The Experts below are selected from a list of 1878 Experts worldwide ranked by ideXlab platform

David E. Housman - One of the best experts on this subject based on the ideXlab platform.

  • characterization of an Erythroid Precursor Cell of high proliferative capacity in normal human peripheral blood erythropoiesis blood stem Cells lymphocytes
    2016
    Co-Authors: Bryan J. Clarke, David E. Housman
    Abstract:

    We have found that the peripheral blood of normal humans contains a significant number of committed Erythroid stem Cells of high proliferative capacity. These Erythroid stem Cells closely resemble the murine Erythroid burst-forming unit (BFU-E) with respect to proliferative ca- pacity, colony morphology, and erythropoietin requirement. BFU-E were isolated from the peripheral blood of normal in- dividuals by Ficoll-Hypaque density gradient centrifugation and cultured in vitro using the plasma culture technique. Macroscopic Erythroid colonies of between 100 and 1000 Cells were observed after 10-14 days of culture in the presence of either sheep or human erythropoietin at 0.5-4 units/ml. Indi- vidual colonies contained between 3 and 20 subcolonies and reached a maximum mean size of approximately 500 Cells. Colony number was linearly related to the Cell input, suggesting that a single Cellular entity was Precursor to each colony. The frequency of Cells capable of giving rise to an Erythroid colony was at least 100 per ml of blood in a number of individuals tested. The ability to assay significant numbers of Erythroid Precursor Cells of high proliferative capacity from normal pe- ripheral blood should facilitate the study of both normal erythropoiesis and of disease states affecting erythropoiesis in which marrow samples are not available on a routine basis. The analysis of erythropoiesis in the mouse has been advanced significantly by the development and application of techniques for cloning committed Erythroid Precursor Cells in vitro (1-5). Recently, these techniques have been extended for use with human Erythroid Precursor Cells (6, 7). In the mouse, Heath et al. clearly demonstrate the existence of two distinct Cell pop- ulations in the marrow, BFU-E (burst-forming unit that re- sponds to erythropoietin) and CFU-E (colony-forming unit that responds to erythropoietin) (8). These populations can be dis- tinguished by a number of characteristics, including Cell vol- ume, responsiveness to erythropoietin, and proliferative ca- pacity. Axelrad et al. (4, 8) have proposed that BFU-E, a Cell

  • Characterization of an Erythroid Precursor Cell of high proliferative capacity in normal human peripheral blood (erythropoiesis/blood stem Cells/lymphocytes)
    2016
    Co-Authors: Bryan J. Clarke, David E. Housman
    Abstract:

    We have found that the peripheral blood of normal humans contains a significant number of committed Erythroid stem Cells of high proliferative capacity. These Erythroid stem Cells closely resemble the murine Erythroid burst-forming unit (BFU-E) with respect to proliferative ca- pacity, colony morphology, and erythropoietin requirement. BFU-E were isolated from the peripheral blood of normal in- dividuals by Ficoll-Hypaque density gradient centrifugation and cultured in vitro using the plasma culture technique. Macroscopic Erythroid colonies of between 100 and 1000 Cells were observed after 10-14 days of culture in the presence of either sheep or human erythropoietin at 0.5-4 units/ml. Indi- vidual colonies contained between 3 and 20 subcolonies and reached a maximum mean size of approximately 500 Cells. Colony number was linearly related to the Cell input, suggesting that a single Cellular entity was Precursor to each colony. The frequency of Cells capable of giving rise to an Erythroid colony was at least 100 per ml of blood in a number of individuals tested. The ability to assay significant numbers of Erythroid Precursor Cells of high proliferative capacity from normal pe- ripheral blood should facilitate the study of both normal erythropoiesis and of disease states affecting erythropoiesis in which marrow samples are not available on a routine basis. The analysis of erythropoiesis in the mouse has been advanced significantly by the development and application of techniques for cloning committed Erythroid Precursor Cells in vitro (1-5). Recently, these techniques have been extended for use with human Erythroid Precursor Cells (6, 7). In the mouse, Heath et al. clearly demonstrate the existence of two distinct Cell pop- ulations in the marrow, BFU-E (burst-forming unit that re- sponds to erythropoietin) and CFU-E (colony-forming unit that responds to erythropoietin) (8). These populations can be dis- tinguished by a number of characteristics, including Cell vol- ume, responsiveness to erythropoietin, and proliferative ca- pacity. Axelrad et al. (4, 8) have proposed that BFU-E, a Cell

Bryan J. Clarke - One of the best experts on this subject based on the ideXlab platform.

