The Experts below are selected from a list of 1854 Experts worldwide ranked by ideXlab platform
Takaya Moriguchi - One of the best experts on this subject based on the ideXlab platform.
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Identification of differentially-expressed genes potentially related to stress tolerance in a transgenic line of European Pear over-expressing an apple spermidine synthase gene (MdSPDS1)
The Journal of Horticultural Science and Biotechnology, 2011Co-Authors: Xiao-peng Wen, Yusuke Ban, Xiaoming Pang, Takaya MoriguchiAbstract:SummaryPolyamines confer abiotic stress tolerance through mechanisms that are not yet fully understood. Previous studies on a transgenic line of European Pear (Pyrus communis L., line #32), over-expressing an apple gene for spermidine synthase (MdSPDS1), provided greater insight into the mechanisms of stress tolerance, leading to agronomic benefits, as well as being an elite line to be used to further elucidate the molecular mechanisms underlying stress tolerance that are exerted by spermidine (Spd). In this study, a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method, involving an annealing control primer (ACP)-based PCR (GeneFishing) system, was employed to identify genes that may be implicated in abiotic stress tolerance. A total of 42 transcripts that were differentially expressed between line #32 and wild-type (WT) Pear were identified. After cloning, 24 of these were found to exhibit significant sequence similarities with known genes from other species, and the remaining...
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spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic European Pear by exerting antioxidant activities
Transgenic Research, 2010Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:To verify whether spermidine synthase (SPDS) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic European Pear (Pyrus communis L. ‘Ballad’) line #32 overexpressing apple SPDS (MdSPDS1), as well as a wild type (WT) line, were subjected to stress using either CdCl2, PbCl2, ZnCl2, or a combination thereof. Based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better than that of WT. Although SPDS expression levels and spermidine (Spd) contents in line #32 were higher than those in WT, possibly due to transgene (MdSPDS1) expression, no obvious inductions of SPDS expression and increases in Spd-content were observed by long-term stress treatments in both lines. When the glutathione (GSH) content was compared with or without stress in each line, GSH was significantly depleted in line #32 with stress, but not as much as in WT. The activities of glutathione reductase and superoxide dismutase and the content of malondialdehyde, an indicator for lipid peroxidation, changed upon stress toward a more favorable status for survival in line #32 than in WT. These antioxidant parameters were positively related to Spd-content. The accumulation of heavy metals tended to be less in line #32 than in WT except for Zn stress, and the Ca content showed an opposite trend. These results suggest that Spd-levels are implicated in enhanced heavy metal tolerance, possibly by exerting an antioxidant activity as well as by the properties of Spd per se including metal chelator.
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Aluminum tolerance in a spermidine synthase-overexpressing transgenic European Pear is correlated with the enhanced level of spermidine via alleviating oxidative status
Environmental and Experimental Botany, 2009Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:Abstract Aluminum (Al) stress is a major cause of poor crop yields, particularly in those countries where acid soil predominate. To verify whether polyamine can confer Al tolerance, in vitro shoots of a transgenic European Pear ( Pyrus communis L. ‘Ballad’) line #32 overexpressing apple spermidine synthase ( MdSPDS1 ) and the wild type (WT) were subjected to long-term stress for 30 μM AlCl 3 . Based on net increment of shoot height (SHI) or fresh weight (FWI) and morphological changes upon the stress, the performance of line #32 was much better than that of WT. Although SPDS expression levels and spermidine (Spd) titers in line #32 were higher than those in WT, firmly due to the transgene ( MdSPDS1 ) expression, no further induction of SPDS expression was observed from the long-term Al stress trial in both lines. While, Spd titers were considerably increased in both lines after the stress. The activities of superoxide dismutase (SOD) or glutathione reductase (GR) and the accumulation of proline or malondialdehyde (MDA) altered upon this stress toward a more favorable status for survival in the transgenic line #32 than in WT. These antioxidant parameters were closely related to Spd titer. Concentrations of calcium (Ca) and some co-factor metals of SOD in line #32 were diversely higher than that in WT after the stress. These evidences indicate that Spd is implicated in elevating of Al stress tolerance of the transgenic line #32 chiefly via ameliorating oxidative status as well as by affecting mineral element balance.
Xiao-peng Wen - One of the best experts on this subject based on the ideXlab platform.
