The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Ruben H Sambuelli - One of the best experts on this subject based on the ideXlab platform.
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basic and clinical immunology 3029 secretory leukocyte protease inhibitor plays an important role in the regulation of Eye Inflammation and wound healing
World Allergy Organization Journal, 2013Co-Authors: Andres Ricardo Grenat, V E Reviglio, Ruben H SambuelliAbstract:Methods We used immunohistochemistry to localize SLPI and MMPs in normal and inflamed-infected rat Eye tissues. The expression levels of SLPI mRNA protein were evaluated by RT-PCR and western blotting. Eighty female Lewis rats (200g) were randomly divided into four test groups as follows: 1) Staphylococcus aureus endophthalmitis, 2) S.a. keratitis, 3) HSV keratitis, 4) Photorefractive keratectomy. Forty eight hours post-injury or infection, the animals were sacrificed and the Eyes processed for the experimental studies. The values of SLPI bands from western blots were quantified by densitometry analysis and subjected to statistical analysis (p 0.05, Mann-Whitney test) to determine whether there was a difference between the treatment and the control Eye groups.
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Basic and clinical immunology – 3029. Secretory leukocyte protease inhibitor plays an important role in the regulation of Eye Inflammation and wound healing
World Allergy Organization Journal, 2013Co-Authors: Andres Ricardo Grenat, V E Reviglio, Ruben H SambuelliAbstract:Methods We used immunohistochemistry to localize SLPI and MMPs in normal and inflamed-infected rat Eye tissues. The expression levels of SLPI mRNA protein were evaluated by RT-PCR and western blotting. Eighty female Lewis rats (200g) were randomly divided into four test groups as follows: 1) Staphylococcus aureus endophthalmitis, 2) S.a. keratitis, 3) HSV keratitis, 4) Photorefractive keratectomy. Forty eight hours post-injury or infection, the animals were sacrificed and the Eyes processed for the experimental studies. The values of SLPI bands from western blots were quantified by densitometry analysis and subjected to statistical analysis (p 0.05, Mann-Whitney test) to determine whether there was a difference between the treatment and the control Eye groups.
James T. Rosenbaum - One of the best experts on this subject based on the ideXlab platform.
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Future for biological therapy for uveitis
Current Opinion in Ophthalmology, 2010Co-Authors: James T. RosenbaumAbstract:PURPOSE OF REVIEW: To review biological therapies as they pertain to the treatment of inflammatory Eye diseases, especially uveitis. RECENT FINDINGS: Biological therapies including antibodies, soluble receptors, and cytokines are being tested increasingly for a variety of ocular Inflammations. As a class, tumor necrosis factor inhibitors have arguably been the most widely employed and have emerged as a successful approach to treat Behcet's disease. Alpha interferon has demonstrated efficacy in the treatment of Behcet's disease and other forms of posterior uveitis. Additional cytokines, cell surface markers, adhesion molecules, and accessory molecules are targets of biological therapy, but the relevance of these targets in Eye Inflammation is sometimes just theoretical. And any disruption of the immune response entails potential risk. SUMMARY: Biological therapies offer tremendous potential in the treatment of ocular Inflammation, but their study to date has been limited and both the efficacy and the risk are incompletely known for most of the available interventions.
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Neurological concomitants of uveitis.
The British journal of ophthalmology, 2004Co-Authors: Justine R. Smith, James T. RosenbaumAbstract:Aim: To describe the prevalence and types of neurological disease that occur in association with uveitis. Methods: Retrospective review of medical records of patients attending a tertiary referral uveitis service over a 15 year period. Results: Of 1450 patients with uveitis, 115 (7.9%) had neurological disease that was considered to be causally related to the Eye Inflammation. The most frequent neurological associations were Vogt-Koyanagi-Harada disease, primary central nervous system lymphoma, multiple sclerosis, and herpes virus infections. Conclusions: Neurological disease is common among patients attending a uveitis service. The distinctive characteristics of the uveal Inflammation may be useful in diagnosing the neurological disease.
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Tumor necrosis factor-alpha is not essential in endotoxin induced Eye Inflammation: studies in cytokine receptor deficient mice.
