The Experts below are selected from a list of 5307 Experts worldwide ranked by ideXlab platform
Robert J. Ruben - One of the best experts on this subject based on the ideXlab platform.
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acute streptococcus pneumoniae meningogenic labyrinthitis an Experimental Guinea Pig model and literature review
Archives of Otolaryngology-head & Neck Surgery, 1994Co-Authors: Andrew L Blank, Thomas R Vandewater, Gustave L. Davis, Robert J. RubenAbstract:Objective: To create an Experimental model of Streptococcus pneumoniae type 3 meningogenic labyrinthitis (a leading cause of deafness) similar to that in human disease. Design: Cohort analytic study of Guinea Pigs that were inoculated intrathecally with varying dilutions of S pneumoniae type 3; the progress of the disease was compared with that in saline solution–inoculated control animals. Subjects: Healthy adult Hartley Guinea Pigs without clinical evidence of middle ear disease that were conveniently sampled. Interventions: Intrathecal inoculation of 10 4 to 10 6 colony-forming units of S pneumoniae type 3 into 13 Guinea Pigs; signs and symptoms of meningitis/labyrinthitis were observed for 15 days and compared with those in two saline solution–inoculated control animals. Main Outcome Measures: Morbidity—labyrinthitis, meningitis; end point—death. Results: The 10 4 to 10 6 colony-forming units of S pneumoniae type 3 caused inflammation that extended from the meninges to the inner ear via the cochlear aqueduct within 3 days after inoculation; a dose of 10 7 killed animals within 12 hours after inoculation. Three of five animals that were inoculated with a 10 6 dose died 3 days after inoculation; two of three animals that were inoculated with a 10 5 dose lived to 15 days after inoculation. One of two animals that were inoculated with a 10 4 dose did not become infected. Inflammation extended to the middle ear by round-window destruction. Reactive bone formation simulated labyrinthine osteosclerosis. Observers assessed histologic slides "blindly." Conclusion: Guinea Pigs can survive 15 days after intrathecal inoculation of a 10 5 dose, with morphologic features similar to those in human disease. This is an effective model for this study of meningogenic labyrinthitis. (Arch Otolaryngol Head Neck Surg. 1994;120:1342-1346)
D M Saunte - One of the best experts on this subject based on the ideXlab platform.
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Experimental Guinea Pig model of dermatophytosis a simple and useful tool for the evaluation of new diagnostics and antifungals
Medical Mycology, 2008Co-Authors: D M Saunte, J P Hasselby, Anna Brillowskadabrowska, Niels Frimodtmoller, E Svejgaard, D Linnemann, Sanne Sogaard Nielsen, Merete Haedersdal, Maiken Cavling ArendrupAbstract:The aim of this study was to establish a simple Guinea Pig model for the purpose of evaluating diagnostic principles and treatment modalities for dermatophytic infections. The following variables were evaluated; pre-treatment of the skin by shaving versus tape stripping, Microsporum canis or Trichophyton mentagrophytes test strains as etiologic agents, differences in inoculum concentrations, and inoculation with and without occlusion. The course of infection was evaluated clinically by redness and lesion scores and mycologically by microscopy, culture, and histopathology. The applicability of the model was evaluated with a recently developed diagnostic pan-dermatophyte PCR and antifungal treatment was tested with an oral solution of itraconazole, 10 mg/kg, once daily during days 3–14 of the test period. Pre-treatment of the skin with a manual razor was for practical reasons preferable to tape stripping. Inoculation under occlusion showed no advantage in the establishment of Experimental infections. Infect...
Andrew L Blank - One of the best experts on this subject based on the ideXlab platform.
