The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform

Agapios Sachinidis - One of the best experts on this subject based on the ideXlab platform.

  • functional implications of cav2 3 r type voltage gated calcium channels in the murine auditory system novel vistas from brainstem Evoked Response Audiometry
    European Journal of Neuroscience, 2020
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Ca(v)1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Ca(v)2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Ca(v)2.3 in hearing physiology. Thus, we performed auditory profiling of Ca(v)2.3(+/+) controls, heterozygous Ca(v)2.3(+/-) mice and Ca(v)2.3 null mutants (Ca(v)2.3(-/-)) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Ca(v)2.3(+/-) mice from both genders, whereas no alterations were observed in Ca(v)2.3(-/-) mice. Similar observations were made for tone burst-related ABRs in both genders. However, Ca(v)2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave W-I and W-III amplitude in mutant animals. In addition, alterations in W-I-W-IV interwave interval were observed in female Ca(v)2.3(+/-) mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Ca(v)2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Functional implications of Cav2.3 R‐type voltage‐gated calcium channels in the murine auditory system – novel vistas from brainstem‐Evoked Response Audiometry
    The European journal of neuroscience, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Cav 1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Cav 2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Cav 2.3 in hearing physiology. Thus, we performed auditory profiling of Cav 2.3+/+ controls, heterozygous Cav 2.3+/- mice and Cav 2.3 null mutants (Cav 2.3-/- ) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Cav 2.3+/- mice from both genders, whereas no alterations were observed in Cav 2.3-/- mice. Similar observations were made for tone burst-related ABRs in both genders. However, Cav 2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave WI and WIII amplitude in mutant animals. In addition, alterations in WI -WIV interwave interval were observed in female Cav 2.3+/- mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Cav 2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice.
    Journal of visualized experiments : JoVE, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Agapios Sachinidis, Karl Broich, Carola Wormuth
    Abstract:

    Brainstem Evoked Response Audiometry (BERA) is of central relevance in the clinical neurophysiology. As other Evoked potential (EP) techniques, such as visually Evoked potentials (VEPs) or somatosensory Evoked potentials (SEPs), the auditory Evoked potentials (AEPs) are triggered by the repetitive presentation of identical stimuli, the electroencephalographic (EEG) Response of which is subsequently averaged resulting in distinct positive (p) and negative (n) deflections. In humans, both the amplitude and the latency of individual peaks can be used to characterize alterations in synchronization and conduction velocity in the underlying neuronal circuitries. Importantly, AEPs are also applied in basic and preclinical science to identify and characterize the auditory function in pharmacological and genetic animal models. Even more, animal models in combination with pharmacological testing are utilized to investigate for potential benefits in the treatment of sensorineural hearing loss (e.g., age- or noise-induced hearing deficits). Here we provide a detailed and integrative description of how to record auditory brainstem-Evoked Responses (ABRs) in mice using click and tone-burst application. A specific focus of this protocol is on pre-experimental animal housing, anesthesia, ABR recording, ABR filtering processes, automated wavelet-based amplitude growth function analysis, and latency detection.

Andreas Lundt - One of the best experts on this subject based on the ideXlab platform.

  • functional implications of cav2 3 r type voltage gated calcium channels in the murine auditory system novel vistas from brainstem Evoked Response Audiometry
    European Journal of Neuroscience, 2020
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Ca(v)1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Ca(v)2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Ca(v)2.3 in hearing physiology. Thus, we performed auditory profiling of Ca(v)2.3(+/+) controls, heterozygous Ca(v)2.3(+/-) mice and Ca(v)2.3 null mutants (Ca(v)2.3(-/-)) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Ca(v)2.3(+/-) mice from both genders, whereas no alterations were observed in Ca(v)2.3(-/-) mice. Similar observations were made for tone burst-related ABRs in both genders. However, Ca(v)2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave W-I and W-III amplitude in mutant animals. In addition, alterations in W-I-W-IV interwave interval were observed in female Ca(v)2.3(+/-) mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Ca(v)2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Functional implications of Cav2.3 R‐type voltage‐gated calcium channels in the murine auditory system – novel vistas from brainstem‐Evoked Response Audiometry
    The European journal of neuroscience, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Cav 1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Cav 2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Cav 2.3 in hearing physiology. Thus, we performed auditory profiling of Cav 2.3+/+ controls, heterozygous Cav 2.3+/- mice and Cav 2.3 null mutants (Cav 2.3-/- ) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Cav 2.3+/- mice from both genders, whereas no alterations were observed in Cav 2.3-/- mice. Similar observations were made for tone burst-related ABRs in both genders. However, Cav 2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave WI and WIII amplitude in mutant animals. In addition, alterations in WI -WIV interwave interval were observed in female Cav 2.3+/- mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Cav 2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice.
    Journal of visualized experiments : JoVE, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Agapios Sachinidis, Karl Broich, Carola Wormuth
    Abstract:

