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Patrice A. Bulger – One of the best experts on this subject based on the ideXlab platform.

  • protection of dogs from lyme disease with a vaccine containing outer surface protein osp a ospb and the saponin adjuvant qs21
    The Journal of Infectious Diseases, 1995
    Co-Authors: Richard T. Coughlin, Durland Fish, Thomas N. Mather, Charles Pavia, Patrice A. Bulger
    Abstract:

    7. Ewing WH. The genus Escherichia. In: Edwards and Ewing’s identification of enterobacteriaceae. 4th ed. New York: Elsevier Science, 1986:93-134. 8. Svennerholm AM, Wiklund G. Rapid GM1-enzyme-linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin. J Clin Microbiol 1983;17:596-600. 9. Svennerholm AM, Wikstrom M, Lindblad M, Holmgren J. Monoclonal antibodies against Escherichia coli heat-stable toxin (ST) and their use in a diagnostic ST ganglioside GM1-enzyme-linked immunosorbent assay. J Clin Microbiol 1986;24:585-90. 10. Binsztein N, Jouve MJ, Viboud GI, et al. Colonization factors of enterotoxigenic Escherichia coli isolated from children with diarrhea in Argentina. J Clin Microbiol 1991;29:1893-8. 11. 0rskov F, 0rskov I. Serotyping of Escherichia coli. In: Bergan T, ed. Methods in microbiology. Vol 14. London: Academic Press, 1984:43-112. 12. Snedecor GW, Cochran WG. Statistical methods. 6th ed. Ames: Iowa

Huere Peña, Jorge L. – One of the best experts on this subject based on the ideXlab platform.

  • RESISTENCIA ANTIBIÓTICA DE SALMONELLA SPP, Escherichia COLI AISLADAS DE ALPACAS (Vicugna pacus) CON Y SIN DIARREA
    , 2020
    Co-Authors: Carhuapoma Dela Cruz Victo, Valencia Mamani Nicasio, Huaman Gonzales Teresa, Paucar-chanca Rufino, Hilario Lizana Epifanio, Huere Peña, Jorge L.
    Abstract:

    A nivel mundial, el problema de resistencia a antibióticos es considerado de prioridad sanitaria pública y veterinaria, por ello el objetivo de esta investigación fue evaluar la presencia de resistencia antibiótica frente a Salmonella sp., y Escherichia coli provenientes de crías de alpacas con y sin diarrea. La investigación fue de tipo descriptivo transversal múltiple. Se recolectaron 300 muestras de heces por hisopado rectal de crías de alpacas entre 10 a 60 días nacidas con y sin cuadros diarreicos provenientes de Comunidades Campesinas de Huancavelica-Perú. La presencia de Escherichia coli y Salmonella spp. Se identificó mediante pruebas bioquímicas la susceptibilidad antibacteriana por método Kirby Bauer y se evaluaron 8 antibióticos usuales del mercado veterinaria. El 100%de muestras con diarreas fueron positivas a Escherichia coli, 40,0%Salmonella spp., 20%Escherichia coli-Salmonella spp. y muestras sin diarrea 57,0%positivas a Escherichia coli, 24,0% Salmonella spp., 19.0% E. coli-Salmonella spp. Las cepas de Escherichia coli y Salmonella spp. fueron resistentes a Ampicilina (10,4 0,3), (9,3 0,2); Novomicina (11,1 0,2), (11,2 0,1); Tetraciclina (8,2 0,1), (9,2 0,3); Penicilina (9,1 0,4), (11,1 0,3); Gentamicina (10,1 0,4), (10,2 0,3) provenientes de muestras con diarrea y en muestras sin diarrea resistentes a Gentamicina (10,3 0,1), (8,2 0,1); Tetraciclina (9,2 0,4), (8,2 0,4); Ampicilina (11,2 0,1), (9,3 0,2); Penicilina (10,2 0,4), (10,1 0,3). Las cepas de Salmonella spp., y Escherichia coli aisladas de crías de alpacas con y sin diarreas evidencian resistencia antibacteriana a múltiples antibióticos usados en la veterinaria//The problem of antibiotic resistance is considered a public and veterinary sanitary priority worldwide, for that reason the aim of the study was to evaluate the presence of antibiotic resistance against Salmonella spp., Escherichia coli coming from alpaca calves with and without diarrhea. The research was cross-sectional descriptive. 300 stool samples per rectal swab were collected from alpaca calves aging from 10 and 60 days with and without diarrhea from Peasant Communities in Huancavelica – Peru. The presence of Escherichia coli and Salmonella spp. was identified by conventional biochemical test, antibacterial susceptibility by Kirby Bauer method and 8 usual antibiotics from the veterinary market were evaluated. 100% of samples with diarrhea were positive to Escherichia coli; 40.0% Salmonella spp.; 40% Escherichia coli -Salmonella spp., and samples without diarrhea 48.3% positive to Escherichia Coli; 14.0% Salmonella spp.; 9.3% Escherichia coli -Salmonella spp. The CMI in Escherichia coli and Salmonella spp. strains were resistant to Ampicillin (10.4 0.3), (9.3 0.2); Novomycin (11.1 0.2), (11.2 0. 1); Tetracycline (8.2 0.1), (9.2 0.3); Penicillin (9.1 0. 4), (11.1 0. 3); Gentamicin (10.1 0. 4), (10.2 0. 3) from samples with diarrhea and in samples without diarrhea resistant to Gentamicin (10.3 0.1), (8.2 0.1); Tetracycline (9.2 0.4), (8.2 0.4); Ampicillin (11.2 0.1), (9.3 0.2); Penicillin (10.2 0.4), (10.1 0.3). Salmonella spp., Escherichia coli strains isolated from alpaca calves with and without diarrhea show antibacterial resistance to multiple antibiotics used in veterinary

