The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform

Lorraine E. Williams - One of the best experts on this subject based on the ideXlab platform.

  • Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+ -ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe Cichoracearum.
    Mycological Research, 2006
    Co-Authors: Vasileios Fotopoulos, Robert Holmes, J. L. Hall, Lorraine E. Williams
    Abstract:

    Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H+-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe Cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H+-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen.

  • the monosaccharide transporter gene atstp4 and the cell wall invertase atbetafruct1 are induced in arabidopsis during infection with the fungal biotroph Erysiphe Cichoracearum
    Plant Physiology, 2003
    Co-Authors: Vasileios Fotopoulos, J. L. Hall, Martin J Gilbert, Jon K Pittman, Alison C Marvier, Aram J Buchanan, Norbert Sauer, Lorraine E. Williams
    Abstract:

    Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe Cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.

  • the monosaccharide transporter gene atstp4 and the cell wall invertase atβfruct1 are induced in arabidopsis during infection with the fungal biotroph Erysiphe Cichoracearum
    Plant Physiology, 2003
    Co-Authors: Vasileios Fotopoulos, J. L. Hall, Martin J Gilbert, Jon K Pittman, Alison C Marvier, Aram J Buchanan, Norbert Sauer, Lorraine E. Williams
    Abstract:

    Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe Cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.

Degang Zhao - One of the best experts on this subject based on the ideXlab platform.

  • Overexpression of a New Chitinase Gene EuCHIT2 Enhances Resistance to Erysiphe Cichoracearum DC in Tobacco Plants.
    International journal of molecular sciences, 2017
    Co-Authors: Dong Xuan, Yichen Zhao, Ran Xin, Guo Linxia, Degang Zhao
    Abstract:

    In this study, we cloned a new chitinase gene, EuCHIT2, from Eucommia ulmoides Oliver (E. ulmoides) using rapid amplification of cDNA ends (RACE) technology and constructed an overexpression vector, pSH-35S-EuCHIT2, to introduce it into tobacco (Nicotiana tabacum cv. Xanthi). Resistance to Erysiphe Cichoracearum de Candolle (E.Cichoracearum DC) and molecular mechanisms in the transgenic tobacco were determined by drop inoculation, spore counting, determination of physicochemical indicators, and analysis of gene expression. The chitinase activity and resistance to E. Cichoracearum DC were significantly higher in the transgenic tobacco than in wild-type tobacco (p < 0.05). The activities of peroxidase (POD) and catalase (CAT), after inoculation with E. Cichoracearum DC, were higher in the transgenic tobacco than in the wild-type. Conversely, the malondialdehyde (MDA) content was significantly lower in the transgenic tobacco than the wild-type before and after inoculation. In addition, our study also indicated that the resistance to E. Cichoracearum DC might involve the salicylic acid (SA) and jasmonic acid (JA) pathways, because the expression levels of pathogenesis-related gene 1 (PR-1a) and coronatine-insensitive 1 (COI1) were significantly increased and decreased, respectively, after inoculation with E. Cichoracearum DC. The present study supports the notion that PR-1a and POD participate in resistance to E. Cichoracearum DC in the transgenic tobacco plants.

Vasileios Fotopoulos - One of the best experts on this subject based on the ideXlab platform.

  • Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+ -ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe Cichoracearum.
    Mycological Research, 2006
    Co-Authors: Vasileios Fotopoulos, Robert Holmes, J. L. Hall, Lorraine E. Williams
    Abstract:

    Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H+-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe Cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H+-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen.

  • the monosaccharide transporter gene atstp4 and the cell wall invertase atbetafruct1 are induced in arabidopsis during infection with the fungal biotroph Erysiphe Cichoracearum
    Plant Physiology, 2003
    Co-Authors: Vasileios Fotopoulos, J. L. Hall, Martin J Gilbert, Jon K Pittman, Alison C Marvier, Aram J Buchanan, Norbert Sauer, Lorraine E. Williams
    Abstract:

    Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe Cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.

  • the monosaccharide transporter gene atstp4 and the cell wall invertase atβfruct1 are induced in arabidopsis during infection with the fungal biotroph Erysiphe Cichoracearum
    Plant Physiology, 2003
    Co-Authors: Vasileios Fotopoulos, J. L. Hall, Martin J Gilbert, Jon K Pittman, Alison C Marvier, Aram J Buchanan, Norbert Sauer, Lorraine E. Williams
    Abstract:

    Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe Cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.

Dong Xuan - One of the best experts on this subject based on the ideXlab platform.

  • Overexpression of a New Chitinase Gene EuCHIT2 Enhances Resistance to Erysiphe Cichoracearum DC in Tobacco Plants.
    International journal of molecular sciences, 2017
    Co-Authors: Dong Xuan, Yichen Zhao, Ran Xin, Guo Linxia, Degang Zhao
    Abstract:

    In this study, we cloned a new chitinase gene, EuCHIT2, from Eucommia ulmoides Oliver (E. ulmoides) using rapid amplification of cDNA ends (RACE) technology and constructed an overexpression vector, pSH-35S-EuCHIT2, to introduce it into tobacco (Nicotiana tabacum cv. Xanthi). Resistance to Erysiphe Cichoracearum de Candolle (E.Cichoracearum DC) and molecular mechanisms in the transgenic tobacco were determined by drop inoculation, spore counting, determination of physicochemical indicators, and analysis of gene expression. The chitinase activity and resistance to E. Cichoracearum DC were significantly higher in the transgenic tobacco than in wild-type tobacco (p < 0.05). The activities of peroxidase (POD) and catalase (CAT), after inoculation with E. Cichoracearum DC, were higher in the transgenic tobacco than in the wild-type. Conversely, the malondialdehyde (MDA) content was significantly lower in the transgenic tobacco than the wild-type before and after inoculation. In addition, our study also indicated that the resistance to E. Cichoracearum DC might involve the salicylic acid (SA) and jasmonic acid (JA) pathways, because the expression levels of pathogenesis-related gene 1 (PR-1a) and coronatine-insensitive 1 (COI1) were significantly increased and decreased, respectively, after inoculation with E. Cichoracearum DC. The present study supports the notion that PR-1a and POD participate in resistance to E. Cichoracearum DC in the transgenic tobacco plants.

J. L. Hall - One of the best experts on this subject based on the ideXlab platform.

  • Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+ -ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe Cichoracearum.
    Mycological Research, 2006
    Co-Authors: Vasileios Fotopoulos, Robert Holmes, J. L. Hall, Lorraine E. Williams
    Abstract:

    Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H+-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe Cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H+-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen.

  • the monosaccharide transporter gene atstp4 and the cell wall invertase atbetafruct1 are induced in arabidopsis during infection with the fungal biotroph Erysiphe Cichoracearum
    Plant Physiology, 2003
    Co-Authors: Vasileios Fotopoulos, J. L. Hall, Martin J Gilbert, Jon K Pittman, Alison C Marvier, Aram J Buchanan, Norbert Sauer, Lorraine E. Williams
    Abstract:

    Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe Cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.

  • the monosaccharide transporter gene atstp4 and the cell wall invertase atβfruct1 are induced in arabidopsis during infection with the fungal biotroph Erysiphe Cichoracearum
    Plant Physiology, 2003
    Co-Authors: Vasileios Fotopoulos, J. L. Hall, Martin J Gilbert, Jon K Pittman, Alison C Marvier, Aram J Buchanan, Norbert Sauer, Lorraine E. Williams
    Abstract:

    Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe Cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.