The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform

Liming Wang - One of the best experts on this subject based on the ideXlab platform.

  • Chondrogenic Differentiation Could Be Induced by Autologous Bone Marrow Mesenchymal Stem Cell–Derived Extracellular Matrix Scaffolds Without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E+ or E− group). Atelocollagen scaffolds were used as controls (C+ or C− group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E+, E−, and C+ grou...

  • chondrogenic differentiation could be induced by autologous bone marrow mesenchymal stem cell derived extracellular matrix scaffolds without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E+ or E− group). Atelocollagen scaffolds were used as controls (C+ or C− group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E+, E−, and C+ grou...

  • Chondrogenic Differentiation Could Be Induced by Autologous Bone Marrow Mesenchymal Stem Cell–Derived Extracellular Matrix Scaffolds Without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Chengzhe Jin, Bo Wei, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E(+) or E(-) group). Atelocollagen scaffolds were used as controls (C(+) or C(-) group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E(+), E(-), and C(+) groups. In addition, BMSCs in each scaffold (E group or C group) were preconditioned with chondrogenic media in vitro for 1 week, and then implanted in the backs of nude mice for 3 weeks. Three weeks later, cartilage matrix formation (proteoglycan and type II collagen) was achieved only in the E group, confirmed by safranin O staining and immunohistochemical staining for type II collagen. Taken together, these results indicate that aBMSC-dECM scaffolds could induce chondrogenic differentiation. Thus, they could be successful candidate scaffolds for cartilage tissue engineering.

Cheng Tang - One of the best experts on this subject based on the ideXlab platform.

  • Chondrogenic Differentiation Could Be Induced by Autologous Bone Marrow Mesenchymal Stem Cell–Derived Extracellular Matrix Scaffolds Without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E+ or E− group). Atelocollagen scaffolds were used as controls (C+ or C− group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E+, E−, and C+ grou...

  • chondrogenic differentiation could be induced by autologous bone marrow mesenchymal stem cell derived extracellular matrix scaffolds without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E+ or E− group). Atelocollagen scaffolds were used as controls (C+ or C− group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E+, E−, and C+ grou...

  • Chondrogenic Differentiation Could Be Induced by Autologous Bone Marrow Mesenchymal Stem Cell–Derived Extracellular Matrix Scaffolds Without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Chengzhe Jin, Bo Wei, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E(+) or E(-) group). Atelocollagen scaffolds were used as controls (C(+) or C(-) group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E(+), E(-), and C(+) groups. In addition, BMSCs in each scaffold (E group or C group) were preconditioned with chondrogenic media in vitro for 1 week, and then implanted in the backs of nude mice for 3 weeks. Three weeks later, cartilage matrix formation (proteoglycan and type II collagen) was achieved only in the E group, confirmed by safranin O staining and immunohistochemical staining for type II collagen. Taken together, these results indicate that aBMSC-dECM scaffolds could induce chondrogenic differentiation. Thus, they could be successful candidate scaffolds for cartilage tissue engineering.

Jin Bo Tang - One of the best experts on this subject based on the ideXlab platform.

  • 684. Viral Gene Therapy in Tendon Healing: Adeno-Associated Virus-2 but Not Other Serotypes Effectively Transduces Intrasynovial Tenocytes with Persistent Expression of the Transgene
    Molecular Therapy, 2005
    Co-Authors: Xiao Tian Wang, Jin Bo Tang
    Abstract:

    Purpose: Transfer of Exogenous Growth Factor genes to injured tendons is a new and promising method for strengthening tendon repairs. Genes have been delivered to tenocytes through plasmid or adenoviral vectors, but gene transfer through adeno-associated viral (AAV) vectors has not been reported. It is not known whether AAVs can effectively transduce tenocytes and whether transduction rate is different for different AAV serotypes. We explored the efficiency of transduction of intrasynovial tenocytes with 7 serotypes of AAV and the persistency of its expression of a Growth Factor transgene.

