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Alain Sommier - One of the best experts on this subject based on the ideXlab platform.
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characterization of different sugar alcohols as phase change materials for thermal energy storage applications
Solar Energy Materials and Solar Cells, 2017Co-Authors: Palomo E Del Barrio, M Duquesne, Julien Daranlot, J Jolly, W G Alshaer, T Kouadio, Antoine Godin, Alain SommierAbstract:Abstract Sugar alcohols (SA) are attractive phase change materials (PCM) for thermal energy storage applications at low-to-medium temperatures (70–180 °C). Five pure sugar alcohols (xylitol, adonitol, L-arabitol, Erythritol, D-mannitol) and three eutectic blends (eythritol/xylitol, L-arabitol/Erythritol, L-arabitol/xylitol) are investigated in this paper. Experimental characterization of such materials as PCMs is provided. This encompasses the measurement of their melting point and latent heat of fusion, as well as the experimental determination of all key physical properties (specific heat, thermal conductivity, thermal diffusivity, density, viscosity) as a function of the temperature. The performances of the studied materials are compared to those of most currently used PCMs (paraffin waxes, salt hydrates etc.) in the field of thermal energy storage. The most significant applications, including solar seasonal energy storage, are also discussed.
Aleksandra M. Mirończuk - One of the best experts on this subject based on the ideXlab platform.
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A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism.
Frontiers in Microbiology, 2018Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Karolina Zugaj, Dorota A. Rzechonek, Adam DobrowolskiAbstract:Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of Erythritol utilization (EUF1), the metabolic pathway of Erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in Erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on Erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired Erythritol assimilation, whereas synthesis of Erythritol remained unchanged. The results showed that YALI0F1628g is involved in Erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of Erythritol assimilation in yeast Y. lipolytica.
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Data_Sheet_1_A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism.docx
2018Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Karolina Zugaj, Dorota A. Rzechonek, Adam DobrowolskiAbstract:Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of Erythritol utilization (EUF1), the metabolic pathway of Erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in Erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on Erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired Erythritol assimilation, whereas synthesis of Erythritol remained unchanged. The results showed that YALI0F1628g is involved in Erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of Erythritol assimilation in yeast Y. lipolytica.
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Recent advances in biological production of Erythritol
Critical Reviews in Biotechnology, 2017Co-Authors: Dorota A. Rzechonek, Adam Dobrowolski, Waldemar Rymowicz, Aleksandra M. MirończukAbstract:Erythritol is a natural sweetener commonly used in the food and pharmaceutical industries. Produced by microorganisms as an osmoprotectant, it is an ideal sucrose substitute for diabetics or overweight persons due to its almost zero calorie content. Currently, Erythritol is produced on an industrial scale through the fermentation of sugars by some yeasts, such as Moniliella sp. However, the popularity of Erythritol as a sweetener is still small because of its high retail price. This creates an opportunity for further process improvement. Recent years have brought the rapid development of Erythritol biosynthesis methods from the low-cost substrates, and a better understanding of the metabolic pathways leading to Erythritol synthesis. The yeast Yarrowia lipolytica emerges as an organism effectively producing Erythritol from pure or crude glycerol. Moreover, novel Erythritol producing organisms and substrates may be taken into considerations due to metabolic engineering. This review focuses on the modification of Erythritol production to use low-cost substrates and metabolic engineering of the microorganisms in order to improve yield and productivity.
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functional overexpression of genes involved in Erythritol synthesis in the yeast yarrowia lipolytica
Biotechnology for Biofuels, 2017Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Adam DobrowolskiAbstract:Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an Erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that Erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as Erythritol. Glycerol is a renewable feedstock which is produced on a large scale as a waste product by many branches of industry. In this study, we functionally overexpressed four genes involved in the pentose phosphate pathway (PPP): gene YALI0E06479g encoding transketolase (TKL1), gene YALI0F15587g encoding transaldolase (TAL1), gene YALI0E22649g encoding glucose-6-phosphate dehydrogenase (ZWF1), and gene YALI0B15598g encoding 6-phosphogluconate dehydrogenase (GND1). Here, we show that the crucial gene for Erythritol synthesis in Y. lipolytica is transketolase. Overexpression of this gene results in a twofold improvement in Erythritol synthesis during a shake-flask experiment (58 g/L). Moreover, overexpression of TKL1 allows for efficient production of Erythritol independently from the supplied dissolved oxygen. Fermentation conducted in a 5-L bioreactor at low agitation results in almost 70% higher titer of Erythritol over the control strain. This work presents the importance of the PPP in Erythritol synthesis and the feasibility for economic production of Erythritol from glycerol by the yeast Y. lipolytica.