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Identification of differentially-expressed genes potentially related to stress tolerance in a transgenic line of European Pear over-expressing an apple spermidine synthase gene (MdSPDS1)
The Journal of Horticultural Science and Biotechnology, 2011Co-Authors: Xiao-peng Wen, Yusuke Ban, Xiaoming Pang, Takaya MoriguchiAbstract:SummaryPolyamines confer abiotic stress tolerance through mechanisms that are not yet fully understood. Previous studies on a transgenic line of European Pear (Pyrus communis L., line #32), over-expressing an apple gene for spermidine synthase (MdSPDS1), provided greater insight into the mechanisms of stress tolerance, leading to agronomic benefits, as well as being an elite line to be used to further elucidate the molecular mechanisms underlying stress tolerance that are exerted by spermidine (Spd). In this study, a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method, involving an annealing control primer (ACP)-based PCR (GeneFishing) system, was employed to identify genes that may be implicated in abiotic stress tolerance. A total of 42 transcripts that were differentially expressed between line #32 and wild-type (WT) Pear were identified. After cloning, 24 of these were found to exhibit significant sequence similarities with known genes from other species, and the remaining...
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spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic European Pear by exerting antioxidant activities
Transgenic Research, 2010Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:To verify whether spermidine synthase (SPDS) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic European Pear (Pyrus communis L. ‘Ballad’) line #32 overexpressing apple SPDS (MdSPDS1), as well as a wild type (WT) line, were subjected to stress using either CdCl2, PbCl2, ZnCl2, or a combination thereof. Based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better than that of WT. Although SPDS expression levels and spermidine (Spd) contents in line #32 were higher than those in WT, possibly due to transgene (MdSPDS1) expression, no obvious inductions of SPDS expression and increases in Spd-content were observed by long-term stress treatments in both lines. When the glutathione (GSH) content was compared with or without stress in each line, GSH was significantly depleted in line #32 with stress, but not as much as in WT. The activities of glutathione reductase and superoxide dismutase and the content of malondialdehyde, an indicator for lipid peroxidation, changed upon stress toward a more favorable status for survival in line #32 than in WT. These antioxidant parameters were positively related to Spd-content. The accumulation of heavy metals tended to be less in line #32 than in WT except for Zn stress, and the Ca content showed an opposite trend. These results suggest that Spd-levels are implicated in enhanced heavy metal tolerance, possibly by exerting an antioxidant activity as well as by the properties of Spd per se including metal chelator.
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Aluminum tolerance in a spermidine synthase-overexpressing transgenic European Pear is correlated with the enhanced level of spermidine via alleviating oxidative status
Environmental and Experimental Botany, 2009Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:Abstract Aluminum (Al) stress is a major cause of poor crop yields, particularly in those countries where acid soil predominate. To verify whether polyamine can confer Al tolerance, in vitro shoots of a transgenic European Pear ( Pyrus communis L. ‘Ballad’) line #32 overexpressing apple spermidine synthase ( MdSPDS1 ) and the wild type (WT) were subjected to long-term stress for 30 μM AlCl 3 . Based on net increment of shoot height (SHI) or fresh weight (FWI) and morphological changes upon the stress, the performance of line #32 was much better than that of WT. Although SPDS expression levels and spermidine (Spd) titers in line #32 were higher than those in WT, firmly due to the transgene ( MdSPDS1 ) expression, no further induction of SPDS expression was observed from the long-term Al stress trial in both lines. While, Spd titers were considerably increased in both lines after the stress. The activities of superoxide dismutase (SOD) or glutathione reductase (GR) and the accumulation of proline or malondialdehyde (MDA) altered upon this stress toward a more favorable status for survival in the transgenic line #32 than in WT. These antioxidant parameters were closely related to Spd titer. Concentrations of calcium (Ca) and some co-factor metals of SOD in line #32 were diversely higher than that in WT after the stress. These evidences indicate that Spd is implicated in elevating of Al stress tolerance of the transgenic line #32 chiefly via ameliorating oxidative status as well as by affecting mineral element balance.
Yusuke Ban - One of the best experts on this subject based on the ideXlab platform.
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Identification of differentially-expressed genes potentially related to stress tolerance in a transgenic line of European Pear over-expressing an apple spermidine synthase gene (MdSPDS1)
The Journal of Horticultural Science and Biotechnology, 2011Co-Authors: Xiao-peng Wen, Yusuke Ban, Xiaoming Pang, Takaya MoriguchiAbstract:SummaryPolyamines confer abiotic stress tolerance through mechanisms that are not yet fully understood. Previous studies on a transgenic line of European Pear (Pyrus communis L., line #32), over-expressing an apple gene for spermidine synthase (MdSPDS1), provided greater insight into the mechanisms of stress tolerance, leading to agronomic benefits, as well as being an elite line to be used to further elucidate the molecular mechanisms underlying stress tolerance that are exerted by spermidine (Spd). In this study, a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method, involving an annealing control primer (ACP)-based PCR (GeneFishing) system, was employed to identify genes that may be implicated in abiotic stress tolerance. A total of 42 transcripts that were differentially expressed between line #32 and wild-type (WT) Pear were identified. After cloning, 24 of these were found to exhibit significant sequence similarities with known genes from other species, and the remaining...
