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Hiroshi Tsunekawa - One of the best experts on this subject based on the ideXlab platform.
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Cloning, sequencing, and expression of cscA invertase from <B>EscherichiaB> <B>ColiB> B-62.
Canadian Journal of Microbiology, 1999Co-Authors: Miklós Sahin-tóth, Zsolt Lengyel, Hiroshi TsunekawaAbstract:We have isolated a 2.5-kB DNA fragment from plasmid pST5R7 encoding a sucrose utilization system from <B>EscherichiaB> <B>ColiB> B-62 which confers a sucrose-fermenting phenotype to transformed E. <B>ColiB> K-12 strains. DNA-sequence determination revealed one full-length open reading frame 98% identical to cscA, the sucrose-hydrolase (invertase) gene of the csc regulon from E. <B>ColiB> EC3132. Functional characterization indicates that high-level expression and limited periplasmic release of invertase is responsiBle for the sucrose-fermenting capacity of transformed E. <B>ColiB> K-12 strains carrying cscA.
Hiroaki Kato - One of the best experts on this subject based on the ideXlab platform.
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<B>EscherichiaB> <B>ColiB> B gamma-glutamylcysteine synthetase: modification, purification, crystallization and preliminary crystallographic analysis.
Acta crystallographica. Section D Biological crystallography, 2002Co-Authors: Takao Hibi, Hiromoto Hisada, Toru Nakatsu, Hiroaki KatoAbstract:<B>EscherichiaB> <B>ColiB> B gamma-glutamylcysteine synthetase (gammaGCS) catalyzes the ATP-dependent coupling of L-Glu and L-Cys to form the glutathione precursor gamma-L-Glu-Cys and is a target for development of potential therapeutic agents. By introducing four point mutations of surface-exposed cysteine residues to serine, the gammaGCS was purified to homogeneity; single crystals have Been oBtained using the hanging-drop vapour-diffusion method with sodium formate. The gammaGCS crystal diffracted to 2.8 A and Belongs to space group R3, with unit-cell parameters a = B = 326.7, c = 103.9 A.
Miklós Sahin-tóth - One of the best experts on this subject based on the ideXlab platform.
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Cloning, sequencing, and expression of cscA invertase from <B>EscherichiaB> <B>ColiB> B-62.
Canadian Journal of Microbiology, 1999Co-Authors: Miklós Sahin-tóth, Zsolt Lengyel, Hiroshi TsunekawaAbstract:We have isolated a 2.5-kB DNA fragment from plasmid pST5R7 encoding a sucrose utilization system from <B>EscherichiaB> <B>ColiB> B-62 which confers a sucrose-fermenting phenotype to transformed E. <B>ColiB> K-12 strains. DNA-sequence determination revealed one full-length open reading frame 98% identical to cscA, the sucrose-hydrolase (invertase) gene of the csc regulon from E. <B>ColiB> EC3132. Functional characterization indicates that high-level expression and limited periplasmic release of invertase is responsiBle for the sucrose-fermenting capacity of transformed E. <B>ColiB> K-12 strains carrying cscA.
Takao Hibi - One of the best experts on this subject based on the ideXlab platform.
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<B>EscherichiaB> <B>ColiB> B gamma-glutamylcysteine synthetase: modification, purification, crystallization and preliminary crystallographic analysis.
Acta crystallographica. Section D Biological crystallography, 2002Co-Authors: Takao Hibi, Hiromoto Hisada, Toru Nakatsu, Hiroaki KatoAbstract:<B>EscherichiaB> <B>ColiB> B gamma-glutamylcysteine synthetase (gammaGCS) catalyzes the ATP-dependent coupling of L-Glu and L-Cys to form the glutathione precursor gamma-L-Glu-Cys and is a target for development of potential therapeutic agents. By introducing four point mutations of surface-exposed cysteine residues to serine, the gammaGCS was purified to homogeneity; single crystals have Been oBtained using the hanging-drop vapour-diffusion method with sodium formate. The gammaGCS crystal diffracted to 2.8 A and Belongs to space group R3, with unit-cell parameters a = B = 326.7, c = 103.9 A.
Zsolt Lengyel - One of the best experts on this subject based on the ideXlab platform.
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Cloning, sequencing, and expression of cscA invertase from <B>EscherichiaB> <B>ColiB> B-62.
Canadian Journal of Microbiology, 1999Co-Authors: Miklós Sahin-tóth, Zsolt Lengyel, Hiroshi TsunekawaAbstract:We have isolated a 2.5-kB DNA fragment from plasmid pST5R7 encoding a sucrose utilization system from <B>EscherichiaB> <B>ColiB> B-62 which confers a sucrose-fermenting phenotype to transformed E. <B>ColiB> K-12 strains. DNA-sequence determination revealed one full-length open reading frame 98% identical to cscA, the sucrose-hydrolase (invertase) gene of the csc regulon from E. <B>ColiB> EC3132. Functional characterization indicates that high-level expression and limited periplasmic release of invertase is responsiBle for the sucrose-fermenting capacity of transformed E. <B>ColiB> K-12 strains carrying cscA.