The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform
Keizo Sugimachi - One of the best experts on this subject based on the ideXlab platform.
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expression of sialyl tn antigens in normal squamous Epithelium Dysplasia and squamous cell carcinoma in the esophagus
Cancer Research, 1993Co-Authors: Yoichi Ikeda, Hiroyuki Kuwano, K Baba, Masahiko Ikebe, Tetuya Matushima, Y Adachi, Masaki Mori, Keizo SugimachiAbstract:Abstract Two monoclonal antibodies, TKH2 and B72.3, directed toward the Sialyl-Tn antigen (SA 2, 6GalNAcα-O-Ser/Thr), were examined immunohistochemically to analyze the expression of these antigens in 20 areas of normal squamous Epithelium, 12 lesions of Dysplasia, and 86 cases of squamous cell carcinoma including 32 with superficial carcinoma in the esophagus. No expression of TKH2 or B72.3 was found in the normal squamous Epithelium. Among the 12 lesions of Dysplasia only one expressed TKH2. In carcinoma the expression of TKH2 and B72.3 was found in 40 (47%) and 21 (24%) of the 86 carcinomas, respectively; however, the number of positive malignant cells with TKH2 and B72.3 totaled less than half that in the tissue, and no relationship was found between either prognosis or lymph node metastasis and the expression of Sialyl-Tn antigen. These results indicate that Sialyl-Tn antigen appears in the process of malignant transformation or tumor progression in esophageal squamous cell carcinoma; however, the positive expression of Sialyl-Tn antigen was not directly connected to either prognosis or lymph node metastasis.
P Gais - One of the best experts on this subject based on the ideXlab platform.
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prediction of the outcome of esophageal Epithelium Dysplasia by high resolution image analysis
Chinese Journal of Cancer Research, 2000Co-Authors: Bin Zhou, Zhen-wei Ding, Jixin Wang, Uta Jutting, Karsten Rodenacker, P GaisAbstract:Objective: To predict the outcome of Dysplasia of esophageal Epithelium by means of high resolution image analysis (HRIA). Methods: Asymptomatic adults were examined for balloon cytology of the esophagus in 1983 from Heshun Commune of Linxian County. 93 severe Dysplasias and 122 mild Dysplasias of the esophagus were selected. By means of an Axiomat-microscope equipped with TV-camera, 100 normal nuclei of well-preserved cells in the intermediate layer of Pap-stained squamous Epithelium were randomly examined. Results: Of the 93 cytologically diagnosed severe Dysplasia cases, 24,14 and 7 progressed to carcinoma in 3, 5 and 9 years, respectively. In the other 48 cases, Dysplasia remained stable or regressed to normal. The other cases were used as the control. According to chromatin features, correct diagnosis of cases was achieved by HRIA in 75.0% (18/24), 85.7% (12/14) and 85.7% (6/7) of the cases examined, respectively (P<0.001). Of the 122 cytologically diagnosed mild Dysplasia, 16,13 and 12 cases progressed to carcinoma in 3, 5 and 9 years, respectively. The other 81 cases remained stable or regressed to normal. Correct diagnosis was made by HRIA in 93.8% (15/16), 76.9% (10/13) and 833% (10/12) of the cases examined, respectively (P<0.001). Conclusion: Chromatin nuclear features examined by HRIA can predict the outcome of precancerous lesions and discriminate progressor from non-progressor ones. It can be used as surrogate endpoint biomarkers for the evaluation of efficiency of chemo-prevention trial.
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Prediction of the outcome of esophageal Epithelium Dysplasia by high resolution image analysis
Chinese Journal of Cancer Research, 2000Co-Authors: Bin Zhou, Zhen-wei Ding, Jixin Wang, Uta Jutting, Karsten Rodenacker, P GaisAbstract:Objective: To predict the outcome of Dysplasia of esophageal Epithelium by means of high resolution image analysis (HRIA). Methods: Asymptomatic adults were examined for balloon cytology of the esophagus in 1983 from Heshun Commune of Linxian County. 93 severe Dysplasias and 122 mild Dysplasias of the esophagus were selected. By means of an Axiomat-microscope equipped with TV-camera, 100 normal nuclei of well-preserved cells in the intermediate layer of Pap-stained squamous Epithelium were randomly examined. Results: Of the 93 cytologically diagnosed severe Dysplasia cases, 24,14 and 7 progressed to carcinoma in 3, 5 and 9 years, respectively. In the other 48 cases, Dysplasia remained stable or regressed to normal. The other cases were used as the control. According to chromatin features, correct diagnosis of cases was achieved by HRIA in 75.0% (18/24), 85.7% (12/14) and 85.7% (6/7) of the cases examined, respectively (P
Yoichi Ikeda - One of the best experts on this subject based on the ideXlab platform.
