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George Eng - One of the best experts on this subject based on the ideXlab platform.

Adriano A Bordalo - One of the best experts on this subject based on the ideXlab platform.

  • crenarchaeota and euryarchaeota in temperate Estuarine Sediments
    Journal of Applied Microbiology, 2001
    Co-Authors: C Abreu, G Jurgens, P De Marco, A Saano, Adriano A Bordalo
    Abstract:

    AIMS: Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large variety of ecosystems. The aim was therefore to assess the presence and diversity of Archaea in the Sediments of the river Douro estuary (Portugal), relating the results obtained to ecological data. METHODS AND RESULTS: Total DNA was extracted from sediment samples obtained from an estuary deprived of vegetation, amplified by PCR and the resulting DNA fragments cloned. The archaeal origin of the cloned inserts was checked by Southern blot, dot blot or colony blot hybridization. Recombinant plasmids were further analysed by restriction with AvaII and selected for sequencing. Phylogenetic analyses of 14 sequences revealed the presence of members of the domain Archaea. Most of the sequences could be assigned to the kingdom Crenarchaeota. CONCLUSION: Most of these sequences were closely related to those obtained from non-extreme Crenarchaeota members previously retrieved from diverse ecosystems, such as freshwater and marine environments. SIGNIFICANCE AND IMPACT OF THE STUDY: The presence of archaeal 16S rDNA sequences in temperate Estuarine Sediments emerges as a valuable contribution to the understanding of the complexity of the ecosystem.

  • crenarchaeota and euryarchaeota in temperate Estuarine Sediments
    Journal of Applied Microbiology, 2001
    Co-Authors: C Abreu, G Jurgens, P De Marco, A Saano, Adriano A Bordalo
    Abstract:

    C . A B R E U , G . J U R G E N S , P . D E M A R C O , A . S A A N O A N D A . A . B O R D A L O . 2001. Aims: Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large variety of ecosystems. The aim was therefore to assess the presence and diversity of Archaea in the Sediments of the river Douro estuary (Portugal), relating the results obtained to ecological data. Methods and Results: Total DNA was extracted from sediment samples obtained from an estuary deprived of vegetation, amplified by PCR and the resulting DNA fragments cloned. The archaeal origin of the cloned inserts was checked by Southern blot, dot blot or colony blot hybridization. Recombinant plasmids were further analysed by restriction with AvaII and selected for sequencing. Phylogenetic analyses of 14 sequences revealed the presence of members of the domain Archaea. Most of the sequences could be assigned to the kingdom Crenarchaeota. Conclusions: Most of these sequences were closely related to those obtained from nonextreme Crenarchaeota members previously retrieved from diverse ecosystems, such as freshwater and marine environments. Significance and Impact of the Study: The presence of archaeal 16S rDNA sequences in temperate Estuarine Sediments emerges as a valuable contribution to the understanding of the complexity of the ecosystem.

Rosemarie A. Lucero - One of the best experts on this subject based on the ideXlab platform.

Leopold May - One of the best experts on this subject based on the ideXlab platform.

M. Glory - One of the best experts on this subject based on the ideXlab platform.

  • Trace element contamination in the Estuarine Sediments along Tuticorin coast--Gulf of Mannar, southeast coast of India.
    Marine pollution bulletin, 2013
    Co-Authors: N. S. Magesh, N. Chandrasekar, S. Krishna Kumar, M. Glory
    Abstract:

    Sediment samples were collected from Kallar, Korampallam creek and Punnakayal estuaries of Tuticorin coast for assessing the level of contamination by trace elements in these Estuarine Sediments. The trace element concentration, calcium carbonate, organic carbon and mercury level were analyzed by Inductively Coupled Plasma Atomic Emission Spectrophotometer, Titrimetric method and SnCl2 reduction method. The results reveals that the enrichment factor, metal pollution index and geo-accumulation index of these Estuarine Sediments were predominantly polluted by Cd, As, Zn, Hg and Pb. The factor analysis revealed the source of trace element accumulation in the Estuarine Sediments particularly Mn and Fe are from riverine inputs and As and Hg from untreated industrial effluents. Among the selected estuaries, Korampallam creek was found to be highly contaminated by trace elements due to the discharge of effluents from thermal power plant, Tuticorin alkali chemicals, copper smelting, Petrochemical industries and shipping activities.