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spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic European Pear by exerting antioxidant activities
Transgenic Research, 2010Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:To verify whether spermidine synthase (SPDS) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic European Pear (Pyrus communis L. ‘Ballad’) line #32 overexpressing apple SPDS (MdSPDS1), as well as a wild type (WT) line, were subjected to stress using either CdCl2, PbCl2, ZnCl2, or a combination thereof. Based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better than that of WT. Although SPDS expression levels and spermidine (Spd) contents in line #32 were higher than those in WT, possibly due to transgene (MdSPDS1) expression, no obvious inductions of SPDS expression and increases in Spd-content were observed by long-term stress treatments in both lines. When the glutathione (GSH) content was compared with or without stress in each line, GSH was significantly depleted in line #32 with stress, but not as much as in WT. The activities of glutathione reductase and superoxide dismutase and the content of malondialdehyde, an indicator for lipid peroxidation, changed upon stress toward a more favorable status for survival in line #32 than in WT. These antioxidant parameters were positively related to Spd-content. The accumulation of heavy metals tended to be less in line #32 than in WT except for Zn stress, and the Ca content showed an opposite trend. These results suggest that Spd-levels are implicated in enhanced heavy metal tolerance, possibly by exerting an antioxidant activity as well as by the properties of Spd per se including metal chelator.
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Aluminum tolerance in a spermidine synthase-overexpressing transgenic European Pear is correlated with the enhanced level of spermidine via alleviating oxidative status
Environmental and Experimental Botany, 2009Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:Abstract Aluminum (Al) stress is a major cause of poor crop yields, particularly in those countries where acid soil predominate. To verify whether polyamine can confer Al tolerance, in vitro shoots of a transgenic European Pear ( Pyrus communis L. ‘Ballad’) line #32 overexpressing apple spermidine synthase ( MdSPDS1 ) and the wild type (WT) were subjected to long-term stress for 30 μM AlCl 3 . Based on net increment of shoot height (SHI) or fresh weight (FWI) and morphological changes upon the stress, the performance of line #32 was much better than that of WT. Although SPDS expression levels and spermidine (Spd) titers in line #32 were higher than those in WT, firmly due to the transgene ( MdSPDS1 ) expression, no further induction of SPDS expression was observed from the long-term Al stress trial in both lines. While, Spd titers were considerably increased in both lines after the stress. The activities of superoxide dismutase (SOD) or glutathione reductase (GR) and the accumulation of proline or malondialdehyde (MDA) altered upon this stress toward a more favorable status for survival in the transgenic line #32 than in WT. These antioxidant parameters were closely related to Spd titer. Concentrations of calcium (Ca) and some co-factor metals of SOD in line #32 were diversely higher than that in WT after the stress. These evidences indicate that Spd is implicated in elevating of Al stress tolerance of the transgenic line #32 chiefly via ameliorating oxidative status as well as by affecting mineral element balance.
Hiromichi Inoue - One of the best experts on this subject based on the ideXlab platform.
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spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic European Pear by exerting antioxidant activities
Transgenic Research, 2010Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:To verify whether spermidine synthase (SPDS) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic European Pear (Pyrus communis L. ‘Ballad’) line #32 overexpressing apple SPDS (MdSPDS1), as well as a wild type (WT) line, were subjected to stress using either CdCl2, PbCl2, ZnCl2, or a combination thereof. Based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better than that of WT. Although SPDS expression levels and spermidine (Spd) contents in line #32 were higher than those in WT, possibly due to transgene (MdSPDS1) expression, no obvious inductions of SPDS expression and increases in Spd-content were observed by long-term stress treatments in both lines. When the glutathione (GSH) content was compared with or without stress in each line, GSH was significantly depleted in line #32 with stress, but not as much as in WT. The activities of glutathione reductase and superoxide dismutase and the content of malondialdehyde, an indicator for lipid peroxidation, changed upon stress toward a more favorable status for survival in line #32 than in WT. These antioxidant parameters were positively related to Spd-content. The accumulation of heavy metals tended to be less in line #32 than in WT except for Zn stress, and the Ca content showed an opposite trend. These results suggest that Spd-levels are implicated in enhanced heavy metal tolerance, possibly by exerting an antioxidant activity as well as by the properties of Spd per se including metal chelator.
