The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
H R Pappu - One of the best experts on this subject based on the ideXlab platform.
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differential expression of tomato spotted wilt virus derived viral small rnas in infected commercial and Experimental Host plants
PLOS ONE, 2013Co-Authors: Neena Mitter, Vikas Koundal, Sarah Williams, H R PappuAbstract:Background:Viral small RNAs (vsiRNAs) in the infected Host can be generated from viral double-stranded RNA replicative intermediates, self-complementary regions of the viral genome or from the action of Host RNA-dependent RNA polymerases on viral templates. The vsiRNA abundance and profile as well as the endogenous small RNA population can vary between different Hosts infected by the same virus influencing viral pathogenicity and Host response. There are no reports on the analysis of vsiRNAs of Tomato spotted wilt virus (TSWV), a segmented negativestranded RNA virusin thefamilyBunyaviridae, with two of its gene segments showing ambisense gene arrangement. The virus causes significant economic losses to numerous field and horticultural crops worldwide.Principal Findings:Tomato spotted wilt virus (TSWV)-specific vsiRNAs were characterized by deep sequencing in virus-infected Experimental Host Nicotiana benthamiana and a commercial, susceptible Host tomato. The total small (s) RNA reads in TSWV-infected tomato sample showed relatively equal distribution of 21, 22 and 24 nt, whereas N. benthamiana sample was dominated by 24 nt total sRNAs. The number of vsiRNA reads detected in tomato was many a magnitude (~350:1) higher than those found in N. benthamiana, however the profile of vsiRNAs in terms of relative abundance 21, 22 and 24 nt class size was similar in both the Hosts. Maximum vsiRNA reads were obtained for the M RNA segment of TSWV while the largest L RNA segment had the least number of vsiRNAs in both tomato and N. benthamiana. Only the silencing suppressor, NSs, of TSWV recorded higher antisense vsiRNA with respect to the coding frame among all the genes of TSWV.Significance:Details of the origin, distribution and abundance of TSWV vsiRNAs could be useful in designing efficient targets for exploiting RNA interference for virus resistance. It also has major implications toward our understanding of the differential processing of vsiRNAs in antiviral defense and viral pathogenicity.
R M Krol - One of the best experts on this subject based on the ideXlab platform.
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Experimental Host range and histopathology of white spot syndrome virus wssv infection in shrimp prawns crabs and lobsters from india
Journal of Fish Diseases, 1999Co-Authors: K V Rajendran, K K Vijayan, T C Santiago, R M KrolAbstract:Experimental studies were conducted by injecting or feeding white spot syndrome virus (WSSV) derived from infected shrimp, Penaeus monodon (Fabricius), collected from the south-east coast of India, to five species of shrimp, two species of freshwater prawns, four species of crabs and three species of lobsters. All species examined were susceptible to the virus. Experimental infections in the shrimp had the same clinical symptoms and histopathological characteristics as in naturally infected P. monodon. A cumulative mortality of 100% was observed within 5–7 days in shrimp injected with WSSV and 7–9 days in shrimp fed with infected tissue. Two species of mud crab, Scylla sp., survived the infection for 30 days without any clinical symptoms. All three species of lobsters, Panulirus sp., and the freshwater prawn, Macrobrachium rosenbergii (De Man), survived the infection for 70 days without clinical symptoms. However, bioassay and histology using healthy P. monodon revealed that crabs, prawns and lobsters may act as asymptomatic carriers/reservoir Hosts of WSSV. This is the first report to suggest the carrier/reservoir capacity of these Hosts through histological and bioassay evidences. Ultrastructural details of the virus in Experimentally infected shrimp, P. vannamei, (Boone), were also studied.
Neena Mitter - One of the best experts on this subject based on the ideXlab platform.