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acute streptococcus pneumoniae meningogenic labyrinthitis an Experimental Guinea Pig model and literature review
Archives of Otolaryngology-head & Neck Surgery, 1994Co-Authors: Andrew L Blank, Thomas R Vandewater, Gustave L. Davis, Robert J. RubenAbstract:Objective: To create an Experimental model of Streptococcus pneumoniae type 3 meningogenic labyrinthitis (a leading cause of deafness) similar to that in human disease. Design: Cohort analytic study of Guinea Pigs that were inoculated intrathecally with varying dilutions of S pneumoniae type 3; the progress of the disease was compared with that in saline solution–inoculated control animals. Subjects: Healthy adult Hartley Guinea Pigs without clinical evidence of middle ear disease that were conveniently sampled. Interventions: Intrathecal inoculation of 10 4 to 10 6 colony-forming units of S pneumoniae type 3 into 13 Guinea Pigs; signs and symptoms of meningitis/labyrinthitis were observed for 15 days and compared with those in two saline solution–inoculated control animals. Main Outcome Measures: Morbidity—labyrinthitis, meningitis; end point—death. Results: The 10 4 to 10 6 colony-forming units of S pneumoniae type 3 caused inflammation that extended from the meninges to the inner ear via the cochlear aqueduct within 3 days after inoculation; a dose of 10 7 killed animals within 12 hours after inoculation. Three of five animals that were inoculated with a 10 6 dose died 3 days after inoculation; two of three animals that were inoculated with a 10 5 dose lived to 15 days after inoculation. One of two animals that were inoculated with a 10 4 dose did not become infected. Inflammation extended to the middle ear by round-window destruction. Reactive bone formation simulated labyrinthine osteosclerosis. Observers assessed histologic slides "blindly." Conclusion: Guinea Pigs can survive 15 days after intrathecal inoculation of a 10 5 dose, with morphologic features similar to those in human disease. This is an effective model for this study of meningogenic labyrinthitis. (Arch Otolaryngol Head Neck Surg. 1994;120:1342-1346)
Maiken Cavling Arendrup - One of the best experts on this subject based on the ideXlab platform.
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Experimental Guinea Pig model of dermatophytosis a simple and useful tool for the evaluation of new diagnostics and antifungals
Medical Mycology, 2008Co-Authors: D M Saunte, J P Hasselby, Anna Brillowskadabrowska, Niels Frimodtmoller, E Svejgaard, D Linnemann, Sanne Sogaard Nielsen, Merete Haedersdal, Maiken Cavling ArendrupAbstract:The aim of this study was to establish a simple Guinea Pig model for the purpose of evaluating diagnostic principles and treatment modalities for dermatophytic infections. The following variables were evaluated; pre-treatment of the skin by shaving versus tape stripping, Microsporum canis or Trichophyton mentagrophytes test strains as etiologic agents, differences in inoculum concentrations, and inoculation with and without occlusion. The course of infection was evaluated clinically by redness and lesion scores and mycologically by microscopy, culture, and histopathology. The applicability of the model was evaluated with a recently developed diagnostic pan-dermatophyte PCR and antifungal treatment was tested with an oral solution of itraconazole, 10 mg/kg, once daily during days 3–14 of the test period. Pre-treatment of the skin with a manual razor was for practical reasons preferable to tape stripping. Inoculation under occlusion showed no advantage in the establishment of Experimental infections. Infect...
Thomas R Vandewater - One of the best experts on this subject based on the ideXlab platform.
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acute streptococcus pneumoniae meningogenic labyrinthitis an Experimental Guinea Pig model and literature review
Archives of Otolaryngology-head & Neck Surgery, 1994Co-Authors: Andrew L Blank, Thomas R Vandewater, Gustave L. Davis, Robert J. RubenAbstract:Objective: To create an Experimental model of Streptococcus pneumoniae type 3 meningogenic labyrinthitis (a leading cause of deafness) similar to that in human disease. Design: Cohort analytic study of Guinea Pigs that were inoculated intrathecally with varying dilutions of S pneumoniae type 3; the progress of the disease was compared with that in saline solution–inoculated control animals. Subjects: Healthy adult Hartley Guinea Pigs without clinical evidence of middle ear disease that were conveniently sampled. Interventions: Intrathecal inoculation of 10 4 to 10 6 colony-forming units of S pneumoniae type 3 into 13 Guinea Pigs; signs and symptoms of meningitis/labyrinthitis were observed for 15 days and compared with those in two saline solution–inoculated control animals. Main Outcome Measures: Morbidity—labyrinthitis, meningitis; end point—death. Results: The 10 4 to 10 6 colony-forming units of S pneumoniae type 3 caused inflammation that extended from the meninges to the inner ear via the cochlear aqueduct within 3 days after inoculation; a dose of 10 7 killed animals within 12 hours after inoculation. Three of five animals that were inoculated with a 10 6 dose died 3 days after inoculation; two of three animals that were inoculated with a 10 5 dose lived to 15 days after inoculation. One of two animals that were inoculated with a 10 4 dose did not become infected. Inflammation extended to the middle ear by round-window destruction. Reactive bone formation simulated labyrinthine osteosclerosis. Observers assessed histologic slides "blindly." Conclusion: Guinea Pigs can survive 15 days after intrathecal inoculation of a 10 5 dose, with morphologic features similar to those in human disease. This is an effective model for this study of meningogenic labyrinthitis. (Arch Otolaryngol Head Neck Surg. 1994;120:1342-1346)
