1) in cells forming solid and acinar buds. The cells do not contain immunohistochemically detectable actin or desmin. Glandular transformation and proliferation give rise to basocellular circumductal adenosis. Normal breast tissue, 51 cases of fibrocystic disease with mild, florid and atypical hyperplasias, 7 fibroadenomas and 20 cases of carcinoma in situ were studied and a semiquantitative analysis revealed basal buds and adenosis in less than 40% of cases of mild hyperplasia and up to 73% in florid hyperplasia. Epitheliosis is characterized by a heterogeneous cell pattern with cells positive for S100 protein in 30-60%, but in small ducts up to 100% with an immediate connection to the basal cell layer were positive. Carcinoma in situ contained very rare tumour cells positive for S100 protein. The cells expressing S100 protein in terminal ducts, in adenosis and Epitheliosis showed only some of the characteristics of myoepithelial cells, since they lack immunoreactivity with antibodies against actin. These basal clear cells are interpreted as transitional or indeterminate cells with features of myoepithelial precursor cells, but with the ability to develop basocellular nodular and glandular hyperplasia in the ductulo-lobular units in cases of adenosis and juvenile fibroadenoma.
John K C Chan - One of the best experts on this subject based on the ideXlab platform.
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endocrine ductal carcinoma in situ e dcis of the breast a form of low grade dcis with distinctive clinicopathologic and biologic characteristics
The American Journal of Surgical Pathology, 1996Co-Authors: William Y W Tsang, John K C ChanAbstract:Abstract Endocrine ductal carcinoma in situ (E-DCIS), first characterized by Cross et al. in 1985, is an uncommon entity, and there is little information on its pathobiologic features and natural history in the literature. This report describes the largest series of 34 cases: 14 cases were pure in situ (group A), and 20 were accompanied by an invasive component (group B). All except three patients were over the age of 60 years, with the mean being 69.5 years for group A and 72.6 years for group B. Except for six patients in group A who had nipple discharge, all had a breast mass. On follow-up, one of five group A patients developed local recurrence 5 years after mastectomy, and two of seven group B patients developed another invasive primary in the contralateral breast. Histologically, E-DCIS showed expansile intraductal growths forming solid sheets and festoons traversed by delicate fibrovascular septa. Accumulation of basophilic mucin might be found within the growth and the fibrovascular septa. There were variable degrees of stromal sclerosis. In some cases, the solid intraductal cellular proliferations were focally punctuated by microglandular spaces and rosettes. Comedo necrosis was absent. Intraductal papillomas were found in the immediate vicinity of the tumors in 18 cases and invariably showed pagetoid involvement by E-DCIS. Pagetoid spread into the adjacent ducts and ductules was also a common feature (17 cases). The tumor cells were polygonal, oval, or spindly, often with eccentrically placed, bland-looking, ovoid nuclei and abundant eosinophilic granular cytoplasm. Intracellular mucin was commonly demonstrable. Immunostaining for myoepithelium using muscle-specific actin antibody confirmed the in situ nature of the E-DCIS component. The majority of tumor cells showed strong staining with the neuroendocrine markers chromogranin, synaptophysin, and neuron-specific enolase (monoclonal). Immunostaining also dramatically highlighted the pagetoid spread into the papillomas and ductules by outlining the tumor cells between the negatively stained residual ductal epithelium and myoepithelium. All cases were immunoreactive for estrogen and progesterone receptor, but not p53 and c-erbB2. The Ki-67 index was < 5%. Ultrastructural studies on four cases showed many dense-core neurosecretory granules and larger mucigen granules. In group B cases, the invasive component, which comprised 5-95% of the tumor, included colloid carcinoma, 12; "carcinoid" tumor, 3; mixed "carcinoid"/colloid carcinoma, 4; and small cell neuroendocrine carcinoma, 1. Neuroendocrine markers were also consistently demonstrable in the invasive component. In conclusion, E-DCIS is predominantly a disease of older women that is frequently accompanied by papillomas in the vicinity and may present as nipple discharge (an uncommon presentation in the usual forms of DCIS). It can mimic Epitheliosis histologically, but the pagetoid spread is a helpful clue to its neoplastic nature. The bland nuclear morphology, lack of necrosis, and biologic marker profile suggest that E-DCIS is a form of low-grade DCIS despite its solid growth pattern. The invasive carcinomas associated with E-DCIS are also neuroendocrine programmed rather than the usual types of ductal carcinomas, suggesting that E-DCIS represents a biologically distinctive category of DCIS.
Carey A Eberle - One of the best experts on this subject based on the ideXlab platform.
