The Experts below are selected from a list of 42 Experts worldwide ranked by ideXlab platform
X. De Lamballerie - One of the best experts on this subject based on the ideXlab platform.
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ColtiViruses and seadornaViruses in North America, Europe, and Asia.
Emerging Infectious Diseases, 2005Co-Authors: Houssam Attoui, Philippe De Micco, Fauziah Mohd Jaafar, X. De LamballerieAbstract:ColtiViruses are tickborne Viruses of the genus ColtiVirus. The type species, Colorado tick fever Virus (from North America), has been isolated from patients with flulike syndromes, meningitis, encephalitis, and other severe complications. Another coltiVirus, Eyach Virus, has been isolated from ticks in France and Germany and incriminated in febrile illnesses and neurologic syndromes. SeadornaViruses are endemic in Southeast Asia, particularly Indonesia and China. The prototype Virus of the genus, Banna Virus (BAV), has been isolated from many mosquito species, humans with encephalitis, pigs, and cattle. Two other seadornaViruses, Kadipiro and Liao Ning, were isolated only from mosquitoes. The epidemiology of seadornaViruses remains poorly documented. Evidence suggests that BAV is responsible for encephalitis in humans. Infection with BAV may be underreported because it circulates in regions with a high incidence of Japanese encephalitis and could be misdiagnosed as this disease.
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Recombinant VP6-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Eyach Virus (genus ColtiVirus)
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2004Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Philippe De Micco, X. De LamballerieAbstract:Background: Eyach Virus (EYAV) is a tick-borne Virus belonging to genus ColtiVirus, family Reoviridae. It was isolated in Germany and France and has been suspected to be responsible for neurological diseases in humans. To date, there has been no relatively rapid and relatively specific serological assay for EYAV. Objectives: To develop an ELISA for EYAV, suitable for epidemiological and/or diagnostic purposes. This ELISA should allow to distinguish between infections with EYAV and the related Colorado tick fever Virus (CTFV). Study Design: VP6, VP7 and VP12 of Eyach Virus (the three proteins most divergent between EYAV and CTFV) were expressed in bacteria using the pGEX-4T-2 vector. A partial sequence of VP6 (designated pVP6) was chosen to develop an ELISA for detecting anti-EYAV IgG antibodies in serum. This choice was based on two observations: (i) the homologous VP7 protein of CTFV was successfully used as a target for detecting antibodies to CTFV (the VP7 showed the highest reactivity to an anti-CTFV antibody among all CTFV expressed proteins); (ii) to distinguish infection with EYAV from a CTFV infection: the expressed sequence was chosen within a region which is highly divergent (49% of amino acid identity) from the homologous VP7 sequence of CTFV. Results and conclusions: pVP6 was shown to be the most reactive among the three expressed proteins. The elaborated pVP6 ELISA was evaluated with 340 sera of French blood donors, and found to exhibit a specificity of 100% (no false positives). Furthermore, no cross reaction was detected with antibody to CTFV, thus permitting us to distinguish between infections by either Virus. The use of this recombinant protein for serological assays is a good alternative to the use of native EYAV antigen due to the extremely low productivity of the Virus in cell culture, and the requirement for suckling mice. This ELISA will be useful to clarify the epidemiological status and the suspected pathogenicity of the Virus.
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Recombinant VP7-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies to Colorado Tick Fever Virus
Journal of Clinical Microbiology, 2003Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Philippe De Micco, Pierre Gallian, Philippe Biagini, Jean-françois Cantaloube, X. De LamballerieAbstract:VP6, VP7, VP9, VP10, VP11, and VP12 of Colorado tick fever Virus (CTF Virus), a Virus member of the genus ColtiVirus, family Reoviridae, were expressed in bacteria with the pGEX-4T-2 vector. A partial sequence of VP7 (designated pVP7) was chosen to elaborate an enzyme-linked immunosorbent assay (ELISA) for detecting anti-CTF Virus immunoglobulin G (IgG) antibodies in humans. This was based on two observations: (i) among all expressed proteins, pVP7 showed the highest immunoreactivity to an anti-CTF Virus hyperimmune ascitic fluid; (ii) to provide the highest selectivity of antibody detection, the expressed sequence was chosen within a region which is highly divergent (49% amino acid identity) from the homologous sequence of another coltiVirus, the Eyach Virus. The pVP7 ELISA was evaluated with 368 serum samples from French blood donors and found to provide 98.1% specificity. Assays with the Calisher set of human serum samples, positive for anti-CTF Virus antibodies (C. H. Calisher, J. D. Poland, S. B. Calisher, and L. A Warmoth, J. Clin. Microbiol. 22:84-88, 1985), showed that the pVP7 ELISA provided 100% sensitivity for the tested population. After elaboration of recombinant-protein-based ELISAs for diagnosis of infections with members of the viral genera OrbiVirus, OrthoreoVirus, and RotaVirus, it was shown that a recombinant protein could be used to detect antibodies to the human pathogen Colorado tick fever Virus.