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EUF1 - a newly identified gene involved in Erythritol utilization in Yarrowia lipolytica
Scientific Reports, 2017Co-Authors: Dorota A. Rzechonek, Waldemar Rymowicz, Cécile Neuvéglise, Hugo Devillers, Aleksandra M. MirończukAbstract:The gene YALI0F01562g was identified as an important factor involved in Erythritol catabolism of the unconventional yeast Yarrowia lipolytica. Its putative role was identified for the first time by comparative analysis of four Y. lipolytica strains: A-101.1.31, Wratislavia K1, MK1 and AMM. The presence of a mutation that seriously damaged the gene corresponded to inability of the strain Wratislavia K1 to utilize Erythritol. RT-PCR analysis of the strain MK1 demonstrated a significant increase in YALI0F01562g expression during growth on Erythritol. Further studies involving deletion and overexpression of the selected gene showed that it is indeed essential for efficient Erythritol assimilation. The deletion strain Y. lipolytica AMM Delta euf1 was almost unable to grow on Erythritol as the sole carbon source. When the strain was applied in the process of Erythritol production from glycerol, the amount of Erythritol remained constant after reaching the maximal concentration. Analysis of the YALI0F01562g gene sequence revealed the presence of domains characteristic for transcription factors. Therefore we suggest naming the studied gene Erythritol Utilization Factor - EUF1.
Palomo E Del Barrio - One of the best experts on this subject based on the ideXlab platform.
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characterization of different sugar alcohols as phase change materials for thermal energy storage applications
Solar Energy Materials and Solar Cells, 2017Co-Authors: Palomo E Del Barrio, M Duquesne, Julien Daranlot, J Jolly, W G Alshaer, T Kouadio, Antoine Godin, Alain SommierAbstract:Abstract Sugar alcohols (SA) are attractive phase change materials (PCM) for thermal energy storage applications at low-to-medium temperatures (70–180 °C). Five pure sugar alcohols (xylitol, adonitol, L-arabitol, Erythritol, D-mannitol) and three eutectic blends (eythritol/xylitol, L-arabitol/Erythritol, L-arabitol/xylitol) are investigated in this paper. Experimental characterization of such materials as PCMs is provided. This encompasses the measurement of their melting point and latent heat of fusion, as well as the experimental determination of all key physical properties (specific heat, thermal conductivity, thermal diffusivity, density, viscosity) as a function of the temperature. The performances of the studied materials are compared to those of most currently used PCMs (paraffin waxes, salt hydrates etc.) in the field of thermal energy storage. The most significant applications, including solar seasonal energy storage, are also discussed.
Adam Dobrowolski - One of the best experts on this subject based on the ideXlab platform.
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A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism.
Frontiers in Microbiology, 2018Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Karolina Zugaj, Dorota A. Rzechonek, Adam DobrowolskiAbstract:Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of Erythritol utilization (EUF1), the metabolic pathway of Erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in Erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on Erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired Erythritol assimilation, whereas synthesis of Erythritol remained unchanged. The results showed that YALI0F1628g is involved in Erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of Erythritol assimilation in yeast Y. lipolytica.
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Data_Sheet_1_A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism.docx
2018Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Karolina Zugaj, Dorota A. Rzechonek, Adam DobrowolskiAbstract:Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of Erythritol utilization (EUF1), the metabolic pathway of Erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in Erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on Erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired Erythritol assimilation, whereas synthesis of Erythritol remained unchanged. The results showed that YALI0F1628g is involved in Erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of Erythritol assimilation in yeast Y. lipolytica.
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Recent advances in biological production of Erythritol
Critical Reviews in Biotechnology, 2017Co-Authors: Dorota A. Rzechonek, Adam Dobrowolski, Waldemar Rymowicz, Aleksandra M. MirończukAbstract:Erythritol is a natural sweetener commonly used in the food and pharmaceutical industries. Produced by microorganisms as an osmoprotectant, it is an ideal sucrose substitute for diabetics or overweight persons due to its almost zero calorie content. Currently, Erythritol is produced on an industrial scale through the fermentation of sugars by some yeasts, such as Moniliella sp. However, the popularity of Erythritol as a sweetener is still small because of its high retail price. This creates an opportunity for further process improvement. Recent years have brought the rapid development of Erythritol biosynthesis methods from the low-cost substrates, and a better understanding of the metabolic pathways leading to Erythritol synthesis. The yeast Yarrowia lipolytica emerges as an organism effectively producing Erythritol from pure or crude glycerol. Moreover, novel Erythritol producing organisms and substrates may be taken into considerations due to metabolic engineering. This review focuses on the modification of Erythritol production to use low-cost substrates and metabolic engineering of the microorganisms in order to improve yield and productivity.
