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José Luis - One of the best experts on this subject based on the ideXlab platform.
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Esculin and its oligomer fractions inhibit adhesion and migration of u87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2016Co-Authors: Imen Mokdadbzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekirghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their respective receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.
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Esculin and its oligomer fractions inhibit adhesion and migration of U87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2015Co-Authors: Imen Mokdad-bzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekir-ghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their resp. receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.
Kamel Ghedira - One of the best experts on this subject based on the ideXlab platform.
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genotoxic and anti genotoxic effects of Esculin and its oligomer fractions against mitomycin c induced dna damages in mice
Regulatory Toxicology and Pharmacology, 2016Co-Authors: Imen Mokdad Bzeouich, Kamel Ghedira, Mohamed Ghoul, Nadia Mustapha, Mouna Maatouk, Leila ChekirghediraAbstract:Mitomycin C is one of the most effective chemotherapeutic drugs against various solid tumors. However, despite its wide spectrum of clinical benefits, this agent is capable of inducing various types of genotoxicity. In this study, we investigated the effect of Esculin and its oligomer fractions (E1, E2 and E3) against mitomycin C induced genotoxicity in liver and kidney cells isolated from Balb/C mice using the comet assay. Esculin and its oligomer fractions were not genotoxic at the tested doses (20 mg/kg and 40 mg/kg b.w). A significant decrease in DNA damages was observed, suggesting a protective role of Esculin and its oligomer fractions against the genotoxicity induced by mitomycin C on liver and kidney cells. Moreover, Esculin and its oligomer fractions did not induce an increase of malondialdehyde levels.
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Esculin and its oligomer fractions inhibit adhesion and migration of u87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2016Co-Authors: Imen Mokdadbzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekirghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their respective receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.
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Oligomerization of Esculin improves its antibacterial activity and modulates antibiotic resistance
The Journal of Antibiotics, 2015Co-Authors: Imen Mokdad-bzeouich, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Nadia Mustapha, Fadwa Chaabane, Zied Ghedira, Leila Chekir-ghediraAbstract:In this particular study, the antibacterial activity of Esculin and oligomer fractions was assessed. MIC values of Esculin and its oligomer fractions as well as of some antibiotics against Gram-positive and Gram-negative strains and against Escherichia coli multiresistant variants were determined by the standard broth microdilution method. Both Esculin and oligoEsculin fractions exhibited antibacterial effect against reference strains; Staphylococcus aureus , Enterococcus faecalis , Salmonella enteritidis and Salmonella typhimurium . It appears that E3 oligomer fraction had the greatest antibacterial activity against these reference strains. Besides, as E2 and E3 revealed the best antibacterial effect against multiresistant variants of E. coli , we decided to test the effect of each, combined to the antibiotic against which the variants were resistant. In the interaction study, E2 and E3 oligoEsculin fractions were found to be effective in reducing the resistance of E. coli 6574 to ofloxacin and the resistance of E. coli 6228 to amoxicillin. Only E3 oligoEsculin fraction showed a synergetic interaction with amoxicillin and tetracyclin against E. coli 6708, but no interaction was found either with E2 or E3 fractions against E. coli 6234. Our study allowed us to conclude that oligomerization of Esculin increases its antibacterial potential, according to the degree of polymerization.
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Esculin and its oligomer fractions inhibit adhesion and migration of U87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2015Co-Authors: Imen Mokdad-bzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekir-ghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their resp. receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.
Mohamed Ghoul - One of the best experts on this subject based on the ideXlab platform.
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genotoxic and anti genotoxic effects of Esculin and its oligomer fractions against mitomycin c induced dna damages in mice
Regulatory Toxicology and Pharmacology, 2016Co-Authors: Imen Mokdad Bzeouich, Kamel Ghedira, Mohamed Ghoul, Nadia Mustapha, Mouna Maatouk, Leila ChekirghediraAbstract:Mitomycin C is one of the most effective chemotherapeutic drugs against various solid tumors. However, despite its wide spectrum of clinical benefits, this agent is capable of inducing various types of genotoxicity. In this study, we investigated the effect of Esculin and its oligomer fractions (E1, E2 and E3) against mitomycin C induced genotoxicity in liver and kidney cells isolated from Balb/C mice using the comet assay. Esculin and its oligomer fractions were not genotoxic at the tested doses (20 mg/kg and 40 mg/kg b.w). A significant decrease in DNA damages was observed, suggesting a protective role of Esculin and its oligomer fractions against the genotoxicity induced by mitomycin C on liver and kidney cells. Moreover, Esculin and its oligomer fractions did not induce an increase of malondialdehyde levels.
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Esculin and its oligomer fractions inhibit adhesion and migration of u87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2016Co-Authors: Imen Mokdadbzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekirghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their respective receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.