  • characterization of an Erythroid Precursor Cell of high proliferative capacity in normal human peripheral blood erythropoiesis blood stem Cells lymphocytes
    2016
    Co-Authors: Bryan J. Clarke, David E. Housman
    Abstract:

    We have found that the peripheral blood of normal humans contains a significant number of committed Erythroid stem Cells of high proliferative capacity. These Erythroid stem Cells closely resemble the murine Erythroid burst-forming unit (BFU-E) with respect to proliferative ca- pacity, colony morphology, and erythropoietin requirement. BFU-E were isolated from the peripheral blood of normal in- dividuals by Ficoll-Hypaque density gradient centrifugation and cultured in vitro using the plasma culture technique. Macroscopic Erythroid colonies of between 100 and 1000 Cells were observed after 10-14 days of culture in the presence of either sheep or human erythropoietin at 0.5-4 units/ml. Indi- vidual colonies contained between 3 and 20 subcolonies and reached a maximum mean size of approximately 500 Cells. Colony number was linearly related to the Cell input, suggesting that a single Cellular entity was Precursor to each colony. The frequency of Cells capable of giving rise to an Erythroid colony was at least 100 per ml of blood in a number of individuals tested. The ability to assay significant numbers of Erythroid Precursor Cells of high proliferative capacity from normal pe- ripheral blood should facilitate the study of both normal erythropoiesis and of disease states affecting erythropoiesis in which marrow samples are not available on a routine basis. The analysis of erythropoiesis in the mouse has been advanced significantly by the development and application of techniques for cloning committed Erythroid Precursor Cells in vitro (1-5). Recently, these techniques have been extended for use with human Erythroid Precursor Cells (6, 7). In the mouse, Heath et al. clearly demonstrate the existence of two distinct Cell pop- ulations in the marrow, BFU-E (burst-forming unit that re- sponds to erythropoietin) and CFU-E (colony-forming unit that responds to erythropoietin) (8). These populations can be dis- tinguished by a number of characteristics, including Cell vol- ume, responsiveness to erythropoietin, and proliferative ca- pacity. Axelrad et al. (4, 8) have proposed that BFU-E, a Cell

  • Characterization of an Erythroid Precursor Cell of high proliferative capacity in normal human peripheral blood (erythropoiesis/blood stem Cells/lymphocytes)
    2016
    Co-Authors: Bryan J. Clarke, David E. Housman
    Abstract:

    We have found that the peripheral blood of normal humans contains a significant number of committed Erythroid stem Cells of high proliferative capacity. These Erythroid stem Cells closely resemble the murine Erythroid burst-forming unit (BFU-E) with respect to proliferative ca- pacity, colony morphology, and erythropoietin requirement. BFU-E were isolated from the peripheral blood of normal in- dividuals by Ficoll-Hypaque density gradient centrifugation and cultured in vitro using the plasma culture technique. Macroscopic Erythroid colonies of between 100 and 1000 Cells were observed after 10-14 days of culture in the presence of either sheep or human erythropoietin at 0.5-4 units/ml. Indi- vidual colonies contained between 3 and 20 subcolonies and reached a maximum mean size of approximately 500 Cells. Colony number was linearly related to the Cell input, suggesting that a single Cellular entity was Precursor to each colony. The frequency of Cells capable of giving rise to an Erythroid colony was at least 100 per ml of blood in a number of individuals tested. The ability to assay significant numbers of Erythroid Precursor Cells of high proliferative capacity from normal pe- ripheral blood should facilitate the study of both normal erythropoiesis and of disease states affecting erythropoiesis in which marrow samples are not available on a routine basis. The analysis of erythropoiesis in the mouse has been advanced significantly by the development and application of techniques for cloning committed Erythroid Precursor Cells in vitro (1-5). Recently, these techniques have been extended for use with human Erythroid Precursor Cells (6, 7). In the mouse, Heath et al. clearly demonstrate the existence of two distinct Cell pop- ulations in the marrow, BFU-E (burst-forming unit that re- sponds to erythropoietin) and CFU-E (colony-forming unit that responds to erythropoietin) (8). These populations can be dis- tinguished by a number of characteristics, including Cell vol- ume, responsiveness to erythropoietin, and proliferative ca- pacity. Axelrad et al. (4, 8) have proposed that BFU-E, a Cell

Gregory A Denomme - One of the best experts on this subject based on the ideXlab platform.

