The Experts below are selected from a list of 180480 Experts worldwide ranked by ideXlab platform
Liu Ying - One of the best experts on this subject based on the ideXlab platform.
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Construction of plant male-sterility and female & male-sterility Expression Vector
Journal of Central South University of Forestry & Technology, 2010Co-Authors: Liu YingAbstract:This study created male-sterility plant Expression Vector using the anther tapetum specific BoA9 gene promoter and cell toxin gene BoCysP1.And the female-sterility Expression Vector was also constructed through the pistil-specific Expression promoter—S12-RNase gene promoter and BoCysP1 gene.In the end male female sterility plant Expression Vector BoA9pro-CysP1-PpS12pro-CysP1-pBI101.2 was constructed by double digestion and connection of the above two Vectors.The female-sterility plant Expression Vector and male female-sterility plant Expression Vector can used in genetically modification of female plants of dioecism and monoecious garden green plants in order to obtain new landscaping seedless fruit varieties and stop the phenomenon of flying lint effectively.
Yuxin Tang - One of the best experts on this subject based on the ideXlab platform.
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Construction of TDRG1 shRNA Expression Vector and interfering effect of TDRG1 shRNA Expression Vector on NTERA-2 cells
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2012Co-Authors: Sheng-lin Peng, Jianfu Yang, Houyang Chen, Xiaoliang Guo, Hua-bo Zhou, Yu Gan, Xianzhen Jiang, Yuxin TangAbstract:Objective: To construct short hairpin RNA interfering Expression Vector of TDRG1,and detect the
Tian Xia - One of the best experts on this subject based on the ideXlab platform.
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Cloning,Expression and Identification of Mycobacterium Tuberculosis Protein MPT83 in Eukaryotic Expression Vector and Prokaryotic Expression Vector
Progress in Veterinary Medicine, 2003Co-Authors: Tian XiaAbstract:Cloning,identification and Expression of antigen MPT83 from Mycobacterium Tuberculosis play a role in diagnosis of tuberculosis,preparation of subunit vaccine and DNA vaccine and application of those vaccines. We amplified gene mpt83 based on the genome DNA sequence of H37RV by PCR and then cloned them into eukaryotic Expression Vector pJW4303 and prokaryotic Expression Vector pET22b(+) after digested with restriction endonuclease corresponding to the enzyme sites on the Vectors. We named those constructions 83eu and MPT83. After identified correctly by digestion with restriction endonuclease and sequencing,the Vectors 83eu transferred COS-7 cells and MPT83 transformed E.coli BL21(DE3)plysS respectively. Result:SDS-PAGE and Western blotting analysis of the protein induced by IPTG after the prokaryotic Expression Vector MPT83 transformed BL21(DE3)plysS demonstrated a specific protein about 26KD;western blotting also showed a specific protein reacting actively with anti-tuberculosis serum after the eukaryotic Expression Vector 83eu transferred COS-7 cells.
Zhao Chuanji - One of the best experts on this subject based on the ideXlab platform.
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construction of Expression Vector for candidate gene of bacterial blight resistance gene xa31 t in rice
Journal of Anhui Agricultural Sciences, 2013Co-Authors: Zhao ChuanjiAbstract:[Objective] The paper was to construct the Expression Vector for candidate gene of the bacterial blight resistance gene Xa31(t) in rice.[Method] A 10.6 kb-length candidate gene was amplified form the genomic DNA of bacterial blight resistance rice cultivar 'Za Chang Long'(ZCL) by long-range PCR,and the gene was cloned into plant Expression Vector pCAMBIA1300 by Asc I digestion.The positive clones were detected by plasmid PCR,restriction enzyme digestion and sequencing.[Result] The Expression Vector for candidate gene of the bacterial blight resistance gene Xa31(t) in rice was constructed successfully.[Conclusion] The research laid a foundation for the further study of the function of Xa31(t) gene.
Deng Hong-yu - One of the best experts on this subject based on the ideXlab platform.
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Construction of recombinant human interleukin 27 eukaryotic Expression Vector
2011Co-Authors: Deng Hong-yuAbstract:Objective To clone the gene of human interleukin 27 and construct the eukaryotic Expression Vector.Methods Total RNA was extracted from dendritic cells induced from PBMC.The gene of EBI3 and p28 were cloned by RT-PCR and double enzyme digested by Xho I and EcoR I,to construct the eukaryotic Expression Vector of human interleukin 27.Results The EBI3 and p28 sequences in the eukaryotic Expression Vector were correct.Conclusions The eukaryotic Expression Vectors of human interleukin 27 were constructed successfully.