    Brainstem Evoked Response Audiometry (BERA) is of central relevance in the clinical neurophysiology. As other Evoked potential (EP) techniques, such as visually Evoked potentials (VEPs) or somatosensory Evoked potentials (SEPs), the auditory Evoked potentials (AEPs) are triggered by the repetitive presentation of identical stimuli, the electroencephalographic (EEG) Response of which is subsequently averaged resulting in distinct positive (p) and negative (n) deflections. In humans, both the amplitude and the latency of individual peaks can be used to characterize alterations in synchronization and conduction velocity in the underlying neuronal circuitries. Importantly, AEPs are also applied in basic and preclinical science to identify and characterize the auditory function in pharmacological and genetic animal models. Even more, animal models in combination with pharmacological testing are utilized to investigate for potential benefits in the treatment of sensorineural hearing loss (e.g., age- or noise-induced hearing deficits). Here we provide a detailed and integrative description of how to record auditory brainstem-Evoked Responses (ABRs) in mice using click and tone-burst application. A specific focus of this protocol is on pre-experimental animal housing, anesthesia, ABR recording, ABR filtering processes, automated wavelet-based amplitude growth function analysis, and latency detection.

Varun Raj Ginde - One of the best experts on this subject based on the ideXlab platform.

  • functional implications of cav2 3 r type voltage gated calcium channels in the murine auditory system novel vistas from brainstem Evoked Response Audiometry
    European Journal of Neuroscience, 2020
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Ca(v)1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Ca(v)2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Ca(v)2.3 in hearing physiology. Thus, we performed auditory profiling of Ca(v)2.3(+/+) controls, heterozygous Ca(v)2.3(+/-) mice and Ca(v)2.3 null mutants (Ca(v)2.3(-/-)) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Ca(v)2.3(+/-) mice from both genders, whereas no alterations were observed in Ca(v)2.3(-/-) mice. Similar observations were made for tone burst-related ABRs in both genders. However, Ca(v)2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave W-I and W-III amplitude in mutant animals. In addition, alterations in W-I-W-IV interwave interval were observed in female Ca(v)2.3(+/-) mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Ca(v)2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Functional implications of Cav2.3 R‐type voltage‐gated calcium channels in the murine auditory system – novel vistas from brainstem‐Evoked Response Audiometry
    The European journal of neuroscience, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Cav 1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Cav 2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Cav 2.3 in hearing physiology. Thus, we performed auditory profiling of Cav 2.3+/+ controls, heterozygous Cav 2.3+/- mice and Cav 2.3 null mutants (Cav 2.3-/- ) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Cav 2.3+/- mice from both genders, whereas no alterations were observed in Cav 2.3-/- mice. Similar observations were made for tone burst-related ABRs in both genders. However, Cav 2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave WI and WIII amplitude in mutant animals. In addition, alterations in WI -WIV interwave interval were observed in female Cav 2.3+/- mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Cav 2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

Robin Seidel - One of the best experts on this subject based on the ideXlab platform.