  • hemolytic disease of the fetus and newborn due to anti ge3 combined antibody dependent hemolysis and Erythroid Precursor Cell growth inhibition
    American Journal of Perinatology, 2008
    Co-Authors: Douglas P Blackall, Gina D Pesek, Matthew M Montgomery, Krishna K Oza, Patricia A Arndt, George Garratty, Ali Shahcheraghi, Gregory A Denomme
    Abstract:

    The Gerbich (Ge) antigens are a collection of high-incidence antigens carried on the red blood Cell membrane glycoproteins, glycophorins C and D. Antibodies against these antigens are uncommon, and there have been only rare case reports of hemolytic disease of the fetus and newborn due to anti-Ge. In this case report, we present a neonate with severe anemia and hyperbilirubinemia due to anti-Ge3. Routine and special laboratory studies undertaken in this case suggested two mechanisms for the patient's hemolysis and persistent anemia. Antibody-dependent hemolysis was associated with early-onset hyperbilirubinemia, anemia, and a mild reticulocytosis, and inhibition of Erythroid progenitor Cell growth was associated with late anemia and normal bilirubin and reticulocyte values. Though rare, anti-Ge3 can be a dangerous antibody in pregnancy. Affected neonates may require intensive initial therapy and close follow-up for at least several weeks after delivery.

Ulrich W Schaefer - One of the best experts on this subject based on the ideXlab platform.

  • erythropoietic reconstitution macrophages and reticulin fibrosis in bone marrow specimens of cml patients following allogeneic transplantation
    Leukemia, 2000
    Co-Authors: J Thiele, Hans Michael Kvasnicka, Dietrich W Beelen, B Pilgram, A Rose, Lutzdietrich Leder, Ulrich W Schaefer
    Abstract:

    A clinicopathological study was conducted on 351 bone marrow trephine biopsies derived from 124 patients with chronic myeloid leukemia (CML) at standardized endpoints before and after allogeneic bone marrow transplantation (BMT). The purpose was to investigate quantitative changes of the nucleated Erythroid Precursor Cell population and other associated features such as resident bone marrow macrophages and myelofibrosis and to elucidate their relevance on engraftment parameters. Monoclonal antibodies were applied for the identification of Erythroid Precursors and the labeling of mature macrophages; argyrophilic (reticulin-collagen) fibers were demonstrated by a silver impregnation technique. Following morphometric analysis of the pregraft bone marrow specimens statistical evaluation was in line with an adverse correlation between early to moderate reticulin fibrosis and amount of erythropoiesis. Moreover, a significant relationship was calculable between numbers of Erythroid Precursors and CD68+ macrophages. After myelo-ablative therapy and BMT a pronounced decrease in Cellularity and in the quantity of erythropoiesis was found. Comparable with the pregraft samples, a significant association between Erythroid Precursors and macrophages could be determined in the regenerating donor bone marrow. A pretransplant relevant reduction of the red Cell lineage and a manifest (reticulin) myelofibrosis indicating an advanced stage of disease were accompanied by a significant delay to reach transfusion independence. This result was further supported by comparable findings in trephine biopsies performed in the early post-transplant period (second month after BMT). Corresponding examinations revealed an enhancement of fiber density and a decrease in erythropoiesis in those patients who did not conform with the usually accepted criteria for successful engraftment. In conclusion, compelling evidence has been produced that a significantly reduced amount of Erythroid Precursors, which is usually associated with myelofibrosis in the pretransplant bone marrow, exerts an impairment to undisturbed hematopoietic reconstitution. Moreover, a close spatial and numerical relationship between the Erythroid lineage and resident (mature) macrophages is observable, in particular in the state of regeneration after BMT.

Douglas P Blackall - One of the best experts on this subject based on the ideXlab platform.

  • hemolytic disease of the fetus and newborn due to anti ge3 combined antibody dependent hemolysis and Erythroid Precursor Cell growth inhibition
    American Journal of Perinatology, 2008
    Co-Authors: Douglas P Blackall, Gina D Pesek, Matthew M Montgomery, Krishna K Oza, Patricia A Arndt, George Garratty, Ali Shahcheraghi, Gregory A Denomme
    Abstract:

    The Gerbich (Ge) antigens are a collection of high-incidence antigens carried on the red blood Cell membrane glycoproteins, glycophorins C and D. Antibodies against these antigens are uncommon, and there have been only rare case reports of hemolytic disease of the fetus and newborn due to anti-Ge. In this case report, we present a neonate with severe anemia and hyperbilirubinemia due to anti-Ge3. Routine and special laboratory studies undertaken in this case suggested two mechanisms for the patient's hemolysis and persistent anemia. Antibody-dependent hemolysis was associated with early-onset hyperbilirubinemia, anemia, and a mild reticulocytosis, and inhibition of Erythroid progenitor Cell growth was associated with late anemia and normal bilirubin and reticulocyte values. Though rare, anti-Ge3 can be a dangerous antibody in pregnancy. Affected neonates may require intensive initial therapy and close follow-up for at least several weeks after delivery.