The Experts below are selected from a list of 159 Experts worldwide ranked by ideXlab platform

Martin Novotný - One of the best experts on this subject based on the ideXlab platform.

  • Pre- and/or Postsurgical Administration of Estradiol Benzoate Increases Skin Flap Viability in Female Rats
    Aesthetic plastic surgery, 2013
    Co-Authors: Tomáš Vasilenko, Martin Slezák, Martin Novotný, Ivan Kováč, Ján Ďurkáč, Iveta Tomková, Norbert Torma, Andrej Vrzgula, Ľ. Lenhardt, Mikukáš Levkut
    Abstract:

    Background It has been shown that estrogens have a protective effect with regard to tissue ischemia. Therefore, in this macroscopic and histological investigation, the effect of Estradiol Benzoate on skin flap viability was studied in sham-operated and ovariectomized Sprague-Dawley rats.

  • pre and or postsurgical administration of Estradiol Benzoate increases skin flap viability in female rats
    Aesthetic Plastic Surgery, 2013
    Co-Authors: Tomáš Vasilenko, Martin Slezák, Martin Novotný, Ivan Kováč, Iveta Tomková, Norbert Torma, Andrej Vrzgula, Jan ďurkac, Ľ. Lenhardt
    Abstract:

    Background It has been shown that estrogens have a protective effect with regard to tissue ischemia. Therefore, in this macroscopic and histological investigation, the effect of Estradiol Benzoate on skin flap viability was studied in sham-operated and ovariectomized Sprague-Dawley rats.

  • postsurgical administration of Estradiol Benzoate decreases tensile strength of healing skin wounds in ovariectomized rats
    Journal of Surgical Research, 2008
    Co-Authors: Petr Gal, Martin Novotný, Tomas Toporcer, B Vidinský, Michal Mokrý, Tomas Grendel, Jan Sokolský, Nikita Bobrov, Silvia Toporcerova, Jan Sabo
    Abstract:

    Background The most effective method of increasing the level of estrogen in the wounds of post-menopausal women undergoing routine surgical procedures is by long-term preoperative administration. However, in the case of acute surgery or trauma, the most effective method of increasing the level of estrogen is administration immediately pre- or postsurgery. This study, therefore, was aimed at assessing the effect of postsurgical administration of Estradiol Benzoate on wound healing in ovariectomized (OVX) Sprague Dawley rats. Materials and methods Three months prior to the wound healing experiment, 16 rats were anesthetized and underwent ovariectomy, while the other eight rats were sham operated. Two parallel full thickness skin incisions and two round full thickness skin excisions were performed on the dorsum of each rat. Dose of 10 μg/d of Estradiol Benzoate was administered to eight OVX rats for 6 d postoperatively, whereas the other animals received a placebo. After 6 d, all animals were sacrificed and samples removed for biomechanical and histological evaluation. Results The mean wound tensile strength of OVX estrogen treated rats (9.54 ± 3.24 g/mm2) was significantly lower compared with vehicle-treated OVX animals (14.57 ± 4.12 g/mm2) as well as with control rats subjected to sham-OVX surgery (11.71 ± 3.33 g/mm2). Nevertheless, the histological evaluation in OVX estrogen treated rats showed a significantly increased process of neo-angiogenesis associated with slightly decreased collagen deposition. Conclusion Our results indicate that the question of the clinical significance of this type of hormone replacement therapy remains open and requires further research.

T L Thompson - One of the best experts on this subject based on the ideXlab platform.

  • Attenuation of dopamine uptake in vivo following priming with Estradiol Benzoate.
    Brain research, 1999
    Co-Authors: T L Thompson
    Abstract:

    Dopamine (DA) uptake and clearance were examined using in vivo voltammetry following injection of DA (200 microM) into the nucleus accumbens of ovariectomized (OVX) or OVX-estrogen-primed rats (Estradiol Benzoate, EB, 10 microg 48 and 24 h prior to experiment). The rate of DA uptake was significantly attenuated in steroid-treated animals: this decrease was accompanied by a significant increase in DA clearance time. Quinpirole (0.5 mg/kg) modulated the kinetics of DA uptake in OVX but not EB-primed rats. These data suggest that DA clearance can be regulated by physiological doses of EB.

Mirjam Nielen - One of the best experts on this subject based on the ideXlab platform.

  • liquid chromatography electrospray ionisation mass spectrometry based method for the determination of Estradiol Benzoate in hair of cattle
    Analytica Chimica Acta, 2005
    Co-Authors: H. Hooijerink, Arjen Lommen, Patrick P.j. Mulder, J.a. Van Rhijn, Mirjam Nielen
    Abstract:

    Abstract A liquid chromatography (LC)-based method with mass spectrometric (MS/MS) detection was developed for the determination of Estradiol Benzoate residues in hair of cattle. First hair samples were pulverized with a ball mill followed by treatment with the reducing agent Tris(2-carboxyethyl)phosphine hydrochloride (TCEP). After liquid/liquid extraction samples were further purified by solid-phase extraction. Finally samples were analyzed with LC–MS/MS using deuterated Estradiol Benzoate as internal standard. The method was validated following the latest EU guidelines for screening methods using blank hair samples spiked at 5 ng g−1. The detection capability (CCβ) was less than 5 ng g−1 and the decision limit (CCα) was 1.6 ng g−1. The recovery was 27% at the 5 ng g−1 level and the accuracy was 95%. Confirmation, with two MS/MS transitions, was possible at a concentration of 5 ng g−1 or higher. Incurred samples obtained from different animal experiments were analyzed and the presence of Estradiol Benzoate was confirmed. Finally hair samples from different slaughterhouses were analyzed.

  • Liquid chromatography–electrospray ionisation-mass spectrometry based method for the determination of Estradiol Benzoate in hair of cattle
    Analytica Chimica Acta, 2004
    Co-Authors: H. Hooijerink, Arjen Lommen, Patrick P.j. Mulder, J.a. Van Rhijn, Mirjam Nielen
    Abstract:

    Abstract A liquid chromatography (LC)-based method with mass spectrometric (MS/MS) detection was developed for the determination of Estradiol Benzoate residues in hair of cattle. First hair samples were pulverized with a ball mill followed by treatment with the reducing agent Tris(2-carboxyethyl)phosphine hydrochloride (TCEP). After liquid/liquid extraction samples were further purified by solid-phase extraction. Finally samples were analyzed with LC–MS/MS using deuterated Estradiol Benzoate as internal standard. The method was validated following the latest EU guidelines for screening methods using blank hair samples spiked at 5 ng g−1. The detection capability (CCβ) was less than 5 ng g−1 and the decision limit (CCα) was 1.6 ng g−1. The recovery was 27% at the 5 ng g−1 level and the accuracy was 95%. Confirmation, with two MS/MS transitions, was possible at a concentration of 5 ng g−1 or higher. Incurred samples obtained from different animal experiments were analyzed and the presence of Estradiol Benzoate was confirmed. Finally hair samples from different slaughterhouses were analyzed.

Tomáš Vasilenko - One of the best experts on this subject based on the ideXlab platform.

Ľ. Lenhardt - One of the best experts on this subject based on the ideXlab platform.