Fauziah Mohd Jaafar - One of the best experts on this subject based on the ideXlab platform.
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ColtiViruses and seadornaViruses in North America, Europe, and Asia.
Emerging Infectious Diseases, 2005Co-Authors: Houssam Attoui, Philippe De Micco, Fauziah Mohd Jaafar, X. De LamballerieAbstract:ColtiViruses are tickborne Viruses of the genus ColtiVirus. The type species, Colorado tick fever Virus (from North America), has been isolated from patients with flulike syndromes, meningitis, encephalitis, and other severe complications. Another coltiVirus, Eyach Virus, has been isolated from ticks in France and Germany and incriminated in febrile illnesses and neurologic syndromes. SeadornaViruses are endemic in Southeast Asia, particularly Indonesia and China. The prototype Virus of the genus, Banna Virus (BAV), has been isolated from many mosquito species, humans with encephalitis, pigs, and cattle. Two other seadornaViruses, Kadipiro and Liao Ning, were isolated only from mosquitoes. The epidemiology of seadornaViruses remains poorly documented. Evidence suggests that BAV is responsible for encephalitis in humans. Infection with BAV may be underreported because it circulates in regions with a high incidence of Japanese encephalitis and could be misdiagnosed as this disease.
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Recombinant VP6-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Eyach Virus (genus ColtiVirus)
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2004Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Philippe De Micco, X. De LamballerieAbstract:Background: Eyach Virus (EYAV) is a tick-borne Virus belonging to genus ColtiVirus, family Reoviridae. It was isolated in Germany and France and has been suspected to be responsible for neurological diseases in humans. To date, there has been no relatively rapid and relatively specific serological assay for EYAV. Objectives: To develop an ELISA for EYAV, suitable for epidemiological and/or diagnostic purposes. This ELISA should allow to distinguish between infections with EYAV and the related Colorado tick fever Virus (CTFV). Study Design: VP6, VP7 and VP12 of Eyach Virus (the three proteins most divergent between EYAV and CTFV) were expressed in bacteria using the pGEX-4T-2 vector. A partial sequence of VP6 (designated pVP6) was chosen to develop an ELISA for detecting anti-EYAV IgG antibodies in serum. This choice was based on two observations: (i) the homologous VP7 protein of CTFV was successfully used as a target for detecting antibodies to CTFV (the VP7 showed the highest reactivity to an anti-CTFV antibody among all CTFV expressed proteins); (ii) to distinguish infection with EYAV from a CTFV infection: the expressed sequence was chosen within a region which is highly divergent (49% of amino acid identity) from the homologous VP7 sequence of CTFV. Results and conclusions: pVP6 was shown to be the most reactive among the three expressed proteins. The elaborated pVP6 ELISA was evaluated with 340 sera of French blood donors, and found to exhibit a specificity of 100% (no false positives). Furthermore, no cross reaction was detected with antibody to CTFV, thus permitting us to distinguish between infections by either Virus. The use of this recombinant protein for serological assays is a good alternative to the use of native EYAV antigen due to the extremely low productivity of the Virus in cell culture, and the requirement for suckling mice. This ELISA will be useful to clarify the epidemiological status and the suspected pathogenicity of the Virus.
