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Michael S Feld - One of the best experts on this subject based on the ideXlab platform.

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Kamra Adizadega, Ramachandra R Dasari, Michael S Feld
    Abstract:

    We present a new quantitative method for in- vestigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suit- able for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic mor- phology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis. © 2005 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2149847

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Ramachandra R Dasari, Catherine A Best, Kamran Badizadegan, Michael S Feld
    Abstract:

    We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.

Etheresia Pretorius - One of the best experts on this subject based on the ideXlab platform.

  • the effect of endogenous and synthetic estrogens on whole blood clot formation and Erythrocyte Structure
    Microscopy and Microanalysis, 2017
    Co-Authors: Albe C Swanepoel, Odette Emmerson, Etheresia Pretorius
    Abstract:

    As Erythrocyte and estrogens interact so closely and Erythrocytes can indicate the healthiness of an individual, it is essential to investigate the effects of natural estrogens as well as synthetic estrogens on these cells. Whole blood samples were used for thromboelastography (TEG), light microscopy (LM), and scanning electron microscopy (SEM) investigation. Viscoelastic investigation with TEG revealed that estrogens affected the rate of clot formation without any significant effect on the strength or stability of the clot. Axial ratio analysis with LM showed a statistically significant increase in number of Erythrocytes with decreased roundness. Morphological analysis with SEM confirmed the change in Erythrocyte shape and revealed both ultrastructural membrane changes and Erythrocyte interactions. As Erythrocyte shape and membrane flexibility correlates to physiological functioning of these cells in circulation, these changes, indicative of possible eryptosis brought on by estrogens, when experienced by individuals with an underlying inflammatory or hematological illness, could impair Erythrocyte functioning and even result in obstructions in circulation. In conclusion, we suggest that whole blood analysis with viscoelastic and morphological techniques could be used as assessment of the hematological healthiness of individuals using estrogens.

  • effect of progesterone and synthetic progestins on whole blood clot formation and Erythrocyte Structure
    Microscopy and Microanalysis, 2017
    Co-Authors: Albe C Swanepoel, Odette Emmerson, Etheresia Pretorius
    Abstract:

    Combined oral contraceptive (COC) use is a risk factor for venous thrombosis (VT) and related to the specific type of progestin used. VT is accompanied by inflammation and pathophysiological clot formation, that includes aberrant Erythrocytes and fibrin(ogen) interactions. In this paper, we aim to determine the influence of progesterone and different synthetic progestins found in COCs on the viscoelasticity of whole blood clots, as well as Erythrocyte morphology and membrane ultraStructure, in an in vitro laboratory study. Thromboelastography (TEG), light microscopy, and scanning electron microscopy were our chosen methods. Our results point out that progestins influence the rate of whole blood clot formation. Alterations to Erythrocyte morphology and membrane ultraStructure suggest the presence of eryptosis. We also note increased rouleaux formation, Erythrocyte aggregation, and spontaneous fibrin formation in whole blood which may explain the increased risk of VT associated with COC use. Although not all COC users will experience a thrombotic event, individuals with a thrombotic predisposition, due to inflammatory or hematological illness, should be closely monitored to prevent pathological thrombosis.

  • effects of il 1β il 6 and il 8 on Erythrocytes platelets and clot viscoelasticity
    Scientific Reports, 2016
    Co-Authors: Janette Bester, Etheresia Pretorius
    Abstract:

    Complex interactions exist between cytokines, and the interleukin family plays a fundamental role in inflammation. Particularly circulating IL-1β, IL-6 and IL-8 are unregulated in systemic and chronic inflammatory conditions. Hypercoagulability is an important hallmark of inflammation, and these cytokines are critically involved in abnormal clot formation, Erythrocyte pathology and platelet hyper-activation, and these three cytokines have known receptors on platelets. Although these cytokines are always unregulated in inflammation, we do not know how the individual cytokines act upon the Structure of Erythrocytes and platelets, and which of the viscoelastic clot parameters are changed. Here we study the effects of IL-1β, IL-6 and IL-8 at low physiological levels, representative of chronic inflammation, by using scanning electron microscopy and thromboelastography. All three interleukins caused the viscoelastic properties to display an increased hypercoagulability of whole blood and pathology of both Erythrocytes and platelets. The most pronounced changes were noted where all three cytokines caused platelet hyper-activation and spreading. Erythrocyte Structure was notably affected in the presence of IL-8, where the morphological changes resembled that typically seen in eryptosis (programmed cell death). We suggest that Erythrocytes and platelets are particularly sensitive to cytokine presence, and that they are excellent health indicators.

Gabriel Popescu - One of the best experts on this subject based on the ideXlab platform.

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Kamra Adizadega, Ramachandra R Dasari, Michael S Feld
    Abstract:

    We present a new quantitative method for in- vestigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suit- able for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic mor- phology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis. © 2005 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2149847

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Ramachandra R Dasari, Catherine A Best, Kamran Badizadegan, Michael S Feld
    Abstract:

    We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.

Ramachandra R Dasari - One of the best experts on this subject based on the ideXlab platform.

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Kamra Adizadega, Ramachandra R Dasari, Michael S Feld
    Abstract:

    We present a new quantitative method for in- vestigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suit- able for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic mor- phology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis. © 2005 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2149847

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Ramachandra R Dasari, Catherine A Best, Kamran Badizadegan, Michael S Feld
    Abstract:

    We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.

Takahiro Ikeda - One of the best experts on this subject based on the ideXlab platform.

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Kamra Adizadega, Ramachandra R Dasari, Michael S Feld
    Abstract:

    We present a new quantitative method for in- vestigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suit- able for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic mor- phology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis. © 2005 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2149847

  • Erythrocyte Structure and dynamics quantified by hilbert phase microscopy
    Journal of Biomedical Optics, 2005
    Co-Authors: Gabriel Popescu, Takahiro Ikeda, Ramachandra R Dasari, Catherine A Best, Kamran Badizadegan, Michael S Feld
    Abstract:

    We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live Erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.