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functional overexpression of genes involved in Erythritol synthesis in the yeast yarrowia lipolytica
Biotechnology for Biofuels, 2017Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Adam DobrowolskiAbstract:Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an Erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that Erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as Erythritol. Glycerol is a renewable feedstock which is produced on a large scale as a waste product by many branches of industry. In this study, we functionally overexpressed four genes involved in the pentose phosphate pathway (PPP): gene YALI0E06479g encoding transketolase (TKL1), gene YALI0F15587g encoding transaldolase (TAL1), gene YALI0E22649g encoding glucose-6-phosphate dehydrogenase (ZWF1), and gene YALI0B15598g encoding 6-phosphogluconate dehydrogenase (GND1). Here, we show that the crucial gene for Erythritol synthesis in Y. lipolytica is transketolase. Overexpression of this gene results in a twofold improvement in Erythritol synthesis during a shake-flask experiment (58 g/L). Moreover, overexpression of TKL1 allows for efficient production of Erythritol independently from the supplied dissolved oxygen. Fermentation conducted in a 5-L bioreactor at low agitation results in almost 70% higher titer of Erythritol over the control strain. This work presents the importance of the PPP in Erythritol synthesis and the feasibility for economic production of Erythritol from glycerol by the yeast Y. lipolytica.
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Functional overexpression of genes involved in Erythritol synthesis in the yeast Yarrowia lipolytica
Biotechnology for Biofuels, 2017Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Adam DobrowolskiAbstract:Background Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an Erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that Erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as Erythritol. Glycerol is a renewable feedstock which is produced on a large scale as a waste product by many branches of industry. Results In this study, we functionally overexpressed four genes involved in the pentose phosphate pathway (PPP): gene YALI0E06479 g encoding transketolase ( TKL1 ), gene YALI0F15587 g encoding transaldolase ( TAL1 ), gene YALI0E22649 g encoding glucose-6-phosphate dehydrogenase ( ZWF1 ), and gene YALI0B15598 g encoding 6-phosphogluconate dehydrogenase ( GND1 ). Here, we show that the crucial gene for Erythritol synthesis in Y. lipolytica is transketolase. Overexpression of this gene results in a twofold improvement in Erythritol synthesis during a shake-flask experiment (58 g/L). Moreover, overexpression of TKL1 allows for efficient production of Erythritol independently from the supplied dissolved oxygen. Fermentation conducted in a 5-L bioreactor at low agitation results in almost 70% higher titer of Erythritol over the control strain. Conclusion This work presents the importance of the PPP in Erythritol synthesis and the feasibility for economic production of Erythritol from glycerol by the yeast Y. lipolytica .
Waldemar Rymowicz - One of the best experts on this subject based on the ideXlab platform.
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Recent advances in biological production of Erythritol
Critical Reviews in Biotechnology, 2017Co-Authors: Dorota A. Rzechonek, Adam Dobrowolski, Waldemar Rymowicz, Aleksandra M. MirończukAbstract:Erythritol is a natural sweetener commonly used in the food and pharmaceutical industries. Produced by microorganisms as an osmoprotectant, it is an ideal sucrose substitute for diabetics or overweight persons due to its almost zero calorie content. Currently, Erythritol is produced on an industrial scale through the fermentation of sugars by some yeasts, such as Moniliella sp. However, the popularity of Erythritol as a sweetener is still small because of its high retail price. This creates an opportunity for further process improvement. Recent years have brought the rapid development of Erythritol biosynthesis methods from the low-cost substrates, and a better understanding of the metabolic pathways leading to Erythritol synthesis. The yeast Yarrowia lipolytica emerges as an organism effectively producing Erythritol from pure or crude glycerol. Moreover, novel Erythritol producing organisms and substrates may be taken into considerations due to metabolic engineering. This review focuses on the modification of Erythritol production to use low-cost substrates and metabolic engineering of the microorganisms in order to improve yield and productivity.
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EUF1 - a newly identified gene involved in Erythritol utilization in Yarrowia lipolytica
Scientific Reports, 2017Co-Authors: Dorota A. Rzechonek, Waldemar Rymowicz, Cécile Neuvéglise, Hugo Devillers, Aleksandra M. MirończukAbstract:The gene YALI0F01562g was identified as an important factor involved in Erythritol catabolism of the unconventional yeast Yarrowia lipolytica. Its putative role was identified for the first time by comparative analysis of four Y. lipolytica strains: A-101.1.31, Wratislavia K1, MK1 and AMM. The presence of a mutation that seriously damaged the gene corresponded to inability of the strain Wratislavia K1 to utilize Erythritol. RT-PCR analysis of the strain MK1 demonstrated a significant increase in YALI0F01562g expression during growth on Erythritol. Further studies involving deletion and overexpression of the selected gene showed that it is indeed essential for efficient Erythritol assimilation. The deletion strain Y. lipolytica AMM Delta euf1 was almost unable to grow on Erythritol as the sole carbon source. When the strain was applied in the process of Erythritol production from glycerol, the amount of Erythritol remained constant after reaching the maximal concentration. Analysis of the YALI0F01562g gene sequence revealed the presence of domains characteristic for transcription factors. Therefore we suggest naming the studied gene Erythritol Utilization Factor - EUF1.
