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Liu Cheng-jun - One of the best experts on this subject based on the ideXlab platform.

  • CONSTRUCTION AND IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS Rv3873 FROM RD1 Eukaryotic Expression Vector
    Modern Preventive Medicine, 2007
    Co-Authors: Liu Cheng-jun
    Abstract:

    [Objective]To construct and identify the Eukaryotic Expression Vector of Mycobacterium tuberculosis Rv3873. [Methods]Rv3873 was amplified by PCR and directly cloned into Expression plasmid pGEX-4T-1 for sequencing. The target gene in the recombinant plasmid(pGEX-4T-1-Rv3873)was sub-cloned into the Eukaryotic Expression Vector pcDNA3.1(+)and the recombinant pcDNA3.1-Rv3873. This recombinant plasmid was identified by restriction analysis and PCR.[Results]Comparison of the sequence of Rv3873 gene with that reported in GenBank showed that the identities were 100%.And it was comfirmed that Rv3873 gene was successfully inserted into pcDNA3.1(+).[Conclusion]We successfully construct the Eukaryotic Expression Vector pcDNA3.1-Rv3873 and it lays a good foundation for the research of new DNA vaccine of Mycobacterium tubereulosis.

Zhang Yu-xiang - One of the best experts on this subject based on the ideXlab platform.

  • Construction and Identification of the Eukaryotic Expression Vector of Human Notch1 Transmembrane Domain
    Journal of Capital Medical University, 2009
    Co-Authors: Zhang Yu-xiang
    Abstract:

    Objective To construct the Eukaryotic Expression Vector of human Notch1 transmembrane domain(NTM), pcDNA3-NTM, and to examine its Expression in vitro. Methods Total RNA was extracted from the BxPC3 cells and reverse transcription-polymerase chain reaction(RT-PCR) was performed to obtain the cDNA fragment encoding Notch1 transmembrane domain, which was inserted into the pcDNA3 Vector in-frame with the flag sequence. And the new construct was confirmed by restriction enzyme digestion and DNA sequencing. HeLa cells were transfected with the pcDNA3-NTM Vector and pcDNA3 Vector, respectively. The Expression of NTM was detected by Western blotting. Results The Eukaryotic Expression Vector pcDNA3-NTM was constructed, and significant increase of NTM Expression was detected in the HeLa cells 48 hours after transfection. Conclusion The Eukaryotic Expression Vector pcDNA3-NTM was constructed successfully.

Niu Xinhua - One of the best experts on this subject based on the ideXlab platform.

  • Construction and identification of Eukaryotic Expression Vector for human acidic fibroblast growth factor
    Journal of Binzhou Medical University, 2011
    Co-Authors: Niu Xinhua
    Abstract:

    Objective To construct and identify Eukaryotic Expression Vector for human acidic fibroblast growth factor(haFGF).Methods The haFGF gene was amplified by PCR,digested by BglⅡ and HindⅢ,then the fragment was cloned into Eukaryotic Expression Vector pEGFP-N1.The correctness of recombinant plasmid was identified by restriction endonuclease digesting and DNA sequencing.Results The Eukaryotic Expression Vector for haFGF was digested by Bgl Ⅱ and Hind Ⅲ,and the electrophoresis of the digested products showed two fragments:4 700 bp fragment and 465 bp fragment.The result of DNA sequencing analysis was consistent with the sequence published in GeneBank.Conclusion The Eukaryotic Expression Vector for human acidic fibroblast growth factor pEGFP-N1-haFGF has been constructed and it will establish the base for studying its function and activity in future.

Zhao Guoqiang - One of the best experts on this subject based on the ideXlab platform.

Bao Yuzho - One of the best experts on this subject based on the ideXlab platform.