  • Resistência de Salmonella sp, Escherichia coli isoladas de alpacas (Vicugna pacus) com e sem diarreia a antibióticos
    'Salesian Polytechnic University of Ecuador', 2020
    Co-Authors: Carhuapoma De La Cruz, Vícto, Valencia Mamani Nicasio, Huaman Gonzales Teresa, Paucar-chanca Rufino, Hilario Lizana Epifanio, Huere Peña, Jorge L.
    Abstract:

    A nivel mundial, el problema de resistencia a antibióticos es considerado de prioridad sanitaria pública y veterinaria, por ello el objetivo de esta investigación fue evaluar la presencia de resistencia antibiótica frente a Salmonella sp., y Escherichia coli provenientes de crías de alpacas con y sin diarrea. La investigación fue de tipo descriptivo transversal múltiple. Se recolectaron 300 muestras de heces por hisopado rectal de crías de alpacas entre 10 a 60 días nacidas con y sin cuadros diarreicos provenientes de Comunidades Campesinas de Huancavelica-Perú. La presencia de Escherichia coli y Salmonella sp. se identificó mediante pruebas bioquímicas, la susceptibilidad antibacteriana por método Kirby Bauer y se evaluaron 8 antibióticos usuales del mercado veterinaria. El 100 % de muestras con diarreas fueron positivas a Escherichia coli, 40,0 % Salmonella sp. 20% Escherichia coli-Salmonella sp. y muestras sin diarrea 57,0% positivas a Escherichia coli, 24,0% Salmonella sp. 19.0% E. coli-Salmonella sp. Las cepas de Escherichia coli y Salmonella sp. fueron resistentes a Ampicilina (10,4± 0,3), (9,3± 0,2); Novomicina (11,1± 0,2), (11,2± 0,1); Tetraciclina (8,2± 0,1), (9,2± 0,3); Penicilina (9,1± 0,4), (11,1± 0,3); Gentamicina (10,1± 0,4), (10,2± 0,3) provenientes de muestras con diarrea y en muestras sin diarrea resistentes a Gentamicina (10,3± 0,1), (8,2± 0,1); Tetraciclina (9,2± 0,4), (8,2± 0,4); Ampicilina (11,2± 0,1), (9,3± 0,2); Penicilina (10,2± 0,4), (10,1± 0,3). Las cepas de Salmonella sp., y Escherichia coli aisladas de crías de alpacas con y sin diarreas evidencian resistencia antibacteriana a múltiples antibióticos usados en la veterinaria.The problem of antibiotic resistance, worldwide is considered a high health priority, for that reason it was evaluated the antibiotic resistance of Salmonella ssp, Escherichia Coli isolated from baby alpacas with and without diarrheal enteropathies. The investigation it was of type, multiple transversal descriptive. 300 samples of rectal swabs were collected of baby alpacas from 10 to 60 days of age born with and without diarrheal pictures of the Peasant Communities of Huancavelica-Peru. The presence of Escherichia coli and Salmonella spp was determined by conventional biochemical test, the antibacterial susceptibility by the Kirby Bauer method and 8 usual antibiotics from the veterinary market were evaluated. Baby alpacas with diarrhea were 100% positive to Escherichia coli, 40,0 % Salmonella spp ,40% E. coli -Salmonella sp and without positive diarrhea 48,3% Escherichia Coli, 14,0% Salmonella spp, 9,3% E. Coli-Salmonella spp. The CMI in strains Escherichia coli and Salmonella spp were resistant to Ampicillin (10,4± 0.3), (9,3± 0.2); Novomycin (11,1± 0. 2), (11,2± 0. 1); Tetracycline (8,2± 0.1), (9,2± 0.3); Penicillin (9,1± 0. 4), (11,1± 0. 3); Gentamicin (10,1± 0. 4), (10,2± 0. 3) isolated from baby alpaca with diarrhea and without diarrhea the CMI they were resistant to Gentamicin (10,3± 0.1), (8,2± 0.1); Tetracycline (9,2± 0.4), (8,2± 0.4); Ampicillin (11,2± 0.1), (9,3± 0.2); Penicillin (10,2± 0. 4), (10,1± 0. 3). The strains of Salmonella spp, Escherichia coli isolated from baby alpaca with and without diarrhea evidence high resistance to multiple veterinary antibiotics.A nível mundial, o problema da resistência a antibióticos é considerado uma prioridade da saúde pública e veterinária, portanto, o objetivo desta pesquisa foi avaliar a presença da resistência de Salmonella sp. e Escherichia coli proveniente de alpacas com e sem diarreia aos antibióticos. A pesquisa foi do tipo transversal descritivo múltiplo. Foram coletadas 300 amostras de fezes por swab retal de bezerros de alpaca entre 10 e 60 dias de vida com e sem quadros diarreicos, das Comunidades Camponesas de Huancavelica-Peru. A presença de Escherichia coli e Salmonella sp foram identificadas com base em teste bioquímicos, a suscetibilidade antibacteriana pelo método Kirby Bauer e foram avaliados 8 tipos de antibióticos comumente utilizados no mercado veterinário. 100% das amostras com diarreia foram positivas para Escherichia coli, 40,0% a Salmonella sp., 20% Escherichia coli-Salmonella sp. e das amostras sem diarreia, 57,0% foram positivas para Escherichia coli, 24,0% para Salmonella sp. e 19,0% para E. coli-Salmonella sp. As cepas de Escherichia coli e Salmonella sp. nas amostras com diarreia foram resistentes à ampicilina (10,4 ± 0,3), (9,3 ± 0,2); Novomicina (11,1 ± 0,2), (11,2 ± 0,1); Tetraciclina (8,2 ± 0,1), (9,2 ± 0,3); Penicilina (9,1 ± 0,4), (11,1 ± 0,3) e Gentamicina (10,1 ± 0,4), (10,2 ± 0,3), respectivamente, e das amostras sem diarreia foram resistentes à Gentamicina (10,3 ± 0,1) (8,2 ± 0, 1); Tetraciclina (9,2 ± 0,4), (8,2 ± 0,4); Ampicilina (11,2 ± 0,1), (9,3 ± 0,2); Penicilina (10,2 ± 0,4), (10,1 ± 0,3), respectivamente. As linhagens de Salmonella sp, e Escherichia coli isoladas de bezerros de alpaca com e sem diarreia apresentam resistência antibacteriana a múltiplos antibióticos utilizados na veterinária