  • tendon healing in vitro modification of tenocytes with Exogenous vascular endothelial Growth Factor gene increases expression of transforming Growth Factor β but minimally affects expression of collagen genes
    Journal of Hand Surgery (European Volume), 2005
    Co-Authors: Xiao Tian Wang, Jin Bo Tang
    Abstract:

    Purpose It is not clear how the transfer of Exogenous Growth Factor genes to tenocytes affects collagen production. An increase in collagen production enhances the repair but an increase in Growth Factors that stimulate tissue fibrosis may cause adhesion. Gene therapy is a new way to regulate tendon healing but it has been explored rarely. We genetically modified tenocytes with the vascular endothelial Growth Factor (VEGF) gene and investigated the expression of the genes for collagen production in an in vitro model of the proliferating tenocytes. Methods Tenocytes were obtained from cultures of rat intrasynovial tendons and distributed randomly to 25 dishes. The tenocytes in the experimental dishes (n = 9) were treated for 12 hours with plasmid containing the VEGF complementary deoxyribonucleic acid and then were cultured for 5 days; the tenocytes in the control dishes (n = 8) did not receive the Exogenous gene. Tenocytes in the other dishes received Exogenous platelet-derived Growth Factor (PDGF) gene for comparison of the effects of VEGF gene therapy. Efficiency of the gene transfer was evaluated by presence of the transgene in the tenocytes which was detected by reverse transcription polymerase chain reactions. Levels of expression of types I and III collagen and transforming Growth Factor (TGF)-β genes were determined by quantitative analysis of the products of reverse transcription polymerase chain reactions. Results Expression of the TGF-β gene increased significantly in the cells treated with Exogenous VEGF cDNA. Expression of type I and III collagen genes by tenocytes was affected minimally by transfer of the VEGF gene to the tenocytes and was significantly weaker than that stimulated by PDGF gene therapy. Efficient gene transfer was confirmed by the presence of the VEGF complementary deoxyribonucleic acid in the tenocytes receiving the transferred gene. Conclusions Transfer of Exogenous VEGF gene has very limited effects on the promotion of collagen production in the proliferating tenocytes. This study suggests that VEGF gene therapy is not as beneficial as PDGF gene therapy to tendon healing and may increase the activities of TGF-β that are associated with adhesion formations.

Yan Xu - One of the best experts on this subject based on the ideXlab platform.

  • Chondrogenic Differentiation Could Be Induced by Autologous Bone Marrow Mesenchymal Stem Cell–Derived Extracellular Matrix Scaffolds Without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E+ or E− group). Atelocollagen scaffolds were used as controls (C+ or C− group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E+, E−, and C+ grou...

  • chondrogenic differentiation could be induced by autologous bone marrow mesenchymal stem cell derived extracellular matrix scaffolds without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E+ or E− group). Atelocollagen scaffolds were used as controls (C+ or C− group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E+, E−, and C+ grou...

  • Chondrogenic Differentiation Could Be Induced by Autologous Bone Marrow Mesenchymal Stem Cell–Derived Extracellular Matrix Scaffolds Without Exogenous Growth Factor
    Tissue Engineering Part A, 2016
    Co-Authors: Cheng Tang, Chengzhe Jin, Bo Wei, Yan Xu, Liming Wang
    Abstract:

    We previously found that the combination of an autologous bone mesenchymal stem cell-derived extracellular matrix (aBMSC-dECM) scaffold with bone marrow stimulation could enhance hyaline cartilage regeneration. We suspected that chondrogenic differentiation could be induced by the aBMSC-dECM scaffold. This study aimed to investigate whether aBMSC-dECM scaffolds could promote chondrogenic differentiation without Exogenous Growth Factors. BMSCs were seeded on aBMSC-dECM scaffolds and cultured in vitro with or without transforming Growth Factor-β3 (E(+) or E(-) group). Atelocollagen scaffolds were used as controls (C(+) or C(-) group). The chondrogenic differentiation was evaluated by histological, biochemical, and real-time polymerase chain reaction assays. After 3 weeks, cartilage-like tissue with a homogeneous structure, a high cartilaginous matrix content (proteoglycan and type II collagen), and high expression levels of cartilage-associated genes (COL2A1, ACAN, and SOX9) were observed in the E(+), E(-), and C(+) groups. In addition, BMSCs in each scaffold (E group or C group) were preconditioned with chondrogenic media in vitro for 1 week, and then implanted in the backs of nude mice for 3 weeks. Three weeks later, cartilage matrix formation (proteoglycan and type II collagen) was achieved only in the E group, confirmed by safranin O staining and immunohistochemical staining for type II collagen. Taken together, these results indicate that aBMSC-dECM scaffolds could induce chondrogenic differentiation. Thus, they could be successful candidate scaffolds for cartilage tissue engineering.