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expression of sialyl tn antigens in normal squamous Epithelium Dysplasia and squamous cell carcinoma in the esophagus
Cancer Research, 1993Co-Authors: Yoichi Ikeda, Hiroyuki Kuwano, K Baba, Masahiko Ikebe, Tetuya Matushima, Y Adachi, Masaki Mori, Keizo SugimachiAbstract:Abstract Two monoclonal antibodies, TKH2 and B72.3, directed toward the Sialyl-Tn antigen (SA 2, 6GalNAcα-O-Ser/Thr), were examined immunohistochemically to analyze the expression of these antigens in 20 areas of normal squamous Epithelium, 12 lesions of Dysplasia, and 86 cases of squamous cell carcinoma including 32 with superficial carcinoma in the esophagus. No expression of TKH2 or B72.3 was found in the normal squamous Epithelium. Among the 12 lesions of Dysplasia only one expressed TKH2. In carcinoma the expression of TKH2 and B72.3 was found in 40 (47%) and 21 (24%) of the 86 carcinomas, respectively; however, the number of positive malignant cells with TKH2 and B72.3 totaled less than half that in the tissue, and no relationship was found between either prognosis or lymph node metastasis and the expression of Sialyl-Tn antigen. These results indicate that Sialyl-Tn antigen appears in the process of malignant transformation or tumor progression in esophageal squamous cell carcinoma; however, the positive expression of Sialyl-Tn antigen was not directly connected to either prognosis or lymph node metastasis.
Bin Zhou - One of the best experts on this subject based on the ideXlab platform.
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prediction of the outcome of esophageal Epithelium Dysplasia by high resolution image analysis
Chinese Journal of Cancer Research, 2000Co-Authors: Bin Zhou, Zhen-wei Ding, Jixin Wang, Uta Jutting, Karsten Rodenacker, P GaisAbstract:Objective: To predict the outcome of Dysplasia of esophageal Epithelium by means of high resolution image analysis (HRIA). Methods: Asymptomatic adults were examined for balloon cytology of the esophagus in 1983 from Heshun Commune of Linxian County. 93 severe Dysplasias and 122 mild Dysplasias of the esophagus were selected. By means of an Axiomat-microscope equipped with TV-camera, 100 normal nuclei of well-preserved cells in the intermediate layer of Pap-stained squamous Epithelium were randomly examined. Results: Of the 93 cytologically diagnosed severe Dysplasia cases, 24,14 and 7 progressed to carcinoma in 3, 5 and 9 years, respectively. In the other 48 cases, Dysplasia remained stable or regressed to normal. The other cases were used as the control. According to chromatin features, correct diagnosis of cases was achieved by HRIA in 75.0% (18/24), 85.7% (12/14) and 85.7% (6/7) of the cases examined, respectively (P<0.001). Of the 122 cytologically diagnosed mild Dysplasia, 16,13 and 12 cases progressed to carcinoma in 3, 5 and 9 years, respectively. The other 81 cases remained stable or regressed to normal. Correct diagnosis was made by HRIA in 93.8% (15/16), 76.9% (10/13) and 833% (10/12) of the cases examined, respectively (P<0.001). Conclusion: Chromatin nuclear features examined by HRIA can predict the outcome of precancerous lesions and discriminate progressor from non-progressor ones. It can be used as surrogate endpoint biomarkers for the evaluation of efficiency of chemo-prevention trial.