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Recombinant VP7-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies to Colorado Tick Fever Virus
Journal of Clinical Microbiology, 2003Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Philippe De Micco, Pierre Gallian, Philippe Biagini, Jean-françois Cantaloube, X. De LamballerieAbstract:VP6, VP7, VP9, VP10, VP11, and VP12 of Colorado tick fever Virus (CTF Virus), a Virus member of the genus ColtiVirus, family Reoviridae, were expressed in bacteria with the pGEX-4T-2 vector. A partial sequence of VP7 (designated pVP7) was chosen to elaborate an enzyme-linked immunosorbent assay (ELISA) for detecting anti-CTF Virus immunoglobulin G (IgG) antibodies in humans. This was based on two observations: (i) among all expressed proteins, pVP7 showed the highest immunoreactivity to an anti-CTF Virus hyperimmune ascitic fluid; (ii) to provide the highest selectivity of antibody detection, the expressed sequence was chosen within a region which is highly divergent (49% amino acid identity) from the homologous sequence of another coltiVirus, the Eyach Virus. The pVP7 ELISA was evaluated with 368 serum samples from French blood donors and found to provide 98.1% specificity. Assays with the Calisher set of human serum samples, positive for anti-CTF Virus antibodies (C. H. Calisher, J. D. Poland, S. B. Calisher, and L. A Warmoth, J. Clin. Microbiol. 22:84-88, 1985), showed that the pVP7 ELISA provided 100% sensitivity for the tested population. After elaboration of recombinant-protein-based ELISAs for diagnosis of infections with members of the viral genera OrbiVirus, OrthoreoVirus, and RotaVirus, it was shown that a recombinant protein could be used to detect antibodies to the human pathogen Colorado tick fever Virus.
Houssam Attoui - One of the best experts on this subject based on the ideXlab platform.
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ColtiViruses and seadornaViruses in North America, Europe, and Asia.
Emerging Infectious Diseases, 2005Co-Authors: Houssam Attoui, Philippe De Micco, Fauziah Mohd Jaafar, X. De LamballerieAbstract:ColtiViruses are tickborne Viruses of the genus ColtiVirus. The type species, Colorado tick fever Virus (from North America), has been isolated from patients with flulike syndromes, meningitis, encephalitis, and other severe complications. Another coltiVirus, Eyach Virus, has been isolated from ticks in France and Germany and incriminated in febrile illnesses and neurologic syndromes. SeadornaViruses are endemic in Southeast Asia, particularly Indonesia and China. The prototype Virus of the genus, Banna Virus (BAV), has been isolated from many mosquito species, humans with encephalitis, pigs, and cattle. Two other seadornaViruses, Kadipiro and Liao Ning, were isolated only from mosquitoes. The epidemiology of seadornaViruses remains poorly documented. Evidence suggests that BAV is responsible for encephalitis in humans. Infection with BAV may be underreported because it circulates in regions with a high incidence of Japanese encephalitis and could be misdiagnosed as this disease.
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Tick-borne Virus diseases of human interest in Europe.
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2004Co-Authors: Rémi N. Charrel, Houssam Attoui, J.c. Clegg, Vincent Deubel, T.v. Frolova, Ernest A. Gould, T.s. Gritsun, Franz X. Heinz, Milan LabudaAbstract:Several human diseases in Europe are caused by Viruses transmitted by tick bite. These Viruses belong to the genus FlaviVirus, and include tick-borne encephalitis Virus, Omsk haemorrhagic fever Virus, louping ill Virus, Powassan Virus, NairoVirus (Crimean-Congo haemorrhagic fever Virus) and ColtiVirus (Eyach Virus). All of these Viruses cause more or less severe neurological diseases, and some are also responsible for haemorrhagic fever. The epidemiology, clinical picture and methods for diagnosis are detailed in this review. Most of these viral pathogens are classified as Biosafety Level 3 or 4 agents, and therefore some of them have been classified in Categories A–C of potential bioterrorism agents by the Centers for Disease Control and Prevention. Their ability to cause severe disease in man means that these Viruses, as well as any clinical samples suspected of containing them, must be handled with specific and stringent precautions.