The Journal of rheumatology, 1998Co-Authors: James T. Rosenbaum, Young Bok Han, Jong Moon Park, Michael Kennedy, Stephen R. PlanckAbstract:OBJECTIVE Anterior uveitis frequently occurs in association with specific systemic inflammatory diseases. Interleukin 1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) have been implicated in the pathogenesis of these diseases. We evaluate the need for these cytokines in a model of anterior uveitis. METHODS Endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I, TNF receptors p55 and p75, both IL-1R1 and TNFR p55, or controls. Eyes were harvested after 24 h for histology and IL-6 bioassays or after 3 h for reverse transcriptase-polymerase chain reaction analysis of mRNA for specific cytokines or enzymes. RESULTS No significant difference in the number of infiltrating cells was found in TNFR p55/p75 deficient mice compared to controls in any of 4 separate experiments or in the combined data (p = 0.8). The number of infiltrating cells was significantly reduced in 2 of 4 experiments with IL-1R1 deficient mice (p < 0.001 based on combined data from 4 studies). IL-1R1/TNFR p55 deficient mice had a reduction in infiltrating cells in 2 of 3 experiments (p < 0.001 based on combined data from all studies). IL-6 levels were not significantly reduced in either of 2 experiments with TNFR p55/p75 deficient mice, but were reduced in one of 2 experiments with IL-1R1-/- mice (p = 0.02) and in one experiment with IL-1R1/TNFR p55 deficient mice (p = 0.01). In response to endotoxin, all 3 receptor deficient lines increased mRNA levels for IL-1-alpha, IL-10, TNF-alpha, IL-1 receptor antagonist, and inducible nitric oxide synthase. CONCLUSIONS IL-1 appears to have a more pivotal role in endotoxin induced uveitis than TNF-alpha, although neither cytokine is essential. Deletion of receptors for both cytokines has the most consistent effect, which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant.
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Paradoxical effects of IL-10 in endotoxin-induced uveitis.
Journal of Immunology, 1995Co-Authors: James T. Rosenbaum, Ethan AngellAbstract:Uveitis, or intraocular Inflammation, can be provoked in laboratory rodents by the local or systemic injection of bacterial endotoxin. Many of the inflammatory effects of endotoxin are potentially due to the induction of cytokine synthesis. IL-10 is a cytokine that potently inhibits the synthesis of many cytokines, including IL-1 and TNF-alpha. We have assessed the ability of IL-10 to inhibit endotoxin-induced uveitis in rabbits and mice. The intravitreal injection of 1 micrograms of human recombinant IL-10 was extremely effective in rabbits in reducing the Inflammation produced by the intravitreal injection of 250 ng of Escherichia coli endotoxin, as judged by the reduced accumulation of cells and protein in the aqueous humor. Locally injected IL-10 was similarly effective in blocking the ocular inflammatory effects of intravitreally injected endotoxin in a mouse model. If the injection of IL-10 was delayed subsequent to the endotoxin injection, the reduced inflammatory effects in the rabbit model were diminished. In contrast to its ability to inhibit the local inflammatory effect of endotoxin in the Eye, IL-10 did not reduce the Inflammation induced by a local ocular injection of 400 U of human recombinant IL-alpha. Paradoxically, in a mouse model of uveitis subsequent to intraperitoneally injected endotoxin, the simultaneous injection of 1 micrograms of IL-10 and endotoxin potentiated the ocular Inflammation, as judged by the number of leukocytes seen in histologic sections. This effect was dose dependent, since Eye Inflammation was markedly inhibited by 100 micrograms of IL-10 injected i.p. These observations are compatible with the hypothesis that locally injected IL-10 acts by reducing cytokine synthesis in these uveitis models. Intraperitoneally injected IL-10 can either inhibit or suppress endotoxin-induced Eye Inflammation in a dose-dependent manner.
Andres Ricardo Grenat - One of the best experts on this subject based on the ideXlab platform.
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basic and clinical immunology 3029 secretory leukocyte protease inhibitor plays an important role in the regulation of Eye Inflammation and wound healing
World Allergy Organization Journal, 2013Co-Authors: Andres Ricardo Grenat, V E Reviglio, Ruben H SambuelliAbstract:Methods We used immunohistochemistry to localize SLPI and MMPs in normal and inflamed-infected rat Eye tissues. The expression levels of SLPI mRNA protein were evaluated by RT-PCR and western blotting. Eighty female Lewis rats (200g) were randomly divided into four test groups as follows: 1) Staphylococcus aureus endophthalmitis, 2) S.a. keratitis, 3) HSV keratitis, 4) Photorefractive keratectomy. Forty eight hours post-injury or infection, the animals were sacrificed and the Eyes processed for the experimental studies. The values of SLPI bands from western blots were quantified by densitometry analysis and subjected to statistical analysis (p 0.05, Mann-Whitney test) to determine whether there was a difference between the treatment and the control Eye groups.