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Prediction of the outcome of esophageal Epithelium Dysplasia by high resolution image analysis
Chinese Journal of Cancer Research, 2000Co-Authors: Bin Zhou, Zhen-wei Ding, Jixin Wang, Uta Jutting, Karsten Rodenacker, P GaisAbstract:Objective: To predict the outcome of Dysplasia of esophageal Epithelium by means of high resolution image analysis (HRIA). Methods: Asymptomatic adults were examined for balloon cytology of the esophagus in 1983 from Heshun Commune of Linxian County. 93 severe Dysplasias and 122 mild Dysplasias of the esophagus were selected. By means of an Axiomat-microscope equipped with TV-camera, 100 normal nuclei of well-preserved cells in the intermediate layer of Pap-stained squamous Epithelium were randomly examined. Results: Of the 93 cytologically diagnosed severe Dysplasia cases, 24,14 and 7 progressed to carcinoma in 3, 5 and 9 years, respectively. In the other 48 cases, Dysplasia remained stable or regressed to normal. The other cases were used as the control. According to chromatin features, correct diagnosis of cases was achieved by HRIA in 75.0% (18/24), 85.7% (12/14) and 85.7% (6/7) of the cases examined, respectively (P
Masaki Mori - One of the best experts on this subject based on the ideXlab platform.
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Global analysis of altered gene expressions during the process of esophageal squamous cell carcinogenesis in the rat: a study combined with a laser microdissection and a cDNA microarray.
Cancer Research, 2005Co-Authors: Koujiro Nishida, Shinji Mine, Tohru Utsunomiya, Hiroshi Inoue, Masahiro Okamoto, Harushi Udagawa, Taizo Hanai, Masaki MoriAbstract:The genetic alterations that occur during esophageal tumorigenesis have yet to be determined. We previously established a Wister rat carcinogenesis model of esophageal squamous cell carcinoma. To understand more about the molecular mechanisms during carcinogenesis, we produced esophageal neoplastic lesions by administering N-amyl-N-methylnitrosamine and 12-O-tetradecanoylphorbol-13-acetate to rats. We used laser microdissection to specifically isolate the cells from the normal Epithelium, papilloma, Dysplasia, and invasive carcinoma. Using a cDNA microarray representing 14,815 clones, we then analyzed the gene expression profiles for each esophageal lesion. The number of differentially expressed genes compared with the normal control dramatically increased in a step-by-step fashion from normal Epithelium (1,151 ± 119 genes) to papilloma (1,899 ± 543 genes), Dysplasia (1,991 ± 193 genes), and invasive carcinoma (2,756 ± 87 genes). A hierarchical clustering analysis showed that the three stages of normal Epithelium, Dysplasia (papilloma), and invasive carcinoma could be clearly classified, whereas the gene expression patterns of papilloma and Dysplasia were indistinguishable. Using the Fisher criterion, we also identified 50 genes whose expression level had either significantly increased or decreased in a step-by-step manner from the normal Epithelium to Dysplasia and then finally to invasive carcinoma. Many of these genes were not previously known to be associated with esophageal carcinogenesis. The present findings in our rat model thus seem to provide us with a better understanding of the molecular alterations that occur during esophageal carcinogenesis and hopefully will also help lead to the development of novel diagnostic and therapeutic targets.
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expression of sialyl tn antigens in normal squamous Epithelium Dysplasia and squamous cell carcinoma in the esophagus
Cancer Research, 1993Co-Authors: Yoichi Ikeda, Hiroyuki Kuwano, K Baba, Masahiko Ikebe, Tetuya Matushima, Y Adachi, Masaki Mori, Keizo SugimachiAbstract:Abstract Two monoclonal antibodies, TKH2 and B72.3, directed toward the Sialyl-Tn antigen (SA 2, 6GalNAcα-O-Ser/Thr), were examined immunohistochemically to analyze the expression of these antigens in 20 areas of normal squamous Epithelium, 12 lesions of Dysplasia, and 86 cases of squamous cell carcinoma including 32 with superficial carcinoma in the esophagus. No expression of TKH2 or B72.3 was found in the normal squamous Epithelium. Among the 12 lesions of Dysplasia only one expressed TKH2. In carcinoma the expression of TKH2 and B72.3 was found in 40 (47%) and 21 (24%) of the 86 carcinomas, respectively; however, the number of positive malignant cells with TKH2 and B72.3 totaled less than half that in the tissue, and no relationship was found between either prognosis or lymph node metastasis and the expression of Sialyl-Tn antigen. These results indicate that Sialyl-Tn antigen appears in the process of malignant transformation or tumor progression in esophageal squamous cell carcinoma; however, the positive expression of Sialyl-Tn antigen was not directly connected to either prognosis or lymph node metastasis.