  • functional implications of cav2 3 r type voltage gated calcium channels in the murine auditory system novel vistas from brainstem Evoked Response Audiometry
    European Journal of Neuroscience, 2020
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Ca(v)1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Ca(v)2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Ca(v)2.3 in hearing physiology. Thus, we performed auditory profiling of Ca(v)2.3(+/+) controls, heterozygous Ca(v)2.3(+/-) mice and Ca(v)2.3 null mutants (Ca(v)2.3(-/-)) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Ca(v)2.3(+/-) mice from both genders, whereas no alterations were observed in Ca(v)2.3(-/-) mice. Similar observations were made for tone burst-related ABRs in both genders. However, Ca(v)2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave W-I and W-III amplitude in mutant animals. In addition, alterations in W-I-W-IV interwave interval were observed in female Ca(v)2.3(+/-) mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Ca(v)2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Functional implications of Cav2.3 R‐type voltage‐gated calcium channels in the murine auditory system – novel vistas from brainstem‐Evoked Response Audiometry
    The European journal of neuroscience, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Cav 1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Cav 2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Cav 2.3 in hearing physiology. Thus, we performed auditory profiling of Cav 2.3+/+ controls, heterozygous Cav 2.3+/- mice and Cav 2.3 null mutants (Cav 2.3-/- ) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Cav 2.3+/- mice from both genders, whereas no alterations were observed in Cav 2.3-/- mice. Similar observations were made for tone burst-related ABRs in both genders. However, Cav 2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave WI and WIII amplitude in mutant animals. In addition, alterations in WI -WIV interwave interval were observed in female Cav 2.3+/- mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Cav 2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

Julien Soos - One of the best experts on this subject based on the ideXlab platform.

  • functional implications of cav2 3 r type voltage gated calcium channels in the murine auditory system novel vistas from brainstem Evoked Response Audiometry
    European Journal of Neuroscience, 2020
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Ca(v)1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Ca(v)2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Ca(v)2.3 in hearing physiology. Thus, we performed auditory profiling of Ca(v)2.3(+/+) controls, heterozygous Ca(v)2.3(+/-) mice and Ca(v)2.3 null mutants (Ca(v)2.3(-/-)) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Ca(v)2.3(+/-) mice from both genders, whereas no alterations were observed in Ca(v)2.3(-/-) mice. Similar observations were made for tone burst-related ABRs in both genders. However, Ca(v)2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave W-I and W-III amplitude in mutant animals. In addition, alterations in W-I-W-IV interwave interval were observed in female Ca(v)2.3(+/-) mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Ca(v)2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Functional implications of Cav2.3 R‐type voltage‐gated calcium channels in the murine auditory system – novel vistas from brainstem‐Evoked Response Audiometry
    The European journal of neuroscience, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Robin Seidel, Varun Raj Ginde, Agapios Sachinidis
    Abstract:

    Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Cav 1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Cav 2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Cav 2.3 in hearing physiology. Thus, we performed auditory profiling of Cav 2.3+/+ controls, heterozygous Cav 2.3+/- mice and Cav 2.3 null mutants (Cav 2.3-/- ) using brainstem-Evoked Response Audiometry. Interestingly, click-Evoked auditory brainstem Responses (ABRs) revealed increased hearing thresholds in Cav 2.3+/- mice from both genders, whereas no alterations were observed in Cav 2.3-/- mice. Similar observations were made for tone burst-related ABRs in both genders. However, Cav 2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave WI and WIII amplitude in mutant animals. In addition, alterations in WI -WIV interwave interval were observed in female Cav 2.3+/- mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Cav 2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

  • Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice.
    Journal of visualized experiments : JoVE, 2019
    Co-Authors: Andreas Lundt, Julien Soos, Christina Henseler, Muhammad Imran Arshaad, Ralf Müller, Dan Ehninger, Jürgen Hescheler, Agapios Sachinidis, Karl Broich, Carola Wormuth
    Abstract:

    Brainstem Evoked Response Audiometry (BERA) is of central relevance in the clinical neurophysiology. As other Evoked potential (EP) techniques, such as visually Evoked potentials (VEPs) or somatosensory Evoked potentials (SEPs), the auditory Evoked potentials (AEPs) are triggered by the repetitive presentation of identical stimuli, the electroencephalographic (EEG) Response of which is subsequently averaged resulting in distinct positive (p) and negative (n) deflections. In humans, both the amplitude and the latency of individual peaks can be used to characterize alterations in synchronization and conduction velocity in the underlying neuronal circuitries. Importantly, AEPs are also applied in basic and preclinical science to identify and characterize the auditory function in pharmacological and genetic animal models. Even more, animal models in combination with pharmacological testing are utilized to investigate for potential benefits in the treatment of sensorineural hearing loss (e.g., age- or noise-induced hearing deficits). Here we provide a detailed and integrative description of how to record auditory brainstem-Evoked Responses (ABRs) in mice using click and tone-burst application. A specific focus of this protocol is on pre-experimental animal housing, anesthesia, ABR recording, ABR filtering processes, automated wavelet-based amplitude growth function analysis, and latency detection.