Xiao Tian Wang - One of the best experts on this subject based on the ideXlab platform.

  • 684. Viral Gene Therapy in Tendon Healing: Adeno-Associated Virus-2 but Not Other Serotypes Effectively Transduces Intrasynovial Tenocytes with Persistent Expression of the Transgene
    Molecular Therapy, 2005
    Co-Authors: Xiao Tian Wang, Jin Bo Tang
    Abstract:

    Purpose: Transfer of Exogenous Growth Factor genes to injured tendons is a new and promising method for strengthening tendon repairs. Genes have been delivered to tenocytes through plasmid or adenoviral vectors, but gene transfer through adeno-associated viral (AAV) vectors has not been reported. It is not known whether AAVs can effectively transduce tenocytes and whether transduction rate is different for different AAV serotypes. We explored the efficiency of transduction of intrasynovial tenocytes with 7 serotypes of AAV and the persistency of its expression of a Growth Factor transgene.

  • tendon healing in vitro modification of tenocytes with Exogenous vascular endothelial Growth Factor gene increases expression of transforming Growth Factor β but minimally affects expression of collagen genes
    Journal of Hand Surgery (European Volume), 2005
    Co-Authors: Xiao Tian Wang, Jin Bo Tang
    Abstract:

    Purpose It is not clear how the transfer of Exogenous Growth Factor genes to tenocytes affects collagen production. An increase in collagen production enhances the repair but an increase in Growth Factors that stimulate tissue fibrosis may cause adhesion. Gene therapy is a new way to regulate tendon healing but it has been explored rarely. We genetically modified tenocytes with the vascular endothelial Growth Factor (VEGF) gene and investigated the expression of the genes for collagen production in an in vitro model of the proliferating tenocytes. Methods Tenocytes were obtained from cultures of rat intrasynovial tendons and distributed randomly to 25 dishes. The tenocytes in the experimental dishes (n = 9) were treated for 12 hours with plasmid containing the VEGF complementary deoxyribonucleic acid and then were cultured for 5 days; the tenocytes in the control dishes (n = 8) did not receive the Exogenous gene. Tenocytes in the other dishes received Exogenous platelet-derived Growth Factor (PDGF) gene for comparison of the effects of VEGF gene therapy. Efficiency of the gene transfer was evaluated by presence of the transgene in the tenocytes which was detected by reverse transcription polymerase chain reactions. Levels of expression of types I and III collagen and transforming Growth Factor (TGF)-β genes were determined by quantitative analysis of the products of reverse transcription polymerase chain reactions. Results Expression of the TGF-β gene increased significantly in the cells treated with Exogenous VEGF cDNA. Expression of type I and III collagen genes by tenocytes was affected minimally by transfer of the VEGF gene to the tenocytes and was significantly weaker than that stimulated by PDGF gene therapy. Efficient gene transfer was confirmed by the presence of the VEGF complementary deoxyribonucleic acid in the tenocytes receiving the transferred gene. Conclusions Transfer of Exogenous VEGF gene has very limited effects on the promotion of collagen production in the proliferating tenocytes. This study suggests that VEGF gene therapy is not as beneficial as PDGF gene therapy to tendon healing and may increase the activities of TGF-β that are associated with adhesion formations.