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Oligomerization of Esculin improves its antibacterial activity and modulates antibiotic resistance
The Journal of Antibiotics, 2015Co-Authors: Imen Mokdad-bzeouich, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Nadia Mustapha, Fadwa Chaabane, Zied Ghedira, Leila Chekir-ghediraAbstract:In this particular study, the antibacterial activity of Esculin and oligomer fractions was assessed. MIC values of Esculin and its oligomer fractions as well as of some antibiotics against Gram-positive and Gram-negative strains and against Escherichia coli multiresistant variants were determined by the standard broth microdilution method. Both Esculin and oligoEsculin fractions exhibited antibacterial effect against reference strains; Staphylococcus aureus , Enterococcus faecalis , Salmonella enteritidis and Salmonella typhimurium . It appears that E3 oligomer fraction had the greatest antibacterial activity against these reference strains. Besides, as E2 and E3 revealed the best antibacterial effect against multiresistant variants of E. coli , we decided to test the effect of each, combined to the antibiotic against which the variants were resistant. In the interaction study, E2 and E3 oligoEsculin fractions were found to be effective in reducing the resistance of E. coli 6574 to ofloxacin and the resistance of E. coli 6228 to amoxicillin. Only E3 oligoEsculin fraction showed a synergetic interaction with amoxicillin and tetracyclin against E. coli 6708, but no interaction was found either with E2 or E3 fractions against E. coli 6234. Our study allowed us to conclude that oligomerization of Esculin increases its antibacterial potential, according to the degree of polymerization.
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Esculin and its oligomer fractions inhibit adhesion and migration of U87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2015Co-Authors: Imen Mokdad-bzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekir-ghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their resp. receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.
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Effect of acyl donor chain length and substitutions pattern on the enzymatic acylation of flavonoids
Journal of Biotechnology, 2004Co-Authors: M. Ardhaoui, A. Falcimaigne, S Ognier, J.m. Engasser, P. Moussou, G. Pauly, Mohamed GhoulAbstract:Abstract Rutin and Esculin were enzymatically acylated with different aliphatic acids as acyl donors (fatty acids, dicarboxylic acids and ω-substituted fatty acids) by an immobilized lipase from Candida antarctica. The effect of the water content and the acyl donors pattern on the flavonoid initial acylation rate and conversion yield were investigated. The obtained results indicated that the water content of the medium has a strong effect on the performance of these reactions. The best conversion yields were reached when the water content was kept lower than 200 ppm. At low water content of the medium, these syntheses are influenced by carbon chain length and substitution pattern of the acyl donors. Higher conversion yields of Esculin and rutin (>70%) were obtained with aliphatic acids having high carbon chain length (>12). Moreover, it has been found that the amine and thiol groups on ω-substituted fatty acid chain were unfavourable to these reactions. The 1 H NMR and 13 C NMR analyses of some synthesized esters (Esculin and rutin palmitate) show that only monoesters were produced and that the esterification takes place on the primary OH of glucose moiety of the Esculin and on the secondary 4′′′-OH of the rhamnose residue of rutin.
Dalong Zhao - One of the best experts on this subject based on the ideXlab platform.
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anti apoptotic effect of Esculin on dopamine induced cytotoxicity in the human neuroblastoma sh sy5y cell line
Neuropharmacology, 2007Co-Authors: Dalong Zhao, Libo Zou, Sheng Lin, Jiangong Shi, Haibo ZhuAbstract:Abstract Dopamine (DA), as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. In this study, we examined the effect of Esculin, which was extracted from Fraxinus sielboldiana blume, on DA-induced cytotoxicity and the underlying mechanism in human neuroblastoma SH-SY5Y cells. Our results suggest that the protective effects of Esculin (10 −7 , 10 −6 and 10 −5 M) on DA-induced cytotoxicity may be ascribed to its anti-oxidative properties by reducing ROS level, and its anti-apoptotic effect via protecting mitochondrion membrane potential (ΔΨm), enhancing superoxide dismutaese (SOD) activity and reduced glutathione (GSH) levels, and regulating P53, Bax and Bcl-2 expression. In addition, Esculin inhibited the release of cytochrome c and apoptosis-inducing factor (AIF), and the protein expression of activated caspase 3. These data indicate that Esculin may provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease (PD).
Hervé Kovacic - One of the best experts on this subject based on the ideXlab platform.
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Esculin and its oligomer fractions inhibit adhesion and migration of u87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2016Co-Authors: Imen Mokdadbzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekirghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their respective receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.
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Esculin and its oligomer fractions inhibit adhesion and migration of U87 glioblastoma cells and in vitro angiogenesis
Tumor Biology, 2015Co-Authors: Imen Mokdad-bzeouich, Hervé Kovacic, Kamel Ghedira, Latifa Chebil, Mohamed Ghoul, Leila Chekir-ghedira, José LuisAbstract:Cancer metastasis is the major cause of cancer-related death. Chemoprevention is defined as the use of natural or synthetic substances to prevent cancer formation or cancer progress. In the present study, we investigate the antitumor activity of Esculin and its oligomer fractions in U87 glioblastoma cells. We showed that Esculin and its oligomers reduced U87 cell growth in a dose dependent manner. They also inhibited cell adhesion to collagen IV and vitronectin by interfering with the function of their resp. receptors α2β1 and αvβ5 integrins. Furthermore, the tested samples were able to reduce migration of U87 cells towards another extracellular matrix fibronectin. Moreover, Esculin and its oligomer fractions inhibited in vitro angiogenesis of endothelial cells (HMEC-1). In summary, our data provide the first evidence that Esculin and its oligomer fractions are able to reduce adhesion, migration of glioblastoma cells and in vitro angiogenesis. Esculin and its oligomers may thus exert multi-target functions against cancer cells.