Experimental Infection

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O Papadopoulos - One of the best experts on this subject based on the ideXlab platform.

P Tsakos - One of the best experts on this subject based on the ideXlab platform.

N Papaioannou - One of the best experts on this subject based on the ideXlab platform.

  • the induction of lymphoid follicle like structures in the ovine teat duct following Experimental Infection with mannheimia haemolytica
    Veterinary Journal, 2010
    Co-Authors: I A Fragkou, N Papaioannou, M P Dagleish, P J Cripps, C M Boscos, H N Ververidis, D C Orfanou, N Solomakos, J Finlayson, A Govaris
    Abstract:

    Abstract The objectives of this study were to investigate the early stages of Experimental Infection of the ovine mammary gland with Mannheimia haemolytica and to identify the lymphocyte subsets accumulating at the teat duct. M. haemolytica was inoculated into one teat of each of 25 ewes and clinical, bacteriological, cytological, haematological, physicochemical, gross pathological, histopathological and immunohistochemical examinations were carried out. Clinical signs of inflammation were evident by 8 h but had subsided 2 days after challenge. Polymorphonuclear neutrophils (PMN) predominated in milk films up to 1 day following challenge, but the proportion of lymphocytes and macrophages progressively increased thereafter. Total blood leucocyte counts decreased immediately after challenge and then rose until 1 day after challenge with immature PMNs comprising >3% of the total. The pH of the mammary secretions from the challenged side was increased (>7.0). Focal lymphoid accumulations were observed in the lamina propria at the junction of the teat duct and cistern, including CD79 + , CD3 + and γδ T cells, CD68 + and MHC-II + cells with a particular increase in the numbers of CD8 + T cells from days 3 to 5 after challenge. The findings suggest that these organised lymphoid structures are inducible and contribute to the defence of the infected teat when the PMN-macrophage response is overwhelmed.

  • corrigendum to Experimental Infection of pregnant ewes with enteric and abortion source chlamydophila abortus vet microbiol 82 2001 285 291
    Veterinary Microbiology, 2002
    Co-Authors: P Tsakos, Evangelia Vretou, Franco Guscetti, E H Chowdhury, N Papaioannou, Victoria I. Siarkou, O Papadopoulos
    Abstract:

    Corrigendum to ‘‘Experimental Infection of pregnant ewes with enteric and abortion-source Chlamydophila abortus’’ [Vet. Microbiol. 82 (2001) 285–291] P. Tsakos, V. Siarkou, F. Guscetti, E.H. Chowdhury, N. Papaioannou, E. Vretou, O. Papadopoulos Faculty of Veterinary Medicine, Laboratory of Microbiology and Infectious Diseases, Aristotle University, 54 006 Thessaloniki, Greece Institute of Veterinary Pathology, University of Zurich, CH-8057 Zurich, Switzerland Faculty of Veterinary Medicine, Laboratory of Pathology, Aristotle University, 54 006 Thessaloniki, Greece Department of Biotechnology, Hellenic Pasteur Institute, 115 21 Athens, Greece

  • Corrigendum to “Experimental Infection of pregnant ewes with enteric and abortion-source Chlamydophila abortus” [Vet. Microbiol. 82 (2001) 285–291]☆
    Veterinary Microbiology, 2002
    Co-Authors: P Tsakos, Evangelia Vretou, Franco Guscetti, E H Chowdhury, N Papaioannou, Victoria I. Siarkou, O Papadopoulos
    Abstract:

    Corrigendum to ‘‘Experimental Infection of pregnant ewes with enteric and abortion-source Chlamydophila abortus’’ [Vet. Microbiol. 82 (2001) 285–291] P. Tsakos, V. Siarkou, F. Guscetti, E.H. Chowdhury, N. Papaioannou, E. Vretou, O. Papadopoulos Faculty of Veterinary Medicine, Laboratory of Microbiology and Infectious Diseases, Aristotle University, 54 006 Thessaloniki, Greece Institute of Veterinary Pathology, University of Zurich, CH-8057 Zurich, Switzerland Faculty of Veterinary Medicine, Laboratory of Pathology, Aristotle University, 54 006 Thessaloniki, Greece Department of Biotechnology, Hellenic Pasteur Institute, 115 21 Athens, Greece

Franco Guscetti - One of the best experts on this subject based on the ideXlab platform.

Evangelia Vretou - One of the best experts on this subject based on the ideXlab platform.