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Recombinant VP6-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Eyach Virus (genus ColtiVirus)
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2004Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Philippe De Micco, X. De LamballerieAbstract:Background: Eyach Virus (EYAV) is a tick-borne Virus belonging to genus ColtiVirus, family Reoviridae. It was isolated in Germany and France and has been suspected to be responsible for neurological diseases in humans. To date, there has been no relatively rapid and relatively specific serological assay for EYAV. Objectives: To develop an ELISA for EYAV, suitable for epidemiological and/or diagnostic purposes. This ELISA should allow to distinguish between infections with EYAV and the related Colorado tick fever Virus (CTFV). Study Design: VP6, VP7 and VP12 of Eyach Virus (the three proteins most divergent between EYAV and CTFV) were expressed in bacteria using the pGEX-4T-2 vector. A partial sequence of VP6 (designated pVP6) was chosen to develop an ELISA for detecting anti-EYAV IgG antibodies in serum. This choice was based on two observations: (i) the homologous VP7 protein of CTFV was successfully used as a target for detecting antibodies to CTFV (the VP7 showed the highest reactivity to an anti-CTFV antibody among all CTFV expressed proteins); (ii) to distinguish infection with EYAV from a CTFV infection: the expressed sequence was chosen within a region which is highly divergent (49% of amino acid identity) from the homologous VP7 sequence of CTFV. Results and conclusions: pVP6 was shown to be the most reactive among the three expressed proteins. The elaborated pVP6 ELISA was evaluated with 340 sera of French blood donors, and found to exhibit a specificity of 100% (no false positives). Furthermore, no cross reaction was detected with antibody to CTFV, thus permitting us to distinguish between infections by either Virus. The use of this recombinant protein for serological assays is a good alternative to the use of native EYAV antigen due to the extremely low productivity of the Virus in cell culture, and the requirement for suckling mice. This ELISA will be useful to clarify the epidemiological status and the suspected pathogenicity of the Virus.
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Recombinant VP7-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies to Colorado Tick Fever Virus
Journal of Clinical Microbiology, 2003Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Philippe De Micco, Pierre Gallian, Philippe Biagini, Jean-françois Cantaloube, X. De LamballerieAbstract:VP6, VP7, VP9, VP10, VP11, and VP12 of Colorado tick fever Virus (CTF Virus), a Virus member of the genus ColtiVirus, family Reoviridae, were expressed in bacteria with the pGEX-4T-2 vector. A partial sequence of VP7 (designated pVP7) was chosen to elaborate an enzyme-linked immunosorbent assay (ELISA) for detecting anti-CTF Virus immunoglobulin G (IgG) antibodies in humans. This was based on two observations: (i) among all expressed proteins, pVP7 showed the highest immunoreactivity to an anti-CTF Virus hyperimmune ascitic fluid; (ii) to provide the highest selectivity of antibody detection, the expressed sequence was chosen within a region which is highly divergent (49% amino acid identity) from the homologous sequence of another coltiVirus, the Eyach Virus. The pVP7 ELISA was evaluated with 368 serum samples from French blood donors and found to provide 98.1% specificity. Assays with the Calisher set of human serum samples, positive for anti-CTF Virus antibodies (C. H. Calisher, J. D. Poland, S. B. Calisher, and L. A Warmoth, J. Clin. Microbiol. 22:84-88, 1985), showed that the pVP7 ELISA provided 100% sensitivity for the tested population. After elaboration of recombinant-protein-based ELISAs for diagnosis of infections with members of the viral genera OrbiVirus, OrthoreoVirus, and RotaVirus, it was shown that a recombinant protein could be used to detect antibodies to the human pathogen Colorado tick fever Virus.
Fabian H. Leendertz - One of the best experts on this subject based on the ideXlab platform.
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A novel ColtiVirus-related Virus isolated from free-tailed bats from Côte d’Ivoire is able to infect human cells in vitro
Virology Journal, 2017Co-Authors: Sabrina Weiss, Piotr Wojtek Dabrowski, Andreas Kurth, Siv Aina J. Leendertz, Fabian H. LeendertzAbstract:Background Zoonotic transmission events play a major role in the emergence of novel diseases. While such events are virtually impossible to predict, wildlife screening for potential emerging pathogens can be a first step. Driven by recent disease epidemics like severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Ebola, bats have gained special interest as reservoirs of emerging Viruses. Methods As part of a bigger study investigating pathogens in African bats we screened animals for the presence of known and unknown Viruses. Results We isolated and characterised a novel reoVirus from blood of free-tailed bats ( Chaereophon aloysiisabaudiae ) captured in 2006 in Côte d’Ivoire. The Virus showed closest relationship with two human pathogenic Viruses, Colorado tick fever Virus and Eyach Virus, and was able to infect various human cell lines in vitro. Conclusion The study shows the presence of a coltiVirus-related Virus in bats from Sub-Sahara Africa. Serological studies could help to assess its impact on humans or wildlife health.
