The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform

Jianbo Liu - One of the best experts on this subject based on the ideXlab platform.

  • Competitive host-guest interaction between β-cyclodextrin polymer and pyrene-labeled probes for fluorescence analyses.
    Analytical chemistry, 2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily ho...

  • Competitive Host–Guest Interaction between β‑Cyclodextrin Polymer and Pyrene-Labeled Probes for Fluorescence Analyses
    2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily host–guest interact with polyβ CD and emit stronger fluorescence than pyrene-labeled probe. In addition, the successful detection of adenosine is also demonstrated by using the similar sensing scheme. Although this scheme might be easily interfered by some biomolecules in the real test sample, it holds promising potential for detecting a broad range of other types of aptamer-binding chemicals and biomolecules

Pei Liu - One of the best experts on this subject based on the ideXlab platform.

  • Competitive host-guest interaction between β-cyclodextrin polymer and pyrene-labeled probes for fluorescence analyses.
    Analytical chemistry, 2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily ho...

  • Competitive Host–Guest Interaction between β‑Cyclodextrin Polymer and Pyrene-Labeled Probes for Fluorescence Analyses
    2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily host–guest interact with polyβ CD and emit stronger fluorescence than pyrene-labeled probe. In addition, the successful detection of adenosine is also demonstrated by using the similar sensing scheme. Although this scheme might be easily interfered by some biomolecules in the real test sample, it holds promising potential for detecting a broad range of other types of aptamer-binding chemicals and biomolecules

Jin Huang - One of the best experts on this subject based on the ideXlab platform.

  • Competitive host-guest interaction between β-cyclodextrin polymer and pyrene-labeled probes for fluorescence analyses.
    Analytical chemistry, 2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily ho...

  • Competitive Host–Guest Interaction between β‑Cyclodextrin Polymer and Pyrene-Labeled Probes for Fluorescence Analyses
    2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily host–guest interact with polyβ CD and emit stronger fluorescence than pyrene-labeled probe. In addition, the successful detection of adenosine is also demonstrated by using the similar sensing scheme. Although this scheme might be easily interfered by some biomolecules in the real test sample, it holds promising potential for detecting a broad range of other types of aptamer-binding chemicals and biomolecules

Qing Wang - One of the best experts on this subject based on the ideXlab platform.

  • Competitive host-guest interaction between β-cyclodextrin polymer and pyrene-labeled probes for fluorescence analyses.
    Analytical chemistry, 2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily ho...

  • Competitive Host–Guest Interaction between β‑Cyclodextrin Polymer and Pyrene-Labeled Probes for Fluorescence Analyses
    2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily host–guest interact with polyβ CD and emit stronger fluorescence than pyrene-labeled probe. In addition, the successful detection of adenosine is also demonstrated by using the similar sensing scheme. Although this scheme might be easily interfered by some biomolecules in the real test sample, it holds promising potential for detecting a broad range of other types of aptamer-binding chemicals and biomolecules

Kemin Wang - One of the best experts on this subject based on the ideXlab platform.

  • Competitive host-guest interaction between β-cyclodextrin polymer and pyrene-labeled probes for fluorescence analyses.
    Analytical chemistry, 2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily ho...

  • Competitive Host–Guest Interaction between β‑Cyclodextrin Polymer and Pyrene-Labeled Probes for Fluorescence Analyses
    2015
    Co-Authors: Pei Liu, Shan Sun, Xiaochen Guo, Xiaohai Yang, Jin Huang, Kemin Wang, Qing Wang, Jianbo Liu
    Abstract:

    We developed a novel homogeneous fluorescence analysis based on a novel competitive host–guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily host–guest interact with polyβ CD and emit stronger fluorescence than pyrene-labeled probe. In addition, the successful detection of adenosine is also demonstrated by using the similar sensing scheme. Although this scheme might be easily interfered by some biomolecules in the real test sample, it holds promising potential for detecting a broad range of other types of aptamer-binding chemicals and biomolecules