The Experts below are selected from a list of 90219 Experts worldwide ranked by ideXlab platform
Ting Deng - One of the best experts on this subject based on the ideXlab platform.
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dispersive matrix solid phase Extraction method coupled with high performance liquid chromatography tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute Plants and its application for geographical distribution study
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Ting Deng, Chunfeng Dua, Na Wang, Yafeng GuaAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38−6.75 pg mL–1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%−9.8% and 4.6%−17.3%, respectively. The proposed method was successfully app...
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dispersive matrix solid phase Extraction method coupled with high performance liquid chromatography tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute Plants and its application for geographical distribution study
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Ting Deng, Chunfeng Duan, Nan Wang, Yafeng GuanAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38-6.75 pg mL-1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%-9.8% and 4.6%-17.3%, respectively. The proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram Arabidopsis thaliana seedlings (0.5 mg). Finally, the geographical distribution of five endogenous brassinosteroids of Brassica napus L. oilseed rape in different provinces of origin in the Yangtze River basin was described.
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Dispersive Matrix Solid-Phase Extraction Method Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry for Ultrasensitive Quantification of Endogenous Brassinosteroids in Minute Plants and Its Application for Geographical Distribution Study
2019Co-Authors: Ting Deng, Chunfeng Duan, Nan Wang, Yafeng GuanAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38−6.75 pg mL–1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%−9.8% and 4.6%−17.3%, respectively. The proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram Arabidopsis thaliana seedlings (0.5 mg). Finally, the geographical distribution of five endogenous brassinosteroids of Brassica napus L. oilseed rape in different provinces of origin in the Yangtze River basin was described
Yafeng Guan - One of the best experts on this subject based on the ideXlab platform.
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dispersive matrix solid phase Extraction method coupled with high performance liquid chromatography tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute Plants and its application for geographical distribution study
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Ting Deng, Chunfeng Duan, Nan Wang, Yafeng GuanAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38-6.75 pg mL-1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%-9.8% and 4.6%-17.3%, respectively. The proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram Arabidopsis thaliana seedlings (0.5 mg). Finally, the geographical distribution of five endogenous brassinosteroids of Brassica napus L. oilseed rape in different provinces of origin in the Yangtze River basin was described.
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Dispersive Matrix Solid-Phase Extraction Method Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry for Ultrasensitive Quantification of Endogenous Brassinosteroids in Minute Plants and Its Application for Geographical Distribution Study
2019Co-Authors: Ting Deng, Chunfeng Duan, Nan Wang, Yafeng GuanAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38−6.75 pg mL–1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%−9.8% and 4.6%−17.3%, respectively. The proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram Arabidopsis thaliana seedlings (0.5 mg). Finally, the geographical distribution of five endogenous brassinosteroids of Brassica napus L. oilseed rape in different provinces of origin in the Yangtze River basin was described
Yafeng Gua - One of the best experts on this subject based on the ideXlab platform.
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dispersive matrix solid phase Extraction method coupled with high performance liquid chromatography tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute Plants and its application for geographical distribution study
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Ting Deng, Chunfeng Dua, Na Wang, Yafeng GuaAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38−6.75 pg mL–1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%−9.8% and 4.6%−17.3%, respectively. The proposed method was successfully app...
Nan Wang - One of the best experts on this subject based on the ideXlab platform.
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dispersive matrix solid phase Extraction method coupled with high performance liquid chromatography tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute Plants and its application for geographical distribution study
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Ting Deng, Chunfeng Duan, Nan Wang, Yafeng GuanAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38-6.75 pg mL-1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%-9.8% and 4.6%-17.3%, respectively. The proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram Arabidopsis thaliana seedlings (0.5 mg). Finally, the geographical distribution of five endogenous brassinosteroids of Brassica napus L. oilseed rape in different provinces of origin in the Yangtze River basin was described.
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Dispersive Matrix Solid-Phase Extraction Method Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry for Ultrasensitive Quantification of Endogenous Brassinosteroids in Minute Plants and Its Application for Geographical Distribution Study
2019Co-Authors: Ting Deng, Chunfeng Duan, Nan Wang, Yafeng GuanAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38−6.75 pg mL–1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%−9.8% and 4.6%−17.3%, respectively. The proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram Arabidopsis thaliana seedlings (0.5 mg). Finally, the geographical distribution of five endogenous brassinosteroids of Brassica napus L. oilseed rape in different provinces of origin in the Yangtze River basin was described
Na Wang - One of the best experts on this subject based on the ideXlab platform.
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dispersive matrix solid phase Extraction method coupled with high performance liquid chromatography tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute Plants and its application for geographical distribution study
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Ting Deng, Chunfeng Dua, Na Wang, Yafeng GuaAbstract:An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute Plants was developed based on dispersive matrix solid-phase Extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase Extraction, Plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding Extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase Extraction were compared and discussed for Plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38−6.75 pg mL–1 for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%−9.8% and 4.6%−17.3%, respectively. The proposed method was successfully app...