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differential expression of tomato spotted wilt virus derived viral small rnas in infected commercial and Experimental Host plants
PLOS ONE, 2013Co-Authors: Neena Mitter, Vikas Koundal, Sarah Williams, H R PappuAbstract:Background:Viral small RNAs (vsiRNAs) in the infected Host can be generated from viral double-stranded RNA replicative intermediates, self-complementary regions of the viral genome or from the action of Host RNA-dependent RNA polymerases on viral templates. The vsiRNA abundance and profile as well as the endogenous small RNA population can vary between different Hosts infected by the same virus influencing viral pathogenicity and Host response. There are no reports on the analysis of vsiRNAs of Tomato spotted wilt virus (TSWV), a segmented negativestranded RNA virusin thefamilyBunyaviridae, with two of its gene segments showing ambisense gene arrangement. The virus causes significant economic losses to numerous field and horticultural crops worldwide.Principal Findings:Tomato spotted wilt virus (TSWV)-specific vsiRNAs were characterized by deep sequencing in virus-infected Experimental Host Nicotiana benthamiana and a commercial, susceptible Host tomato. The total small (s) RNA reads in TSWV-infected tomato sample showed relatively equal distribution of 21, 22 and 24 nt, whereas N. benthamiana sample was dominated by 24 nt total sRNAs. The number of vsiRNA reads detected in tomato was many a magnitude (~350:1) higher than those found in N. benthamiana, however the profile of vsiRNAs in terms of relative abundance 21, 22 and 24 nt class size was similar in both the Hosts. Maximum vsiRNA reads were obtained for the M RNA segment of TSWV while the largest L RNA segment had the least number of vsiRNAs in both tomato and N. benthamiana. Only the silencing suppressor, NSs, of TSWV recorded higher antisense vsiRNA with respect to the coding frame among all the genes of TSWV.Significance:Details of the origin, distribution and abundance of TSWV vsiRNAs could be useful in designing efficient targets for exploiting RNA interference for virus resistance. It also has major implications toward our understanding of the differential processing of vsiRNAs in antiviral defense and viral pathogenicity.
K V Rajendran - One of the best experts on this subject based on the ideXlab platform.
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Experimental Host range and histopathology of white spot syndrome virus wssv infection in shrimp prawns crabs and lobsters from india
Journal of Fish Diseases, 1999Co-Authors: K V Rajendran, K K Vijayan, T C Santiago, R M KrolAbstract:Experimental studies were conducted by injecting or feeding white spot syndrome virus (WSSV) derived from infected shrimp, Penaeus monodon (Fabricius), collected from the south-east coast of India, to five species of shrimp, two species of freshwater prawns, four species of crabs and three species of lobsters. All species examined were susceptible to the virus. Experimental infections in the shrimp had the same clinical symptoms and histopathological characteristics as in naturally infected P. monodon. A cumulative mortality of 100% was observed within 5–7 days in shrimp injected with WSSV and 7–9 days in shrimp fed with infected tissue. Two species of mud crab, Scylla sp., survived the infection for 30 days without any clinical symptoms. All three species of lobsters, Panulirus sp., and the freshwater prawn, Macrobrachium rosenbergii (De Man), survived the infection for 70 days without clinical symptoms. However, bioassay and histology using healthy P. monodon revealed that crabs, prawns and lobsters may act as asymptomatic carriers/reservoir Hosts of WSSV. This is the first report to suggest the carrier/reservoir capacity of these Hosts through histological and bioassay evidences. Ultrastructural details of the virus in Experimentally infected shrimp, P. vannamei, (Boone), were also studied.
Vikas Koundal - One of the best experts on this subject based on the ideXlab platform.
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differential expression of tomato spotted wilt virus derived viral small rnas in infected commercial and Experimental Host plants
PLOS ONE, 2013Co-Authors: Neena Mitter, Vikas Koundal, Sarah Williams, H R PappuAbstract:Background:Viral small RNAs (vsiRNAs) in the infected Host can be generated from viral double-stranded RNA replicative intermediates, self-complementary regions of the viral genome or from the action of Host RNA-dependent RNA polymerases on viral templates. The vsiRNA abundance and profile as well as the endogenous small RNA population can vary between different Hosts infected by the same virus influencing viral pathogenicity and Host response. There are no reports on the analysis of vsiRNAs of Tomato spotted wilt virus (TSWV), a segmented negativestranded RNA virusin thefamilyBunyaviridae, with two of its gene segments showing ambisense gene arrangement. The virus causes significant economic losses to numerous field and horticultural crops worldwide.Principal Findings:Tomato spotted wilt virus (TSWV)-specific vsiRNAs were characterized by deep sequencing in virus-infected Experimental Host Nicotiana benthamiana and a commercial, susceptible Host tomato. The total small (s) RNA reads in TSWV-infected tomato sample showed relatively equal distribution of 21, 22 and 24 nt, whereas N. benthamiana sample was dominated by 24 nt total sRNAs. The number of vsiRNA reads detected in tomato was many a magnitude (~350:1) higher than those found in N. benthamiana, however the profile of vsiRNAs in terms of relative abundance 21, 22 and 24 nt class size was similar in both the Hosts. Maximum vsiRNA reads were obtained for the M RNA segment of TSWV while the largest L RNA segment had the least number of vsiRNAs in both tomato and N. benthamiana. Only the silencing suppressor, NSs, of TSWV recorded higher antisense vsiRNA with respect to the coding frame among all the genes of TSWV.Significance:Details of the origin, distribution and abundance of TSWV vsiRNAs could be useful in designing efficient targets for exploiting RNA interference for virus resistance. It also has major implications toward our understanding of the differential processing of vsiRNAs in antiviral defense and viral pathogenicity.
