The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
Claire Boisset - One of the best experts on this subject based on the ideXlab platform.
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structural data on a bacterial exopolysaccharide produced by a deep sea alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Le T Costaouec, Jacqueline Ratiskol, Corinne Sinquin, Sylvia Colliecjouault, David Ropartz, Stephane Cerantola, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. Highlights ► Structural data of a complex bacterial exopolysaccharide of deep-sea origin are presented. ► This exopolysaccharide, named deepsane, is commercialised in cosmetics. ► Structural similarities with other exopolysaccharide-producing marine Alteromonas strains are reported.
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Structural data on a bacterial exopolysaccharide produced by a deep-sea Alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Stephane Cerantola, Jacqueline Ratiskol, Corinne Sinquin, David Ropartz, S. Colliec-jouault, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. (C) 2012 Elsevier Ltd. All rights reserved.
Yasuyuki Kawaharada - One of the best experts on this subject based on the ideXlab platform.
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differential regulation of the epr3 receptor coordinates membrane restricted rhizobial colonization of root nodule primordia
Nature Communications, 2017Co-Authors: Yasuyuki Kawaharada, Mette Wibroe Nielsen, Simon Kelly, Euan K James, Kasper R Andersen, Sheena R Rasmussen, Winnie Fuchtbauer, Lene H Madsen, Anne B HeckmannAbstract:In Lotus japonicus, a LysM receptor kinase, EPR3, distinguishes compatible and incompatible rhizobial Exopolysaccharides at the epidermis. However, the role of this recognition system in bacterial colonization of the root interior is unknown. Here we show that EPR3 advances the intracellular infection mechanism that mediates infection thread invasion of the root cortex and nodule primordia. At the cellular level, Epr3 expression delineates progression of infection threads into nodule primordia and cortical infection thread formation is impaired in epr3 mutants. Genetic dissection of this developmental coordination showed that Epr3 is integrated into the symbiosis signal transduction pathways. Further analysis showed differential expression of Epr3 in the epidermis and cortical primordia and identified key transcription factors controlling this tissue specificity. These results suggest that exopolysaccharide recognition is reiterated during the progressing infection and that EPR3 perception of compatible exopolysaccharide promotes an intracellular cortical infection mechanism maintaining bacteria enclosed in plant membranes. TheLotus japonicus LysM receptor kinase EPR3 perceives rhizobial Exopolysaccharides to initiate infection of the root epidermis. Here the authors show that EPR3 also mediates infection thread progression in the root cortex and show that key transcription factors that regulate symbiosis specify the expression of Epr3.
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differential regulation of the epr3 receptor coordinates membrane restricted rhizobial colonization of root nodule primordia
Nature Communications, 2017Co-Authors: Yasuyuki Kawaharada, Mette Wibroe Nielsen, Simon Kelly, Euan K James, Kasper R Andersen, Sheena R Rasmussen, Winnie Fuchtbauer, Anne B Heckmann, Lene H Madsen, Simona RadutoiuAbstract:In Lotus japonicus, a LysM receptor kinase, EPR3, distinguishes compatible and incompatible rhizobial Exopolysaccharides at the epidermis. However, the role of this recognition system in bacterial colonization of the root interior is unknown. Here we show that EPR3 advances the intracellular infection mechanism that mediates infection thread invasion of the root cortex and nodule primordia. At the cellular level, Epr3 expression delineates progression of infection threads into nodule primordia and cortical infection thread formation is impaired in epr3 mutants. Genetic dissection of this developmental coordination showed that Epr3 is integrated into the symbiosis signal transduction pathways. Further analysis showed differential expression of Epr3 in the epidermis and cortical primordia and identified key transcription factors controlling this tissue specificity. These results suggest that exopolysaccharide recognition is reiterated during the progressing infection and that EPR3 perception of compatible exopolysaccharide promotes an intracellular cortical infection mechanism maintaining bacteria enclosed in plant membranes.
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receptor mediated exopolysaccharide perception controls bacterial infection
Nature, 2015Co-Authors: Yasuyuki Kawaharada, Simon Kelly, Wibroe M Nielsen, Christian T Hjuler, Kira Gysel, Artur Muszynski, Russell W Carlson, Mikkel B Thygesen, Niels Sandal, M H AsmussenAbstract:Rhizobium bacteria infect the roots of legumes, where they induce the formation of nitrogen-fixing root nodules. This symbiotic relationship is of agricultural importance as it reduces the need for nitrogen fertilizers. But how do legumes recognize these beneficial partners among thousands of incompatible soil bacteria they encounter? It is known that Exopolysaccharides on the surface of bacteria are important for interactions of these microorganisms with multicellular organisms and here Jens Stougaard and coworkers identify an exopolysaccharide receptor (EPR3) that mediates recognition of rhizobia in the wild legume Lotus japonicus. EPR3 expression is induced upon perception of bacterial signalling molecules known as Nod factors. The receptor recognizes compatible Exopolysaccharides, thus controlling the symbiotic infection.