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Aluminum tolerance in a spermidine synthase-overexpressing transgenic European Pear is correlated with the enhanced level of spermidine via alleviating oxidative status
Environmental and Experimental Botany, 2009Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:Abstract Aluminum (Al) stress is a major cause of poor crop yields, particularly in those countries where acid soil predominate. To verify whether polyamine can confer Al tolerance, in vitro shoots of a transgenic European Pear ( Pyrus communis L. ‘Ballad’) line #32 overexpressing apple spermidine synthase ( MdSPDS1 ) and the wild type (WT) were subjected to long-term stress for 30 μM AlCl 3 . Based on net increment of shoot height (SHI) or fresh weight (FWI) and morphological changes upon the stress, the performance of line #32 was much better than that of WT. Although SPDS expression levels and spermidine (Spd) titers in line #32 were higher than those in WT, firmly due to the transgene ( MdSPDS1 ) expression, no further induction of SPDS expression was observed from the long-term Al stress trial in both lines. While, Spd titers were considerably increased in both lines after the stress. The activities of superoxide dismutase (SOD) or glutathione reductase (GR) and the accumulation of proline or malondialdehyde (MDA) altered upon this stress toward a more favorable status for survival in the transgenic line #32 than in WT. These antioxidant parameters were closely related to Spd titer. Concentrations of calcium (Ca) and some co-factor metals of SOD in line #32 were diversely higher than that in WT after the stress. These evidences indicate that Spd is implicated in elevating of Al stress tolerance of the transgenic line #32 chiefly via ameliorating oxidative status as well as by affecting mineral element balance.
Narumi Matsuda - One of the best experts on this subject based on the ideXlab platform.
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spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic European Pear by exerting antioxidant activities
Transgenic Research, 2010Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:To verify whether spermidine synthase (SPDS) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic European Pear (Pyrus communis L. ‘Ballad’) line #32 overexpressing apple SPDS (MdSPDS1), as well as a wild type (WT) line, were subjected to stress using either CdCl2, PbCl2, ZnCl2, or a combination thereof. Based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better than that of WT. Although SPDS expression levels and spermidine (Spd) contents in line #32 were higher than those in WT, possibly due to transgene (MdSPDS1) expression, no obvious inductions of SPDS expression and increases in Spd-content were observed by long-term stress treatments in both lines. When the glutathione (GSH) content was compared with or without stress in each line, GSH was significantly depleted in line #32 with stress, but not as much as in WT. The activities of glutathione reductase and superoxide dismutase and the content of malondialdehyde, an indicator for lipid peroxidation, changed upon stress toward a more favorable status for survival in line #32 than in WT. These antioxidant parameters were positively related to Spd-content. The accumulation of heavy metals tended to be less in line #32 than in WT except for Zn stress, and the Ca content showed an opposite trend. These results suggest that Spd-levels are implicated in enhanced heavy metal tolerance, possibly by exerting an antioxidant activity as well as by the properties of Spd per se including metal chelator.
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Aluminum tolerance in a spermidine synthase-overexpressing transgenic European Pear is correlated with the enhanced level of spermidine via alleviating oxidative status
Environmental and Experimental Botany, 2009Co-Authors: Xiao-peng Wen, Yusuke Ban, Hiromichi Inoue, Narumi Matsuda, Takaya MoriguchiAbstract:Abstract Aluminum (Al) stress is a major cause of poor crop yields, particularly in those countries where acid soil predominate. To verify whether polyamine can confer Al tolerance, in vitro shoots of a transgenic European Pear ( Pyrus communis L. ‘Ballad’) line #32 overexpressing apple spermidine synthase ( MdSPDS1 ) and the wild type (WT) were subjected to long-term stress for 30 μM AlCl 3 . Based on net increment of shoot height (SHI) or fresh weight (FWI) and morphological changes upon the stress, the performance of line #32 was much better than that of WT. Although SPDS expression levels and spermidine (Spd) titers in line #32 were higher than those in WT, firmly due to the transgene ( MdSPDS1 ) expression, no further induction of SPDS expression was observed from the long-term Al stress trial in both lines. While, Spd titers were considerably increased in both lines after the stress. The activities of superoxide dismutase (SOD) or glutathione reductase (GR) and the accumulation of proline or malondialdehyde (MDA) altered upon this stress toward a more favorable status for survival in the transgenic line #32 than in WT. These antioxidant parameters were closely related to Spd titer. Concentrations of calcium (Ca) and some co-factor metals of SOD in line #32 were diversely higher than that in WT after the stress. These evidences indicate that Spd is implicated in elevating of Al stress tolerance of the transgenic line #32 chiefly via ameliorating oxidative status as well as by affecting mineral element balance.