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a novel coltiVirus related Virus isolated from free tailed bats from cote d ivoire is able to infect human cells in vitro
Virology Journal, 2017Co-Authors: Sabrina Weiss, Piotr Wojtek Dabrowski, Andreas Kurth, Siv Aina J. Leendertz, Fabian H. LeendertzAbstract:Zoonotic transmission events play a major role in the emergence of novel diseases. While such events are virtually impossible to predict, wildlife screening for potential emerging pathogens can be a first step. Driven by recent disease epidemics like severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Ebola, bats have gained special interest as reservoirs of emerging Viruses. As part of a bigger study investigating pathogens in African bats we screened animals for the presence of known and unknown Viruses. We isolated and characterised a novel reoVirus from blood of free-tailed bats (Chaereophon aloysiisabaudiae) captured in 2006 in Cote d’Ivoire. The Virus showed closest relationship with two human pathogenic Viruses, Colorado tick fever Virus and Eyach Virus, and was able to infect various human cell lines in vitro. The study shows the presence of a coltiVirus-related Virus in bats from Sub-Sahara Africa. Serological studies could help to assess its impact on humans or wildlife health.
Sabrina Weiss - One of the best experts on this subject based on the ideXlab platform.
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A novel ColtiVirus-related Virus isolated from free-tailed bats from Côte d’Ivoire is able to infect human cells in vitro
Virology Journal, 2017Co-Authors: Sabrina Weiss, Piotr Wojtek Dabrowski, Andreas Kurth, Siv Aina J. Leendertz, Fabian H. LeendertzAbstract:Background Zoonotic transmission events play a major role in the emergence of novel diseases. While such events are virtually impossible to predict, wildlife screening for potential emerging pathogens can be a first step. Driven by recent disease epidemics like severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Ebola, bats have gained special interest as reservoirs of emerging Viruses. Methods As part of a bigger study investigating pathogens in African bats we screened animals for the presence of known and unknown Viruses. Results We isolated and characterised a novel reoVirus from blood of free-tailed bats ( Chaereophon aloysiisabaudiae ) captured in 2006 in Côte d’Ivoire. The Virus showed closest relationship with two human pathogenic Viruses, Colorado tick fever Virus and Eyach Virus, and was able to infect various human cell lines in vitro. Conclusion The study shows the presence of a coltiVirus-related Virus in bats from Sub-Sahara Africa. Serological studies could help to assess its impact on humans or wildlife health.
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a novel coltiVirus related Virus isolated from free tailed bats from cote d ivoire is able to infect human cells in vitro
Virology Journal, 2017Co-Authors: Sabrina Weiss, Piotr Wojtek Dabrowski, Andreas Kurth, Siv Aina J. Leendertz, Fabian H. LeendertzAbstract:Zoonotic transmission events play a major role in the emergence of novel diseases. While such events are virtually impossible to predict, wildlife screening for potential emerging pathogens can be a first step. Driven by recent disease epidemics like severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Ebola, bats have gained special interest as reservoirs of emerging Viruses. As part of a bigger study investigating pathogens in African bats we screened animals for the presence of known and unknown Viruses. We isolated and characterised a novel reoVirus from blood of free-tailed bats (Chaereophon aloysiisabaudiae) captured in 2006 in Cote d’Ivoire. The Virus showed closest relationship with two human pathogenic Viruses, Colorado tick fever Virus and Eyach Virus, and was able to infect various human cell lines in vitro. The study shows the presence of a coltiVirus-related Virus in bats from Sub-Sahara Africa. Serological studies could help to assess its impact on humans or wildlife health.