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Receptor-mediated exopolysaccharide perception controls bacterial infection
Nature, 2015Co-Authors: Yasuyuki Kawaharada, Christian T Hjuler, Kira Gysel, Artur Muszynski, Russell W Carlson, Mikkel B Thygesen, Niels Sandal, S. Kelly, M. Wibroe Nielsen, M H AsmussenAbstract:Surface polysaccharides are important for bacterial interactions with multicellular organisms, and some are virulence factors in pathogens. In the legume–rhizobium symbiosis, bacterial Exopolysaccharides (EPS) are essential for the development of infected root nodules. We have identified a gene in Lotus japonicus , Epr3 , encoding a receptor-like kinase that controls this infection. We show that epr3 mutants are defective in perception of purified EPS, and that EPR3 binds EPS directly and distinguishes compatible and incompatible EPS in bacterial competition studies. Expression of Epr3 in epidermal cells within the susceptible root zone shows that the protein is involved in bacterial entry, while rhizobial and plant mutant studies suggest that Epr3 regulates bacterial passage through the plant’s epidermal cell layer. Finally, we show that Epr3 expression is inducible and dependent on host perception of bacterial nodulation (Nod) factors. Plant–bacterial compatibility and bacterial access to legume roots is thus regulated by a two-stage mechanism involving sequential receptor-mediated recognition of Nod factor and EPS signals. This paper describes the discovery of the exopolysaccharide receptor ( Epr3 ) in plants, and shows that its expression is induced upon perception of the bacterial Nod factors; the EPR3 receptor recognizes Exopolysaccharides on the surface of rhizobia, thus controlling the symbiotic infection of the roots of legumes. Rhizobium bacteria infect the roots of legumes, where they induce the formation of nitrogen-fixing root nodules. This symbiotic relationship is of agricultural importance as it reduces the need for nitrogen fertilizers. But how do legumes recognize these beneficial partners among thousands of incompatible soil bacteria they encounter? It is known that Exopolysaccharides on the surface of bacteria are important for interactions of these microorganisms with multicellular organisms and here Jens Stougaard and coworkers identify an exopolysaccharide receptor (EPR3) that mediates recognition of rhizobia in the wild legume Lotus japonicus . EPR3 expression is induced upon perception of bacterial signalling molecules known as Nod factors. The receptor recognizes compatible Exopolysaccharides, thus controlling the symbiotic infection.
Stephane Cerantola - One of the best experts on this subject based on the ideXlab platform.
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structural data on a bacterial exopolysaccharide produced by a deep sea alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Le T Costaouec, Jacqueline Ratiskol, Corinne Sinquin, Sylvia Colliecjouault, David Ropartz, Stephane Cerantola, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. Highlights ► Structural data of a complex bacterial exopolysaccharide of deep-sea origin are presented. ► This exopolysaccharide, named deepsane, is commercialised in cosmetics. ► Structural similarities with other exopolysaccharide-producing marine Alteromonas strains are reported.
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Structural data on a bacterial exopolysaccharide produced by a deep-sea Alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Stephane Cerantola, Jacqueline Ratiskol, Corinne Sinquin, David Ropartz, S. Colliec-jouault, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. (C) 2012 Elsevier Ltd. All rights reserved.
Jacqueline Ratiskol - One of the best experts on this subject based on the ideXlab platform.
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structural data on a bacterial exopolysaccharide produced by a deep sea alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Le T Costaouec, Jacqueline Ratiskol, Corinne Sinquin, Sylvia Colliecjouault, David Ropartz, Stephane Cerantola, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. Highlights ► Structural data of a complex bacterial exopolysaccharide of deep-sea origin are presented. ► This exopolysaccharide, named deepsane, is commercialised in cosmetics. ► Structural similarities with other exopolysaccharide-producing marine Alteromonas strains are reported.
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Structural data on a bacterial exopolysaccharide produced by a deep-sea Alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Stephane Cerantola, Jacqueline Ratiskol, Corinne Sinquin, David Ropartz, S. Colliec-jouault, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. (C) 2012 Elsevier Ltd. All rights reserved.
David Ropartz - One of the best experts on this subject based on the ideXlab platform.
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structural data on a bacterial exopolysaccharide produced by a deep sea alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Le T Costaouec, Jacqueline Ratiskol, Corinne Sinquin, Sylvia Colliecjouault, David Ropartz, Stephane Cerantola, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. Highlights ► Structural data of a complex bacterial exopolysaccharide of deep-sea origin are presented. ► This exopolysaccharide, named deepsane, is commercialised in cosmetics. ► Structural similarities with other exopolysaccharide-producing marine Alteromonas strains are reported.
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Structural data on a bacterial exopolysaccharide produced by a deep-sea Alteromonas macleodii strain
Carbohydrate Polymers, 2012Co-Authors: Stephane Cerantola, Jacqueline Ratiskol, Corinne Sinquin, David Ropartz, S. Colliec-jouault, Claire BoissetAbstract:Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative Exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this original and complex bacterial exopolysaccharide and highlights some structural similarities with other known EPS produced by marine Alteromonas strains. (C) 2012 Elsevier Ltd. All rights reserved.