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Basic and clinical immunology – 3029. Secretory leukocyte protease inhibitor plays an important role in the regulation of Eye Inflammation and wound healing
World Allergy Organization Journal, 2013Co-Authors: Andres Ricardo Grenat, V E Reviglio, Ruben H SambuelliAbstract:Methods We used immunohistochemistry to localize SLPI and MMPs in normal and inflamed-infected rat Eye tissues. The expression levels of SLPI mRNA protein were evaluated by RT-PCR and western blotting. Eighty female Lewis rats (200g) were randomly divided into four test groups as follows: 1) Staphylococcus aureus endophthalmitis, 2) S.a. keratitis, 3) HSV keratitis, 4) Photorefractive keratectomy. Forty eight hours post-injury or infection, the animals were sacrificed and the Eyes processed for the experimental studies. The values of SLPI bands from western blots were quantified by densitometry analysis and subjected to statistical analysis (p 0.05, Mann-Whitney test) to determine whether there was a difference between the treatment and the control Eye groups.
Chien-yun Hsiang - One of the best experts on this subject based on the ideXlab platform.
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The protective effect of quercetin on retinal Inflammation in mice: the involvement of tumor necrosis factor/nuclear factor-κB signaling pathways.
Food & function, 2020Co-Authors: I-chen Liu, Ken-an Lin, Yi-fang Liao, Chien-yun HsiangAbstract:Quercetin is a natural flavonoid that occurs in fruits and vegetables. Retinal Inflammation is an important cause of vision loss. This study was aimed to analyze the effects of oral administration of quercetin on retinal Inflammation. Transgenic mice, carrying nuclear factor-κB (NF-κB)-driven luciferase genes, were injected with 1 mg per kg body weight of lipopolysaccharide (LPS). Various amounts (1, 10, and 100 mg per kg body weight) of quercetin were orally given to mice. LPS-induced retinal Inflammation was evaluated by bioluminescence imaging and histological examination 4 hours later. RNA-Seq analysis of gene expression profiles was performed to explain the mechanisms of quercetin on Eye Inflammation. Our data showed that LPS enhanced luminescent signals on ocular tissues, while LPS-induced luminescence intensities were significantly suppressed by quercetin by 73.61 ± 21.74%. LPS significantly increased the thickness of retinal tissues by 1.52 ± 0.37 fold, in comparison with the mock, while quercetin reduced the LPS-induced retinal thickness and decreased the accumulation of infiltrating granulocytes. Biological pathway analysis showed that tumor necrosis factor (TNF), cytokine, and NF-κB signaling pathways were involved in the anti-inflammatory mechanisms of quercetin. Immunohistochemical staining further showed that quercetin reduced the activation of NF-κB, the expression of interleukin-1β and TNF-α, and the infiltration of granulocytes in retinal tissues. In conclusion, this is the first study reporting the effects and mechanisms of orally administered quercetin against LPS-induced retinal Inflammation in mice. Due to its safety, our study suggested that supplementation of quercetin has beneficial effects on the Eyes.
Dusan Ferluga - One of the best experts on this subject based on the ideXlab platform.
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hypocomplementemic urticarial vasculitis syndrome a case report
Pathology, 2014Co-Authors: A Vizjak, Jelka Lindic, Jerica Mraz, Dusan FerlugaAbstract:Hypocomplementemic urticarial vasculitis syndrome (HUVS) is a rare immune-complex small vessel systemic vasculitis involving kidney in about 50% of patients. Characteristic clinical features are recurrent urticaria, decreased serum complement, skin vasculitis, arthritis, Eye Inflammation, abdominal pain, glomerulonephritis and positive anti-C1q antibodies. We report a 36-year-old woman with a 12-year history of recurrent urticaria and 2 years of urticarial vasculitis. On admission, she also had arthritis, pleuritis and pericarditis, abdominal pain, pathologic urinalysis, low serum values of C3, C4 and CH50. All immunoserology was negative except for ANA 1:80 and anti-C1q 620 IU. ’Full-house’ immune complex vasculitis was demonstrated in skin and lung biopsies. A kidney biopsy showed diffuse proliferative segmental active and sclerosing glomerulonephritis with mesangial and endocapillary proliferation, neutrophil exudation, necrosis, extracapillary crescents and segmental and global glomerulosclerosis. ’Full-house’ glomerular and extensive extraglomerular vascular and tubulointerstitial immune deposits with dominant IgG, C3 and C1q were noted by immunofluorescence. Electron microscopy revealed electron dense deposits with an ultrastructural ’fingerprint’ pattern in the glomerular mesangium, transmembranous in capillary walls and in the arteriolar wall. Our patient fulfilled the criteria for HUVS and SLE, in accordance with the not infrequent clinical and immunoserological overlapping of the two diseases.