Dai Ding-zhen – One of the best experts on this subject based on the ideXlab platform.

  • Comparison of Homology of Type P Pili between Avian Escherichia coli and Human Escherichia coli
    Journal of Jinling Institute of Technology, 2007
    Co-Authors: Dai Ding-zhen
    Abstract:

    With the help of Goldkey programs,homology of papA gene between human Escherichia coli and avian Escherichia coli was compared and analysed.Similarity of papA gene and amino acid sequence encoded by papA are 82.5% and 83.7% respectively,and the signal sequence of the human Escherichia coli is the same as that of avian Escherichia coli.Similar secondary structure exists between them.

  • Immunogenicity of type 1 pili vaccine of avian Escherichia coli
    Jiangsu Journal of Agricultural Sciences, 2005
    Co-Authors: Gan Liming, Wang Xiaoli, Liu Hongzhen, Xia Xingxia, Dai Ding-zhen, Qin Aijian
    Abstract:

    Avian Escherichia coli pathogenic strains M10(O11)、GX7(O78) and YR10(O18) with type 1 pili were cultured in nutrient broth at 37 for 48 hours respectively,the pili from O18(YR10) was extracted and used for preparation of multivalent oil-emulsion vaccine.Both O18 Escherichia coli type 1 pili vaccine and the multivalent pili oil-emulsion vaccine of Escherichia coli(O11-O78-O18) were made successfully.Four week-old SPF chickens were vaccinated with Escherichia coli O18 pili vaccine(200 μg for every chicken) and Escherichia coli O18 with pili vaccine subcutaneously.After three weeks unvaccinated chickens suffered 66.67%—88.88% mortality after challenged via the posterior thoracic air sac with Escherichia coli,while vaccinated chickens suffered 6.25%—41.17% mortality when challenged with heterogenous Escherichia coli(YR10).Four-weer-old SPF chickens were vaccinated with Escherichia coli O11-O78-O18 multivalent pili oil-emulsion inactivated vaccine,vaccinate chickens suffered 13.33% mortality,unvaccinated chickens suffered 70.00% mortality after challenged with homologous Escherichia coli and 61.53% mortality when challenged with heterologous(Escherichi-) a coli(O2),but vaccinated chickens suffered 3.33% mortality when challenged with Escherichia coli(O2),The results showed that the multivalent pili oil-emulsion vaccine had good cross protection effect against avain E.coli.