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infiltrating Epitheliosis of the breast characterization of histological features immunophenotype and genomic profile
Histopathology, 2016Co-Authors: Carey A Eberle, Salvatore Piscuoglio, Emad A Rakha, Felipe C Geyer, Marcia Edelweiss, Rita A Sakr, Britta Weigelt, Jorge S Reisfilho, I O EllisAbstract:Aims Infiltrating Epitheliosis is a rare complex sclerosing lesion (CSL) of the breast, characterized by infiltrating ducts immersed in a scleroelastotic stroma and filled with cells having architectural and cytological patterns reminiscent of those of usual ductal hyperplasia. In this study we sought to define the molecular characteristics of infiltrating Epitheliosis. Methods and results Eight infiltrating Epitheliosis, adjacent breast lesions (one usual ductal hyperplasia, one papilloma, one micropapillary ductal carcinoma in situ and one low-grade adenosquamous carcinoma), and corresponding normal breast tissue from each case were microdissected and subjected to massively parallel sequencing analysis targeting all coding regions of 254 genes mutated recurrently in breast cancer and/or related to DNA repair. Mutations in components of the PI3K pathway were found in all infiltrating Epitheliosis samples, seven of which harboured PIK3CA hotspot mutations, while the remaining case displayed a PIK3R1 somatic mutation. Conclusions Somatic mutations affecting PI3K pathway genes were found to be highly prevalent in infiltrating Epitheliosis, suggesting that these lesions may be neoplastic rather than hyperplastic. The landscape of somatic genetic alterations found in infiltrating Epitheliosis is similar to that of radial scars/CSLs, suggesting that infiltrating Epitheliosis may represent one end of this spectrum of lesions.
Emad A Rakha - One of the best experts on this subject based on the ideXlab platform.
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infiltrating Epitheliosis of the breast characterization of histological features immunophenotype and genomic profile
Histopathology, 2016Co-Authors: Carey A Eberle, Salvatore Piscuoglio, Emad A Rakha, Felipe C Geyer, Marcia Edelweiss, Rita A Sakr, Britta Weigelt, Jorge S Reisfilho, I O EllisAbstract:Aims Infiltrating Epitheliosis is a rare complex sclerosing lesion (CSL) of the breast, characterized by infiltrating ducts immersed in a scleroelastotic stroma and filled with cells having architectural and cytological patterns reminiscent of those of usual ductal hyperplasia. In this study we sought to define the molecular characteristics of infiltrating Epitheliosis. Methods and results Eight infiltrating Epitheliosis, adjacent breast lesions (one usual ductal hyperplasia, one papilloma, one micropapillary ductal carcinoma in situ and one low-grade adenosquamous carcinoma), and corresponding normal breast tissue from each case were microdissected and subjected to massively parallel sequencing analysis targeting all coding regions of 254 genes mutated recurrently in breast cancer and/or related to DNA repair. Mutations in components of the PI3K pathway were found in all infiltrating Epitheliosis samples, seven of which harboured PIK3CA hotspot mutations, while the remaining case displayed a PIK3R1 somatic mutation. Conclusions Somatic mutations affecting PI3K pathway genes were found to be highly prevalent in infiltrating Epitheliosis, suggesting that these lesions may be neoplastic rather than hyperplastic. The landscape of somatic genetic alterations found in infiltrating Epitheliosis is similar to that of radial scars/CSLs, suggesting that infiltrating Epitheliosis may represent one end of this spectrum of lesions.
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infiltrative Epitheliosis of the breast
Journal of Clinical Pathology, 2012Co-Authors: Rin Yamaguchi, Emad A Rakha, I O Ellis, Kanetaka Maeshiro, Jun Taguchi, Ichiro Shima, Hideki Kamei, Minoru Suzuki, Mahiro Imamura, Gouichi NakayamaAbstract:A 77-year-old woman noted a mass in her right breast, following which she presented to the Asakura Medical Association Hospital (Asakura, Japan). The mass was soft, smooth and round, without lymph node swelling of the axilla, and ∼2.0 cm in size. Her CA19-9 level was slightly high at 39.2 U/ml (normal: <37.0 U/ml). She was involved in a traffic accident 30 years ago. On imaging, mammography showed a regularly shaped mass, while ultrasonography indicated a combination mass with a solid and cystic pattern measuring 2.3×1.1 cm (figure 1). The tumour was therefore considered to be generally benign but solid and had a cystic pattern admixture with unusual ultrasonographic findings. Since malignancy could not be completely excluded, and in accordance with the patient's request, a needle biopsy was performed. Figure 1 Ultrasonographic findings in the right breast. Ultrasonography demonstrated a tumour with a solid portion and cystic lesions. The tumour showed a heterogeneous echo level with anechoic cystic areas. The biopsy specimen showed papillary lesions with abundant stromal proliferations. Under low-power magnification, the nodular lesion appeared to be invasive. On the other hand, under high-power magnification, the luminal cells were cytologically bland and appeared jumbled on top of each other, and some parts indicated possible squamous differentiation. Since there was a suspicion of an invasive process having occurred in the tissue, an additional specimen was requested and an open biopsy was performed. In the follow-up open biopsy specimen, the mass lesion was vaguely nodular with irregular glands together …