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Technology of efficient continuous Erythritol production from glycerol
Journal of Cleaner Production, 2016Co-Authors: Magdalena Rakicka, Beata Rukowicz, Anita Rywińska, Zbigniew Lazar, Waldemar RymowiczAbstract:Abstract Erythritol production in chemostat culture from pure and crude glycerol with different nitrogen sources by Yarrowia lipolytica Wratislavia K1 was investigated. Moreover, a process of Erythritol purification based on ion exchange is proposed. The highest Erythritol production (103.4 g L −1 with a volumetric Erythritol production rate of 1.12 g L −1 h −1 and a yield of 0.52 g g −1 ) was obtained by applying an inorganic nitrogen source (4.6 g L −1 of ammonium sulfate). Very promising results were also obtained when pure glycerol was replaced with crude glycerol as a carbon source. During this culture yeast produced 81.9 g L −1 of Erythritol, which corresponded to a 0.9 g L −1 h −1 volumetric Erythritol production rate and a yield of Erythritol production of 0.4 g g −1 . The fermentation broth was desalinated and decolorized by ion exclusion, ion exchange and sorption on activated carbon. The final solution contained only Erythritol. The presented technology follows the vision of a circular economy by turning waste into products and using renewable materials as a feedstock. This study supports the potential of the industrially relevant wild type Yarrowia lipolytica strain for intensified and efficient Erythritol production. The proposed technology is ecofriendly, requires a small number of purification steps and generates low amount of wastes. The final solution is rich in Erythritol and can be applied directly as a sweet low-calorie food additive.
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Efficient utilization of inulin and glycerol as fermentation substrates in Erythritol and citric acid production using Yarrowia lipolytica expressing inulinase
Chemical Papers, 2016Co-Authors: Magdalena Rakicka, Zbigniew Lazar, Anita Rywińska, Waldemar RymowiczAbstract:Inulin and glycerol were used as substrates for efficient Erythritol and citric acid production by newly engineered Yarrowia lipolytica strains. Hydrolysis of inulin by the Y. lipolytica Wratislavia K1 strain was established by expressing the Kluyveromyces marxianus INU1 gene. Erythritol was produced in two stages: inulin was used for biomass formation, followed by Erythritol biosynthesis initiated by glycerol addition. The highest titer of Erythritol obtained, 120.9 g L^−1 with the yield of 0.6 g g^−1, was produced by the K1 INU 6 strain. Moreover, the K1 INU 6 strain in fed-batch culture produced a high amount of citric acid: 105.2 g L^−1 after 235 h from 200 g L^−1 of inulin. Maximum activity of inulinase during this culture was 14000 U g^−1 of cell dry mass. The presented study proves the potential of new Y. lipolytica transformants for efficient Erythritol and citric acid production from inexpensive raw materials such as inulin and glycerol.
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a two stage fermentation process of Erythritol production by yeast y lipolytica from molasses and glycerol
Bioresource Technology, 2015Co-Authors: Aleksandra M. Mirończuk, Anna Biegalska, Waldemar Rymowicz, Magdalena Rakicka, Adam DobrowolskiAbstract:In this study, a two-stage fermentation process of Erythritol production based on molasses and glycerol was investigated. During the first stage, the biomass of Yarrowia lipolytica was grown on medium containing sucrose as the sole carbon source. In the second stage, production of Erythritol was initiated by glycerol addition. To use molasses as a substrate for Erythritol synthesis, sucrose utilization was established by expressing the Saccharomyces cerevisiae SUC2 gene. In this study, cultivation of yeast Y. lipolytica could produce 52-114 g/L of Erythritol. The productivity was 0.58-1.04 g/L/h, and yield was 0.26-0.57 g/g; the final biomasses yield ranged 17-41 g/L. This is the first report describing Erythritol production via industrial raw molasses and glycerol by Y. lipolytica. This work uses genetically modified strains of Y. lipolytica as tool for the direct conversion of affordable raw industrial molasses and glycerol into the value-added Erythritol product.