The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
Banasri Hazra - One of the best experts on this subject based on the ideXlab platform.
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Apoptosis-like death in Leishmania donovani promastigotes induced by diospyrin and its Ethanolamine Derivative.
International journal of antimicrobial agents, 2009Co-Authors: Piyali Mukherjee, Subhalakshmi Ghosh, Sutapa Biswas Majee, Banasri HazraAbstract:Abstract Previous studies have demonstrated that diospyrin ( 1 ), a quinonoid plant product, can inhibit the growth of Leishmania donovani parasites. Here, several Derivatives of 1 were evaluated by the MTT assay and it was observed that the Ethanolamine analogue ( 10 ) exhibited maximum cytotoxicity [50% inhibitory concentration (IC 50 ) = 2.9 μM] against L. donovani promastigotes. Subsequently, the mode of cell death in promastigotes was investigated through externalisation of membrane-associated phosphatidylserine, mitochondrial membrane depolarisation, DNA laddering and in situ labelling of DNA fragmentation by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) methods. Whilst both 1 and 10 were found to induce apoptosis-like death in promastigotes, the effect of 10 was evidently stronger even at a lower concentration. Hence, the Ethanolamine Derivative ( 10 ) of diospyrin ( 1 ) may be a prospective ‘lead’ for the development of novel cytotoxic agents inducing apoptosis in L. donovani parasites.
Sanggi Paik - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of a phosphatidyl Ethanolamine Derivative on lps induced sepsis
Molecules and Cells, 2009Co-Authors: Chunghyun Lee, Jungln Kim, Hayyoung Lee, Sanggi PaikAbstract:Sepsis is the leading cause of death in critically ill patients. Today, around 60% of all cases of sepsis are caused by Gram-negative bacteria. The cell wall component lipopoly-saccharide (LPS) is the main initiator of the cascade of cellular reactions in Gram-negative infections. The core receptors for LPS are toll-like receptor 4 (TLR4), MD-2 and CD14. Attempts have been made to antagonize the toxic effect of endotoxin using monoclonal antibodies against CD14 and synthetic lipopolysaccharides but there is as yet no effective treatment for septic syndrome. Here, we describe an inhibitory effect of a phosphatidylEthanolamine Derivative, PE-DTPA (phosphatidylEthanolamine diethyl-enetriaminepentaacetate) on LPS recognition. PE-DTPA bound strongly to CD14 (K d , 9.52 × 10−8 M). It dose dependency inhibited LPS-mediated activation of human myeloid cells, mouse macrophage cells and human whole blood as measured by the production of tumor necrosis factor-a (TNF-α) and nitric oxide, whereas other phospho-lipids including phosphatidylserine and phosphatidylEthanolamine had little effect. PE-DTPA also inhibited transcription dependent on NF-κB activation when it was added together with LPS, and it rescued LPS-primed mice from septic death. These results suggest that PE-DTPA is a potent antagonist of LPS, and that it acts by competing for binding to CD14.
Piyali Mukherjee - One of the best experts on this subject based on the ideXlab platform.
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Apoptosis-like death in Leishmania donovani promastigotes induced by diospyrin and its Ethanolamine Derivative.
International journal of antimicrobial agents, 2009Co-Authors: Piyali Mukherjee, Subhalakshmi Ghosh, Sutapa Biswas Majee, Banasri HazraAbstract:Abstract Previous studies have demonstrated that diospyrin ( 1 ), a quinonoid plant product, can inhibit the growth of Leishmania donovani parasites. Here, several Derivatives of 1 were evaluated by the MTT assay and it was observed that the Ethanolamine analogue ( 10 ) exhibited maximum cytotoxicity [50% inhibitory concentration (IC 50 ) = 2.9 μM] against L. donovani promastigotes. Subsequently, the mode of cell death in promastigotes was investigated through externalisation of membrane-associated phosphatidylserine, mitochondrial membrane depolarisation, DNA laddering and in situ labelling of DNA fragmentation by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) methods. Whilst both 1 and 10 were found to induce apoptosis-like death in promastigotes, the effect of 10 was evidently stronger even at a lower concentration. Hence, the Ethanolamine Derivative ( 10 ) of diospyrin ( 1 ) may be a prospective ‘lead’ for the development of novel cytotoxic agents inducing apoptosis in L. donovani parasites.
Chunghyun Lee - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of a phosphatidyl Ethanolamine Derivative on lps induced sepsis
Molecules and Cells, 2009Co-Authors: Chunghyun Lee, Jungln Kim, Hayyoung Lee, Sanggi PaikAbstract:Sepsis is the leading cause of death in critically ill patients. Today, around 60% of all cases of sepsis are caused by Gram-negative bacteria. The cell wall component lipopoly-saccharide (LPS) is the main initiator of the cascade of cellular reactions in Gram-negative infections. The core receptors for LPS are toll-like receptor 4 (TLR4), MD-2 and CD14. Attempts have been made to antagonize the toxic effect of endotoxin using monoclonal antibodies against CD14 and synthetic lipopolysaccharides but there is as yet no effective treatment for septic syndrome. Here, we describe an inhibitory effect of a phosphatidylEthanolamine Derivative, PE-DTPA (phosphatidylEthanolamine diethyl-enetriaminepentaacetate) on LPS recognition. PE-DTPA bound strongly to CD14 (K d , 9.52 × 10−8 M). It dose dependency inhibited LPS-mediated activation of human myeloid cells, mouse macrophage cells and human whole blood as measured by the production of tumor necrosis factor-a (TNF-α) and nitric oxide, whereas other phospho-lipids including phosphatidylserine and phosphatidylEthanolamine had little effect. PE-DTPA also inhibited transcription dependent on NF-κB activation when it was added together with LPS, and it rescued LPS-primed mice from septic death. These results suggest that PE-DTPA is a potent antagonist of LPS, and that it acts by competing for binding to CD14.
Sutapa Biswas Majee - One of the best experts on this subject based on the ideXlab platform.
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Apoptosis-like death in Leishmania donovani promastigotes induced by diospyrin and its Ethanolamine Derivative.
International journal of antimicrobial agents, 2009Co-Authors: Piyali Mukherjee, Subhalakshmi Ghosh, Sutapa Biswas Majee, Banasri HazraAbstract:Abstract Previous studies have demonstrated that diospyrin ( 1 ), a quinonoid plant product, can inhibit the growth of Leishmania donovani parasites. Here, several Derivatives of 1 were evaluated by the MTT assay and it was observed that the Ethanolamine analogue ( 10 ) exhibited maximum cytotoxicity [50% inhibitory concentration (IC 50 ) = 2.9 μM] against L. donovani promastigotes. Subsequently, the mode of cell death in promastigotes was investigated through externalisation of membrane-associated phosphatidylserine, mitochondrial membrane depolarisation, DNA laddering and in situ labelling of DNA fragmentation by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) methods. Whilst both 1 and 10 were found to induce apoptosis-like death in promastigotes, the effect of 10 was evidently stronger even at a lower concentration. Hence, the Ethanolamine Derivative ( 10 ) of diospyrin ( 1 ) may be a prospective ‘lead’ for the development of novel cytotoxic agents inducing apoptosis in L. donovani parasites.